FvH4_4g04360

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000555
Physical Location & Seq
Reverse (-)
3743929 .. 3749475
5547 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04360.t8

Sequence Viewer

Length: 1218 bp
ATGGCTGCAGCAGATGGTATGCTTGGAAATGGTTGTGCGCTGTTGGGAAACACAAAACATATGGCTCTTTCATTAACCGAGACAGCAGCACGCCAGTTTGTTACTAGATTTTTTAGACATTTTATATCTGTTGGGTGTTTAATTTTATTAGAAGAAGGAGGCACAATGTTTACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAGATCCATTCTTAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACAGGCTGATTTAGGCATTGCAGATGCATATATCAATGGCGATTTTTCTTTCGATGATAAAGACCGAGATCTTTTTAATCTTTTTATGCTTCTCATTGCCAACGTAGAATCAAATGCCTCAAAATCAAACAAGAAAAGGCGATGGTGGCAACCGTTGTTATTCACAGCCACTATAGCCACAGCCAAATATTGTTTTCAGCATTTTTTAAGGCAAAATAGTCTTACACAGGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCACTGTTCTTGGGCGAAACAATGATGTATTCCACAGCAATATTTAAGACAGAAGATGAGGAACTGAAGACTGCACAGCTTAGGAAAATCTCTATTCTAATTGAAAAGGCTAGAATCGATGAGAAACATGAGGTTCTAGATATTGGTTGTGGTTGGGGAACCTTTGCTATTGAAGTAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGACGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGGAGCGGATCAACTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATATCTTGTGAGATGATAGAACACGTTGGCCATGAATTTGTGGATGAGTTCTTTTCCTGCTGTGAGTCTGTACTGGAAGAAAATGGGCTTTTTATTTTGCAGTTTATATCAATGCCGGATGACCGCTATGATGACTACAGGCGAGGTACAGGCTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACCTTCGTTAGGTAGGGTAATATCAGCCATGGCCGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGACTTCTGATTACTACTGTCTTTCTAACAGATTGCATGCCCTATCAGAAAAAAAAGGTGCATCCATTAGTTTTGAATGGAACAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

406

Amino Acids

46.26

Weight (kDa)

6.16

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 149 - 417 1.6e-80 Mycolic acid cyclopropane synthetase
MTS PF05175 199 - 311 5.9e-08 Methyltransferase small domain
Methyltransf_23 PF13489 204 - 315 4.5e-10 Methyltransferase domain
Methyltransf_31 PF13847 207 - 316 5.1e-13 Methyltransferase domain
Methyltransf_25 PF13649 212 - 308 9.6e-18 Methyltransferase domain
Methyltransf_11 PF08241 213 - 312 4.1e-15 Methyltransferase domain
Methyltransf_12 PF08242 213 - 310 8.4e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 805
AccI GTMKAC 1 cut(s) 1054
AciI CCGC 4 cut(s) 805, 832, 991, 1089
AclWI GGATC 2 cut(s) 201, 815
AcoI YGGCCR 2 cut(s) 895, 1086
AcsI RAATTY 2 cut(s) 764, 902
AcuI CTGAAG 2 cut(s) 617, 782
AfaI GTAC 2 cut(s) 939, 1015
AfiI CCNNNNNNNGG 1 cut(s) 1064
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 3 cut(s) 635, 704, 1204
AjnI CCWGG 1 cut(s) 1042
AluBI AGCT 1 cut(s) 610
AluI AGCT 1 cut(s) 610
Alw21I GWGCWC 1 cut(s) 1116
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 2 cut(s) 201, 815
AoxI GGCC 2 cut(s) 895, 1086
ApeKI GCWGC 3 cut(s) 5, 8, 86
ApoI RAATTY 2 cut(s) 764, 902
AspLEI GCGC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 897
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 1116
BbvI GCAGC 2 cut(s) 20, 98
BccI CCATC 2 cut(s) 8, 390
BciT130I CCWGG 1 cut(s) 1044
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 4 cut(s) 105, 642, 668, 1098
BfmI CTRYAG 3 cut(s) 6, 426, 1003
BglII AGATCT 1 cut(s) 322
BisI GCNGC 4 cut(s) 6, 9, 87, 1089
BlsI GCNGC 4 cut(s) 7, 10, 88, 1090
Bme1390I CCNGG 1 cut(s) 1044
BmiI GGNNCC 1 cut(s) 691
BmrFI CCNGG 1 cut(s) 1044
BmsI GCATC 4 cut(s) 268, 747, 778, 1198
BpiI GAAGAC 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 648
BsaJI CCNNGG 1 cut(s) 1083
Bsc4I CCNNNNNNNGG 1 cut(s) 1064
Bse1I ACTGG 4 cut(s) 94, 245, 739, 945
Bse3DI GCAATG 2 cut(s) 270, 348
BseBI CCWGG 1 cut(s) 1044
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 1 cut(s) 1083
BseGI GGATG 4 cut(s) 916, 991, 1055, 1189
BseLI CCNNNNNNNGG 1 cut(s) 1064
BseMI GCAATG 2 cut(s) 270, 348
BseMII CTCAG 2 cut(s) 245, 506
BseNI ACTGG 4 cut(s) 94, 245, 739, 945
BseXI GCAGC 2 cut(s) 20, 98
BsgI GTGCAG 1 cut(s) 588
BshFI GGCC 2 cut(s) 897, 1088
BshVI ATCGAT 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 1116
BsiSI CCGG 1 cut(s) 983
BslI CCNNNNNNNGG 1 cut(s) 1064
BsmAI GTCTC 1 cut(s) 74
BsnI GGCC 2 cut(s) 897, 1088
Bsp1286I GDGCHC 1 cut(s) 1116
Bsp143I GATC 4 cut(s) 206, 322, 511, 807
Bsp19I CCATGG 1 cut(s) 1083
BspACI CCGC 4 cut(s) 805, 832, 991, 1089
BspANI GGCC 2 cut(s) 897, 1088
BspCNI CTCAG 2 cut(s) 244, 507
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 691
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 201, 815
BsrBI CCGCTC 1 cut(s) 805
BsrDI GCAATG 2 cut(s) 270, 348
BsrI ACTGG 4 cut(s) 94, 245, 739, 945
BssECI CCNNGG 1 cut(s) 1083
BssMI GATC 4 cut(s) 206, 322, 511, 807
BssT1I CCWWGG 1 cut(s) 1083
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 5 cut(s) 197, 227, 408, 537, 1147
BstC8I GCNNGC 2 cut(s) 91, 1166
BstDEI CTNAG 4 cut(s) 203, 231, 515, 611
BstDSI CCRYGG 1 cut(s) 1083
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 4 cut(s) 916, 991, 1055, 1189
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 209, 325, 514, 810
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 4 cut(s) 206, 322, 511, 807
BstMWI GCNNNNNNNGC 3 cut(s) 400, 428, 1088
BstNI CCWGG 1 cut(s) 1044
BstNSI RCATGY 1 cut(s) 1168
BstSCI CCNGG 1 cut(s) 1042
BstSFI CTRYAG 3 cut(s) 6, 426, 1003
BstV1I GCAGC 2 cut(s) 20, 98
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 206, 322
BstYI RGATCY 2 cut(s) 206, 322
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 2 cut(s) 897, 1088
BsuTUI ATCGAT 1 cut(s) 648
BtgI CCRYGG 1 cut(s) 1083
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 4 cut(s) 916, 991, 1055, 1189
BtsIMutI CAGTG 2 cut(s) 533, 732
Cac8I GCNNGC 2 cut(s) 91, 1166
CfoI GCGC 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 2 cut(s) 938, 1014
CviAII CATG 4 cut(s) 659, 899, 1084, 1165
CviQI GTAC 2 cut(s) 938, 1014
DdeI CTNAG 4 cut(s) 203, 231, 515, 611
DpnI GATC 4 cut(s) 208, 324, 513, 809
DpnII GATC 4 cut(s) 206, 322, 511, 807
EaeI YGGCCR 2 cut(s) 895, 1086
Eco130I CCWWGG 1 cut(s) 1083
Eco57I CTGAAG 2 cut(s) 617, 782
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoRII CCWGG 1 cut(s) 1042
EcoT14I CCWWGG 1 cut(s) 1083
EcoT22I ATGCAT 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 1083
FaeI CATG 4 cut(s) 662, 902, 1087, 1168
FatI CATG 4 cut(s) 658, 898, 1083, 1164
FauNDI CATATG 1 cut(s) 60
FblI GTMKAC 1 cut(s) 1054
Fnu4HI GCNGC 4 cut(s) 6, 9, 87, 1089
FokI GGATG 4 cut(s) 923, 998, 1062, 1176
Fsp4HI GCNGC 4 cut(s) 6, 9, 87, 1089
FspBI CTAG 4 cut(s) 105, 642, 668, 1098
GlaI GCGC 1 cut(s) 39
GluI GCNGC 4 cut(s) 6, 9, 87, 1089
HaeIII GGCC 2 cut(s) 897, 1088
HapII CCGG 1 cut(s) 983
HhaI GCGC 1 cut(s) 40
Hin1II CATG 4 cut(s) 662, 902, 1087, 1168
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 5 cut(s) 362, 645, 864, 932, 1101
HpaII CCGG 1 cut(s) 983
Hpy166II GTNNAC 3 cut(s) 171, 223, 1055
Hpy188I TCNGA 5 cut(s) 516, 751, 847, 1137, 1176
Hpy188III TCNNGA 3 cut(s) 668, 800, 1098
Hpy8I GTNNAC 3 cut(s) 171, 223, 1055
HpyAV CCTTC 5 cut(s) 149, 483, 827, 852, 1068
HpyCH4III ACNGT 5 cut(s) 197, 227, 408, 537, 1147
HpyCH4IV ACGT 2 cut(s) 357, 891
HpyCH4V TGCA 8 cut(s) 8, 275, 281, 605, 726, 967, 1164, 1189
HpyF10VI GCNNNNNNNGC 3 cut(s) 400, 428, 1088
HpyF3I CTNAG 4 cut(s) 203, 231, 515, 611
HpySE526I ACGT 2 cut(s) 357, 891
Hsp92II CATG 4 cut(s) 662, 902, 1087, 1168
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 4 cut(s) 206, 322, 511, 807
LmnI GCTCC 2 cut(s) 802, 1111
Lsp1109I GCAGC 2 cut(s) 20, 98
LweI GCATC 4 cut(s) 268, 747, 778, 1198
MaeI CTAG 4 cut(s) 105, 642, 668, 1098
MaeII ACGT 2 cut(s) 357, 891
MaeIII GTNAC 2 cut(s) 100, 824
MalI GATC 4 cut(s) 208, 324, 513, 809
MbiI CCGCTC 1 cut(s) 805
MboI GATC 4 cut(s) 206, 322, 511, 807
MboII GAAGA 5 cut(s) 164, 596, 610, 956, 1086
MfeI CAATTG 1 cut(s) 836
MflI RGATCY 2 cut(s) 206, 322
MhlI GDGCHC 1 cut(s) 1116
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 5 cut(s) 141, 630, 764, 836, 902
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 2 cut(s) 941, 1095
MnlI CCTC 7 cut(s) 152, 172, 240, 382, 583, 655, 1004
Mox20I TGGCCA 1 cut(s) 897
Mph1103I ATGCAT 1 cut(s) 283
MscI TGGCCA 1 cut(s) 897
MseI TTAA 7 cut(s) 74, 140, 216, 330, 461, 576, 762
MslI CAYNNNNRTG 1 cut(s) 855
Msp20I TGGCCA 1 cut(s) 897
MspI CCGG 1 cut(s) 983
MspR9I CCNGG 1 cut(s) 1044
MunI CAATTG 1 cut(s) 836
MvaI CCWGG 1 cut(s) 1044
MwoI GCNNNNNNNGC 3 cut(s) 400, 428, 1088
NcoI CCATGG 1 cut(s) 1083
NdeI CATATG 1 cut(s) 60
NdeII GATC 4 cut(s) 206, 322, 511, 807
NlaIII CATG 4 cut(s) 662, 902, 1087, 1168
NlaIV GGNNCC 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 824
NsiI ATGCAT 1 cut(s) 283
NspI RCATGY 1 cut(s) 1168
PaeI GCATGC 1 cut(s) 1168
PfeI GAWTC 3 cut(s) 362, 645, 864
PkrI GCNGC 4 cut(s) 7, 10, 88, 1090
PleI GAGTC 2 cut(s) 940, 1095
PpsI GAGTC 2 cut(s) 940, 1095
Psp6I CCWGG 1 cut(s) 1042
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 1 cut(s) 691
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 206, 322
RsaI GTAC 2 cut(s) 939, 1015
RsaNI GTAC 2 cut(s) 938, 1014
RseI CAYNNNNRTG 1 cut(s) 855
SaqAI TTAA 7 cut(s) 74, 140, 216, 330, 461, 576, 762
SatI GCNGC 4 cut(s) 6, 9, 87, 1089
Sau3AI GATC 4 cut(s) 206, 322, 511, 807
SchI GAGTC 2 cut(s) 941, 1095
ScrFI CCNGG 1 cut(s) 1044
SduI GDGCHC 1 cut(s) 1116
SfaNI GCATC 4 cut(s) 268, 747, 778, 1198
SfcI CTRYAG 3 cut(s) 6, 426, 1003
SmiMI CAYNNNNRTG 1 cut(s) 855
SphI GCATGC 1 cut(s) 1168
Sse9I AATT 5 cut(s) 141, 630, 764, 836, 902
SsiI CCGC 4 cut(s) 805, 832, 991, 1089
SspI AATATT 2 cut(s) 443, 573
SspMI CTAG 4 cut(s) 105, 642, 668, 1098
StyD4I CCNGG 1 cut(s) 1042
StyI CCWWGG 1 cut(s) 1083
TaaI ACNGT 5 cut(s) 197, 227, 408, 537, 1147
TaiI ACGT 2 cut(s) 360, 894
TaqI TCGA 3 cut(s) 306, 487, 648
TaqII GACCGA 1 cut(s) 333
TasI AATT 5 cut(s) 141, 630, 764, 836, 902
TatI WGTACW 1 cut(s) 937
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 3 cut(s) 362, 645, 864
Tru1I TTAA 7 cut(s) 74, 140, 216, 330, 461, 576, 762
Tru9I TTAA 7 cut(s) 74, 140, 216, 330, 461, 576, 762
TscAI CASTG 2 cut(s) 540, 739
TseFI GTSAC 1 cut(s) 824
TseI GCWGC 3 cut(s) 5, 8, 86
Tsp45I GTSAC 1 cut(s) 824
TspDTI ATGAA 4 cut(s) 60, 537, 915, 1012
TspRI CASTG 2 cut(s) 540, 739
XagI CCTNNNNNAGG 1 cut(s) 1062
XapI RAATTY 2 cut(s) 764, 902
XbaI TCTAGA 2 cut(s) 667, 1097
XceI RCATGY 1 cut(s) 1168
XmiI GTMKAC 1 cut(s) 1054
XspI CTAG 4 cut(s) 105, 642, 668, 1098
Zsp2I ATGCAT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.