Rh1DG299300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000555
Physical Location & Seq
Forward (+)
52464268 .. 52483367
19100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG299300.1

Sequence Viewer

Length: 1215 bp
ATGTCTGCAGCAAATTCTATGCTTGGAAACGGTTGTACGCTCTTGGAAATGGTCGCACGCCTGTTTGTCACTAGATTTCTAAGACAATATACCTCTACAGGATGCTTCATTTTAATAGAGGAAGACGGCGCAATGTTAACCTTTGAGGGAACGAGCGTCGGCTGTTCTTTAAAATCTATTCTTAAAGTTCACAATCCTCAGTTTTACTGGAAGGTAATGACTCGGTCTGATTTAGGCCTTGCAGATGCATACATCAATGGAGATTTTTCTTTCAATGATAAAGACAAAGGTCTTGAGAATCTCTTTTCGATTCTCATTGCCAATAGAGATTCAAATTCTTCCCTCTCAAACATGAGCAAGAAAAGGGGATGGTGGACGCCATTGTTATATGCAGCTAGTATAACCCCAGCACGATATTTCTTTCATCACATTTCAAGGCAAAACACTCTCACGCAGGCTCGGAGGAACATCTCTCGCCATTATGACCTGGCTAGAATTAATAAGAACCAAGAAGTCCTTGAGATTGGCTGTGGTTGGGGCAGCTTCGCTATTGAAGTGGTCAAGCAAACTGGTTGTAGATACACTGGCATTACTTTGTCCGAGGAACAACTGAAATTTGCCGAAAAGAAAGTGAAAGATGCTGGTATTCAGGACTACATCAAGTTTCTGTTGTGTGACTACCGCAAACTGCCTCCGAACTACAAGTATGACAGAATTATTTCTTGTGAGATGCTGGAACACGTCGGCCACAAATTTATGGACGAATTCTTTGGTTGCTGTGAATCTCTCCTAGCAGAAAAAGGGCTTCTTGTTCTACAGTTCACATCTCTACCGGATGAGCGTTATGATGAGTATAGACAAAGTTCAGATTTCATAAATGAATATATTTTTCCTGGTGCATGTATACCTTCGTTAAGTAGGGTAACATCAGCCATGGCTGCTTCTTCTAGACTCAGTGTGGAGCATTTGGAAAATATTGGAATCCATTATTATCGAACGCTTAAATATTGGAGAAAAAATTTCTTGGAGAAACATGATGAAATACTAGCTCTTGGATTTGATGAAAAGTTCATTCGGACATGGGAGTATTATTTTGATTATTGTGCGGCAGGATTCAACTCATGCACGCTTGGAAATTACCAGATTGTGTTTTCTCGTCCTGGTAATGTTCCTGTATTAGGCGACCCCTACAAAGGCTTCCCTTCTGCTTATTGA

Protein Analysis

404

Amino Acids

46.39

Weight (kDa)

7.51

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 146 - 377 1.4e-55 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 163 - 279 1.2e-11 Methyltransferase domain
Methyltransf_31 PF13847 167 - 280 1.7e-11 Methyltransferase domain
Methyltransf_25 PF13649 172 - 268 1.3e-15 Methyltransferase domain
Methyltransf_11 PF08241 173 - 272 8.4e-13 Methyltransferase domain
Methyltransf_12 PF08242 173 - 270 6.9e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 904
AciI CCGC 2 cut(s) 682, 1106
AcoI YGGCCR 1 cut(s) 745
AcsI RAATTY 6 cut(s) 13, 334, 614, 752, 764, 1018
AcyI GRCGYC 1 cut(s) 377
AfaI GTAC 1 cut(s) 37
AfiI CCNNNNNNNGG 2 cut(s) 1178, 1193
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 5 cut(s) 274, 333, 435, 554, 1117
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 3 cut(s) 486, 892, 1159
AluBI AGCT 3 cut(s) 395, 543, 1049
AluI AGCT 3 cut(s) 395, 543, 1049
AoxI GGCC 2 cut(s) 235, 745
ApeKI GCWGC 4 cut(s) 8, 392, 540, 938
ApoI RAATTY 6 cut(s) 13, 334, 614, 752, 764, 1018
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AseI ATTAAT 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 718
AspLEI GCGC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 20, 404, 552, 925
BccI CCATC 1 cut(s) 363
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 488, 894, 1161
BfaI CTAG 6 cut(s) 72, 396, 492, 791, 948, 1046
BfmI CTRYAG 3 cut(s) 6, 96, 815
BisI GCNGC 5 cut(s) 9, 393, 541, 939, 1107
BlsI GCNGC 5 cut(s) 10, 394, 542, 940, 1108
Bme1390I CCNGG 3 cut(s) 488, 894, 1161
BmgBI CACGTC 1 cut(s) 742
BmrFI CCNGG 3 cut(s) 488, 894, 1161
BmsI GCATC 4 cut(s) 92, 235, 628, 720
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 314, 539
BsaHI GRCGYC 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 600, 933
BsaWI WCCGGW 1 cut(s) 832
Bsc4I CCNNNNNNNGG 2 cut(s) 1178, 1193
Bse1I ACTGG 3 cut(s) 212, 574, 589
Bse3DI GCAATG 2 cut(s) 138, 315
BseBI CCWGG 3 cut(s) 488, 894, 1161
BseDI CCNNGG 2 cut(s) 600, 933
BseGI GGATG 3 cut(s) 107, 374, 841
BseLI CCNNNNNNNGG 2 cut(s) 1178, 1193
BseMI GCAATG 2 cut(s) 138, 315
BseMII CTCAG 2 cut(s) 212, 967
BseNI ACTGG 3 cut(s) 212, 574, 589
BseXI GCAGC 4 cut(s) 20, 404, 552, 925
BseYI CCCAGC 1 cut(s) 406
BshFI GGCC 2 cut(s) 237, 747
BsiSI CCGG 1 cut(s) 833
BslI CCNNNNNNNGG 2 cut(s) 1178, 1193
BsnI GGCC 2 cut(s) 237, 747
Bsp19I CCATGG 1 cut(s) 933
BspACI CCGC 2 cut(s) 682, 1106
BspANI GGCC 2 cut(s) 237, 747
BspCNI CTCAG 2 cut(s) 211, 966
BspMAI CTGCAG 1 cut(s) 10
BsrDI GCAATG 2 cut(s) 138, 315
BsrI ACTGG 3 cut(s) 212, 574, 589
BssECI CCNNGG 2 cut(s) 600, 933
BssNAI GTATAC 1 cut(s) 905
BssNI GRCGYC 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 933
Bst1107I GTATAC 1 cut(s) 905
Bst2UI CCWGG 3 cut(s) 488, 894, 1161
Bst4CI ACNGT 2 cut(s) 32, 819
BstACI GRCGYC 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 58, 456, 1127
BstDEI CTNAG 3 cut(s) 80, 198, 953
BstDSI CCRYGG 1 cut(s) 933
BstENI CCTNNNNNAGG 1 cut(s) 1176
BstF5I GGATG 3 cut(s) 107, 374, 841
BstHHI GCGC 1 cut(s) 131
BstMWI GCNNNNNNNGC 1 cut(s) 938
BstNI CCWGG 3 cut(s) 488, 894, 1161
BstNSI RCATGY 1 cut(s) 903
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 486, 892, 1159
BstSFI CTRYAG 3 cut(s) 6, 96, 815
BstV1I GCAGC 4 cut(s) 20, 404, 552, 925
BstV2I GAAGAC 1 cut(s) 129
BstZ17I GTATAC 1 cut(s) 905
BsuRI GGCC 2 cut(s) 237, 747
BtgI CCRYGG 1 cut(s) 933
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 3 cut(s) 107, 374, 841
BtsIMutI CAGTG 2 cut(s) 582, 961
Cac8I GCNNGC 3 cut(s) 58, 456, 1127
CfoI GCGC 1 cut(s) 131
CseI GACGC 2 cut(s) 145, 385
Csp6I GTAC 1 cut(s) 36
CviAII CATG 6 cut(s) 352, 900, 934, 1034, 1080, 1122
CviQI GTAC 1 cut(s) 36
DdeI CTNAG 3 cut(s) 80, 198, 953
DraI TTTAAA 1 cut(s) 171
EaeI YGGCCR 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 933
Eco147I AGGCCT 1 cut(s) 237
EcoNI CCTNNNNNAGG 1 cut(s) 1176
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 486, 892, 1159
EcoT14I CCWWGG 1 cut(s) 933
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 933
FaeI CATG 6 cut(s) 355, 903, 937, 1037, 1083, 1125
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 792, 824
FatI CATG 6 cut(s) 351, 899, 933, 1033, 1079, 1121
FblI GTMKAC 1 cut(s) 904
Fnu4HI GCNGC 5 cut(s) 9, 393, 541, 939, 1107
FokI GGATG 3 cut(s) 114, 381, 848
Fsp4HI GCNGC 5 cut(s) 9, 393, 541, 939, 1107
FspBI CTAG 6 cut(s) 72, 396, 492, 791, 948, 1046
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 9, 393, 541, 939, 1107
GsaI CCCAGC 1 cut(s) 410
HaeIII GGCC 2 cut(s) 237, 747
HapII CCGG 1 cut(s) 833
HgaI GACGC 2 cut(s) 145, 385
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 6 cut(s) 355, 903, 937, 1037, 1083, 1125
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 8 cut(s) 220, 298, 310, 329, 782, 951, 981, 1113
HpaI GTTAAC 1 cut(s) 138
HpaII CCGG 1 cut(s) 833
Hpy166II GTNNAC 5 cut(s) 138, 190, 375, 822, 905
Hpy188I TCNGA 6 cut(s) 229, 462, 601, 696, 868, 1077
Hpy188III TCNNGA 3 cut(s) 293, 650, 948
Hpy8I GTNNAC 5 cut(s) 138, 190, 375, 822, 905
Hpy99I CGWCG 2 cut(s) 161, 746
HpyAV CCTTC 3 cut(s) 205, 918, 1212
HpyCH4III ACNGT 2 cut(s) 32, 819
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 6 cut(s) 8, 242, 248, 392, 899, 1125
HpyF10VI GCNNNNNNNGC 1 cut(s) 938
HpyF3I CTNAG 3 cut(s) 80, 198, 953
HpySE526I ACGT 1 cut(s) 741
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 6 cut(s) 355, 903, 937, 1037, 1083, 1125
HspAI GCGC 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 961
Lsp1109I GCAGC 4 cut(s) 20, 404, 552, 925
LweI GCATC 4 cut(s) 92, 235, 628, 720
MaeI CTAG 6 cut(s) 72, 396, 492, 791, 948, 1046
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 3 cut(s) 67, 674, 922
MboII GAAGA 3 cut(s) 134, 330, 936
MluCI AATT 9 cut(s) 13, 334, 495, 614, 714, 752, 764, 1018, 1135
MlyI GAGTC 2 cut(s) 214, 945
MnlI CCTC 8 cut(s) 103, 112, 139, 207, 353, 456, 595, 702
Mph1103I ATGCAT 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 718
MseI TTAA 7 cut(s) 113, 137, 170, 183, 498, 914, 1002
MspI CCGG 1 cut(s) 833
MspR9I CCNGG 3 cut(s) 488, 894, 1161
MvaI CCWGG 3 cut(s) 488, 894, 1161
MwoI GCNNNNNNNGC 1 cut(s) 938
NcoI CCATGG 1 cut(s) 933
NlaIII CATG 6 cut(s) 355, 903, 937, 1037, 1083, 1125
NmuCI GTSAC 2 cut(s) 67, 674
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 1 cut(s) 903
PceI AGGCCT 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 718
PfeI GAWTC 6 cut(s) 298, 310, 329, 782, 981, 1113
PflFI GACNNNGTC 1 cut(s) 223
PkrI GCNGC 5 cut(s) 10, 394, 542, 940, 1108
PleI GAGTC 2 cut(s) 214, 945
PpsI GAGTC 2 cut(s) 214, 945
PshAI GACNNNNGTC 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 498
Psp6I CCWGG 3 cut(s) 486, 892, 1159
PspFI CCCAGC 1 cut(s) 406
PspGI CCWGG 3 cut(s) 486, 892, 1159
PstI CTGCAG 1 cut(s) 10
PsyI GACNNNGTC 1 cut(s) 223
RsaI GTAC 1 cut(s) 37
RsaNI GTAC 1 cut(s) 36
SaqAI TTAA 7 cut(s) 113, 137, 170, 183, 498, 914, 1002
SatI GCNGC 5 cut(s) 9, 393, 541, 939, 1107
SchI GAGTC 2 cut(s) 214, 945
ScrFI CCNGG 3 cut(s) 488, 894, 1161
SfaNI GCATC 4 cut(s) 92, 235, 628, 720
SfcI CTRYAG 3 cut(s) 6, 96, 815
SmlI CTYRAG 2 cut(s) 293, 518
SmoI CTYRAG 2 cut(s) 293, 518
Sse9I AATT 9 cut(s) 13, 334, 495, 614, 714, 752, 764, 1018, 1135
SseBI AGGCCT 1 cut(s) 237
SsiI CCGC 2 cut(s) 682, 1106
SspI AATATT 2 cut(s) 976, 1007
SspMI CTAG 6 cut(s) 72, 396, 492, 791, 948, 1046
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 3 cut(s) 486, 892, 1159
StyI CCWWGG 1 cut(s) 933
TaaI ACNGT 2 cut(s) 32, 819
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 308, 994
TaqII GACCGA 1 cut(s) 213
TasI AATT 9 cut(s) 13, 334, 495, 614, 714, 752, 764, 1018, 1135
TauI GCSGC 1 cut(s) 1109
TfiI GAWTC 6 cut(s) 298, 310, 329, 782, 981, 1113
Tru1I TTAA 7 cut(s) 113, 137, 170, 183, 498, 914, 1002
Tru9I TTAA 7 cut(s) 113, 137, 170, 183, 498, 914, 1002
TscAI CASTG 2 cut(s) 589, 961
TseFI GTSAC 2 cut(s) 67, 674
TseI GCWGC 4 cut(s) 8, 392, 540, 938
Tsp45I GTSAC 2 cut(s) 67, 674
TspDTI ATGAA 7 cut(s) 97, 413, 862, 894, 1053, 1060, 1077
TspRI CASTG 2 cut(s) 589, 961
Tth111I GACNNNGTC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 498
XagI CCTNNNNNAGG 1 cut(s) 1176
XapI RAATTY 6 cut(s) 13, 334, 614, 752, 764, 1018
XbaI TCTAGA 1 cut(s) 947
XceI RCATGY 1 cut(s) 903
XmiI GTMKAC 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 718
XspI CTAG 6 cut(s) 72, 396, 492, 791, 948, 1046
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.