RLG00000009659

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000555
Physical Location & Seq
Forward (+)
56100038 .. 56110011
9974 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009659

Sequence Viewer

Length: 2610 bp
ATGAAAGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGTGTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCCAGGACTGTCACATGTGATGGTGTTGATTTGGACCTTGGTTTCATGGTCTTCAATCATGTAAGTACACTGGTGATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGCGACATGTCCTTCTCAGTGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCGAAATAGTGTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCTTGAGAGTAACCCAGACATTGTCCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTCGGAAGGCTTACCTTATTCCAATATGTAGTTCAATCTTGTCGTGTCCTTCAAAAGGAGTTATAAGCTTGTCAGCTTTCTCTGTGCTTTCGGTTTGTCGCAATTATCAACTACTTCAGCTATTTGGCGGCCCACAGTGGCTTACCGTCAAATGGCGTTCACAGTGTTATGTGAAGAAGGTGAAAGAAGTGTTGGAGAGTAAGGGTTGTCAGATAAGAACCGGCTGTGAGGTACATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTTTTGAGGAAATTTACAATGGATGCATTATTGCTATCCAAGCCCCCAAAGCCGTGAGAATATTAGGAGATCAGGCAACGCCTGATGAACTGAGAATACTTGGCGCCTTCAAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTTATGCCCCAAAATCTAGCAGCATGGAGTGCATTAAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGGTTCAGAACCTCAGAGAAACACGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCATAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCTGCATCGAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGGCTGGAGTGGCTGCAGCATATGGTATGCTTGGGAAAGGTTGTGCCCTGTTGGGAAACCCAAAACATATGGCTCTTTCATTGACAGAAACAGTGGCACGCCTATTTGTTACTACATTTCTTAGATATTATATATGTACCGGATGTTTAATCTTATTAGAAGAGGGAGGCACAATGTTGACCTTTGACGGAAGCAGCAAGGACTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACTGCAGGCTGATTTAGGCATTGCAGTTGCATATATCAGTGGCGATTTTTCTTTCAACGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTCACTGCCAACAGAGATTCTAATGCCTCAAAATCAAACAACAAAAGAGGATGGTGGCAGCCTTGGTTTTTCACAGCCACTATAGCCTCAGCCAGATACTTCTTTCAGCATGCTTTTACGCAAAATAGTCTTGCACAGGCTCGAAGGAACATTTCTCATCATTATGATCTGAGTAATGAACTATTTTCGCTATTCTTGGGCGAAACAATGATGTATTCCACTGCAATATTTAAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTCTACTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTAGATATTGGTTGTGGTTGGGGAACCTTAGCTATTGAAGCAGTTAAACAAACGGGCTGCAGATACACCGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATTTCTTGCGAGATGTTAGAACATGTCGGCCATGAATTTATGGATAAGTTCTTTTCTTGCTGTGAGTCTGTACTGGCAAAAAATGGGATTTTTATTCTCCAGTTCATATCTGTGCCGGATGATCGGTATGATGAGTACAGGCAAAATTCAGGCTACTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACCTTCATTAAGTAGGGTAACATCAGCCATGGCTGCTTCTTCTGGACTCTGTGTGGAGCACTTGGAAAACATTGGAATTCATTATTACCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGAGAGACAGAGTGAAATACTCGCTCTTGGATTCAATGAAAATTTCATCCGGACATGGGAATATTATTTTGATTATTTTGCTGCTGGTTTCAAGTTATATACACTTGGAGATTACCAGATTGTGTTTTCACGTCCTGGTAATGCTCTCACATTCAACAATCCATATAAGGGTTTCCCTTCAGCATATTGA

Protein Analysis

870

Amino Acids

98.55

Weight (kDa)

6.8

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 4.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 74 2.7e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 417 1.4e-17 Flavin containing amine oxidoreductase
CMAS PF02353 574 - 839 8e-80 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 619 - 755 4.1e-11 Methyltransferase domain
Methyltransf_31 PF13847 631 - 740 1.2e-15 Methyltransferase domain
MTS PF05175 631 - 734 1.7e-07 Methyltransferase small domain
Methyltransf_25 PF13649 636 - 732 2.6e-18 Methyltransferase domain
Methyltransf_11 PF08241 637 - 736 1.4e-15 Methyltransferase domain
Methyltransf_12 PF08242 637 - 734 1.7e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 878
AccI GTMKAC 1 cut(s) 2299
AccIII TCCGGA 2 cut(s) 441, 2469
AciI CCGC 2 cut(s) 621, 2074
AclWI GGATC 1 cut(s) 2057
AcoI YGGCCR 2 cut(s) 109, 2137
AcsI RAATTY 8 cut(s) 786, 1179, 2006, 2144, 2254, 2376, 2413, 2461
AcuI CTGAAG 3 cut(s) 593, 2024, 2583
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 7 cut(s) 181, 726, 896, 979, 1369, 2181, 2246
AfiI CCNNNNNNNGG 4 cut(s) 548, 645, 2476, 2588
AflIII ACRYGT 4 cut(s) 128, 249, 1037, 2131
AjiI CACGTC 2 cut(s) 1040, 2552
AjnI CCWGG 5 cut(s) 102, 116, 1200, 2287, 2554
AjuI GAANNNNNNNTTGG 6 cut(s) 437, 469, 508, 540, 668, 700
AluBI AGCT 8 cut(s) 318, 561, 569, 613, 1142, 1153, 1852, 1940
AluI AGCT 8 cut(s) 318, 561, 569, 613, 1142, 1153, 1852, 1940
Alw21I GWGCWC 1 cut(s) 2361
Alw26I GTCTC 4 cut(s) 46, 233, 316, 2419
AlwI GGATC 1 cut(s) 2057
Aor13HI TCCGGA 2 cut(s) 441, 2469
AoxI GGCC 3 cut(s) 109, 622, 2137
ApeKI GCWGC 9 cut(s) 947, 1130, 1244, 1247, 1425, 1645, 1965, 2333, 2501
ApoI RAATTY 8 cut(s) 786, 1179, 2006, 2144, 2254, 2376, 2413, 2461
Asp700I GAANNNNTTC 4 cut(s) 78, 341, 492, 2110
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 2 cut(s) 148, 623
AsuHPI GGTGA 3 cut(s) 199, 370, 685
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 1279
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 157, 1559
Bbv12I GWGCWC 1 cut(s) 2361
BbvCI CCTCAGC 1 cut(s) 1675
BbvI GCAGC 9 cut(s) 959, 1117, 1231, 1259, 1437, 1657, 1952, 2320, 2488
BccI CCATC 6 cut(s) 128, 199, 767, 1119, 1217, 1632
BceAI ACGGC 1 cut(s) 812
BciT130I CCWGG 5 cut(s) 104, 118, 1202, 2289, 2556
BciVI GTATCC 1 cut(s) 204
BclI TGATCA 1 cut(s) 399
BcoDI GTCTC 4 cut(s) 46, 233, 316, 2419
BfaI CTAG 3 cut(s) 944, 1884, 1910
BfmI CTRYAG 4 cut(s) 1245, 1496, 1668, 1966
BfoI RGCGCY 1 cut(s) 882
BfuI GTATCC 1 cut(s) 204
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 5 cut(s) 104, 118, 1202, 2289, 2556
Bme18I GGWCC 1 cut(s) 148
BmgBI CACGTC 2 cut(s) 1040, 2552
BmgT120I GGNCC 2 cut(s) 148, 623
BmiI GGNNCC 2 cut(s) 880, 1933
BmrFI CCNGG 5 cut(s) 104, 118, 1202, 2289, 2556
BmsI GCATC 4 cut(s) 788, 1142, 1989, 2020
BoxI GACNNNNGTC 1 cut(s) 1563
BpiI GAAGAC 2 cut(s) 157, 1559
BpmI CTGGAG 4 cut(s) 789, 1169, 1257, 2192
Bpu10I CCTNAGC 3 cut(s) 1675, 1853, 1936
BpuEI CTTGAG 3 cut(s) 298, 431, 440
Bsa29I ATCGAT 1 cut(s) 1890
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 8 cut(s) 103, 151, 223, 453, 1201, 1561, 1649, 2328
BsaWI WCCGGW 4 cut(s) 441, 1370, 2046, 2469
Bsc4I CCNNNNNNNGG 4 cut(s) 548, 645, 2476, 2588
Bse118I RCCGGY 2 cut(s) 713, 1976
Bse1I ACTGG 7 cut(s) 189, 772, 1010, 1115, 1484, 2187, 2209
Bse3DI GCAATG 1 cut(s) 1512
BseAI TCCGGA 2 cut(s) 441, 2469
BseBI CCWGG 5 cut(s) 104, 118, 1202, 2289, 2556
BseCI ATCGAT 1 cut(s) 1890
BseDI CCNNGG 8 cut(s) 103, 151, 223, 453, 1201, 1561, 1649, 2328
BseGI GGATG 8 cut(s) 803, 1111, 1228, 1379, 1643, 2233, 2300, 2466
BseLI CCNNNNNNNGG 4 cut(s) 548, 645, 2476, 2588
BseMI GCAATG 1 cut(s) 1512
BseMII CTCAG 8 cut(s) 57, 273, 857, 1042, 1484, 1689, 1748, 1983
BseNI ACTGG 7 cut(s) 189, 772, 1010, 1115, 1484, 2187, 2209
BseSI GKGCMC 1 cut(s) 1279
BseXI GCAGC 9 cut(s) 959, 1117, 1231, 1259, 1437, 1657, 1952, 2320, 2488
BsgI GTGCAG 1 cut(s) 1830
BshFI GGCC 3 cut(s) 111, 624, 2139
BshNI GGYRCC 1 cut(s) 878
BshVI ATCGAT 1 cut(s) 1890
BsiHKAI GWGCWC 1 cut(s) 2361
BsiSI CCGG 7 cut(s) 442, 714, 1371, 1977, 2047, 2225, 2470
BslI CCNNNNNNNGG 4 cut(s) 548, 645, 2476, 2588
BsmAI GTCTC 4 cut(s) 46, 233, 316, 2419
BsnI GGCC 3 cut(s) 111, 624, 2139
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 1279, 2361
Bsp13I TCCGGA 2 cut(s) 441, 2469
Bsp143I GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
Bsp19I CCATGG 1 cut(s) 2328
BspACI CCGC 2 cut(s) 621, 2074
BspANI GGCC 3 cut(s) 111, 624, 2139
BspCNI CTCAG 8 cut(s) 56, 272, 858, 1041, 1483, 1688, 1749, 1984
BspDI ATCGAT 1 cut(s) 1890
BspEI TCCGGA 2 cut(s) 441, 2469
BspLI GGNNCC 2 cut(s) 880, 1933
BspMAI CTGCAG 3 cut(s) 1249, 1500, 1970
BspPI GGATC 1 cut(s) 2057
BspT107I GGYRCC 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 1512
BsrFI RCCGGY 2 cut(s) 713, 1976
BsrI ACTGG 7 cut(s) 189, 772, 1010, 1115, 1484, 2187, 2209
BssAI RCCGGY 2 cut(s) 713, 1976
BssECI CCNNGG 8 cut(s) 103, 151, 223, 453, 1201, 1561, 1649, 2328
BssMI GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 5 cut(s) 151, 223, 453, 1649, 2328
Bst2UI CCWGG 5 cut(s) 104, 118, 1202, 2289, 2556
Bst4CI ACNGT 9 cut(s) 124, 630, 640, 657, 760, 899, 1324, 1436, 1466
Bst6I CTCTTC 1 cut(s) 1386
BstACI GRCGYC 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 956
BstC8I GCNNGC 3 cut(s) 1330, 1500, 1698
BstDSI CCRYGG 1 cut(s) 2328
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 8 cut(s) 803, 1111, 1228, 1379, 1643, 2233, 2300, 2466
BstH2I RGCGCY 1 cut(s) 882
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 6 cut(s) 402, 847, 1072, 1756, 2052, 2233
BstMAI GTCTC 4 cut(s) 46, 233, 316, 2419
BstMBI GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
BstMWI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1139, 1241, 1670, 1946, 2333
BstNI CCWGG 5 cut(s) 104, 118, 1202, 2289, 2556
BstNSI RCATGY 5 cut(s) 132, 246, 253, 1700, 2135
BstPAI GACNNNNGTC 1 cut(s) 1563
BstSCI CCNGG 5 cut(s) 102, 116, 1200, 2287, 2554
BstSFI CTRYAG 4 cut(s) 1245, 1496, 1668, 1966
BstSLI GKGCMC 1 cut(s) 1279
BstV1I GCAGC 9 cut(s) 959, 1117, 1231, 1259, 1437, 1657, 1952, 2320, 2488
BstV2I GAAGAC 2 cut(s) 157, 1559
BstXI CCANNNNNNTGG 3 cut(s) 357, 1208, 1223
Bsu15I ATCGAT 1 cut(s) 1890
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 3 cut(s) 111, 624, 2139
BsuTUI ATCGAT 1 cut(s) 1890
BtgI CCRYGG 1 cut(s) 2328
BtrI CACGTC 2 cut(s) 1040, 2552
BtsCI GGATG 8 cut(s) 803, 1111, 1228, 1379, 1643, 2233, 2300, 2466
BtsI GCAGTG 4 cut(s) 18, 1493, 1590, 1806
Cac8I GCNNGC 3 cut(s) 1330, 1500, 1698
CfoI GCGC 1 cut(s) 881
Cfr10I RCCGGY 2 cut(s) 713, 1976
Cfr13I GGNCC 2 cut(s) 148, 623
ClaI ATCGAT 1 cut(s) 1890
Csp6I GTAC 7 cut(s) 180, 725, 895, 978, 1368, 2180, 2245
CviQI GTAC 7 cut(s) 180, 725, 895, 978, 1368, 2180, 2245
DinI GGCGCC 1 cut(s) 880
DpnI GATC 6 cut(s) 401, 846, 1071, 1755, 2051, 2232
DpnII GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
EaeI YGGCCR 2 cut(s) 109, 2137
Eam1104I CTCTTC 1 cut(s) 1386
EarI CTCTTC 1 cut(s) 1386
Eco130I CCWWGG 5 cut(s) 151, 223, 453, 1649, 2328
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 593, 2024, 2583
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 2376
EcoRII CCWGG 5 cut(s) 102, 116, 1200, 2287, 2554
EcoT14I CCWWGG 5 cut(s) 151, 223, 453, 1649, 2328
EcoT22I ATGCAT 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 880
EheI GGCGCC 1 cut(s) 880
ErhI CCWWGG 5 cut(s) 151, 223, 453, 1649, 2328
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 2402, 2434
FauNDI CATATG 2 cut(s) 1252, 1299
FbaI TGATCA 1 cut(s) 399
FblI GTMKAC 1 cut(s) 2299
FokI GGATG 8 cut(s) 810, 1098, 1235, 1386, 1650, 2240, 2307, 2453
FspBI CTAG 3 cut(s) 944, 1884, 1910
GlaI GCGC 1 cut(s) 880
GsuI CTGGAG 4 cut(s) 789, 1169, 1257, 2192
HaeII RGCGCY 1 cut(s) 882
HaeIII GGCC 3 cut(s) 111, 624, 2139
HapII CCGG 7 cut(s) 442, 714, 1371, 1977, 2047, 2225, 2470
HhaI GCGC 1 cut(s) 881
Hin1I GRCGYC 1 cut(s) 879
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 3 cut(s) 1043, 1104, 1410
HindII GTYRAC 3 cut(s) 1043, 1104, 1410
HindIII AAGCTT 3 cut(s) 316, 559, 1140
HinfI GANTC 7 cut(s) 470, 1604, 1887, 2106, 2174, 2346, 2451
HpaII CCGG 7 cut(s) 442, 714, 1371, 1977, 2047, 2225, 2470
HphI GGTGA 3 cut(s) 199, 370, 685
Hpy166II GTNNAC 8 cut(s) 182, 653, 895, 1043, 1104, 1410, 1462, 2300
Hpy188I TCNGA 8 cut(s) 487, 498, 705, 1023, 1031, 1758, 1993, 2089
Hpy188III TCNNGA 9 cut(s) 358, 371, 410, 442, 917, 1067, 1910, 2343, 2470
Hpy8I GTNNAC 8 cut(s) 182, 653, 895, 1043, 1104, 1410, 1462, 2300
HpyCH4III ACNGT 9 cut(s) 124, 630, 640, 657, 760, 899, 1324, 1436, 1466
HpyCH4IV ACGT 2 cut(s) 1039, 2551
HpyF10VI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1139, 1241, 1670, 1946, 2333
HpySE526I ACGT 2 cut(s) 1039, 2551
Hsp92I GRCGYC 1 cut(s) 879
HspAI GCGC 1 cut(s) 879
KasI GGCGCC 1 cut(s) 878
Kpn2I TCCGGA 2 cut(s) 441, 2469
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
LmnI GCTCC 3 cut(s) 1150, 1193, 2356
Lsp1109I GCAGC 9 cut(s) 959, 1117, 1231, 1259, 1437, 1657, 1952, 2320, 2488
LweI GCATC 4 cut(s) 788, 1142, 1989, 2020
MaeI CTAG 3 cut(s) 944, 1884, 1910
MaeII ACGT 2 cut(s) 1039, 2551
MaeIII GTNAC 8 cut(s) 124, 425, 471, 917, 1054, 1339, 2066, 2317
MalI GATC 6 cut(s) 401, 846, 1071, 1755, 2051, 2232
MboI GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
MboII GAAGA 7 cut(s) 157, 346, 679, 1403, 1559, 1838, 2331
MfeI CAATTG 1 cut(s) 2078
MhlI GDGCHC 2 cut(s) 1279, 2361
MlsI TGGCCA 1 cut(s) 111
MluNI TGGCCA 1 cut(s) 111
Mly113I GGCGCC 1 cut(s) 879
MlyI GAGTC 3 cut(s) 479, 2183, 2340
MmeI TCCRAC 2 cut(s) 666, 2384
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 803
MroI TCCGGA 2 cut(s) 441, 2469
MroXI GAANNNNTTC 4 cut(s) 78, 341, 492, 2110
MscI TGGCCA 1 cut(s) 111
MseI TTAA 7 cut(s) 387, 962, 1379, 1818, 1953, 2004, 2309
MslI CAYNNNNRTG 5 cut(s) 1221, 1749, 2097, 2219, 2398
Msp20I TGGCCA 1 cut(s) 111
MspI CCGG 7 cut(s) 442, 714, 1371, 1977, 2047, 2225, 2470
MspR9I CCNGG 5 cut(s) 104, 118, 1202, 2289, 2556
MunI CAATTG 1 cut(s) 2078
MvaI CCWGG 5 cut(s) 104, 118, 1202, 2289, 2556
MwoI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1139, 1241, 1670, 1946, 2333
NarI GGCGCC 1 cut(s) 879
NcoI CCATGG 1 cut(s) 2328
NdeI CATATG 2 cut(s) 1252, 1299
NdeII GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
NlaIV GGNNCC 2 cut(s) 880, 1933
NmuCI GTSAC 5 cut(s) 124, 471, 917, 1054, 2066
NsiI ATGCAT 1 cut(s) 803
NspI RCATGY 5 cut(s) 132, 246, 253, 1700, 2135
PaeI GCATGC 1 cut(s) 1700
PciI ACATGT 3 cut(s) 128, 249, 2131
PdmI GAANNNNTTC 4 cut(s) 78, 341, 492, 2110
PfeI GAWTC 4 cut(s) 1604, 1887, 2106, 2451
PflFI GACNNNGTC 1 cut(s) 437
PleI GAGTC 3 cut(s) 478, 2182, 2340
PluTI GGCGCC 1 cut(s) 882
PpsI GAGTC 3 cut(s) 478, 2182, 2340
PscI ACATGT 3 cut(s) 128, 249, 2131
PshAI GACNNNNGTC 1 cut(s) 1563
PsiI TTATAA 1 cut(s) 557
Psp6I CCWGG 5 cut(s) 102, 116, 1200, 2287, 2554
PspGI CCWGG 5 cut(s) 102, 116, 1200, 2287, 2554
PspN4I GGNNCC 2 cut(s) 880, 1933
PspPI GGNCC 2 cut(s) 148, 623
PsrI GAACNNNNNNTAC 2 cut(s) 855, 887
PstI CTGCAG 3 cut(s) 1249, 1500, 1970
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 7 cut(s) 181, 726, 896, 979, 1369, 2181, 2246
RsaNI GTAC 7 cut(s) 180, 725, 895, 978, 1368, 2180, 2245
RseI CAYNNNNRTG 5 cut(s) 1221, 1749, 2097, 2219, 2398
SaqAI TTAA 7 cut(s) 387, 962, 1379, 1818, 1953, 2004, 2309
Sau3AI GATC 6 cut(s) 399, 844, 1069, 1753, 2049, 2230
Sau96I GGNCC 2 cut(s) 148, 623
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 3 cut(s) 479, 2183, 2340
ScrFI CCNGG 5 cut(s) 104, 118, 1202, 2289, 2556
SduI GDGCHC 2 cut(s) 1279, 2361
SfaNI GCATC 4 cut(s) 788, 1142, 1989, 2020
SfcI CTRYAG 4 cut(s) 1245, 1496, 1668, 1966
SfoI GGCGCC 1 cut(s) 880
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 5 cut(s) 1221, 1749, 2097, 2219, 2398
SmlI CTYRAG 3 cut(s) 313, 410, 419
SmoI CTYRAG 3 cut(s) 313, 410, 419
SphI GCATGC 1 cut(s) 1700
SsiI CCGC 2 cut(s) 621, 2074
SspDI GGCGCC 1 cut(s) 878
SspI AATATT 3 cut(s) 837, 1815, 2483
SspMI CTAG 3 cut(s) 944, 1884, 1910
StyD4I CCNGG 5 cut(s) 102, 116, 1200, 2287, 2554
StyI CCWWGG 5 cut(s) 151, 223, 453, 1649, 2328
TaaI ACNGT 9 cut(s) 124, 630, 640, 657, 760, 899, 1324, 1436, 1466
TaiI ACGT 2 cut(s) 1042, 2554
TaqI TCGA 4 cut(s) 301, 1136, 1729, 1890
TaqII GACCGA 1 cut(s) 1575
TatI WGTACW 5 cut(s) 179, 894, 977, 2179, 2244
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 4 cut(s) 1604, 1887, 2106, 2451
Tru1I TTAA 7 cut(s) 387, 962, 1379, 1818, 1953, 2004, 2309
Tru9I TTAA 7 cut(s) 387, 962, 1379, 1818, 1953, 2004, 2309
TseFI GTSAC 5 cut(s) 124, 471, 917, 1054, 2066
TseI GCWGC 9 cut(s) 947, 1130, 1244, 1247, 1425, 1645, 1965, 2333, 2501
Tsp45I GTSAC 5 cut(s) 124, 471, 917, 1054, 2066
TspGWI ACGGA 2 cut(s) 1434, 1438
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 8 cut(s) 786, 1179, 2006, 2144, 2254, 2376, 2413, 2461
XbaI TCTAGA 1 cut(s) 1909
XceI RCATGY 5 cut(s) 132, 246, 253, 1700, 2135
XmiI GTMKAC 1 cut(s) 2299
XmnI GAANNNNTTC 4 cut(s) 78, 341, 492, 2110
XspI CTAG 3 cut(s) 944, 1884, 1910
ZrmI AGTACT 1 cut(s) 979
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.