Rh4CG060100

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000555
Physical Location & Seq
Reverse (-)
11473768 .. 11490001
16234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG060100.1

Sequence Viewer

Length: 696 bp
ATGAGCTTATTAGAAGAGGGAGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACTGCAGGCTGATTTAGGCATTGCAGTTGCATATATCAGTGGCGATTTTTCTTTCAATGATAAGGACCGAGGTCTTCTAAATCTTTTTATGGCTAGAATCGATGAGAACCATGAGGTTCTAGATATTGGTTGTGGTTGGGGAACCTTAGCTATTGAAGCAGTCAAACAAACAGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATTTCTTGCGAGATGTTAGAACATGTCGGCCATGAATTTATGGATGAGTTCTTTTCTTGCTGTGAGTCTGTACTGGCAAAAAATGGGCTTTTTATTCTCCAGTTCATATCTGTGCCGGATGATCGGTATGATGAGTACAGGCAAAATTCAGGCTACTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACCTTCATTAAGTAGGGTAACATCAGCCATGGCCGCTTCTTCTGGACTCTGGTATGATCTGTTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

231

Amino Acids

26.26

Weight (kDa)

5.27

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTS PF05175 52 - 176 5.9e-10 Methyltransferase small domain
Methyltransf_23 PF13489 64 - 197 1.4e-12 Methyltransferase domain
Methyltransf_18 PF12847 69 - 179 2.5e-06 Methyltransferase domain
CMAS PF02353 70 - 220 2.4e-42 Mycolic acid cyclopropane synthetase
PrmA PF06325 70 - 178 3.7e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 73 - 182 8e-16 Methyltransferase domain
Methyltransf_25 PF13649 78 - 174 3.7e-19 Methyltransferase domain
Methyltransf_11 PF08241 79 - 178 3.6e-16 Methyltransferase domain
Methyltransf_12 PF08242 79 - 176 1.2e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 625
AciI CCGC 2 cut(s) 400, 660
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 2 cut(s) 463, 657
AcsI RAATTY 3 cut(s) 332, 470, 580
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 2 cut(s) 507, 572
AflIII ACRYGT 1 cut(s) 457
AgsI TTSAA 2 cut(s) 172, 272
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 2 cut(s) 6, 266
AluI AGCT 2 cut(s) 6, 266
AlwI GGATC 1 cut(s) 383
AoxI GGCC 2 cut(s) 463, 657
ApeKI GCWGC 1 cut(s) 291
ApoI RAATTY 3 cut(s) 332, 470, 580
Asp700I GAANNNNTTC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 615
BfaI CTAG 2 cut(s) 210, 236
BfmI CTRYAG 2 cut(s) 119, 292
BisI GCNGC 2 cut(s) 292, 660
BlsI GCNGC 2 cut(s) 293, 661
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 2 cut(s) 315, 346
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 184, 654
BsaWI WCCGGW 1 cut(s) 372
Bse1I ACTGG 4 cut(s) 107, 307, 513, 535
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 615
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 184, 654
BseGI GGATG 3 cut(s) 484, 559, 626
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 2 cut(s) 107, 309
BseNI ACTGG 4 cut(s) 107, 307, 513, 535
BseXI GCAGC 1 cut(s) 278
BshFI GGCC 2 cut(s) 465, 659
BshVI ATCGAT 1 cut(s) 216
BsiSI CCGG 2 cut(s) 373, 551
BsnI GGCC 2 cut(s) 465, 659
Bsp143I GATC 3 cut(s) 375, 556, 682
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 2 cut(s) 400, 660
BspANI GGCC 2 cut(s) 465, 659
BspCNI CTCAG 2 cut(s) 106, 310
BspDI ATCGAT 1 cut(s) 216
BspHI TCATGA 1 cut(s) 692
BspLI GGNNCC 1 cut(s) 259
BspMAI CTGCAG 2 cut(s) 123, 296
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 4 cut(s) 107, 307, 513, 535
BssECI CCNNGG 2 cut(s) 184, 654
BssMI GATC 3 cut(s) 375, 556, 682
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 59, 89
Bst6I CTCTTC 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 3 cut(s) 93, 262, 318
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 3 cut(s) 484, 559, 626
BstKTI GATC 3 cut(s) 378, 559, 685
BstMBI GATC 3 cut(s) 375, 556, 682
BstMWI GCNNNNNNNGC 2 cut(s) 272, 659
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 1 cut(s) 461
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 2 cut(s) 119, 292
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 182
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 2 cut(s) 465, 659
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 3 cut(s) 484, 559, 626
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 3 cut(s) 116, 160, 300
Cac8I GCNNGC 1 cut(s) 123
CciI TCATGA 1 cut(s) 692
Cfr13I GGNCC 1 cut(s) 181
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 2 cut(s) 506, 571
CviAII CATG 5 cut(s) 227, 458, 467, 655, 693
CviQI GTAC 2 cut(s) 506, 571
DdeI CTNAG 3 cut(s) 93, 262, 318
DpnI GATC 3 cut(s) 377, 558, 684
DpnII GATC 3 cut(s) 375, 556, 682
EaeI YGGCCR 2 cut(s) 463, 657
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 5 cut(s) 230, 461, 470, 658, 696
FatI CATG 5 cut(s) 226, 457, 466, 654, 692
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 292, 660
FokI GGATG 3 cut(s) 491, 566, 633
Fsp4HI GCNGC 2 cut(s) 292, 660
FspBI CTAG 2 cut(s) 210, 236
GluI GCNGC 2 cut(s) 292, 660
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 465, 659
HapII CCGG 2 cut(s) 373, 551
Hin1II CATG 5 cut(s) 230, 461, 470, 658, 696
HinfI GANTC 4 cut(s) 213, 432, 500, 672
HpaII CCGG 2 cut(s) 373, 551
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 3 cut(s) 33, 85, 626
Hpy188I TCNGA 2 cut(s) 319, 415
Hpy188III TCNNGA 3 cut(s) 236, 669, 693
Hpy8I GTNNAC 3 cut(s) 33, 85, 626
HpyAV CCTTC 3 cut(s) 395, 420, 639
HpyCH4III ACNGT 2 cut(s) 59, 89
HpyCH4V TGCA 4 cut(s) 121, 140, 146, 294
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 659
HpyF3I CTNAG 3 cut(s) 93, 262, 318
Hsp92II CATG 5 cut(s) 230, 461, 470, 658, 696
Kzo9I GATC 3 cut(s) 375, 556, 682
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 2 cut(s) 315, 346
MaeI CTAG 2 cut(s) 210, 236
MaeIII GTNAC 2 cut(s) 392, 643
MalI GATC 3 cut(s) 377, 558, 684
MboI GATC 3 cut(s) 375, 556, 682
MboII GAAGA 3 cut(s) 26, 182, 657
MfeI CAATTG 1 cut(s) 404
MluCI AATT 4 cut(s) 332, 404, 470, 580
MlyI GAGTC 2 cut(s) 509, 666
MnlI CCTC 7 cut(s) 10, 14, 34, 102, 179, 223, 313
MroXI GAANNNNTTC 1 cut(s) 436
MseI TTAA 2 cut(s) 330, 635
MslI CAYNNNNRTG 2 cut(s) 423, 545
MspI CCGG 2 cut(s) 373, 551
MspR9I CCNGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 404
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 2 cut(s) 272, 659
NcoI CCATGG 1 cut(s) 654
NdeII GATC 3 cut(s) 375, 556, 682
NlaIII CATG 5 cut(s) 230, 461, 470, 658, 696
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 1 cut(s) 461
PagI TCATGA 1 cut(s) 692
PciI ACATGT 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 436
PfeI GAWTC 2 cut(s) 213, 432
PkrI GCNGC 2 cut(s) 293, 661
PleI GAGTC 2 cut(s) 508, 666
PpsI GAGTC 2 cut(s) 508, 666
PscI ACATGT 1 cut(s) 457
PshAI GACNNNNGTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 2 cut(s) 123, 296
RsaI GTAC 2 cut(s) 507, 572
RsaNI GTAC 2 cut(s) 506, 571
RseI CAYNNNNRTG 2 cut(s) 423, 545
SaqAI TTAA 2 cut(s) 330, 635
SatI GCNGC 2 cut(s) 292, 660
Sau3AI GATC 3 cut(s) 375, 556, 682
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 2 cut(s) 509, 666
ScrFI CCNGG 1 cut(s) 615
SetI ASST 7 cut(s) 8, 38, 190, 234, 263, 268, 631
SfaNI GCATC 2 cut(s) 315, 346
SfcI CTRYAG 2 cut(s) 119, 292
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 2 cut(s) 423, 545
Sse9I AATT 4 cut(s) 332, 404, 470, 580
SsiI CCGC 2 cut(s) 400, 660
SspMI CTAG 2 cut(s) 210, 236
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 2 cut(s) 59, 89
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 198
TasI AATT 4 cut(s) 332, 404, 470, 580
TatI WGTACW 2 cut(s) 505, 570
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 2 cut(s) 213, 432
Tru1I TTAA 2 cut(s) 330, 635
Tru9I TTAA 2 cut(s) 330, 635
TscAI CASTG 3 cut(s) 123, 160, 307
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 4 cut(s) 483, 529, 583, 621
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 3 cut(s) 123, 160, 307
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 3 cut(s) 332, 470, 580
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 436
XspI CTAG 2 cut(s) 210, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.