RchiOBHm_Chr4g0394801

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000555
Physical Location & Seq
Reverse (-)
10544771 .. 10550315
5545 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36727

Sequence Viewer

Length: 2376 bp
ATGAAAGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGCGTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCCAGGATTGTCACATGTGATGGTGTTGATTTGGACCTTGGTTTCATGGTCTTCAATCATGTGATGTATCCAAATATGATGGAGTTCTTTGAGAACCTTGGAGTTGATATGGAGACATGCGACATGTCCTTCTCAGTGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCGAAATAGTTTTTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGGTACGTAACTGAAATGCATGCCATGTCTCAAAATGTCCTATTGAGTTATCTTGAGGTGCTTGAGAGTAACACAGACATTGACCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTAGGAAGGCTTACCTTATTCCAATATGTAGTTCAATCTTGTCGTGTCCTTCAGAAGGAGTTATAAGCTTGTCAGCTTACTCTGTGCTTTCGGTTTGTCGCAATTATCAACTACTTCAGCTATTTGGCGGCCCACAGTGGCTTACCGTCAAATGGCGTTCACAGTGTTATGTGAAGAAGGTGAAAGAAGTGTTGGAGAGTAAGGGTTGTCAGATAAGAACCGGCTGTGAGGTACATAAAGTTTCCACAACTGATGAGGGTGGAACTGTTTTTACTGGGGATGGTTTTGAGGAAATTTACAATGGATGCATTATTGCTATTCAAGCCCCCAAAGCCGTGAGAATATTAGGAGATCAGGCAACACCTGATGAATTGAGAATACTTGGCGCCTTCCAATATGTATACCGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCAAAACCTAGCAGCATGGAGTGCATTGAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGGACCTCGGTGAAATAAGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCAGCATCAAAAGCTTCGCTGGATCTTCCTCAAATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGGCTGGAATGGCTGCAGCCCATGGTATGCTTGGAAAAGGTTGTGCCCTGTTAAGAAACCCAAAACATATGGCTTTTTCAACGACTGAAACAGCGGCACGCTTGTTTGTTACTAGATTTCTTAGACATTATATATCTACCGGATGTTTGATCTTATTAGAAGAGGGCGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACGAAGGCTGATTTAGGCATTGCAGATGCATATATCGATGGTGATTTTTCTTTCAATGATAAAGACCAAGGTCTTCTAAATCTTATTATGCCAGGTCTCAATACATTGTTGGTTTTATGTAGGGGAAGGTGGCAGCCTTTGTTTTTCACAGCCACCATAGGCTCTATAGCCTCAGCCAGATACTTCTTTCAGCATGCTTTGAAGAAAAATAGTCTTGCACAGGCTCGAAGGAATATATCTCATCATTATGATCTGCGTAATGAACTATTTTCGTTGTTCTTGGGCGAAACAATGATGTATTCCGCTGCAATATTTAAGACAGAAGATGAGGAATTGAATACTGCACAGCTTAGGAAAATCTCTACTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTAGATATTGGTTGTGGTTGGGGAACCTTAGCTATTGAAGCAGTTAAACGAACGGGCTGCAGATACACCGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGATGTTAGAACATGTCGGCCATGAATTTATGGATGAGTTCTTTTCTTGCTGTGAGTCTGTATTGGCAAAAAATGGGCTTTTTATTCTCCAGTTCACATCTATAGCGGATGAACGCTATGATGAGTACAGGCGAAGTTCAGGCTTCTTAAACGAATATATATTTCCTGGTGGATGTCTACTTTCATTAAGTAGGGTAACATCAGCCATGGCGGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATCGGAATTCACTATTACCAAACACTACGATGTTGGAGAAAGAATTTTTTAGAGAGGCAGAGGTAA

Protein Analysis

791

Amino Acids

89.41

Weight (kDa)

6.57

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 5.8e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 2.2e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 293 2.6e-16 Flavin containing amine oxidoreductase
CMAS PF02353 581 - 687 1.3e-33 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 638 - 688 2.2e-06 Methyltransferase domain
Methyltransf_25 PF13649 643 - 688 3.2e-08 Methyltransferase domain
CMAS PF02353 687 - 789 2.6e-19 Mycolic acid cyclopropane synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 911
AccI GTMKAC 2 cut(s) 927, 2239
AciI CCGC 6 cut(s) 654, 1358, 1431, 1827, 2168, 2273
AclWI GGATC 1 cut(s) 1191
AcoI YGGCCR 2 cut(s) 109, 2080
AcsI RAATTY 7 cut(s) 819, 1194, 1212, 2027, 2087, 2316, 2353
AcuI CTGAAG 4 cut(s) 561, 626, 1037, 2045
AcyI GRCGYC 1 cut(s) 912
AfaI GTAC 4 cut(s) 395, 759, 1012, 2189
AfiI CCNNNNNNNGG 2 cut(s) 581, 678
AflII CTTAAG 1 cut(s) 1127
AflIII ACRYGT 3 cut(s) 128, 237, 2074
AjnI CCWGG 5 cut(s) 102, 116, 1233, 1615, 2227
AjuI GAANNNNNNNTTGG 6 cut(s) 470, 502, 541, 573, 701, 733
AluBI AGCT 8 cut(s) 306, 594, 602, 646, 1051, 1175, 1873, 1961
AluI AGCT 8 cut(s) 306, 594, 602, 646, 1051, 1175, 1873, 1961
Alw21I GWGCWC 1 cut(s) 2301
Alw26I GTCTC 4 cut(s) 46, 221, 423, 1625
AlwI GGATC 1 cut(s) 1191
AoxI GGCC 3 cut(s) 109, 655, 2080
ApeKI GCWGC 7 cut(s) 980, 1163, 1277, 1280, 1657, 1829, 1986
ApoI RAATTY 7 cut(s) 819, 1194, 1212, 2027, 2087, 2316, 2353
Asp700I GAANNNNTTC 2 cut(s) 78, 329
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 3 cut(s) 148, 656, 1057
AsuHPI GGTGA 5 cut(s) 358, 718, 1076, 1435, 1577
AvaII GGWCC 2 cut(s) 148, 1057
BaeGI GKGCMC 1 cut(s) 1312
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 911
BbsI GAAGAC 2 cut(s) 157, 1589
Bbv12I GWGCWC 1 cut(s) 2301
BbvCI CCTCAGC 1 cut(s) 1696
BbvI GCAGC 7 cut(s) 992, 1175, 1264, 1292, 1669, 1816, 1973
BccI CCATC 6 cut(s) 128, 187, 800, 1152, 1250, 1556
BceAI ACGGC 1 cut(s) 845
BcgI CGANNNNNNTGC 2 cut(s) 1968, 2002
BciT130I CCWGG 5 cut(s) 104, 118, 1235, 1617, 2229
BciVI GTATCC 1 cut(s) 192
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 4 cut(s) 46, 221, 423, 1625
BfaI CTAG 5 cut(s) 977, 1377, 1905, 1931, 2283
BfmI CTRYAG 4 cut(s) 1278, 1689, 1987, 2163
BfoI RGCGCY 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 1127
BfuI GTATCC 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 2273
BmcAI AGTACT 1 cut(s) 1012
Bme1390I CCNGG 5 cut(s) 104, 118, 1235, 1617, 2229
Bme18I GGWCC 2 cut(s) 148, 1057
BmgT120I GGNCC 3 cut(s) 148, 656, 1057
BmiI GGNNCC 2 cut(s) 913, 1954
BmrFI CCNGG 5 cut(s) 104, 118, 1235, 1617, 2229
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 5 cut(s) 821, 1175, 1540, 2010, 2041
BmuI ACTGGG 1 cut(s) 810
BoxI GACNNNNGTC 1 cut(s) 1593
BpiI GAAGAC 2 cut(s) 157, 1589
BpmI CTGGAG 1 cut(s) 2135
Bpu10I CCTNAGC 3 cut(s) 1696, 1874, 1957
BpuEI CTTGAG 2 cut(s) 464, 473
Bsa29I ATCGAT 2 cut(s) 1560, 1911
BsaAI YACGTR 1 cut(s) 397
BsaHI GRCGYC 1 cut(s) 912
BsaI GGTCTC 2 cut(s) 46, 1625
BsaJI CCNNGG 9 cut(s) 103, 151, 211, 486, 1060, 1234, 1285, 1591, 2268
BsaWI WCCGGW 2 cut(s) 474, 1403
Bsc4I CCNNNNNNNGG 2 cut(s) 581, 678
Bse118I RCCGGY 2 cut(s) 746, 1997
Bse1I ACTGG 5 cut(s) 805, 1043, 1148, 1517, 2152
Bse3DI GCAATG 1 cut(s) 1542
BseBI CCWGG 5 cut(s) 104, 118, 1235, 1617, 2229
BseCI ATCGAT 2 cut(s) 1560, 1911
BseDI CCNNGG 9 cut(s) 103, 151, 211, 486, 1060, 1234, 1285, 1591, 2268
BseGI GGATG 8 cut(s) 811, 836, 1144, 1261, 1412, 2101, 2176, 2240
BseLI CCNNNNNNNGG 2 cut(s) 581, 678
BseMI GCAATG 1 cut(s) 1542
BseMII CTCAG 5 cut(s) 57, 261, 1517, 1710, 2004
BseNI ACTGG 5 cut(s) 805, 1043, 1148, 1517, 2152
BseSI GKGCMC 1 cut(s) 1312
BseXI GCAGC 7 cut(s) 992, 1175, 1264, 1292, 1669, 1816, 1973
BsgI GTGCAG 1 cut(s) 1851
BshFI GGCC 3 cut(s) 111, 657, 2082
BshNI GGYRCC 1 cut(s) 911
BshVI ATCGAT 2 cut(s) 1560, 1911
BsiHKAI GWGCWC 1 cut(s) 2301
BsiSI CCGG 4 cut(s) 475, 747, 1404, 1998
BslI CCNNNNNNNGG 2 cut(s) 581, 678
BsmAI GTCTC 4 cut(s) 46, 221, 423, 1625
BsnI GGCC 3 cut(s) 111, 657, 2082
Bso31I GGTCTC 2 cut(s) 46, 1625
Bsp1286I GDGCHC 2 cut(s) 1312, 2301
Bsp143I GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
Bsp19I CCATGG 2 cut(s) 1285, 2268
BspACI CCGC 6 cut(s) 654, 1358, 1431, 1827, 2168, 2273
BspANI GGCC 3 cut(s) 111, 657, 2082
BspCNI CTCAG 5 cut(s) 56, 260, 1516, 1709, 2005
BspDI ATCGAT 2 cut(s) 1560, 1911
BspLI GGNNCC 2 cut(s) 913, 1954
BspMAI CTGCAG 2 cut(s) 1282, 1991
BspPI GGATC 1 cut(s) 1191
BspT107I GGYRCC 1 cut(s) 911
BspTI CTTAAG 1 cut(s) 1127
BspTNI GGTCTC 2 cut(s) 46, 1625
BsrDI GCAATG 1 cut(s) 1542
BsrFI RCCGGY 2 cut(s) 746, 1997
BsrI ACTGG 5 cut(s) 805, 1043, 1148, 1517, 2152
BssAI RCCGGY 2 cut(s) 746, 1997
BssECI CCNNGG 9 cut(s) 103, 151, 211, 486, 1060, 1234, 1285, 1591, 2268
BssMI GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
BssNAI GTATAC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 912
BssT1I CCWWGG 6 cut(s) 151, 211, 486, 1285, 1591, 2268
Bst1107I GTATAC 1 cut(s) 928
Bst2UI CCWGG 5 cut(s) 104, 118, 1235, 1617, 2229
Bst4CI ACNGT 7 cut(s) 663, 673, 690, 793, 932, 1469, 1499
Bst6I CTCTTC 1 cut(s) 1419
BstACI GRCGYC 1 cut(s) 912
BstAFI CTTAAG 1 cut(s) 1127
BstAPI GCANNNNNTGC 1 cut(s) 989
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 3 cut(s) 411, 1363, 1719
BstDEI CTNAG 8 cut(s) 43, 247, 1385, 1503, 1696, 1874, 1957, 2013
BstDSI CCRYGG 2 cut(s) 1285, 2268
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 8 cut(s) 811, 836, 1144, 1261, 1412, 2101, 2176, 2240
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 6 cut(s) 390, 880, 1105, 1186, 1416, 1777
BstMAI GTCTC 4 cut(s) 46, 221, 423, 1625
BstMBI GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
BstMWI GCNNNNNNNGC 8 cut(s) 848, 857, 989, 1048, 1172, 1274, 1967, 2273
BstNI CCWGG 5 cut(s) 104, 118, 1235, 1617, 2229
BstNSI RCATGY 6 cut(s) 132, 234, 241, 413, 1721, 2078
BstPAI GACNNNNGTC 1 cut(s) 1593
BstSCI CCNGG 5 cut(s) 102, 116, 1233, 1615, 2227
BstSFI CTRYAG 4 cut(s) 1278, 1689, 1987, 2163
BstSLI GKGCMC 1 cut(s) 1312
BstSNI TACGTA 1 cut(s) 397
BstV1I GCAGC 7 cut(s) 992, 1175, 1264, 1292, 1669, 1816, 1973
BstV2I GAAGAC 2 cut(s) 157, 1589
BstX2I RGATCY 1 cut(s) 1183
BstXI CCANNNNNNTGG 3 cut(s) 345, 1241, 1256
BstYI RGATCY 1 cut(s) 1183
BstZ17I GTATAC 1 cut(s) 928
Bsu15I ATCGAT 2 cut(s) 1560, 1911
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 3 cut(s) 111, 657, 2082
BsuTUI ATCGAT 2 cut(s) 1560, 1911
BtgI CCRYGG 2 cut(s) 1285, 2268
BtsCI GGATG 8 cut(s) 811, 836, 1144, 1261, 1412, 2101, 2176, 2240
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 4 cut(s) 18, 255, 668, 695
Cac8I GCNNGC 3 cut(s) 411, 1363, 1719
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 2 cut(s) 746, 1997
Cfr13I GGNCC 3 cut(s) 148, 656, 1057
ClaI ATCGAT 2 cut(s) 1560, 1911
Csp6I GTAC 4 cut(s) 394, 758, 1011, 2188
CviQI GTAC 4 cut(s) 394, 758, 1011, 2188
DdeI CTNAG 8 cut(s) 43, 247, 1385, 1503, 1696, 1874, 1957, 2013
DinI GGCGCC 1 cut(s) 913
DpnI GATC 6 cut(s) 389, 879, 1104, 1185, 1415, 1776
DpnII GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
EaeI YGGCCR 2 cut(s) 109, 2080
Eam1104I CTCTTC 1 cut(s) 1419
EarI CTCTTC 1 cut(s) 1419
Eco105I TACGTA 1 cut(s) 397
Eco130I CCWWGG 6 cut(s) 151, 211, 486, 1285, 1591, 2268
Eco31I GGTCTC 2 cut(s) 46, 1625
Eco47I GGWCC 2 cut(s) 148, 1057
Eco57I CTGAAG 4 cut(s) 561, 626, 1037, 2045
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 1057
EcoRI GAATTC 1 cut(s) 2316
EcoRII CCWGG 5 cut(s) 102, 116, 1233, 1615, 2227
EcoT14I CCWWGG 6 cut(s) 151, 211, 486, 1285, 1591, 2268
EcoT22I ATGCAT 3 cut(s) 411, 836, 1555
EgeI GGCGCC 1 cut(s) 913
EheI GGCGCC 1 cut(s) 913
ErhI CCWWGG 6 cut(s) 151, 211, 486, 1285, 1591, 2268
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FauNDI CATATG 1 cut(s) 1332
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 2 cut(s) 927, 2239
FokI GGATG 8 cut(s) 818, 843, 1131, 1268, 1419, 2108, 2183, 2247
FspBI CTAG 5 cut(s) 977, 1377, 1905, 1931, 2283
GlaI GCGC 1 cut(s) 913
GsuI CTGGAG 1 cut(s) 2135
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 3 cut(s) 111, 657, 2082
HapII CCGG 4 cut(s) 475, 747, 1404, 1998
HhaI GCGC 1 cut(s) 914
Hin1I GRCGYC 1 cut(s) 912
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HincII GTYRAC 2 cut(s) 1076, 1137
HindII GTYRAC 2 cut(s) 1076, 1137
HindIII AAGCTT 3 cut(s) 304, 592, 1173
HinfI GANTC 4 cut(s) 503, 1908, 2117, 2286
HpaII CCGG 4 cut(s) 475, 747, 1404, 1998
HphI GGTGA 5 cut(s) 358, 718, 1076, 1435, 1577
Hpy166II GTNNAC 8 cut(s) 686, 928, 1076, 1137, 1443, 1495, 2157, 2240
Hpy188I TCNGA 6 cut(s) 520, 580, 738, 2014, 2064, 2315
Hpy188III TCNNGA 8 cut(s) 346, 359, 443, 950, 1055, 1100, 1931, 2283
Hpy8I GTNNAC 8 cut(s) 686, 928, 1076, 1137, 1443, 1495, 2157, 2240
HpyCH4III ACNGT 7 cut(s) 663, 673, 690, 793, 932, 1469, 1499
HpyCH4IV ACGT 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 8 cut(s) 848, 857, 989, 1048, 1172, 1274, 1967, 2273
HpyF3I CTNAG 8 cut(s) 43, 247, 1385, 1503, 1696, 1874, 1957, 2013
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 912
HspAI GCGC 1 cut(s) 912
KasI GGCGCC 1 cut(s) 911
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
LmnI GCTCC 2 cut(s) 1226, 2296
Lsp1109I GCAGC 7 cut(s) 992, 1175, 1264, 1292, 1669, 1816, 1973
LweI GCATC 5 cut(s) 821, 1175, 1540, 2010, 2041
MaeI CTAG 5 cut(s) 977, 1377, 1905, 1931, 2283
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 8 cut(s) 124, 397, 458, 504, 950, 1087, 1372, 2257
MalI GATC 6 cut(s) 389, 879, 1104, 1185, 1415, 1776
MboI GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
MboII GAAGA 9 cut(s) 157, 334, 712, 1178, 1436, 1589, 1738, 1859, 2271
MflI RGATCY 1 cut(s) 1183
MhlI GDGCHC 2 cut(s) 1312, 2301
MlsI TGGCCA 1 cut(s) 111
MluNI TGGCCA 1 cut(s) 111
Mly113I GGCGCC 1 cut(s) 912
MlyI GAGTC 3 cut(s) 512, 2126, 2280
MmeI TCCRAC 2 cut(s) 699, 2324
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 3 cut(s) 411, 836, 1555
MroXI GAANNNNTTC 2 cut(s) 78, 329
MscI TGGCCA 1 cut(s) 111
MseI TTAA 9 cut(s) 375, 962, 1128, 1316, 1839, 1974, 2025, 2210, 2249
MslI CAYNNNNRTG 3 cut(s) 1254, 1770, 2338
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 1358, 1829
MspCI CTTAAG 1 cut(s) 1127
MspI CCGG 4 cut(s) 475, 747, 1404, 1998
MspR9I CCNGG 5 cut(s) 104, 118, 1235, 1617, 2229
MvaI CCWGG 5 cut(s) 104, 118, 1235, 1617, 2229
MwoI GCNNNNNNNGC 8 cut(s) 848, 857, 989, 1048, 1172, 1274, 1967, 2273
NarI GGCGCC 1 cut(s) 912
NcoI CCATGG 2 cut(s) 1285, 2268
NdeI CATATG 1 cut(s) 1332
NdeII GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
NlaIV GGNNCC 2 cut(s) 913, 1954
NmuCI GTSAC 4 cut(s) 124, 504, 950, 1087
NsiI ATGCAT 3 cut(s) 411, 836, 1555
NspI RCATGY 6 cut(s) 132, 234, 241, 413, 1721, 2078
PaeI GCATGC 2 cut(s) 413, 1721
PciI ACATGT 3 cut(s) 128, 237, 2074
PdmI GAANNNNTTC 2 cut(s) 78, 329
PfeI GAWTC 1 cut(s) 1908
PleI GAGTC 3 cut(s) 511, 2125, 2280
PluTI GGCGCC 1 cut(s) 915
PpsI GAGTC 3 cut(s) 511, 2125, 2280
Ppu21I YACGTR 1 cut(s) 397
PpuMI RGGWCCY 1 cut(s) 1057
PscI ACATGT 3 cut(s) 128, 237, 2074
PshAI GACNNNNGTC 1 cut(s) 1593
PsiI TTATAA 1 cut(s) 590
Psp5II RGGWCCY 1 cut(s) 1057
Psp6I CCWGG 5 cut(s) 102, 116, 1233, 1615, 2227
PspGI CCWGG 5 cut(s) 102, 116, 1233, 1615, 2227
PspN4I GGNNCC 2 cut(s) 913, 1954
PspPI GGNCC 3 cut(s) 148, 656, 1057
PspPPI RGGWCCY 1 cut(s) 1057
PsrI GAACNNNNNNTAC 2 cut(s) 781, 813
PstI CTGCAG 2 cut(s) 1282, 1991
PsuI RGATCY 1 cut(s) 1183
RsaI GTAC 4 cut(s) 395, 759, 1012, 2189
RsaNI GTAC 4 cut(s) 394, 758, 1011, 2188
RseI CAYNNNNRTG 3 cut(s) 1254, 1770, 2338
SaqAI TTAA 9 cut(s) 375, 962, 1128, 1316, 1839, 1974, 2025, 2210, 2249
Sau3AI GATC 6 cut(s) 387, 877, 1102, 1183, 1413, 1774
Sau96I GGNCC 3 cut(s) 148, 656, 1057
ScaI AGTACT 1 cut(s) 1012
SchI GAGTC 3 cut(s) 512, 2126, 2280
ScrFI CCNGG 5 cut(s) 104, 118, 1235, 1617, 2229
SduI GDGCHC 2 cut(s) 1312, 2301
SfaNI GCATC 5 cut(s) 821, 1175, 1540, 2010, 2041
SfcI CTRYAG 4 cut(s) 1278, 1689, 1987, 2163
SfoI GGCGCC 1 cut(s) 913
SinI GGWCC 2 cut(s) 148, 1057
SmiMI CAYNNNNRTG 3 cut(s) 1254, 1770, 2338
SmlI CTYRAG 3 cut(s) 443, 452, 1127
SmoI CTYRAG 3 cut(s) 443, 452, 1127
SnaBI TACGTA 1 cut(s) 397
SphI GCATGC 2 cut(s) 413, 1721
SsiI CCGC 6 cut(s) 654, 1358, 1431, 1827, 2168, 2273
SspDI GGCGCC 1 cut(s) 911
SspI AATATT 2 cut(s) 870, 1836
SspMI CTAG 5 cut(s) 977, 1377, 1905, 1931, 2283
StyD4I CCNGG 5 cut(s) 102, 116, 1233, 1615, 2227
StyI CCWWGG 6 cut(s) 151, 211, 486, 1285, 1591, 2268
TaaI ACNGT 7 cut(s) 663, 673, 690, 793, 932, 1469, 1499
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 289, 1560, 1750, 1911
TatI WGTACW 2 cut(s) 1010, 2187
TauI GCSGC 4 cut(s) 657, 1361, 1434, 2276
TfiI GAWTC 1 cut(s) 1908
Tru1I TTAA 9 cut(s) 375, 962, 1128, 1316, 1839, 1974, 2025, 2210, 2249
Tru9I TTAA 9 cut(s) 375, 962, 1128, 1316, 1839, 1974, 2025, 2210, 2249
TscAI CASTG 4 cut(s) 18, 255, 668, 695
TseFI GTSAC 4 cut(s) 124, 504, 950, 1087
TseI GCWGC 7 cut(s) 980, 1163, 1277, 1280, 1657, 1829, 1986
Tsp45I GTSAC 4 cut(s) 124, 504, 950, 1087
TspDTI ATGAA 9 cut(s) 17, 148, 495, 909, 935, 1800, 2100, 2187, 2235
TspGWI ACGGA 1 cut(s) 1471
TspRI CASTG 4 cut(s) 18, 255, 668, 695
Vha464I CTTAAG 1 cut(s) 1127
VpaK11BI GGWCC 2 cut(s) 148, 1057
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 7 cut(s) 819, 1194, 1212, 2027, 2087, 2316, 2353
XbaI TCTAGA 2 cut(s) 1930, 2282
XceI RCATGY 6 cut(s) 132, 234, 241, 413, 1721, 2078
XcmI CCANNNNNNNNNTGG 1 cut(s) 1292
XmiI GTMKAC 2 cut(s) 927, 2239
XmnI GAANNNNTTC 2 cut(s) 78, 329
XspI CTAG 5 cut(s) 977, 1377, 1905, 1931, 2283
ZrmI AGTACT 1 cut(s) 1012
Zsp2I ATGCAT 3 cut(s) 411, 836, 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.