Rh4DG051300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000555
Physical Location & Seq
Forward (+)
8133588 .. 8142815
9228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG051300.1

Sequence Viewer

Length: 2598 bp
ATGAGAGTGGCAGTGATTGGTTCTGGTATCAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGATGGGGTTGAAGTGGATCTGTATGAGAAAGAAGACTACTTGGGTGGTCATGCCAGGACGGTCACATTTGATGGCGTCGATTTGGACCTTGGTTTCATGGTCTTCAATCGAGTAACATATCCCAACATGATGGAGTTCTTTGAGAGTCTTGGAGTTGAGATGGAGATATCTGACATGTCCTTCTCGGCAAGCTTAGACAAAGGAAAAGGCTGTGAATGGGGTAGTCGAAATGGTCTGTCGAGCTTGTTTGCGCAAAAGAGCAACGTGTTAAATCCATACTTTTGGCAAATGCTTCGAGAAATCACCAAGTTTAAGCATGATGCCATCAGTTATCTTGAGGTGCATGAGAACAATCCGGACATTGATCGAAATGAAACCTTGGGGAAATTTATCGAGTCCCGTCACTACTCCGAATTATTTCAGAAGGCTTATCTTGTTCCAATATGTGGTTCAATCTGGTCATGCCCTGCAGAAGGGGTTATGAGCTTCTCAGCCTTCTCCGTTCTTTCGTTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCCGCCCACAATGGTTAACTGTCAGATGGCGTTCACATTGTTATGTGAAGAAGGTTAGAGAAGTGCTGGAGAGTAAAGGGTGTCGGATAAGAACAGGTTGTGAGGTGCATAAAGTTTCCACAACCGATGAGGGTTCCACAGTAGTCTCTGGAGATGATTTTGAGGAAATATACAATGGATGCATAATGGCTGTCCACGCCCCTGACGCCGTGAGAATATTAGGAGATCAGGCAACATCAGATGAACTGAGAGTACTTGGTGCCTTCCAATATGTATACAGTGATATTTTCCTCCATCGTGACAAATATTTAATGCCCCAGAACCCGGCAGCATGGAGTGCATGGAATTTTCTCGGTAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATGTGCTTCAGAACCTTGGTGAAACAAGTCTACCATTTCTTGTGACTCTCAATCCAGATCATACACCAAAACATACCTTGCTTAAGTGGTCAACAAGCCATCCAATCCCATCTGTTGCTGCATCAAAGGCTACACTTGAGCTTCCTCATATTCAAGGAAAGAGAGGAATCTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGATTAAAGGCTGGAATGACTGTAGCTCATGGTATTCTTGGAAACAGTTGTGCCCTCTTGAGCAACCCGAAACACATGGTTCCATCATTGACAGAAAGTGGGGCACGCCTTTTTGTAACTAGATTTATAAGACATTATATCTCTACTGGATGTTTAATATTATTAGAGGAAGGGGGCACAATGTTCACCTTTGAAGGAACAAGAAAAGGGTGTTCTTTAAAATGTGTTCTTAAAGTTCATAATCCTCAGTTTTACTGGAAGGTAATGACACAGGCTGATCTAGGCCTTGCAGATGCATATATCAACGGAGATTTCTCTTTTGTTGACAAAGACAGAGACCTTCTAAATCTTTTCATGATTCTCATTGCCAACAGAGATAATGATTCTTCTGACTCAAAATTGATTAAGAAAAGGGGATGGTGGACTCCAATGTTGTTCACAGCTAGTATAGCCTCAGCAAAATATTTCTTTCAGCATGTTTCGAGGCAAAATACTCTAACACAGGCTCGGAGGAACATCTCTCGTCATTATGACCTGAGTAATGAACTATTTTCGCTGTTCTTGGACGAAACAATGACGTATTCTAGTGCTGTATTTAAGACAGAAGATGAGGAGCTGAAGATTGCACAGCTAAGGAAAATATCTATTCTCATTGAAAAGGCTAAAATTAGTAAGAACCATGAAGTTCTAGAGATTGGATGTGGTTGGGGAAGCTTGGCTATCGAAGTTGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAGTTTGCACAACAGAAAGTGAAAGATGCTGGCATTCAGGACCAGATCAGATTTCTTTTATGTGACTACCGCCAATTGCCTTCTAATTACAAATGTGACAGAATCATATCGTGCGAGATGCTAGAGAGTGTTGGCCACGAATTTATGAATGATTTCTTTGCTTCCTGTGAATCTGTGCTAGCAGCAAATGGGCTTCTTGTTCTGCAGTTCATATCAATACCGGATGAACGCTATGACGAGTACAGACGAAGTTCAGACTTCATAAAGGAGTACATTTTCCCTGGTGGTTGTCTACCTTCATTGAGTAGAGTAACATCAGCCATGGTTGGTTCTTCCAGGCTCTGTGTGGAGCACTTGGAAAATATCGGAATTCATTACTACCAAACACTGAGATATTGGAGAAAAAATTTCTTGGAGAGACAAAGTGAAATACTAGCTCTTGGATTCAACGAGAAATTTATTCGGACATGGGAATACTATTTTGATTATTGTGCTGCTGGTTTCAAGACACATACACTTGGAAACTACCAGATTGTGTTTTCACGTCCTGGGAATGTTCCAGCATTCAGCAACCCCTACAAAGGCTTCCCTTCTGCATATTGA

Protein Analysis

865

Amino Acids

98.31

Weight (kDa)

6.52

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 2.5e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 1.4e-17 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 275 2e-18 Flavin containing amine oxidoreductase
CMAS PF02353 570 - 842 2.1e-79 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 626 - 740 1.1e-06 Methyltransferase domain
Methyltransf_31 PF13847 628 - 737 1.4e-10 Methyltransferase domain
Methyltransf_25 PF13649 633 - 729 4.1e-15 Methyltransferase domain
Methyltransf_11 PF08241 634 - 733 1.7e-12 Methyltransferase domain
Methyltransf_12 PF08242 634 - 731 1.2e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
Acc16I TGCGCA 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 3 cut(s) 882, 1030, 2287
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 2 cut(s) 610, 2065
AclWI GGATC 1 cut(s) 87
AcoI YGGCCR 2 cut(s) 607, 2128
AcsI RAATTY 6 cut(s) 449, 952, 2135, 2364, 2401, 2450
AcuI CTGAAG 3 cut(s) 581, 992, 1850
AcyI GRCGYC 2 cut(s) 138, 813
AfaI GTAC 4 cut(s) 861, 967, 2237, 2267
AfiI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
AflII CTTAAG 2 cut(s) 1082, 1994
AflIII ACRYGT 2 cut(s) 237, 327
AgsI TTSAA 8 cut(s) 74, 169, 516, 1154, 1406, 1868, 2443, 2500
AjiI CACGTC 1 cut(s) 2540
AjnI CCWGG 5 cut(s) 116, 1188, 2275, 2330, 2542
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
Alw21I GWGCWC 1 cut(s) 2349
Alw26I GTCTC 4 cut(s) 46, 757, 1542, 2407
AlwI GGATC 1 cut(s) 87
AlwNI CAGNNNCTG 1 cut(s) 2337
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 3 cut(s) 607, 1495, 2128
ApeKI GCWGC 4 cut(s) 935, 1118, 2177, 2489
ApoI RAATTY 6 cut(s) 449, 952, 2135, 2364, 2401, 2450
Asp700I GAANNNNTTC 2 cut(s) 480, 2147
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 148, 2035
AsuC2I CCSGG 1 cut(s) 932
AsuHPI GGTGA 3 cut(s) 358, 1031, 1390
AsuNHI GCTAGC 1 cut(s) 2173
AvaII GGWCC 2 cut(s) 148, 2035
BaeGI GKGCMC 3 cut(s) 1267, 1318, 1391
BalI TGGCCA 1 cut(s) 2130
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 102, 157
Bbv12I GWGCWC 1 cut(s) 2349
BbvCI CCTCAGC 1 cut(s) 1666
BbvI GCAGC 4 cut(s) 947, 1105, 2189, 2476
BceAI ACGGC 1 cut(s) 800
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 5 cut(s) 118, 1190, 2277, 2332, 2544
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 4 cut(s) 46, 757, 1542, 2407
BfaI CTAG 8 cut(s) 1332, 1493, 1656, 1797, 1901, 2117, 2174, 2429
BfmI CTRYAG 3 cut(s) 531, 1233, 2198
BfrI CTTAAG 2 cut(s) 1082, 1994
BisI GCNGC 5 cut(s) 610, 936, 1119, 2178, 2490
BlsI GCNGC 5 cut(s) 611, 937, 1120, 2179, 2491
BmcAI AGTACT 2 cut(s) 861, 967
Bme1390I CCNGG 6 cut(s) 118, 932, 1190, 2277, 2332, 2544
Bme18I GGWCC 2 cut(s) 148, 2035
BmgBI CACGTC 1 cut(s) 2540
BmgT120I GGNCC 2 cut(s) 148, 2035
BmiI GGNNCC 3 cut(s) 742, 868, 1293
BmrFI CCNGG 6 cut(s) 118, 932, 1190, 2277, 2332, 2544
BmsI GCATC 7 cut(s) 373, 776, 1130, 1495, 1980, 2011, 2103
BmtI GCTAGC 1 cut(s) 2177
BpiI GAAGAC 2 cut(s) 102, 157
BpmI CTGGAG 2 cut(s) 695, 777
Bpu10I CCTNAGC 2 cut(s) 1666, 1844
BpuEI CTTGAG 3 cut(s) 419, 1157, 1291
BpuMI CCSGG 1 cut(s) 932
BsaHI GRCGYC 2 cut(s) 138, 813
BsaI GGTCTC 2 cut(s) 46, 1542
BsaJI CCNNGG 7 cut(s) 151, 441, 1015, 1189, 2275, 2316, 2543
BsaWI WCCGGW 2 cut(s) 418, 2215
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
Bse1I ACTGG 4 cut(s) 998, 1363, 1472, 1972
Bse3DI GCAATG 1 cut(s) 1575
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 5 cut(s) 118, 1190, 2277, 2332, 2544
BseDI CCNNGG 7 cut(s) 151, 441, 1015, 1189, 2275, 2316, 2543
BseGI GGATG 7 cut(s) 791, 1099, 1216, 1367, 1634, 1916, 2224
BseLI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
BseMI GCAATG 1 cut(s) 1575
BseMII CTCAG 8 cut(s) 57, 567, 845, 1472, 1680, 1739, 1974, 2375
BseNI ACTGG 4 cut(s) 998, 1363, 1472, 1972
BseRI GAGGAG 1 cut(s) 1838
BseSI GKGCMC 3 cut(s) 1267, 1318, 1391
BseXI GCAGC 4 cut(s) 947, 1105, 2189, 2476
BshFI GGCC 3 cut(s) 609, 1497, 2130
BshNI GGYRCC 1 cut(s) 866
BsiHKAI GWGCWC 1 cut(s) 2349
BsiSI CCGG 3 cut(s) 419, 932, 2216
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 4 cut(s) 509, 536, 931, 2576
BsmAI GTCTC 4 cut(s) 46, 757, 1542, 2407
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 2028, 2558
BsnI GGCC 3 cut(s) 609, 1497, 2130
Bso31I GGTCTC 2 cut(s) 46, 1542
Bsp1286I GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
Bsp19I CCATGG 1 cut(s) 2316
BspACI CCGC 2 cut(s) 610, 2065
BspANI GGCC 3 cut(s) 609, 1497, 2130
BspCNI CTCAG 8 cut(s) 56, 566, 846, 1471, 1679, 1740, 1975, 2376
BspEI TCCGGA 1 cut(s) 418
BspHI TCATGA 1 cut(s) 1566
BspLI GGNNCC 3 cut(s) 742, 868, 1293
BspMAI CTGCAG 2 cut(s) 535, 2202
BspOI GCTAGC 1 cut(s) 2177
BspPI GGATC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 2 cut(s) 1082, 1994
BspTNI GGTCTC 2 cut(s) 46, 1542
BsrDI GCAATG 1 cut(s) 1575
BsrI ACTGG 4 cut(s) 998, 1363, 1472, 1972
BssECI CCNNGG 7 cut(s) 151, 441, 1015, 1189, 2275, 2316, 2543
BssMI GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
BssNAI GTATAC 1 cut(s) 883
BssNI GRCGYC 2 cut(s) 138, 813
BssT1I CCWWGG 4 cut(s) 151, 441, 1015, 2316
Bst1107I GTATAC 1 cut(s) 883
Bst2UI CCWGG 5 cut(s) 118, 1190, 2277, 2332, 2544
Bst4CI ACNGT 6 cut(s) 124, 628, 748, 887, 1234, 1259
BstACI GRCGYC 2 cut(s) 138, 813
BstAFI CTTAAG 2 cut(s) 1082, 1994
BstAPI GCANNNNNTGC 1 cut(s) 944
BstC8I GCNNGC 4 cut(s) 253, 1318, 2026, 2175
BstDSI CCRYGG 1 cut(s) 2316
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 7 cut(s) 791, 1099, 1216, 1367, 1634, 1916, 2224
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 6 cut(s) 82, 430, 835, 1060, 1492, 2043
BstMAI GTCTC 4 cut(s) 46, 757, 1542, 2407
BstMBI GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
BstMWI GCNNNNNNNGC 6 cut(s) 803, 812, 944, 1003, 1127, 1661
BstNI CCWGG 5 cut(s) 118, 1190, 2277, 2332, 2544
BstNSI RCATGY 2 cut(s) 241, 1691
BstSCI CCNGG 6 cut(s) 116, 930, 1188, 2275, 2330, 2542
BstSFI CTRYAG 3 cut(s) 531, 1233, 2198
BstSLI GKGCMC 3 cut(s) 1267, 1318, 1391
BstV1I GCAGC 4 cut(s) 947, 1105, 2189, 2476
BstV2I GAAGAC 2 cut(s) 102, 157
BstX2I RGATCY 1 cut(s) 79
BstXI CCANNNNNNTGG 4 cut(s) 193, 345, 1196, 1211
BstYI RGATCY 1 cut(s) 79
BstZ17I GTATAC 1 cut(s) 883
BsuRI GGCC 3 cut(s) 609, 1497, 2130
BtgI CCRYGG 1 cut(s) 2316
BtrI CACGTC 1 cut(s) 2540
BtsCI GGATG 7 cut(s) 791, 1099, 1216, 1367, 1634, 1916, 2224
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 5 cut(s) 18, 37, 892, 1965, 2381
Cac8I GCNNGC 4 cut(s) 253, 1318, 2026, 2175
CaiI CAGNNNCTG 1 cut(s) 2337
CciI TCATGA 1 cut(s) 1566
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 148, 2035
CseI GACGC 2 cut(s) 127, 821
Csp6I GTAC 4 cut(s) 860, 966, 2236, 2266
CviQI GTAC 4 cut(s) 860, 966, 2236, 2266
DpnI GATC 6 cut(s) 81, 429, 834, 1059, 1491, 2042
DpnII GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
DraI TTTAAA 1 cut(s) 1431
EaeI YGGCCR 2 cut(s) 607, 2128
Eco130I CCWWGG 4 cut(s) 151, 441, 1015, 2316
Eco147I AGGCCT 1 cut(s) 1497
Eco31I GGTCTC 2 cut(s) 46, 1542
Eco32I GATATC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 148, 2035
Eco57I CTGAAG 3 cut(s) 581, 992, 1850
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 2364
EcoRII CCWGG 5 cut(s) 116, 1188, 2275, 2330, 2542
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 4 cut(s) 151, 441, 1015, 2316
EcoT22I ATGCAT 2 cut(s) 791, 1510
ErhI CCWWGG 4 cut(s) 151, 441, 1015, 2316
FaqI GGGAC 1 cut(s) 445
FblI GTMKAC 3 cut(s) 882, 1030, 2287
Fnu4HI GCNGC 5 cut(s) 610, 936, 1119, 2178, 2490
FokI GGATG 7 cut(s) 798, 1086, 1223, 1374, 1641, 1923, 2231
Fsp4HI GCNGC 5 cut(s) 610, 936, 1119, 2178, 2490
FspBI CTAG 8 cut(s) 1332, 1493, 1656, 1797, 1901, 2117, 2174, 2429
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 1 cut(s) 315
GluI GCNGC 5 cut(s) 610, 936, 1119, 2178, 2490
GsuI CTGGAG 2 cut(s) 695, 777
HaeIII GGCC 3 cut(s) 609, 1497, 2130
HapII CCGG 3 cut(s) 419, 932, 2216
HgaI GACGC 2 cut(s) 127, 821
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 2 cut(s) 138, 813
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 3 cut(s) 624, 1092, 1537
HindII GTYRAC 3 cut(s) 624, 1092, 1537
HindIII AAGCTT 2 cut(s) 253, 1924
HpaI GTTAAC 1 cut(s) 624
HpaII CCGG 3 cut(s) 419, 932, 2216
HphI GGTGA 3 cut(s) 358, 1031, 1390
Hpy99I CGWCG 1 cut(s) 143
HpyCH4III ACNGT 6 cut(s) 124, 628, 748, 887, 1234, 1259
HpyCH4IV ACGT 3 cut(s) 327, 1790, 2539
HpyF10VI GCNNNNNNNGC 6 cut(s) 803, 812, 944, 1003, 1127, 1661
HpySE526I ACGT 3 cut(s) 327, 1790, 2539
Hsp92I GRCGYC 2 cut(s) 138, 813
HspAI GCGC 1 cut(s) 314
Kpn2I TCCGGA 1 cut(s) 418
KspAI GTTAAC 1 cut(s) 624
Kzo9I GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
LmnI GCTCC 3 cut(s) 1181, 1825, 2344
Lsp1109I GCAGC 4 cut(s) 947, 1105, 2189, 2476
LweI GCATC 7 cut(s) 373, 776, 1130, 1495, 1980, 2011, 2103
MaeI CTAG 8 cut(s) 1332, 1493, 1656, 1797, 1901, 2117, 2174, 2429
MaeII ACGT 3 cut(s) 327, 1790, 2539
MaeIII GTNAC 9 cut(s) 124, 175, 464, 905, 1042, 1327, 2057, 2090, 2305
MalI GATC 6 cut(s) 81, 429, 834, 1059, 1491, 2042
MboI GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
MboII GAAGA 7 cut(s) 107, 157, 667, 1590, 1829, 1843, 2319
MfeI CAATTG 1 cut(s) 2069
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
MlsI TGGCCA 1 cut(s) 2130
MluNI TGGCCA 1 cut(s) 2130
MlyI GAGTC 5 cut(s) 217, 467, 1039, 1598, 1630
MmeI TCCRAC 1 cut(s) 671
Mox20I TGGCCA 1 cut(s) 2130
Mph1103I ATGCAT 2 cut(s) 791, 1510
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 2 cut(s) 480, 2147
MscI TGGCCA 1 cut(s) 2130
MslI CAYNNNNRTG 2 cut(s) 648, 1209
Msp20I TGGCCA 1 cut(s) 2130
MspCI CTTAAG 2 cut(s) 1082, 1994
MspI CCGG 3 cut(s) 419, 932, 2216
MspR9I CCNGG 6 cut(s) 118, 932, 1190, 2277, 2332, 2544
MunI CAATTG 1 cut(s) 2069
Mva1269I GAATGC 2 cut(s) 2028, 2558
MvaI CCWGG 5 cut(s) 118, 1190, 2277, 2332, 2544
MwoI GCNNNNNNNGC 6 cut(s) 803, 812, 944, 1003, 1127, 1661
NciI CCSGG 1 cut(s) 932
NcoI CCATGG 1 cut(s) 2316
NdeII GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
NheI GCTAGC 1 cut(s) 2173
NlaIV GGNNCC 3 cut(s) 742, 868, 1293
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 2 cut(s) 791, 1510
NspI RCATGY 2 cut(s) 241, 1691
PagI TCATGA 1 cut(s) 1566
PceI AGGCCT 1 cut(s) 1497
PciI ACATGT 1 cut(s) 237
PctI GAATGC 2 cut(s) 2028, 2558
PdmI GAANNNNTTC 2 cut(s) 480, 2147
PfeI GAWTC 6 cut(s) 1167, 1570, 1595, 2097, 2165, 2439
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 5 cut(s) 611, 937, 1120, 2179, 2491
PleI GAGTC 5 cut(s) 216, 466, 1039, 1598, 1630
PpsI GAGTC 5 cut(s) 216, 466, 1039, 1598, 1630
PscI ACATGT 1 cut(s) 237
PsiI TTATAA 1 cut(s) 1340
Psp6I CCWGG 5 cut(s) 116, 1188, 2275, 2330, 2542
PspGI CCWGG 5 cut(s) 116, 1188, 2275, 2330, 2542
PspN4I GGNNCC 3 cut(s) 742, 868, 1293
PspPI GGNCC 2 cut(s) 148, 2035
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstI CTGCAG 2 cut(s) 535, 2202
PstNI CAGNNNCTG 1 cut(s) 2337
PsuI RGATCY 1 cut(s) 79
RsaI GTAC 4 cut(s) 861, 967, 2237, 2267
RsaNI GTAC 4 cut(s) 860, 966, 2236, 2266
RseI CAYNNNNRTG 2 cut(s) 648, 1209
SatI GCNGC 5 cut(s) 610, 936, 1119, 2178, 2490
Sau3AI GATC 6 cut(s) 79, 427, 832, 1057, 1489, 2040
Sau96I GGNCC 2 cut(s) 148, 2035
ScaI AGTACT 2 cut(s) 861, 967
SchI GAGTC 5 cut(s) 217, 467, 1039, 1598, 1630
ScrFI CCNGG 6 cut(s) 118, 932, 1190, 2277, 2332, 2544
SduI GDGCHC 4 cut(s) 1267, 1318, 1391, 2349
SfaNI GCATC 7 cut(s) 373, 776, 1130, 1495, 1980, 2011, 2103
SfcI CTRYAG 3 cut(s) 531, 1233, 2198
SinI GGWCC 2 cut(s) 148, 2035
SmiMI CAYNNNNRTG 2 cut(s) 648, 1209
SmlI CTYRAG 5 cut(s) 398, 1082, 1136, 1270, 1994
SmoI CTYRAG 5 cut(s) 398, 1082, 1136, 1270, 1994
SseBI AGGCCT 1 cut(s) 1497
SsiI CCGC 2 cut(s) 610, 2065
SspI AATATT 4 cut(s) 825, 914, 1371, 1676
SspMI CTAG 8 cut(s) 1332, 1493, 1656, 1797, 1901, 2117, 2174, 2429
StuI AGGCCT 1 cut(s) 1497
StyD4I CCNGG 6 cut(s) 116, 930, 1188, 2275, 2330, 2542
StyI CCWWGG 4 cut(s) 151, 441, 1015, 2316
TaaI ACNGT 6 cut(s) 124, 628, 748, 887, 1234, 1259
TaiI ACGT 3 cut(s) 330, 1793, 2542
TaqI TCGA 9 cut(s) 141, 172, 289, 302, 358, 430, 456, 1694, 1935
TatI WGTACW 4 cut(s) 859, 965, 2235, 2265
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 6 cut(s) 1167, 1570, 1595, 2097, 2165, 2439
TscAI CASTG 5 cut(s) 18, 37, 892, 1972, 2388
TseFI GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
TseI GCWGC 4 cut(s) 935, 1118, 2177, 2489
Tsp45I GTSAC 6 cut(s) 124, 464, 905, 1042, 2057, 2090
TspGWI ACGGA 2 cut(s) 553, 1533
TspRI CASTG 5 cut(s) 18, 37, 892, 1972, 2388
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 2 cut(s) 1082, 1994
VpaK11BI GGWCC 2 cut(s) 148, 2035
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 6 cut(s) 449, 952, 2135, 2364, 2401, 2450
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 2 cut(s) 241, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 2338
XmiI GTMKAC 3 cut(s) 882, 1030, 2287
XmnI GAANNNNTTC 2 cut(s) 480, 2147
XspI CTAG 8 cut(s) 1332, 1493, 1656, 1797, 1901, 2117, 2174, 2429
ZrmI AGTACT 2 cut(s) 861, 967
Zsp2I ATGCAT 2 cut(s) 791, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.