FvH4_4g04310

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000555
Physical Location & Seq
Forward (+)
3698430 .. 3706269
7840 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04310.t5

Sequence Viewer

Length: 2598 bp
ATGAGAGTGGCAGTAGTTGGTTCTGGTATCAGTGGTTTGGTCGCAGCCTATGTTCTTGCAAAAGATGGGGTGGAAGTGGATCTGTATGAGAAAGAAGACTACTTGGGAGGTCATGCCAGGACGGTCACATTCGACGGCGTCGATTTGGATCTTGGTTTCATGGTCTTCAATAGAGTAACATATCCCAACATGATGGAATTCTTTGAGAGTCTTGGAGTTGAGATGGAGATATCTGACATGTCCTTCTCGGCAAGCTTGGACAAAGGAAAGGGCTACGAATGGGGTAGTCGAAATGGTCTGTCGAGCTTGTTTGCGCAAAAGAGCAATGTGTTGAATCCATACTTTTGGCAAATGCTTCGAGAAATCACCAAGTTTAAACATGATGCTCTCAGTTATCTTGAGGTGCGCGAGAACAATCCGGACATTGATCGAAATGAAACCTTGGGGAAGTTTATCGAGTCCCGTCACTACTCCGAATTATTTCAGAAGGCTTATCTTGTTCCAATATGTGGTTCAATCTGGTCATGCCCTGCAGAAGGGGTTATGAGCTTCTCAGCCTTCTCTGTTCTATCGTTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCCGGCCACAATGGTTAACTGTCAGATGGCGTTCACATTGTTATGTGAAGAAGGTTAGAGAAGTGTTGGAGAGTAAAGGGTGTCGGATAAGAACAAGTTGTGAGGTACATAAAGTTTCCACAAACGATGAGGGTTCCACAGTAGTCTCTGGAGATGAGTTTGAGGAAATATACAATGGATGCATAATGGCTGTCCACGCCCCCGACGCCGTGAGAATATTAGGAGACCAGGCAACATCAGATGAACTGAGAGTACTTGGTGCCTTCCAATATGTCTACAGTGATATTTTCCTCCATCGTGACAAAGATTTAATGCCCCAAAACCCGGCAGCATGGAGTGCATGGAATTTTCTTGGTAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATGTGCTTCAGAACCTTGGTGAAACCAGTCTACCATTTCTTGTGACTCTCAATCCGGATCATACACCAAAACATACCTTGCTTAAGTGGTCAACTACCCATCCAATCCCATCTGTTGCTGCATCAAAAGCTGCACTTGAGCTTCCTCATATTCAAGGAAAGAGAGGAATCTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGATTAAAGGCTGGGATGGCTGCAGCACATGGTATTCTTGGTAACAATTGTGCCCTCTTGAGCAACCCGAAACACATGGTTCCGTCATTGACAGAAACTGGAGCACGCCTTTTCGTTACTAGATTTATAAGACATTATATCTCTACTGGATGTTTAATCTTACTAGAGGAAGGGGGCACAATGTTCACCTTTGAAGGAACAAGAAAAGGGTGTTCTTTAAAATGTGTTCTTAAAGTTCATAATCCTCAGTTTTACTGGAAGGTTATGACTCAGGCTGATTTAGGCCTTGCAGATGCATATATCAATGGAGATTTCTCTTTTGTTGACAAAGACAGAGGCCTTTTAAATCTTTTTATGATTCTCATCGCCAACAGAGATAATGATTCTTCCGACTCAAAATTGATTAAGAAAAGGGGCTGGTGGACTCCAATGTTATTCACAGCTAGTATAGCCTCAGCGAAATATTTCTTTCAGCATGTTTCAAGACAAAATACTCTTACACAGGCTCGGAGGAACATCTCTCGTCATTATGACTTGAGTAATGAACTATTTTCGTTGTTCTTGGATGAAACGATGACGTACTCTAGTGCTGTATTTAAGACAGAAGATGAAGAGCTAAAGATTGCACAGTTGAGGAAAATCTCAATTCTCATCGAAAAGGCTAAAATTAGTAAGAACCATGAAGTTCTAGAGATTGGCTGCGGTTGGGGAAGTTTGGCTATCGAAGTCGTCAGACAAACGGGGTGCAGATACACTGGTATCACTCTATCACAAGAACAACTCAAGTATGCCCAACAGAAAGTGAAAGATGCTGACCTTCAGGACAGGATTAGATTCCTTCTATGTGACTACCGTCAAATGCCAGCTAACTACAAATGTGACAGAATTATATCATGCGAGATGCTAGAGAGTGTTGGCCATGAATTTATGAACGATTTCTTTGCTTCCTGTGAATCTGTGCTAGCAGAAAATGGGCTTCTTGTTCTGCAGTTCATATCAATACCGGATGAACGCTATGACGAGTACAGACGAAGTTCAGACTTCATAAAGGAATACATTTTTCCTGGTGGTTGTCTACCTTCATTGAGTAGAGTAACATCTGCCATGGTTAACTCGTCCAGGCTCTGTGTGGAGCACATAGAAAATATCGGAATTCATTACTACCAAACACTGAGATACTGGAGAAAAAATTTCTTGGAGAGACAAAGTGAAATTCTTGCTCTTGGATTCAACGAGAAATTTATTCGGACATGGGAATACTATTTTGATTATTGTGCTGCTGGTTTCAAGACGTATACACTTGGGAACTACCAGATTGTATTTTCACGACCTGGCAACGTTCCAGCATTCAGCAATCCCTACAAAGGCTTCCCTTCTGCATATTGA

Protein Analysis

866

Amino Acids

98.55

Weight (kDa)

6.58

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 1.8e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 9e-19 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 275 1.9e-20 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
Acc16I TGCGCA 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 4 cut(s) 882, 1030, 2287, 2507
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 2 cut(s) 418, 1054
AciI CCGC 1 cut(s) 1914
AclI AACGTT 1 cut(s) 2550
AclWI GGATC 3 cut(s) 87, 156, 1065
AcoI YGGCCR 3 cut(s) 607, 611, 2128
AcsI RAATTY 7 cut(s) 197, 952, 2135, 2364, 2401, 2424, 2450
AcuI CTGAAG 3 cut(s) 581, 992, 2015
AcyI GRCGYC 2 cut(s) 138, 813
AfaI GTAC 5 cut(s) 714, 861, 967, 1793, 2237
AfiI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 8 cut(s) 169, 334, 516, 1154, 1406, 1695, 2443, 2500
AjnI CCWGG 6 cut(s) 116, 834, 1188, 2275, 2330, 2542
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
AluBI AGCT 9 cut(s) 255, 306, 549, 601, 1130, 1141, 1655, 1828, 2078
AluI AGCT 9 cut(s) 255, 306, 549, 601, 1130, 1141, 1655, 1828, 2078
Alw21I GWGCWC 2 cut(s) 1318, 2349
Alw26I GTCTC 3 cut(s) 757, 825, 2407
AlwI GGATC 3 cut(s) 87, 156, 1065
AlwNI CAGNNNCTG 2 cut(s) 1310, 2337
Aor13HI TCCGGA 2 cut(s) 418, 1054
AoxI GGCC 5 cut(s) 607, 611, 1495, 1549, 2128
ApeKI GCWGC 8 cut(s) 44, 935, 1118, 1130, 1232, 1235, 1911, 2489
ApoI RAATTY 7 cut(s) 197, 952, 2135, 2364, 2401, 2424, 2450
Asp700I GAANNNNTTC 3 cut(s) 480, 1676, 2147
AspLEI GCGC 2 cut(s) 316, 408
AsuC2I CCSGG 1 cut(s) 932
AsuHPI GGTGA 3 cut(s) 358, 1031, 1390
AsuNHI GCTAGC 1 cut(s) 2173
BaeGI GKGCMC 2 cut(s) 1267, 1391
BaeI ACNNNNGTAYC 4 cut(s) 1954, 1954, 1987, 1987
BalI TGGCCA 1 cut(s) 2130
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 102, 157
Bbv12I GWGCWC 2 cut(s) 1318, 2349
BbvCI CCTCAGC 1 cut(s) 1666
BbvI GCAGC 8 cut(s) 56, 947, 1105, 1117, 1219, 1247, 1898, 2476
BccI CCATC 9 cut(s) 59, 187, 217, 627, 909, 1107, 1117, 1205, 1222
BceAI ACGGC 2 cut(s) 151, 800
BcgI CGANNNNNNTGC 4 cut(s) 338, 372, 2135, 2169
BciT130I CCWGG 6 cut(s) 118, 836, 1190, 2277, 2332, 2544
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 3 cut(s) 757, 825, 2407
BfaI CTAG 7 cut(s) 1332, 1376, 1656, 1797, 1901, 2117, 2174
BfmI CTRYAG 4 cut(s) 531, 883, 1233, 2198
BfrI CTTAAG 1 cut(s) 1082
BisI GCNGC 8 cut(s) 45, 936, 1119, 1131, 1233, 1236, 1912, 2490
BlsI GCNGC 8 cut(s) 46, 937, 1120, 1132, 1234, 1237, 1913, 2491
BmcAI AGTACT 2 cut(s) 861, 967
Bme1390I CCNGG 7 cut(s) 118, 836, 932, 1190, 2277, 2332, 2544
BmiI GGNNCC 3 cut(s) 742, 868, 1293
BmrFI CCNGG 7 cut(s) 118, 836, 932, 1190, 2277, 2332, 2544
BmsI GCATC 6 cut(s) 373, 776, 1130, 1495, 2011, 2103
BmtI GCTAGC 1 cut(s) 2177
BoxI GACNNNNGTC 1 cut(s) 2064
BpiI GAAGAC 2 cut(s) 102, 157
BpmI CTGGAG 3 cut(s) 777, 1332, 2413
Bpu10I CCTNAGC 1 cut(s) 1666
BpuEI CTTGAG 5 cut(s) 419, 1157, 1291, 1768, 1979
BpuMI CCSGG 1 cut(s) 932
BsaBI GATNNNNATC 2 cut(s) 147, 1574
BsaHI GRCGYC 2 cut(s) 138, 813
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 4 cut(s) 441, 1015, 1189, 2316
BsaWI WCCGGW 3 cut(s) 418, 1054, 2215
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 7 cut(s) 998, 1026, 1315, 1363, 1472, 1972, 2396
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 2 cut(s) 147, 1574
BseAI TCCGGA 2 cut(s) 418, 1054
BseBI CCWGG 6 cut(s) 118, 836, 1190, 2277, 2332, 2544
BseDI CCNNGG 4 cut(s) 441, 1015, 1189, 2316
BseGI GGATG 7 cut(s) 791, 1099, 1216, 1233, 1367, 1783, 2224
BseJI GATNNNNATC 2 cut(s) 147, 1574
BseLI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 7 cut(s) 403, 567, 845, 1472, 1496, 1680, 2375
BseNI ACTGG 7 cut(s) 998, 1026, 1315, 1363, 1472, 1972, 2396
BseSI GKGCMC 2 cut(s) 1267, 1391
BseXI GCAGC 8 cut(s) 56, 947, 1105, 1117, 1219, 1247, 1898, 2476
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 2 cut(s) 1116, 1978
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 5 cut(s) 609, 613, 1497, 1551, 2130
BshNI GGYRCC 1 cut(s) 866
BsiHKAI GWGCWC 2 cut(s) 1318, 2349
BsiSI CCGG 5 cut(s) 419, 610, 932, 1055, 2216
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
BsmAI GTCTC 3 cut(s) 757, 825, 2407
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 2558
BsnI GGCC 5 cut(s) 609, 613, 1497, 1551, 2130
Bso31I GGTCTC 1 cut(s) 825
Bsp1286I GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
Bsp13I TCCGGA 2 cut(s) 418, 1054
Bsp143I GATC 4 cut(s) 79, 148, 427, 1057
Bsp19I CCATGG 1 cut(s) 2316
BspACI CCGC 1 cut(s) 1914
BspANI GGCC 5 cut(s) 609, 613, 1497, 1551, 2130
BspCNI CTCAG 7 cut(s) 402, 566, 846, 1471, 1495, 1679, 2376
BspEI TCCGGA 2 cut(s) 418, 1054
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 3 cut(s) 742, 868, 1293
BspMAI CTGCAG 3 cut(s) 535, 1237, 2202
BspOI GCTAGC 1 cut(s) 2177
BspPI GGATC 3 cut(s) 87, 156, 1065
BspQI GCTCTTC 1 cut(s) 1818
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 825
BsrDI GCAATG 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 7 cut(s) 998, 1026, 1315, 1363, 1472, 1972, 2396
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 4 cut(s) 441, 1015, 1189, 2316
BssMI GATC 4 cut(s) 79, 148, 427, 1057
BssNAI GTATAC 1 cut(s) 2508
BssNI GRCGYC 2 cut(s) 138, 813
BssT1I CCWWGG 3 cut(s) 441, 1015, 2316
Bst1107I GTATAC 1 cut(s) 2508
Bst2UI CCWGG 6 cut(s) 118, 836, 1190, 2277, 2332, 2544
Bst4CI ACNGT 6 cut(s) 124, 628, 748, 887, 1842, 2066
Bst6I CTCTTC 1 cut(s) 1818
BstACI GRCGYC 2 cut(s) 138, 813
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 944
BstC8I GCNNGC 5 cut(s) 253, 611, 1318, 2076, 2175
BstDEI CTNAG 7 cut(s) 389, 553, 854, 1458, 1482, 1666, 2384
BstDSI CCRYGG 1 cut(s) 2316
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 7 cut(s) 791, 1099, 1216, 1233, 1367, 1783, 2224
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 2 cut(s) 316, 408
BstKTI GATC 4 cut(s) 82, 151, 430, 1060
BstMAI GTCTC 3 cut(s) 757, 825, 2407
BstMBI GATC 4 cut(s) 79, 148, 427, 1057
BstMWI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1661
BstNI CCWGG 6 cut(s) 118, 836, 1190, 2277, 2332, 2544
BstNSI RCATGY 2 cut(s) 241, 1691
BstPAI GACNNNNGTC 1 cut(s) 2064
BstSCI CCNGG 7 cut(s) 116, 834, 930, 1188, 2275, 2330, 2542
BstSFI CTRYAG 4 cut(s) 531, 883, 1233, 2198
BstSLI GKGCMC 2 cut(s) 1267, 1391
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 8 cut(s) 56, 947, 1105, 1117, 1219, 1247, 1898, 2476
BstV2I GAAGAC 2 cut(s) 102, 157
BstX2I RGATCY 2 cut(s) 79, 148
BstXI CCANNNNNNTGG 4 cut(s) 193, 345, 1196, 1211
BstYI RGATCY 2 cut(s) 79, 148
BstZ17I GTATAC 1 cut(s) 2508
BsuRI GGCC 5 cut(s) 609, 613, 1497, 1551, 2130
BtgI CCRYGG 1 cut(s) 2316
BtgZI GCGATG 1 cut(s) 1561
BtsCI GGATG 7 cut(s) 791, 1099, 1216, 1233, 1367, 1783, 2224
BtsIMutI CAGTG 4 cut(s) 37, 892, 1965, 2381
Cac8I GCNNGC 5 cut(s) 253, 611, 1318, 2076, 2175
CaiI CAGNNNCTG 2 cut(s) 1310, 2337
CfoI GCGC 2 cut(s) 316, 408
Cfr10I RCCGGY 1 cut(s) 609
CseI GACGC 2 cut(s) 127, 821
Csp6I GTAC 5 cut(s) 713, 860, 966, 1792, 2236
CviQI GTAC 5 cut(s) 713, 860, 966, 1792, 2236
DdeI CTNAG 7 cut(s) 389, 553, 854, 1458, 1482, 1666, 2384
DpnI GATC 4 cut(s) 81, 150, 429, 1059
DpnII GATC 4 cut(s) 79, 148, 427, 1057
DraI TTTAAA 3 cut(s) 376, 1431, 1557
EaeI YGGCCR 3 cut(s) 607, 611, 2128
Eam1104I CTCTTC 1 cut(s) 1818
EarI CTCTTC 1 cut(s) 1818
Eco130I CCWWGG 3 cut(s) 441, 1015, 2316
Eco147I AGGCCT 2 cut(s) 1497, 1551
Eco31I GGTCTC 1 cut(s) 825
Eco32I GATATC 1 cut(s) 231
Eco57I CTGAAG 3 cut(s) 581, 992, 2015
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 2 cut(s) 197, 2364
EcoRII CCWGG 6 cut(s) 116, 834, 1188, 2275, 2330, 2542
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 3 cut(s) 441, 1015, 2316
EcoT22I ATGCAT 2 cut(s) 791, 1510
ErhI CCWWGG 3 cut(s) 441, 1015, 2316
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 1 cut(s) 445
FblI GTMKAC 4 cut(s) 882, 1030, 2287, 2507
Fnu4HI GCNGC 8 cut(s) 45, 936, 1119, 1131, 1233, 1236, 1912, 2490
FokI GGATG 7 cut(s) 798, 1086, 1223, 1240, 1374, 1790, 2231
FseI GGCCGGCC 1 cut(s) 613
Fsp4HI GCNGC 8 cut(s) 45, 936, 1119, 1131, 1233, 1236, 1912, 2490
FspBI CTAG 7 cut(s) 1332, 1376, 1656, 1797, 1901, 2117, 2174
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 2 cut(s) 315, 407
GluI GCNGC 8 cut(s) 45, 936, 1119, 1131, 1233, 1236, 1912, 2490
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 3 cut(s) 777, 1332, 2413
HaeIII GGCC 5 cut(s) 609, 613, 1497, 1551, 2130
HapII CCGG 5 cut(s) 419, 610, 932, 1055, 2216
HgaI GACGC 2 cut(s) 127, 821
HhaI GCGC 2 cut(s) 316, 408
Hin1I GRCGYC 2 cut(s) 138, 813
Hin6I GCGC 2 cut(s) 314, 406
HinP1I GCGC 2 cut(s) 314, 406
HincII GTYRAC 4 cut(s) 624, 1092, 1537, 2323
HindII GTYRAC 4 cut(s) 624, 1092, 1537, 2323
HindIII AAGCTT 1 cut(s) 253
HpaI GTTAAC 2 cut(s) 624, 2323
HpaII CCGG 5 cut(s) 419, 610, 932, 1055, 2216
HphI GGTGA 3 cut(s) 358, 1031, 1390
Hpy99I CGWCG 3 cut(s) 137, 143, 815
HpyCH4III ACNGT 6 cut(s) 124, 628, 748, 887, 1842, 2066
HpyCH4IV ACGT 3 cut(s) 1790, 2504, 2550
HpyF10VI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1661
HpyF3I CTNAG 7 cut(s) 389, 553, 854, 1458, 1482, 1666, 2384
HpySE526I ACGT 3 cut(s) 1790, 2504, 2550
Hsp92I GRCGYC 2 cut(s) 138, 813
HspAI GCGC 2 cut(s) 314, 406
Kpn2I TCCGGA 2 cut(s) 418, 1054
KroI GCCGGC 1 cut(s) 609
KroNI GCCGGC 1 cut(s) 611
KspAI GTTAAC 2 cut(s) 624, 2323
Kzo9I GATC 4 cut(s) 79, 148, 427, 1057
LguI GCTCTTC 1 cut(s) 1818
LmnI GCTCC 3 cut(s) 1181, 1313, 2344
Lsp1109I GCAGC 8 cut(s) 56, 947, 1105, 1117, 1219, 1247, 1898, 2476
LweI GCATC 6 cut(s) 373, 776, 1130, 1495, 2011, 2103
MaeI CTAG 7 cut(s) 1332, 1376, 1656, 1797, 1901, 2117, 2174
MaeII ACGT 3 cut(s) 1790, 2504, 2550
MalI GATC 4 cut(s) 81, 150, 429, 1059
MboI GATC 4 cut(s) 79, 148, 427, 1057
MboII GAAGA 6 cut(s) 107, 157, 667, 1590, 1829, 1835
MfeI CAATTG 1 cut(s) 1258
MflI RGATCY 2 cut(s) 79, 148
MhlI GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
MlsI TGGCCA 1 cut(s) 2130
MluNI TGGCCA 1 cut(s) 2130
MlyI GAGTC 6 cut(s) 217, 467, 1039, 1474, 1598, 1630
MmeI TCCRAC 3 cut(s) 654, 671, 1626
Mox20I TGGCCA 1 cut(s) 2130
Mph1103I ATGCAT 2 cut(s) 791, 1510
MroI TCCGGA 2 cut(s) 418, 1054
MroNI GCCGGC 1 cut(s) 609
MroXI GAANNNNTTC 3 cut(s) 480, 1676, 2147
MscI TGGCCA 1 cut(s) 2130
MslI CAYNNNNRTG 2 cut(s) 648, 1209
Msp20I TGGCCA 1 cut(s) 2130
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 5 cut(s) 419, 610, 932, 1055, 2216
MspR9I CCNGG 7 cut(s) 118, 836, 932, 1190, 2277, 2332, 2544
MssI GTTTAAAC 1 cut(s) 376
MunI CAATTG 1 cut(s) 1258
Mva1269I GAATGC 1 cut(s) 2558
MvaI CCWGG 6 cut(s) 118, 836, 1190, 2277, 2332, 2544
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1661
NaeI GCCGGC 1 cut(s) 611
NciI CCSGG 1 cut(s) 932
NcoI CCATGG 1 cut(s) 2316
NdeII GATC 4 cut(s) 79, 148, 427, 1057
NgoMIV GCCGGC 1 cut(s) 609
NheI GCTAGC 1 cut(s) 2173
NlaIV GGNNCC 3 cut(s) 742, 868, 1293
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 2 cut(s) 791, 1510
NspI RCATGY 2 cut(s) 241, 1691
PceI AGGCCT 2 cut(s) 1497, 1551
PciI ACATGT 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 1818
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 2558
PdiI GCCGGC 1 cut(s) 611
PdmI GAANNNNTTC 3 cut(s) 480, 1676, 2147
PfeI GAWTC 7 cut(s) 334, 1167, 1570, 1595, 2046, 2165, 2439
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 8 cut(s) 46, 937, 1120, 1132, 1234, 1237, 1913, 2491
PleI GAGTC 6 cut(s) 216, 466, 1039, 1474, 1598, 1630
PmeI GTTTAAAC 1 cut(s) 376
PpsI GAGTC 6 cut(s) 216, 466, 1039, 1474, 1598, 1630
PscI ACATGT 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 2064
PsiI TTATAA 1 cut(s) 1340
Psp1406I AACGTT 1 cut(s) 2550
Psp6I CCWGG 6 cut(s) 116, 834, 1188, 2275, 2330, 2542
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 6 cut(s) 116, 834, 1188, 2275, 2330, 2542
PspN4I GGNNCC 3 cut(s) 742, 868, 1293
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstI CTGCAG 3 cut(s) 535, 1237, 2202
PstNI CAGNNNCTG 2 cut(s) 1310, 2337
PsuI RGATCY 2 cut(s) 79, 148
PsyI GACNNNGTC 1 cut(s) 137
RigI GGCCGGCC 1 cut(s) 613
RsaI GTAC 5 cut(s) 714, 861, 967, 1793, 2237
RsaNI GTAC 5 cut(s) 713, 860, 966, 1792, 2236
RseI CAYNNNNRTG 2 cut(s) 648, 1209
SapI GCTCTTC 1 cut(s) 1818
SatI GCNGC 8 cut(s) 45, 936, 1119, 1131, 1233, 1236, 1912, 2490
Sau3AI GATC 4 cut(s) 79, 148, 427, 1057
ScaI AGTACT 2 cut(s) 861, 967
SchI GAGTC 6 cut(s) 217, 467, 1039, 1474, 1598, 1630
ScrFI CCNGG 7 cut(s) 118, 836, 932, 1190, 2277, 2332, 2544
SduI GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
SfaNI GCATC 6 cut(s) 373, 776, 1130, 1495, 2011, 2103
SfcI CTRYAG 4 cut(s) 531, 883, 1233, 2198
SmiMI CAYNNNNRTG 2 cut(s) 648, 1209
SmlI CTYRAG 6 cut(s) 398, 1082, 1136, 1270, 1747, 1994
SmoI CTYRAG 6 cut(s) 398, 1082, 1136, 1270, 1747, 1994
SseBI AGGCCT 2 cut(s) 1497, 1551
SsiI CCGC 1 cut(s) 1914
SspI AATATT 2 cut(s) 825, 1676
SspMI CTAG 7 cut(s) 1332, 1376, 1656, 1797, 1901, 2117, 2174
StuI AGGCCT 2 cut(s) 1497, 1551
StyD4I CCNGG 7 cut(s) 116, 834, 930, 1188, 2275, 2330, 2542
StyI CCWWGG 3 cut(s) 441, 1015, 2316
TaaI ACNGT 6 cut(s) 124, 628, 748, 887, 1842, 2066
TaiI ACGT 3 cut(s) 1793, 2507, 2553
TaqI TCGA 9 cut(s) 132, 141, 289, 302, 358, 430, 456, 1866, 1935
TatI WGTACW 3 cut(s) 859, 965, 2235
TfiI GAWTC 7 cut(s) 334, 1167, 1570, 1595, 2046, 2165, 2439
TscAI CASTG 4 cut(s) 37, 892, 1972, 2388
TseFI GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
TseI GCWGC 8 cut(s) 44, 935, 1118, 1130, 1232, 1235, 1911, 2489
Tsp45I GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
TspGWI ACGGA 1 cut(s) 1284
TspRI CASTG 4 cut(s) 37, 892, 1972, 2388
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 1082
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 7 cut(s) 197, 952, 2135, 2364, 2401, 2424, 2450
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 2 cut(s) 241, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 2338
XmiI GTMKAC 4 cut(s) 882, 1030, 2287, 2507
XmnI GAANNNNTTC 3 cut(s) 480, 1676, 2147
XspI CTAG 7 cut(s) 1332, 1376, 1656, 1797, 1901, 2117, 2174
ZrmI AGTACT 2 cut(s) 861, 967
Zsp2I ATGCAT 2 cut(s) 791, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.