Rw6G016840

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000555
Physical Location & Seq
Reverse (-)
33224490 .. 33235316
10827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016840.1

Sequence Viewer

Length: 1284 bp
ATGGCTCTTTCATTGACCGAAACAGTGGCACGCTTATTTGTTACTAGATTTCTTAGAAATTATATATCTTCTGGATGTTTAATCTTATTAGAAGAGGGAGGCACAGTGTTCACTTTTGAGGGAAGCAAAAAGGATTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCACTTCTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATTAGTGGAGACTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTCATTGCCAACGGAGATTTTAATGCCTCAAAATCAAACAAGAAAAGGAGATGGTGGCAGCCTGTGTTTTTCACGGCTACTATAGCCACAGCCAAATATGTCTTCCAGCATGCTTTAAGGAAAAATAGTCTTACACAAGCTCGAAGGAACATATCTCGTCATTATGATTTGAGTAATGATCTATTTTCTCTGTTCTTGGGTGAAACAATGATGTATTCCACTGCAATATTTAAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTCTACTCTCATTGATAAGGCTACAATCGATGAGAACCATGAGGTTCTAGAAATTGGTTGTGGTTGGGGAGCCTTAGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAAGAGCAACTGAAATTTGCTGAAAAAAAAGTGAAAGATGCTGGACTTCAGGACCGGATCAAGTTTCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATAATTTGCGAGGTGCTAGAACATGTCGGCCATGAATTTGTGGATGAGTTTTTTTCTTGCTGTGACTCTTTACTAGCAGAAAATGGGATTCTTGTTCTGCAGTTCATATGTATACTGGATGAACTCTATGATGAGTATCGGCGAAGTCCAGGCTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACTTTCATTAAGTAGGGTAACATCAGCCATGGCTGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGACATCATCATTATTACCAAACACTAAGATGTTGGAGAAAGAATTTTTTAGAGAGACAGAGCGAAATACTCGCTCTTGGATTCAATGAAAATTTCATTCGGACATGGGAATATTATTTTGATTATTGTGCTGCTGGTTTCAAGTCATATACACTTAGAGATTGCCAGATTGTGTTTTCACGTCCTGGTAATCCTCTCACGTTCAACAATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

427

Amino Acids

49.17

Weight (kDa)

6.33

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 139 - 409 6.8e-76 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 186 - 306 1.3e-10 Methyltransferase domain
Methyltransf_31 PF13847 197 - 319 6.7e-14 Methyltransferase domain
MTS PF05175 197 - 301 1.1e-06 Methyltransferase small domain
Methyltransf_25 PF13649 202 - 298 8.8e-17 Methyltransferase domain
Methyltransf_11 PF08241 203 - 302 1.8e-14 Methyltransferase domain
Methyltransf_12 PF08242 203 - 300 5.3e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 919, 994
AciI CCGC 1 cut(s) 772
AclWI GGATC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 835
AcsI RAATTY 4 cut(s) 704, 842, 1111, 1159
AcuI CTGAAG 2 cut(s) 711, 722
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 4 cut(s) 644, 1153, 1210, 1273
AjiI CACGTC 1 cut(s) 1250
AjnI CCWGG 3 cut(s) 955, 982, 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 3 cut(s) 425, 550, 638
AluI AGCT 3 cut(s) 425, 550, 638
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 2 cut(s) 228, 1117
AlwI GGATC 1 cut(s) 755
AoxI GGCC 1 cut(s) 835
ApeKI GCWGC 3 cut(s) 343, 1028, 1199
ApoI RAATTY 4 cut(s) 704, 842, 1111, 1159
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspS9I GGNCC 1 cut(s) 742
AsuHPI GGTGA 1 cut(s) 497
AvaII GGWCC 1 cut(s) 742
BbsI GAAGAC 2 cut(s) 257, 379
Bbv12I GWGCWC 1 cut(s) 1056
BbvI GCAGC 3 cut(s) 355, 1015, 1186
BccI CCATC 1 cut(s) 330
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 3 cut(s) 957, 984, 1254
BcoDI GTCTC 2 cut(s) 228, 1117
BfaI CTAG 6 cut(s) 45, 206, 608, 824, 881, 1038
BfmI CTRYAG 2 cut(s) 366, 905
BisI GCNGC 3 cut(s) 344, 1029, 1200
BlsI GCNGC 3 cut(s) 345, 1030, 1201
Bme1390I CCNGG 3 cut(s) 957, 984, 1254
Bme18I GGWCC 1 cut(s) 742
BmgBI CACGTC 1 cut(s) 1250
BmgT120I GGNCC 1 cut(s) 742
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 3 cut(s) 957, 984, 1254
BmsI GCATC 3 cut(s) 208, 687, 718
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 2 cut(s) 257, 379
Bpu10I CCTNAGC 2 cut(s) 551, 634
Bsa29I ATCGAT 1 cut(s) 588
BsaBI GATNNNNATC 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 259, 1023
BsaWI WCCGGW 1 cut(s) 744
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse1I ACTGG 3 cut(s) 185, 679, 927
Bse3DI GCAATG 2 cut(s) 210, 288
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 3 cut(s) 957, 984, 1254
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 2 cut(s) 259, 1023
BseGI GGATG 4 cut(s) 80, 856, 931, 995
BseJI GATNNNNATC 1 cut(s) 942
BseMI GCAATG 2 cut(s) 210, 288
BseNI ACTGG 3 cut(s) 185, 679, 927
BseXI GCAGC 3 cut(s) 355, 1015, 1186
BsgI GTGCAG 1 cut(s) 528
BshFI GGCC 1 cut(s) 837
BshVI ATCGAT 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 1056
BsiSI CCGG 1 cut(s) 745
BsmAI GTCTC 2 cut(s) 228, 1117
BsnI GGCC 1 cut(s) 837
Bsp1286I GDGCHC 1 cut(s) 1056
Bsp143I GATC 3 cut(s) 202, 463, 747
Bsp19I CCATGG 1 cut(s) 1023
BspACI CCGC 1 cut(s) 772
BspANI GGCC 1 cut(s) 837
BspDI ATCGAT 1 cut(s) 588
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 909
BspPI GGATC 1 cut(s) 755
BspQI GCTCTTC 1 cut(s) 687
BsrDI GCAATG 2 cut(s) 210, 288
BsrI ACTGG 3 cut(s) 185, 679, 927
BssECI CCNNGG 2 cut(s) 259, 1023
BssMI GATC 3 cut(s) 202, 463, 747
BssNAI GTATAC 1 cut(s) 920
BssT1I CCWWGG 1 cut(s) 1023
Bst1107I GTATAC 1 cut(s) 920
Bst2UI CCWGG 3 cut(s) 957, 984, 1254
Bst4CI ACNGT 3 cut(s) 25, 106, 167
Bst6I CTCTTC 2 cut(s) 87, 687
BstC8I GCNNGC 2 cut(s) 31, 396
BstDEI CTNAG 5 cut(s) 53, 551, 634, 1094, 1223
BstDSI CCRYGG 1 cut(s) 1023
BstF5I GGATG 4 cut(s) 80, 856, 931, 995
BstKTI GATC 3 cut(s) 205, 466, 750
BstMAI GTCTC 2 cut(s) 228, 1117
BstMBI GATC 3 cut(s) 202, 463, 747
BstMWI GCNNNNNNNGC 3 cut(s) 368, 644, 1028
BstNI CCWGG 3 cut(s) 957, 984, 1254
BstNSI RCATGY 2 cut(s) 398, 833
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 3 cut(s) 955, 982, 1252
BstSFI CTRYAG 2 cut(s) 366, 905
BstV1I GCAGC 3 cut(s) 355, 1015, 1186
BstV2I GAAGAC 2 cut(s) 257, 379
BstZ17I GTATAC 1 cut(s) 920
Bsu15I ATCGAT 1 cut(s) 588
BsuRI GGCC 1 cut(s) 837
BsuTUI ATCGAT 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 1023
BtrI CACGTC 1 cut(s) 1250
BtsCI GGATG 4 cut(s) 80, 856, 931, 995
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 4 cut(s) 30, 111, 504, 672
Cac8I GCNNGC 2 cut(s) 31, 396
Cfr13I GGNCC 1 cut(s) 742
ClaI ATCGAT 1 cut(s) 588
CviAII CATG 6 cut(s) 395, 599, 830, 839, 1024, 1173
DdeI CTNAG 5 cut(s) 53, 551, 634, 1094, 1223
DpnI GATC 3 cut(s) 204, 465, 749
DpnII GATC 3 cut(s) 202, 463, 747
EaeI YGGCCR 1 cut(s) 835
Eam1104I CTCTTC 2 cut(s) 87, 687
EarI CTCTTC 2 cut(s) 87, 687
Eco130I CCWWGG 1 cut(s) 1023
Eco47I GGWCC 1 cut(s) 742
Eco57I CTGAAG 2 cut(s) 711, 722
EcoRII CCWGG 3 cut(s) 955, 982, 1252
EcoT14I CCWWGG 1 cut(s) 1023
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 1023
FaeI CATG 6 cut(s) 398, 602, 833, 842, 1027, 1176
FatI CATG 6 cut(s) 394, 598, 829, 838, 1023, 1172
FauNDI CATATG 1 cut(s) 914
FblI GTMKAC 2 cut(s) 919, 994
Fnu4HI GCNGC 3 cut(s) 344, 1029, 1200
FokI GGATG 4 cut(s) 87, 863, 938, 1002
Fsp4HI GCNGC 3 cut(s) 344, 1029, 1200
FspBI CTAG 6 cut(s) 45, 206, 608, 824, 881, 1038
GluI GCNGC 3 cut(s) 344, 1029, 1200
HaeIII GGCC 1 cut(s) 837
HapII CCGG 1 cut(s) 745
Hin1II CATG 6 cut(s) 398, 602, 833, 842, 1027, 1176
HinfI GANTC 5 cut(s) 804, 872, 895, 1041, 1149
HpaII CCGG 1 cut(s) 745
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 111, 163, 920, 995
Hpy188I TCNGA 3 cut(s) 691, 787, 1170
Hpy188III TCNNGA 4 cut(s) 72, 608, 740, 1038
Hpy8I GTNNAC 4 cut(s) 111, 163, 920, 995
HpyAV CCTTC 2 cut(s) 423, 792
HpyCH4III ACNGT 3 cut(s) 25, 106, 167
HpyCH4IV ACGT 2 cut(s) 1249, 1268
HpyCH4V TGCA 6 cut(s) 215, 221, 509, 545, 666, 907
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 644, 1028
HpyF3I CTNAG 5 cut(s) 53, 551, 634, 1094, 1223
HpySE526I ACGT 2 cut(s) 1249, 1268
Hsp92II CATG 6 cut(s) 398, 602, 833, 842, 1027, 1176
Kzo9I GATC 3 cut(s) 202, 463, 747
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 2 cut(s) 629, 1051
Lsp1109I GCAGC 3 cut(s) 355, 1015, 1186
LweI GCATC 3 cut(s) 208, 687, 718
MaeI CTAG 6 cut(s) 45, 206, 608, 824, 881, 1038
MaeII ACGT 2 cut(s) 1249, 1268
MaeIII GTNAC 4 cut(s) 40, 764, 869, 1012
MalI GATC 3 cut(s) 204, 465, 749
MboI GATC 3 cut(s) 202, 463, 747
MboII GAAGA 8 cut(s) 60, 104, 257, 379, 536, 704, 749, 1026
MfeI CAATTG 1 cut(s) 776
MhlI GDGCHC 1 cut(s) 1056
MluCI AATT 8 cut(s) 58, 612, 704, 776, 810, 842, 1111, 1159
MlyI GAGTC 2 cut(s) 866, 1035
MmeI TCCRAC 1 cut(s) 1082
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 5 cut(s) 80, 306, 401, 516, 1004
MslI CAYNNNNRTG 2 cut(s) 795, 1096
MspI CCGG 1 cut(s) 745
MspR9I CCNGG 3 cut(s) 957, 984, 1254
MunI CAATTG 1 cut(s) 776
MvaI CCWGG 3 cut(s) 957, 984, 1254
MwoI GCNNNNNNNGC 3 cut(s) 368, 644, 1028
NcoI CCATGG 1 cut(s) 1023
NdeI CATATG 1 cut(s) 914
NdeII GATC 3 cut(s) 202, 463, 747
NlaIII CATG 6 cut(s) 398, 602, 833, 842, 1027, 1176
NlaIV GGNNCC 1 cut(s) 631
NmuCI GTSAC 2 cut(s) 764, 869
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 2 cut(s) 398, 833
PaeI GCATGC 1 cut(s) 398
PciI ACATGT 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 687
PfeI GAWTC 3 cut(s) 804, 895, 1149
PkrI GCNGC 3 cut(s) 345, 1030, 1201
PleI GAGTC 2 cut(s) 866, 1035
PpsI GAGTC 2 cut(s) 866, 1035
PscI ACATGT 1 cut(s) 829
PshAI GACNNNNGTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 955, 982, 1252
PspGI CCWGG 3 cut(s) 955, 982, 1252
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 742
PstI CTGCAG 1 cut(s) 909
RseI CAYNNNNRTG 2 cut(s) 795, 1096
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 5 cut(s) 80, 306, 401, 516, 1004
SatI GCNGC 3 cut(s) 344, 1029, 1200
Sau3AI GATC 3 cut(s) 202, 463, 747
Sau96I GGNCC 1 cut(s) 742
SchI GAGTC 2 cut(s) 866, 1035
ScrFI CCNGG 3 cut(s) 957, 984, 1254
SduI GDGCHC 1 cut(s) 1056
SetI ASST 8 cut(s) 265, 427, 552, 606, 640, 822, 1252, 1271
SfaNI GCATC 3 cut(s) 208, 687, 718
SfcI CTRYAG 2 cut(s) 366, 905
SinI GGWCC 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 795, 1096
SphI GCATGC 1 cut(s) 398
Sse9I AATT 8 cut(s) 58, 612, 704, 776, 810, 842, 1111, 1159
SsiI CCGC 1 cut(s) 772
SspI AATATT 2 cut(s) 513, 1181
SspMI CTAG 6 cut(s) 45, 206, 608, 824, 881, 1038
StyD4I CCNGG 3 cut(s) 955, 982, 1252
StyI CCWWGG 1 cut(s) 1023
TaaI ACNGT 3 cut(s) 25, 106, 167
TaiI ACGT 2 cut(s) 1252, 1271
TaqI TCGA 2 cut(s) 427, 588
TaqII GACCGA 2 cut(s) 32, 273
TasI AATT 8 cut(s) 58, 612, 704, 776, 810, 842, 1111, 1159
TfiI GAWTC 3 cut(s) 804, 895, 1149
Tru1I TTAA 5 cut(s) 80, 306, 401, 516, 1004
Tru9I TTAA 5 cut(s) 80, 306, 401, 516, 1004
TscAI CASTG 4 cut(s) 30, 111, 511, 679
TseFI GTSAC 2 cut(s) 764, 869
TseI GCWGC 3 cut(s) 343, 1028, 1199
Tsp45I GTSAC 2 cut(s) 764, 869
TspDTI ATGAA 7 cut(s) 855, 901, 942, 952, 990, 1153, 1170
TspGWI ACGGA 2 cut(s) 139, 312
TspRI CASTG 4 cut(s) 30, 111, 511, 679
VpaK11BI GGWCC 1 cut(s) 742
XapI RAATTY 4 cut(s) 704, 842, 1111, 1159
XbaI TCTAGA 2 cut(s) 607, 1037
XceI RCATGY 2 cut(s) 398, 833
XmiI GTMKAC 2 cut(s) 919, 994
XspI CTAG 6 cut(s) 45, 206, 608, 824, 881, 1038
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.