RchiOBHm_Chr1g0325031

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000555
Physical Location & Seq
Forward (+)
12859159 .. 12861663
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55480

Sequence Viewer

Length: 1257 bp
ATGGCTCTATTGGGAAACCCAAAACATATGGCTCTTTCATTGACGGAAACAACAACACGCCTATTTGTTACTAGATTTCTTAGACAATATAGATCTACTGGATGTTTAATCTTATTAGAAGAGGGAGGCAAAATATTCAACTTTGAGGGAAGCAAAACGGACTATTCTCTAAAATCCGTTCTCAAAGTTCATAGTCCTCAGTTTTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATCAGTGGAGATTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTTCTTGCCAACGGAGATTCTAATCCCTCAAAATCAAACAAGAAAAGGGGATGGTGGCAGCCTTTGTTTTTCACAGCAACTATAGCCACAGCCAAATATGTCTTTCAGCATGCTTTAAGGAAAAATAGTCTTACACAGGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCGCTGTTCTTGGGTGAAACAATGATGTATTCCACTGCAATATTTAAGGTCAGAATCACCAGCTTCCAGTCAGTTTACAACAATAAACACTACATGGAAGAAAATACATTAATTGTAATGTACCTTATATTTTTCTTTATCTGCCAAATTGTCATTCTTAAGACAGAAGATGAGGAACTGAATACTGCAAAGCTTAGGAAAATCTCTACTCTCATTGATAAGTTGAGTTCAGATATTGGATGTGGTTGGGGAACCTTTACTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTGAAATTTGCTAAAAAAAAAAAAAAAGCGAAGGATGCTGGACTTCAGGACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTTTAACCACACATATGATAGGATCATATCTTGCGAGATGCTAGAACATGTCGGCCATGAATTCGTGGATGAGTTTTTTTCTTGTTGCGAGTCTTTACTAGCAGAAAATGGGATTTTTGTTCTGCAGTTCATATCTATACTAGATGAACTCTATGATCAGTACAGGCGAAGTCCAGGCTTCATGAAGGAATATATGTTTCCTGGTGGATGTCTACTTTCATTAAGTAGGGTAATATCAGCCATGGCCACTTCTTCTAGACTCTGTGTGGAGCACATGGAAAACATTGGAATTCATTATTACCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGATAGACAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

418

Amino Acids

48.5

Weight (kDa)

8.88

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 148 - 416 5.4e-53 Mycolic acid cyclopropane synthetase
Methyltransf_25 PF13649 244 - 340 6.4e-16 Methyltransferase domain
Methyltransf_11 PF08241 244 - 344 2.6e-15 Methyltransferase domain
Methyltransf_12 PF08242 244 - 342 2.4e-13 Methyltransferase domain
Methyltransf_31 PF13847 244 - 361 1.7e-12 Methyltransferase domain
Methyltransf_23 PF13489 244 - 347 1.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1120
AciI CCGC 1 cut(s) 898
AclWI GGATC 2 cut(s) 881, 938
AcoI YGGCCR 2 cut(s) 961, 1152
AcsI RAATTY 4 cut(s) 824, 968, 1197, 1234
AcuI CTGAAG 1 cut(s) 848
AfaI GTAC 2 cut(s) 620, 1070
AflII CTTAAG 1 cut(s) 656
AflIII ACRYGT 1 cut(s) 955
AgsI TTSAA 2 cut(s) 139, 764
AjnI CCWGG 2 cut(s) 1081, 1108
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 2 cut(s) 561, 691
AluI AGCT 2 cut(s) 561, 691
Alw21I GWGCWC 1 cut(s) 1182
AlwI GGATC 2 cut(s) 881, 938
AoxI GGCC 2 cut(s) 961, 1152
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 4 cut(s) 824, 968, 1197, 1234
AseI ATTAAT 1 cut(s) 608
AspS9I GGNCC 1 cut(s) 868
AsuHPI GGTGA 2 cut(s) 524, 547
AvaII GGWCC 1 cut(s) 868
BalI TGGCCA 1 cut(s) 1154
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 1182
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 1083, 1110
BclI TGATCA 1 cut(s) 1063
BfaI CTAG 6 cut(s) 72, 233, 950, 1007, 1049, 1164
BfmI CTRYAG 2 cut(s) 393, 1031
BfrI CTTAAG 1 cut(s) 656
BglII AGATCT 1 cut(s) 92
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
Bme1390I CCNGG 2 cut(s) 1083, 1110
Bme18I GGWCC 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 868
BmiI GGNNCC 1 cut(s) 751
BmrFI CCNGG 2 cut(s) 1083, 1110
BmsI GCATC 4 cut(s) 235, 807, 844, 936
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 286, 1149
BsaWI WCCGGW 1 cut(s) 870
Bse1I ACTGG 4 cut(s) 103, 212, 565, 799
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 2 cut(s) 1083, 1110
BseDI CCNNGG 2 cut(s) 286, 1149
BseGI GGATG 6 cut(s) 107, 368, 743, 859, 982, 1121
BseJI GATNNNNATC 1 cut(s) 333
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 3 cut(s) 212, 473, 801
BseNI ACTGG 4 cut(s) 103, 212, 565, 799
BseXI GCAGC 1 cut(s) 382
BshFI GGCC 2 cut(s) 963, 1154
BsiHKAI GWGCWC 1 cut(s) 1182
BsiSI CCGG 1 cut(s) 871
BsnI GGCC 2 cut(s) 963, 1154
Bsp1286I GDGCHC 1 cut(s) 1182
Bsp143I GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 1 cut(s) 898
BspANI GGCC 2 cut(s) 963, 1154
BspCNI CTCAG 3 cut(s) 211, 474, 802
BspHI TCATGA 1 cut(s) 1089
BspLI GGNNCC 1 cut(s) 751
BspMAI CTGCAG 1 cut(s) 1035
BspPI GGATC 2 cut(s) 881, 938
BspTI CTTAAG 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 4 cut(s) 103, 212, 565, 799
BssECI CCNNGG 2 cut(s) 286, 1149
BssMI GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
BssT1I CCWWGG 1 cut(s) 1149
Bst2UI CCWGG 2 cut(s) 1083, 1110
Bst6I CTCTTC 1 cut(s) 114
BstAFI CTTAAG 1 cut(s) 656
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 7 cut(s) 80, 198, 482, 692, 810, 1217, 1240
BstDSI CCRYGG 1 cut(s) 1149
BstF5I GGATG 6 cut(s) 107, 368, 743, 859, 982, 1121
BstKTI GATC 6 cut(s) 95, 232, 481, 876, 933, 1066
BstMBI GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
BstMWI GCNNNNNNNGC 2 cut(s) 395, 854
BstNI CCWGG 2 cut(s) 1083, 1110
BstNSI RCATGY 2 cut(s) 425, 959
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 1081, 1108
BstSFI CTRYAG 2 cut(s) 393, 1031
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 2 cut(s) 963, 1154
BtgI CCRYGG 1 cut(s) 1149
BtsCI GGATG 6 cut(s) 107, 368, 743, 859, 982, 1121
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 3 cut(s) 262, 531, 792
Cac8I GCNNGC 1 cut(s) 423
CciI TCATGA 1 cut(s) 1089
Cfr13I GGNCC 1 cut(s) 868
Csp6I GTAC 2 cut(s) 619, 1069
CviAII CATG 7 cut(s) 422, 592, 956, 965, 1090, 1150, 1183
CviQI GTAC 2 cut(s) 619, 1069
DdeI CTNAG 7 cut(s) 80, 198, 482, 692, 810, 1217, 1240
DpnI GATC 6 cut(s) 94, 231, 480, 875, 932, 1065
DpnII GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
EaeI YGGCCR 2 cut(s) 961, 1152
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 1149
Eco47I GGWCC 1 cut(s) 868
Eco57I CTGAAG 1 cut(s) 848
EcoRI GAATTC 2 cut(s) 968, 1197
EcoRII CCWGG 2 cut(s) 1081, 1108
EcoT14I CCWWGG 1 cut(s) 1149
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 1149
FaeI CATG 7 cut(s) 425, 595, 959, 968, 1093, 1153, 1186
FalI AAGNNNNNCTT 2 cut(s) 1223, 1255
FatI CATG 7 cut(s) 421, 591, 955, 964, 1089, 1149, 1182
FauNDI CATATG 2 cut(s) 27, 922
FbaI TGATCA 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 1120
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 6 cut(s) 114, 375, 750, 866, 989, 1128
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 6 cut(s) 72, 233, 950, 1007, 1049, 1164
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 2 cut(s) 963, 1154
HapII CCGG 1 cut(s) 871
Hin1II CATG 7 cut(s) 425, 595, 959, 968, 1093, 1153, 1186
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 329, 552, 998, 1167
HpaII CCGG 1 cut(s) 871
HphI GGTGA 2 cut(s) 524, 547
Hpy166II GTNNAC 2 cut(s) 574, 1121
Hpy188I TCNGA 4 cut(s) 483, 551, 730, 811
Hpy188III TCNNGA 3 cut(s) 866, 1090, 1164
Hpy8I GTNNAC 2 cut(s) 574, 1121
HpyAV CCTTC 4 cut(s) 450, 844, 893, 1087
HpyCH4V TGCA 6 cut(s) 242, 248, 536, 686, 786, 1033
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 854
HpyF3I CTNAG 7 cut(s) 80, 198, 482, 692, 810, 1217, 1240
Hsp92II CATG 7 cut(s) 425, 595, 959, 968, 1093, 1153, 1186
Ksp22I TGATCA 1 cut(s) 1063
Kzo9I GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
LmnI GCTCC 1 cut(s) 1177
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 4 cut(s) 235, 807, 844, 936
MaeI CTAG 6 cut(s) 72, 233, 950, 1007, 1049, 1164
MaeIII GTNAC 2 cut(s) 67, 890
MalI GATC 6 cut(s) 94, 231, 480, 875, 932, 1065
MboI GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
MboII GAAGA 6 cut(s) 131, 284, 305, 608, 677, 1152
MfeI CAATTG 1 cut(s) 902
MflI RGATCY 1 cut(s) 92
MhlI GDGCHC 1 cut(s) 1182
MlsI TGGCCA 1 cut(s) 1154
MluCI AATT 7 cut(s) 609, 645, 824, 902, 968, 1197, 1234
MluNI TGGCCA 1 cut(s) 1154
MlyI GAGTC 2 cut(s) 1007, 1161
MmeI TCCRAC 1 cut(s) 1205
MnlI CCTC 9 cut(s) 115, 119, 139, 207, 281, 349, 664, 805, 1245
Mox20I TGGCCA 1 cut(s) 1154
Mph1103I ATGCAT 1 cut(s) 250
MscI TGGCCA 1 cut(s) 1154
MseI TTAA 7 cut(s) 107, 428, 543, 608, 657, 912, 1130
MslI CAYNNNNRTG 2 cut(s) 921, 1219
Msp20I TGGCCA 1 cut(s) 1154
MspCI CTTAAG 1 cut(s) 656
MspI CCGG 1 cut(s) 871
MspR9I CCNGG 2 cut(s) 1083, 1110
MunI CAATTG 1 cut(s) 902
MvaI CCWGG 2 cut(s) 1083, 1110
MwoI GCNNNNNNNGC 2 cut(s) 395, 854
NcoI CCATGG 1 cut(s) 1149
NdeI CATATG 2 cut(s) 27, 922
NdeII GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
NlaIII CATG 7 cut(s) 425, 595, 959, 968, 1093, 1153, 1186
NlaIV GGNNCC 1 cut(s) 751
NmuCI GTSAC 1 cut(s) 890
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 2 cut(s) 425, 959
PaeI GCATGC 1 cut(s) 425
PagI TCATGA 1 cut(s) 1089
PciI ACATGT 1 cut(s) 955
PfeI GAWTC 2 cut(s) 329, 552
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 2 cut(s) 1006, 1161
PpsI GAGTC 2 cut(s) 1006, 1161
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 1081, 1108
PspGI CCWGG 2 cut(s) 1081, 1108
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 1 cut(s) 868
PstI CTGCAG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 92
RsaI GTAC 2 cut(s) 620, 1070
RsaNI GTAC 2 cut(s) 619, 1069
RseI CAYNNNNRTG 2 cut(s) 921, 1219
SaqAI TTAA 7 cut(s) 107, 428, 543, 608, 657, 912, 1130
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 6 cut(s) 92, 229, 478, 873, 930, 1063
Sau96I GGNCC 1 cut(s) 868
SchI GAGTC 2 cut(s) 1007, 1161
ScrFI CCNGG 2 cut(s) 1083, 1110
SduI GDGCHC 1 cut(s) 1182
SetI ASST 7 cut(s) 292, 549, 563, 624, 693, 755, 1256
SfaNI GCATC 4 cut(s) 235, 807, 844, 936
SfcI CTRYAG 2 cut(s) 393, 1031
SinI GGWCC 1 cut(s) 868
SmiMI CAYNNNNRTG 2 cut(s) 921, 1219
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SphI GCATGC 1 cut(s) 425
Sse9I AATT 7 cut(s) 609, 645, 824, 902, 968, 1197, 1234
SsiI CCGC 1 cut(s) 898
SspI AATATT 2 cut(s) 135, 540
SspMI CTAG 6 cut(s) 72, 233, 950, 1007, 1049, 1164
StyD4I CCNGG 2 cut(s) 1081, 1108
StyI CCWWGG 1 cut(s) 1149
TaqI TCGA 1 cut(s) 454
TaqII GACCGA 1 cut(s) 300
TasI AATT 7 cut(s) 609, 645, 824, 902, 968, 1197, 1234
TatI WGTACW 1 cut(s) 1068
TfiI GAWTC 2 cut(s) 329, 552
Tru1I TTAA 7 cut(s) 107, 428, 543, 608, 657, 912, 1130
Tru9I TTAA 7 cut(s) 107, 428, 543, 608, 657, 912, 1130
TscAI CASTG 3 cut(s) 262, 538, 799
TseFI GTSAC 1 cut(s) 890
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 890
TspGWI ACGGA 4 cut(s) 59, 166, 173, 339
TspRI CASTG 3 cut(s) 262, 538, 799
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 868
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 4 cut(s) 824, 968, 1197, 1234
XbaI TCTAGA 1 cut(s) 1163
XceI RCATGY 2 cut(s) 425, 959
XmiI GTMKAC 1 cut(s) 1120
XspI CTAG 6 cut(s) 72, 233, 950, 1007, 1049, 1164
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.