Rw4G003970

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000555
Physical Location & Seq
Forward (+)
7849376 .. 7853746
4371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003970.1

Sequence Viewer

Length: 1389 bp
ATGGCTCTTTCATTAACGGAAACAGCGGCACGCTTATTTGTTACTAGATTTCTTAGACATTATATATCTACAGGATGTTTAATCTTATTAGAAGAGGGAGGCACAATGCTCACCTTTGAGGGAAGCAATATGGACTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCACTTTTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATCAGTGGAGATTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTGTTTATGCTTCTCCTTGCCAATGGAGATTCTAATGCCTCAAAATCAAACAAGAAAAGGGGATGGTGGCAGCCTTTGTTTTTCACAGCCGCTATAGCCTCAGCCAAATATGTCTTTCAACATGCTTTAAGGAAAAATAGTCTTACACAGGCTCGAAGGAATATATCTCGTCACTATGATCTGAGTAATGAACTATTTTCGCTGTTCTTGGGTGAAACAATGATGTATTCCACTGCAATATTTAAGACAAAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTCAACTCTCATTGATAAGGCTAGAATTGATGAGAACCATGAGGTTCTGGATATTGGATGTGGTTGGGGAACCTTTGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTGAAATTTGCTGAAAAAAAAGTGAAAGATGCTGGATTTCAGGACCGGATAAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTAACCACAAATATGACAGAATCATATCTTGCGAGATGCTAGAACATGTCGGCCATGACTTTTTTTTTTCTTGTTGTGAGTCTTTACTAGCAGAAAATGGGATTTTTGTTCTGCAGTTCATATCTATACTGGATGAACTCTATGATGAGTACACGCGAAGTCCAGGCTTCATGAAGGAATATATATTTCCTGGTGGATGTCTGCTTTCATTGAGTAGGGTAATATCAGCCATGGCCACTTCTTCTAGACTCTGTGTGGAGCACATGGAAAACATTGGAAGTCATTATTACCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGAGAGACAGAGGAATCTAATAATTATGACTGCTACACTTTTAGCTTTTAATAAAATGCAATTACTCTTTTGTGTTTATAGTGAAATACTTGCTCTTGGATTCAATGAAAATTTCATTCGGACATGGGAATATTATTTTGATTATTGCGCTGCTGGTTTCAAGTCATATACACTTAGAGATTACCAGGTAACTTCTATAGTCCACACATTTTCTGAATTTCTATTGTGTTTTCACGTCCTCGTAATCAACAATCCATATAAGGGTTTCCCTTCAGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

462

Amino Acids

53.28

Weight (kDa)

6.88

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 139 - 379 9.1e-68 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 185 - 301 1.1e-09 Methyltransferase domain
Methyltransf_31 PF13847 197 - 315 4.5e-15 Methyltransferase domain
MTS PF05175 197 - 271 6.1e-06 Methyltransferase small domain
Methyltransf_25 PF13649 202 - 295 7e-16 Methyltransferase domain
Methyltransf_11 PF08241 203 - 299 1.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 203 - 297 1.6e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 940
AciI CCGC 3 cut(s) 26, 363, 772
AcoI YGGCCR 2 cut(s) 835, 1017
AcsI RAATTY 4 cut(s) 704, 1099, 1216, 1322
AcuI CTGAAG 1 cut(s) 1362
AfaI GTAC 1 cut(s) 935
AfiI CCNNNNNNNGG 1 cut(s) 1367
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 4 cut(s) 392, 644, 1210, 1267
AjiI CACGTC 1 cut(s) 1342
AjnI CCWGG 3 cut(s) 946, 973, 1290
AluBI AGCT 2 cut(s) 550, 1151
AluI AGCT 2 cut(s) 550, 1151
Alw21I GWGCWC 1 cut(s) 1047
Alw26I GTCTC 1 cut(s) 1105
AoxI GGCC 2 cut(s) 835, 1017
ApeKI GCWGC 2 cut(s) 343, 1256
ApoI RAATTY 4 cut(s) 704, 1099, 1216, 1322
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 1 cut(s) 742
AsuHPI GGTGA 2 cut(s) 103, 497
AvaII GGWCC 1 cut(s) 742
BalI TGGCCA 1 cut(s) 1019
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 1 cut(s) 1047
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 2 cut(s) 355, 1243
BccI CCATC 1 cut(s) 330
BciT130I CCWGG 3 cut(s) 948, 975, 1292
BcoDI GTCTC 1 cut(s) 1105
BfaI CTAG 6 cut(s) 45, 206, 582, 824, 872, 1029
BfmI CTRYAG 4 cut(s) 69, 366, 896, 1302
BisI GCNGC 4 cut(s) 27, 344, 363, 1257
BlsI GCNGC 4 cut(s) 28, 345, 364, 1258
Bme1390I CCNGG 3 cut(s) 948, 975, 1292
Bme18I GGWCC 1 cut(s) 742
BmgBI CACGTC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 742
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 3 cut(s) 948, 975, 1292
BmsI GCATC 4 cut(s) 208, 687, 718, 810
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 2 cut(s) 373, 551
BsaJI CCNNGG 2 cut(s) 259, 1014
BsaWI WCCGGW 1 cut(s) 744
Bsc4I CCNNNNNNNGG 1 cut(s) 1367
Bse1I ACTGG 3 cut(s) 185, 679, 918
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 3 cut(s) 948, 975, 1292
BseDI CCNNGG 2 cut(s) 259, 1014
BseGI GGATG 5 cut(s) 80, 341, 623, 922, 986
BseLI CCNNNNNNNGG 1 cut(s) 1367
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 3 cut(s) 387, 446, 681
BseNI ACTGG 3 cut(s) 185, 679, 918
BseXI GCAGC 2 cut(s) 355, 1243
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 940
BshFI GGCC 2 cut(s) 837, 1019
BsiHKAI GWGCWC 1 cut(s) 1047
BsiSI CCGG 1 cut(s) 745
BslI CCNNNNNNNGG 1 cut(s) 1367
BsmAI GTCTC 1 cut(s) 1105
BsnI GGCC 2 cut(s) 837, 1019
Bsp1286I GDGCHC 1 cut(s) 1047
Bsp143I GATC 2 cut(s) 202, 451
Bsp19I CCATGG 1 cut(s) 1014
BspACI CCGC 3 cut(s) 26, 363, 772
BspANI GGCC 2 cut(s) 837, 1019
BspCNI CTCAG 3 cut(s) 386, 447, 682
BspFNI CGCG 1 cut(s) 940
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 900
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 3 cut(s) 185, 679, 918
BssECI CCNNGG 2 cut(s) 259, 1014
BssMI GATC 2 cut(s) 202, 451
BssT1I CCWWGG 1 cut(s) 1014
Bst2UI CCWGG 3 cut(s) 948, 975, 1292
Bst4CI ACNGT 2 cut(s) 137, 167
Bst6I CTCTTC 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 8 cut(s) 53, 373, 455, 551, 690, 1082, 1105, 1280
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 5 cut(s) 80, 341, 623, 922, 986
BstFNI CGCG 1 cut(s) 940
BstHHI GCGC 1 cut(s) 1256
BstKTI GATC 2 cut(s) 205, 454
BstMAI GTCTC 1 cut(s) 1105
BstMBI GATC 2 cut(s) 202, 451
BstMWI GCNNNNNNNGC 2 cut(s) 368, 644
BstNI CCWGG 3 cut(s) 948, 975, 1292
BstNSI RCATGY 2 cut(s) 398, 833
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 3 cut(s) 946, 973, 1290
BstSFI CTRYAG 4 cut(s) 69, 366, 896, 1302
BstUI CGCG 1 cut(s) 940
BstV1I GCAGC 2 cut(s) 355, 1243
BstV2I GAAGAC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 837, 1019
BtgI CCRYGG 1 cut(s) 1014
BtrI CACGTC 1 cut(s) 1342
BtsCI GGATG 5 cut(s) 80, 341, 623, 922, 986
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 235, 504, 672
Cac8I GCNNGC 1 cut(s) 31
CciI TCATGA 1 cut(s) 954
CfoI GCGC 1 cut(s) 1256
Cfr13I GGNCC 1 cut(s) 742
CsiI ACCWGGT 1 cut(s) 1290
Csp6I GTAC 1 cut(s) 934
CviAII CATG 8 cut(s) 395, 599, 830, 839, 955, 1015, 1048, 1230
CviQI GTAC 1 cut(s) 934
DdeI CTNAG 8 cut(s) 53, 373, 455, 551, 690, 1082, 1105, 1280
DpnI GATC 2 cut(s) 204, 453
DpnII GATC 2 cut(s) 202, 451
EaeI YGGCCR 2 cut(s) 835, 1017
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 1014
Eco47I GGWCC 1 cut(s) 742
Eco57I CTGAAG 1 cut(s) 1362
EcoRII CCWGG 3 cut(s) 946, 973, 1290
EcoT14I CCWWGG 1 cut(s) 1014
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 1014
FaeI CATG 8 cut(s) 398, 602, 833, 842, 958, 1018, 1051, 1233
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FatI CATG 8 cut(s) 394, 598, 829, 838, 954, 1014, 1047, 1229
Fnu4HI GCNGC 4 cut(s) 27, 344, 363, 1257
FokI GGATG 5 cut(s) 87, 348, 630, 929, 993
Fsp4HI GCNGC 4 cut(s) 27, 344, 363, 1257
FspBI CTAG 6 cut(s) 45, 206, 582, 824, 872, 1029
GlaI GCGC 1 cut(s) 1255
GluI GCNGC 4 cut(s) 27, 344, 363, 1257
HaeIII GGCC 2 cut(s) 837, 1019
HapII CCGG 1 cut(s) 745
HhaI GCGC 1 cut(s) 1256
Hin1II CATG 8 cut(s) 398, 602, 833, 842, 958, 1018, 1051, 1233
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HinfI GANTC 6 cut(s) 302, 804, 863, 1032, 1120, 1206
HpaII CCGG 1 cut(s) 745
HphI GGTGA 2 cut(s) 103, 497
Hpy166II GTNNAC 3 cut(s) 163, 936, 1309
Hpy188I TCNGA 4 cut(s) 456, 691, 1227, 1321
Hpy188III TCNNGA 4 cut(s) 608, 740, 955, 1029
Hpy8I GTNNAC 3 cut(s) 163, 936, 1309
HpyAV CCTTC 5 cut(s) 423, 767, 792, 952, 1386
HpyCH4III ACNGT 2 cut(s) 137, 167
HpyCH4IV ACGT 1 cut(s) 1341
HpyCH4V TGCA 7 cut(s) 215, 221, 509, 545, 666, 898, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 644
HpyF3I CTNAG 8 cut(s) 53, 373, 455, 551, 690, 1082, 1105, 1280
HpySE526I ACGT 1 cut(s) 1341
Hsp92II CATG 8 cut(s) 398, 602, 833, 842, 958, 1018, 1051, 1233
HspAI GCGC 1 cut(s) 1254
Kzo9I GATC 2 cut(s) 202, 451
LmnI GCTCC 1 cut(s) 1042
Lsp1109I GCAGC 2 cut(s) 355, 1243
LweI GCATC 4 cut(s) 208, 687, 718, 810
MabI ACCWGGT 1 cut(s) 1290
MaeI CTAG 6 cut(s) 45, 206, 582, 824, 872, 1029
MaeII ACGT 1 cut(s) 1341
MaeIII GTNAC 4 cut(s) 40, 443, 764, 1294
MalI GATC 2 cut(s) 204, 453
MboI GATC 2 cut(s) 202, 451
MboII GAAGA 3 cut(s) 104, 257, 1017
MfeI CAATTG 1 cut(s) 776
MhlI GDGCHC 1 cut(s) 1047
MlsI TGGCCA 1 cut(s) 1019
MluCI AATT 8 cut(s) 585, 704, 776, 1099, 1128, 1166, 1216, 1322
MluNI TGGCCA 1 cut(s) 1019
MlyI GAGTC 2 cut(s) 872, 1026
MmeI TCCRAC 1 cut(s) 1070
Mox20I TGGCCA 1 cut(s) 1019
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 1019
MseI TTAA 5 cut(s) 14, 80, 401, 516, 1155
MslI CAYNNNNRTG 2 cut(s) 795, 1084
Msp20I TGGCCA 1 cut(s) 1019
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 745
MspR9I CCNGG 3 cut(s) 948, 975, 1292
MunI CAATTG 1 cut(s) 776
MvaI CCWGG 3 cut(s) 948, 975, 1292
MvnI CGCG 1 cut(s) 940
MwoI GCNNNNNNNGC 2 cut(s) 368, 644
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 2 cut(s) 202, 451
NlaIII CATG 8 cut(s) 398, 602, 833, 842, 958, 1018, 1051, 1233
NlaIV GGNNCC 1 cut(s) 631
NmuCI GTSAC 2 cut(s) 443, 764
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 2 cut(s) 398, 833
PagI TCATGA 1 cut(s) 954
PciI ACATGT 1 cut(s) 829
PfeI GAWTC 4 cut(s) 302, 804, 1120, 1206
PkrI GCNGC 4 cut(s) 28, 345, 364, 1258
PleI GAGTC 2 cut(s) 871, 1026
PpsI GAGTC 2 cut(s) 871, 1026
PscI ACATGT 1 cut(s) 829
PshAI GACNNNNGTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 946, 973, 1290
PspGI CCWGG 3 cut(s) 946, 973, 1290
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 742
PstI CTGCAG 1 cut(s) 900
RsaI GTAC 1 cut(s) 935
RsaNI GTAC 1 cut(s) 934
RseI CAYNNNNRTG 2 cut(s) 795, 1084
SaqAI TTAA 5 cut(s) 14, 80, 401, 516, 1155
SatI GCNGC 4 cut(s) 27, 344, 363, 1257
Sau3AI GATC 2 cut(s) 202, 451
Sau96I GGNCC 1 cut(s) 742
SchI GAGTC 2 cut(s) 872, 1026
ScrFI CCNGG 3 cut(s) 948, 975, 1292
SduI GDGCHC 1 cut(s) 1047
SetI ASST 8 cut(s) 116, 265, 552, 606, 635, 1153, 1296, 1344
SexAI ACCWGGT 1 cut(s) 1290
SfaNI GCATC 4 cut(s) 208, 687, 718, 810
SfcI CTRYAG 4 cut(s) 69, 366, 896, 1302
SinI GGWCC 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 795, 1084
Sse9I AATT 8 cut(s) 585, 704, 776, 1099, 1128, 1166, 1216, 1322
SsiI CCGC 3 cut(s) 26, 363, 772
SspI AATATT 2 cut(s) 513, 1238
SspMI CTAG 6 cut(s) 45, 206, 582, 824, 872, 1029
StyD4I CCNGG 3 cut(s) 946, 973, 1290
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 2 cut(s) 137, 167
TaiI ACGT 1 cut(s) 1344
TaqI TCGA 1 cut(s) 427
TaqII GACCGA 1 cut(s) 273
TasI AATT 8 cut(s) 585, 704, 776, 1099, 1128, 1166, 1216, 1322
TatI WGTACW 1 cut(s) 933
TauI GCSGC 2 cut(s) 29, 365
TfiI GAWTC 4 cut(s) 302, 804, 1120, 1206
Tru1I TTAA 5 cut(s) 14, 80, 401, 516, 1155
Tru9I TTAA 5 cut(s) 14, 80, 401, 516, 1155
TscAI CASTG 3 cut(s) 235, 511, 679
TseFI GTSAC 2 cut(s) 443, 764
TseI GCWGC 2 cut(s) 343, 1256
Tsp45I GTSAC 2 cut(s) 443, 764
TspDTI ATGAA 8 cut(s) 477, 892, 933, 943, 971, 981, 1210, 1227
TspGWI ACGGA 2 cut(s) 32, 139
TspRI CASTG 3 cut(s) 235, 511, 679
VpaK11BI GGWCC 1 cut(s) 742
XapI RAATTY 4 cut(s) 704, 1099, 1216, 1322
XbaI TCTAGA 1 cut(s) 1028
XceI RCATGY 2 cut(s) 398, 833
XspI CTAG 6 cut(s) 45, 206, 582, 824, 872, 1029
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.