MD16G1224400.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000555
Physical Location & Seq
Forward (+)
22637765 .. 22657646
19882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1224400.v1.1.491

Sequence Viewer

Length: 2589 bp
ATGAGAGTGGCAGTGATTGGTGCTGGGATGAGTGGGTTGGTGTCTGCCTATGTTCTTGCAAAGGAAGGGGTGGAAGTGGTGGTGTATGAGAAGGATGACTACTTGGGCGGCCATGCCAGGACTGTCACATTTGATGGTGTTGATTTGGACCTCGGTTTCATGGTCTTCAATCGTGTAACTTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGGGTTGATATGGAGACATCTGACATGTCCTTCTCAGCGAGCTTAGACAATGGACGGGGGTGTGAATGGGGCAGCAGAAATGGTTTGTCGAGCTTGTTTGCACAAAAGACCAACTTGATCAATCCATACTTCTGGCAAATGCTTCGAGAAATAACAAAGTTTAAACATGATGCAATCAATTATCTTGAGGAGCTTGAGAACAACCAGGACATTGATCGCAGTGAAACCTTGGGGCAGTTTATCAAGTCACGCGGCTACTCTGAATTATTTCAGAAGGCTTATCTTGTTCCAGTATGTGGGTCAATCTGGTCTTGTCCTTCAGAAGGAGTTATGAGCTTCTCAGCCTTCTCTGTTCTTTCATTTTGTCGCAATCATCATCTACTTCAGCTATTTGGGCGTCCACAGTGGCTTACTGTCAGATGGCGTTCACATTGTTATGTGAAGAAGGTCAGAGAAGTTCTGGAGAGTAAAGGCTGTCACATAAGAACTAGCTCTGAAGTACATAGGGTTTCCACATCTGATGAGGGTTGCACTGTAATCTCTGGTGATGGTTTGGAGGAAGTATTCAATGGATGCATAATTGCTGTCCACCCTCCTGATGCCGTGAGAATTCTAGGTGACCAGGCCACATCGGATGAGTTGAGAGTTCTTGGTGCTTTCCAATATGTGTACAGTGACATTTTTCTGCATCGTGACAAAAAATTAATGCCCCGCAACCCAGCAGCATGGAGTGCGTGGAATTTTCTCGGAAGTAATGGCAACAAAGTTTGTTTGACATACTGGCTCAATGTGCTCCAGAACATTGATGAAACCGGTCTTCCATTTCTTGTGACCCTGAATCCAGAACACACACCAAAACATACCTTGCTTAAGTGGTCGACAAGCCATCCAGTCCCATCTGTTGCTGCATCGAAGGCTTCGCTTGAGCTTCCTCGTATTCAAGGAAAAAGAGGAATTTGGTTTTGTGGAGCATATCAGGGGTATGGCTTCCATGAGGATGGACTAAAGGCTGGAATGGATGCAGCACATGGTTTGCTTGGAAAAGGTTATGCCCTCTTGGACAACCCAAAACACATGGTTCCGTCGCTGACAGAAACAGGGGCACGCCTTTTTGTTACTAGATTTCTTACACATTTTATCTCTACCGGATGTTTGATTTTCTTGGAGGAAGGAGGAACAATTTTCACCTTTGAGGGAACCAGAAAAGGGTGTTCTCTGAAATGTGTTATTAAAGTTCATAATCCTCAGTTTTACTGGAAGGTAATGACACAGGCTGATTTAGGCCTAGCAGATGCATATATCAATGGGGATTTTTCATTCGTTGACAAAGACAAAGGTCTTTTAAATCTTATAATGATTCTCATCGCCAACAGAGACGCAAATTCTTCCGACTCAAAACTGACCAAGAAAAGGGGATGGTGGACGCCATTGTTATTCACAGCTAGTATAGCATCAGCAAAATATTTTTTCCAGCATGTTTCGAGGCAAAATACTCTCACTCAGGCTCGCAGGAACATCTCTCGTCATTATGACCTGAGTAATGAACTATTCTCGCAGTTCTTGGACGAGACAATGACGTATTCCAGTGCTGTATTTAAGACAGAAGATGAAGATTTAAAGGTTGCTCAGCTGAGGAAAATCTCCATTCTGATTGAAAAGGCTAGAATTGAAAAGAACCATGAAGTTCTAGAGATTGGATGCGGTTGGGGAAGCTTAGCTATTAAAGTCGTCAAACAAACAGGTTGCAAATACACCGGCATCACTCTGTCCGAGGAACAACTGAAATATGCACAAGAGAAAGTGAAAGAGGCTGGCCTTCAGGACCGCATCAAATTTCTTCTGTGCGATTACCGCCAGCTTGCTAGCTACAAATATGACAGAATTATATCTTGCGAGATGCTAGAAAGCGTGGGGCATGAATTTATGGACGAATTCTTTTCGTGCTGTGAATCTGTGCTAGCAGAAAATGGCCTTCTTGTTCTACAGTTCATATCAATACCAGATCAGCGCTACGATGAGTACAGGCGAAGTTCAGACTTCATAAAGGAATATATTTTTCCGGGTGGATGTCTACCTTCGTTAAGTAGGGTAACATCGGCAATGGCTGCTTCTTCCAGACTCTGTGTGGAGCACCTGGAAAACATTGGAATCCATTACTTCCAAACACTCAGATATTGGAGAAAAAATTTCTTGGACAGACAAGGTGAAATACTCGCTCTTGGATTCGATGAAAAGTTCATCCGGACATGGGAGTATTATTTTGATTATTGCGCGGCCGGTTTTAGGACGTATACTCTTGGAAATTACCAGATCGTGTTTTCACGCCCCGGCAATGTTCCGGCATTCAGCAATCCGTACAAAGGCAAGTGTTGCTGA

Protein Analysis

863

Amino Acids

97.82

Weight (kDa)

6.77

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 2.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 5.7e-18 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 277 1e-21 Flavin containing amine oxidoreductase
CMAS PF02353 570 - 839 9.2e-81 Mycolic acid cyclopropane synthetase
MTS PF05175 620 - 731 4.6e-06 Methyltransferase small domain
Methyltransf_23 PF13489 625 - 739 1.6e-08 Methyltransferase domain
Methyltransf_31 PF13847 628 - 736 1e-11 Methyltransferase domain
Methyltransf_25 PF13649 633 - 728 8.9e-15 Methyltransferase domain
Methyltransf_11 PF08241 634 - 732 5.8e-12 Methyltransferase domain
Methyltransf_12 PF08242 634 - 730 2.9e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1565
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 3 cut(s) 1091, 2284, 2504
AccII CGCG 2 cut(s) 465, 2486
AccIII TCCGGA 1 cut(s) 2454
AciI CCGC 7 cut(s) 108, 465, 925, 1914, 2038, 2065, 2486
AcoI YGGCCR 2 cut(s) 109, 2487
AcsI RAATTY 8 cut(s) 822, 952, 1167, 1594, 2045, 2132, 2144, 2398
AcuI CTGAAG 4 cut(s) 516, 581, 729, 2015
AcyI GRCGYC 2 cut(s) 610, 1637
AfaI GTAC 4 cut(s) 714, 884, 2234, 2570
AfeI AGCGCT 1 cut(s) 2222
AfiI CCNNNNNNNGG 7 cut(s) 509, 536, 1419, 1623, 2461, 2496, 2573
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 1 cut(s) 237
AgeI ACCGGT 1 cut(s) 1025
AgsI TTSAA 5 cut(s) 169, 781, 1154, 1868, 1883
AjnI CCWGG 4 cut(s) 116, 417, 834, 2346
Alw21I GWGCWC 2 cut(s) 1008, 2346
Alw26I GTCTC 3 cut(s) 221, 1581, 1775
AlwNI CAGNNNCTG 1 cut(s) 2334
Aor13HI TCCGGA 1 cut(s) 2454
Aor51HI AGCGCT 1 cut(s) 2222
AoxI GGCC 6 cut(s) 109, 837, 1495, 2026, 2182, 2487
ApeKI GCWGC 5 cut(s) 285, 935, 1118, 1235, 2318
ApoI RAATTY 8 cut(s) 822, 952, 1167, 1594, 2045, 2132, 2144, 2398
AseI ATTAAT 1 cut(s) 917
AsiGI ACCGGT 1 cut(s) 1025
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 2 cut(s) 2223, 2486
AspS9I GGNCC 2 cut(s) 148, 2035
AsuC2I CCSGG 2 cut(s) 2274, 2541
AsuHPI GGTGA 4 cut(s) 770, 842, 1390, 2429
AsuNHI GCTAGC 2 cut(s) 2075, 2170
AvaII GGWCC 2 cut(s) 148, 2035
BaeGI GKGCMC 1 cut(s) 1318
BarI GAAGNNNNNNTAC 2 cut(s) 83, 115
BbsI GAAGAC 2 cut(s) 157, 1022
Bbv12I GWGCWC 2 cut(s) 1008, 2346
BbvCI CCTCAGC 1 cut(s) 1844
BbvI GCAGC 5 cut(s) 297, 947, 1105, 1247, 2305
BccI CCATC 8 cut(s) 128, 187, 627, 755, 1107, 1117, 1205, 1623
BceAI ACGGC 1 cut(s) 800
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 4 cut(s) 118, 419, 836, 2348
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 2 cut(s) 2274, 2541
BcoDI GTCTC 3 cut(s) 221, 1581, 1775
BfmI CTRYAG 1 cut(s) 2195
BfoI RGCGCY 1 cut(s) 2224
BfrI CTTAAG 1 cut(s) 1082
BisI GCNGC 8 cut(s) 109, 286, 466, 936, 1119, 1236, 2319, 2487
BlpI GCTNAGC 2 cut(s) 1839, 1927
BlsI GCNGC 8 cut(s) 110, 287, 467, 937, 1120, 1237, 2320, 2488
Bme1390I CCNGG 6 cut(s) 118, 419, 836, 2274, 2348, 2541
Bme18I GGWCC 2 cut(s) 148, 2035
BmgT120I GGNCC 2 cut(s) 148, 2035
BmiI GGNNCC 2 cut(s) 1293, 1411
BmrFI CCNGG 6 cut(s) 118, 419, 836, 2274, 2348, 2541
BmtI GCTAGC 2 cut(s) 2079, 2174
BoxI GACNNNNGTC 1 cut(s) 1548
BpiI GAAGAC 2 cut(s) 157, 1022
BpmI CTGGAG 2 cut(s) 695, 992
Bpu10I CCTNAGC 1 cut(s) 1844
Bpu1102I GCTNAGC 2 cut(s) 1839, 1927
BpuEI CTTGAG 3 cut(s) 419, 428, 1157
BpuMI CCSGG 2 cut(s) 2274, 2541
BsaBI GATNNNNATC 1 cut(s) 1574
BsaHI GRCGYC 2 cut(s) 610, 1637
BsaJI CCNNGG 5 cut(s) 151, 211, 441, 1983, 2539
BsaWI WCCGGW 3 cut(s) 1025, 1358, 2454
Bsc4I CCNNNNNNNGG 7 cut(s) 509, 536, 1419, 1623, 2461, 2496, 2573
Bse118I RCCGGY 3 cut(s) 1025, 1967, 2489
Bse1I ACTGG 5 cut(s) 503, 998, 1103, 1472, 1797
Bse3DI GCAATG 2 cut(s) 2319, 2551
Bse8I GATNNNNATC 1 cut(s) 1574
BseAI TCCGGA 1 cut(s) 2454
BseBI CCWGG 4 cut(s) 118, 419, 836, 2348
BseDI CCNNGG 5 cut(s) 151, 211, 441, 1983, 2539
BseJI GATNNNNATC 1 cut(s) 1574
BseLI CCNNNNNNNGG 7 cut(s) 509, 536, 1419, 1623, 2461, 2496, 2573
BseMI GCAATG 2 cut(s) 2319, 2551
BseMII CTCAG 8 cut(s) 261, 567, 1472, 1727, 1739, 1835, 1853, 2395
BseNI ACTGG 5 cut(s) 503, 998, 1103, 1472, 1797
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 1318
BseX3I CGGCCG 1 cut(s) 2487
BseXI GCAGC 5 cut(s) 297, 947, 1105, 1247, 2305
BseYI CCCAGC 2 cut(s) 23, 931
Bsh1236I CGCG 2 cut(s) 465, 2486
Bsh1285I CGRYCG 1 cut(s) 2490
BshFI GGCC 6 cut(s) 111, 839, 1497, 2028, 2184, 2489
BshTI ACCGGT 1 cut(s) 1025
BsiEI CGRYCG 1 cut(s) 2490
BsiHKAI GWGCWC 2 cut(s) 1008, 2346
BsiSI CCGG 8 cut(s) 1026, 1359, 1968, 2273, 2455, 2490, 2541, 2552
BslFI GGGAC 1 cut(s) 1091
BslI CCNNNNNNNGG 7 cut(s) 509, 536, 1419, 1623, 2461, 2496, 2573
BsmAI GTCTC 3 cut(s) 221, 1581, 1775
BsmBI CGTCTC 1 cut(s) 1581
BsmFI GGGAC 1 cut(s) 1091
BsmI GAATGC 1 cut(s) 2555
BsnI GGCC 6 cut(s) 111, 839, 1497, 2028, 2184, 2489
Bsp1286I GDGCHC 3 cut(s) 1008, 1318, 2346
Bsp13I TCCGGA 1 cut(s) 2454
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 4 cut(s) 330, 427, 2215, 2523
Bsp1720I GCTNAGC 2 cut(s) 1839, 1927
BspACI CCGC 7 cut(s) 108, 465, 925, 1914, 2038, 2065, 2486
BspANI GGCC 6 cut(s) 111, 839, 1497, 2028, 2184, 2489
BspCNI CTCAG 8 cut(s) 260, 566, 1471, 1726, 1740, 1836, 1852, 2394
BspEI TCCGGA 1 cut(s) 2454
BspFNI CGCG 2 cut(s) 465, 2486
BspLI GGNNCC 2 cut(s) 1293, 1411
BspOI GCTAGC 2 cut(s) 2079, 2174
BspTI CTTAAG 1 cut(s) 1082
BsrDI GCAATG 2 cut(s) 2319, 2551
BsrFI RCCGGY 3 cut(s) 1025, 1967, 2489
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 5 cut(s) 503, 998, 1103, 1472, 1797
BssAI RCCGGY 3 cut(s) 1025, 1967, 2489
BssECI CCNNGG 5 cut(s) 151, 211, 441, 1983, 2539
BssMI GATC 4 cut(s) 330, 427, 2215, 2523
BssNAI GTATAC 1 cut(s) 2505
BssNI GRCGYC 2 cut(s) 610, 1637
BssT1I CCWWGG 2 cut(s) 211, 441
Bst1107I GTATAC 1 cut(s) 2505
Bst2UI CCWGG 4 cut(s) 118, 419, 836, 2348
Bst4CI ACNGT 6 cut(s) 124, 618, 628, 748, 887, 2199
BstACI GRCGYC 2 cut(s) 610, 1637
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 3 cut(s) 944, 2318, 2583
BstAUI TGTACA 1 cut(s) 882
BstC8I GCNNGC 8 cut(s) 253, 1318, 1720, 2026, 2069, 2073, 2077, 2172
BstEII GGTNACC 1 cut(s) 830
BstENI CCTNNNNNAGG 1 cut(s) 534
BstFNI CGCG 2 cut(s) 465, 2486
BstH2I RGCGCY 1 cut(s) 2224
BstHHI GCGC 2 cut(s) 2223, 2486
BstKTI GATC 4 cut(s) 333, 430, 2218, 2526
BstMAI GTCTC 3 cut(s) 221, 1581, 1775
BstMBI GATC 4 cut(s) 330, 427, 2215, 2523
BstMCI CGRYCG 1 cut(s) 2490
BstMWI GCNNNNNNNGC 8 cut(s) 607, 944, 1003, 1127, 1661, 2064, 2318, 2583
BstNI CCWGG 4 cut(s) 118, 419, 836, 2348
BstNSI RCATGY 2 cut(s) 241, 1691
BstPAI GACNNNNGTC 1 cut(s) 1548
BstPI GGTNACC 1 cut(s) 830
BstSCI CCNGG 6 cut(s) 116, 417, 834, 2272, 2346, 2539
BstSFI CTRYAG 1 cut(s) 2195
BstSLI GKGCMC 1 cut(s) 1318
BstUI CGCG 2 cut(s) 465, 2486
BstV1I GCAGC 5 cut(s) 297, 947, 1105, 1247, 2305
BstV2I GAAGAC 2 cut(s) 157, 1022
BstXI CCANNNNNNTGG 3 cut(s) 345, 939, 1211
BstZ17I GTATAC 1 cut(s) 2505
BstZI CGGCCG 1 cut(s) 2487
BsuRI GGCC 6 cut(s) 111, 839, 1497, 2028, 2184, 2489
BtgZI GCGATG 1 cut(s) 1561
BtsI GCAGTG 2 cut(s) 18, 439
BtsIMutI CAGTG 6 cut(s) 18, 439, 623, 744, 892, 1804
Cac8I GCNNGC 8 cut(s) 253, 1318, 1720, 2026, 2069, 2073, 2077, 2172
CaiI CAGNNNCTG 1 cut(s) 2334
CfoI GCGC 2 cut(s) 2223, 2486
Cfr10I RCCGGY 3 cut(s) 1025, 1967, 2489
Cfr13I GGNCC 2 cut(s) 148, 2035
CseI GACGC 3 cut(s) 599, 1598, 1645
Csp6I GTAC 4 cut(s) 713, 883, 2233, 2569
CspAI ACCGGT 1 cut(s) 1025
CviQI GTAC 4 cut(s) 713, 883, 2233, 2569
DpnI GATC 4 cut(s) 332, 429, 2217, 2525
DpnII GATC 4 cut(s) 330, 427, 2215, 2523
DraI TTTAAA 3 cut(s) 376, 1557, 1830
EaeI YGGCCR 2 cut(s) 109, 2487
EagI CGGCCG 1 cut(s) 2487
EclXI CGGCCG 1 cut(s) 2487
Eco130I CCWWGG 2 cut(s) 211, 441
Eco147I AGGCCT 1 cut(s) 1497
Eco47I GGWCC 2 cut(s) 148, 2035
Eco47III AGCGCT 1 cut(s) 2222
Eco52I CGGCCG 1 cut(s) 2487
Eco57I CTGAAG 4 cut(s) 516, 581, 729, 2015
Eco91I GGTNACC 1 cut(s) 830
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoO65I GGTNACC 1 cut(s) 830
EcoRI GAATTC 2 cut(s) 822, 2144
EcoRII CCWGG 4 cut(s) 116, 417, 834, 2346
EcoT14I CCWWGG 2 cut(s) 211, 441
EcoT22I ATGCAT 2 cut(s) 791, 1510
ErhI CCWWGG 2 cut(s) 211, 441
Esp3I CGTCTC 1 cut(s) 1581
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FaqI GGGAC 1 cut(s) 1091
FauI CCCGC 1 cut(s) 932
FbaI TGATCA 1 cut(s) 330
FblI GTMKAC 3 cut(s) 1091, 2284, 2504
Fnu4HI GCNGC 8 cut(s) 109, 286, 466, 936, 1119, 1236, 2319, 2487
Fsp4HI GCNGC 8 cut(s) 109, 286, 466, 936, 1119, 1236, 2319, 2487
GlaI GCGC 2 cut(s) 2222, 2485
GluI GCNGC 8 cut(s) 109, 286, 466, 936, 1119, 1236, 2319, 2487
GsaI CCCAGC 2 cut(s) 27, 935
GsuI CTGGAG 2 cut(s) 695, 992
HaeII RGCGCY 1 cut(s) 2224
HaeIII GGCC 6 cut(s) 111, 839, 1497, 2028, 2184, 2489
HapII CCGG 8 cut(s) 1026, 1359, 1968, 2273, 2455, 2490, 2541, 2552
HgaI GACGC 3 cut(s) 599, 1598, 1645
HhaI GCGC 2 cut(s) 2223, 2486
Hin1I GRCGYC 2 cut(s) 610, 1637
Hin6I GCGC 2 cut(s) 2221, 2484
HinP1I GCGC 2 cut(s) 2221, 2484
HincII GTYRAC 2 cut(s) 1092, 1537
HindII GTYRAC 2 cut(s) 1092, 1537
HindIII AAGCTT 1 cut(s) 1924
HinfI GANTC 7 cut(s) 1051, 1570, 1604, 2162, 2331, 2361, 2436
HpaII CCGG 8 cut(s) 1026, 1359, 1968, 2273, 2455, 2490, 2541, 2552
HphI GGTGA 4 cut(s) 770, 842, 1390, 2429
Hpy166II GTNNAC 9 cut(s) 614, 641, 802, 883, 1092, 1537, 1635, 2285, 2505
Hpy8I GTNNAC 9 cut(s) 614, 641, 802, 883, 1092, 1537, 1635, 2285, 2505
Hpy99I CGWCG 1 cut(s) 1300
HpyCH4III ACNGT 6 cut(s) 124, 618, 628, 748, 887, 2199
HpyCH4IV ACGT 2 cut(s) 1790, 2501
HpyF10VI GCNNNNNNNGC 8 cut(s) 607, 944, 1003, 1127, 1661, 2064, 2318, 2583
HpySE526I ACGT 2 cut(s) 1790, 2501
Hsp92I GRCGYC 2 cut(s) 610, 1637
HspAI GCGC 2 cut(s) 2221, 2484
Kpn2I TCCGGA 1 cut(s) 2454
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 4 cut(s) 330, 427, 2215, 2523
LmnI GCTCC 4 cut(s) 403, 1011, 1181, 2341
Lsp1109I GCAGC 5 cut(s) 297, 947, 1105, 1247, 2305
MaeII ACGT 2 cut(s) 1790, 2501
MalI GATC 4 cut(s) 332, 429, 2217, 2525
MboI GATC 4 cut(s) 330, 427, 2215, 2523
MboII GAAGA 8 cut(s) 157, 667, 1022, 1590, 1829, 1835, 2042, 2316
MhlI GDGCHC 3 cut(s) 1008, 1318, 2346
MlyI GAGTC 2 cut(s) 1598, 2325
MmeI TCCRAC 1 cut(s) 1626
Mph1103I ATGCAT 2 cut(s) 791, 1510
MroI TCCGGA 1 cut(s) 2454
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 9 cut(s) 375, 917, 1083, 1443, 1556, 1809, 1829, 1935, 2294
MslI CAYNNNNRTG 2 cut(s) 648, 1209
MspA1I CMGCKG 1 cut(s) 1843
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 8 cut(s) 1026, 1359, 1968, 2273, 2455, 2490, 2541, 2552
MspR9I CCNGG 6 cut(s) 118, 419, 836, 2274, 2348, 2541
MssI GTTTAAAC 1 cut(s) 376
Mva1269I GAATGC 1 cut(s) 2555
MvaI CCWGG 4 cut(s) 118, 419, 836, 2348
MvnI CGCG 2 cut(s) 465, 2486
MwoI GCNNNNNNNGC 8 cut(s) 607, 944, 1003, 1127, 1661, 2064, 2318, 2583
NciI CCSGG 2 cut(s) 2274, 2541
NdeII GATC 4 cut(s) 330, 427, 2215, 2523
NheI GCTAGC 2 cut(s) 2075, 2170
NlaIV GGNNCC 2 cut(s) 1293, 1411
NmuCI GTSAC 7 cut(s) 124, 459, 689, 830, 887, 905, 1042
NsiI ATGCAT 2 cut(s) 791, 1510
NspI RCATGY 2 cut(s) 241, 1691
PceI AGGCCT 1 cut(s) 1497
PciI ACATGT 1 cut(s) 237
PctI GAATGC 1 cut(s) 2555
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 5 cut(s) 1051, 1570, 2162, 2361, 2436
PflMI CCANNNNNTGG 1 cut(s) 509
PinAI ACCGGT 1 cut(s) 1025
PkrI GCNGC 8 cut(s) 110, 287, 467, 937, 1120, 1237, 2320, 2488
PleI GAGTC 2 cut(s) 1598, 2325
PmeI GTTTAAAC 1 cut(s) 376
PpsI GAGTC 2 cut(s) 1598, 2325
PscI ACATGT 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 1548
PshBI ATTAAT 1 cut(s) 917
PsiI TTATAA 1 cut(s) 1565
Psp6I CCWGG 4 cut(s) 116, 417, 834, 2346
PspEI GGTNACC 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 23, 931
PspGI CCWGG 4 cut(s) 116, 417, 834, 2346
PspN4I GGNNCC 2 cut(s) 1293, 1411
PspPI GGNCC 2 cut(s) 148, 2035
PstNI CAGNNNCTG 1 cut(s) 2334
PvuII CAGCTG 1 cut(s) 1843
RsaI GTAC 4 cut(s) 714, 884, 2234, 2570
RsaNI GTAC 4 cut(s) 713, 883, 2233, 2569
RseI CAYNNNNRTG 2 cut(s) 648, 1209
SalI GTCGAC 1 cut(s) 1090
SaqAI TTAA 9 cut(s) 375, 917, 1083, 1443, 1556, 1809, 1829, 1935, 2294
SatI GCNGC 8 cut(s) 109, 286, 466, 936, 1119, 1236, 2319, 2487
Sau3AI GATC 4 cut(s) 330, 427, 2215, 2523
Sau96I GGNCC 2 cut(s) 148, 2035
SchI GAGTC 2 cut(s) 1598, 2325
ScrFI CCNGG 6 cut(s) 118, 419, 836, 2274, 2348, 2541
SduI GDGCHC 3 cut(s) 1008, 1318, 2346
SfcI CTRYAG 1 cut(s) 2195
SinI GGWCC 2 cut(s) 148, 2035
SmiMI CAYNNNNRTG 2 cut(s) 648, 1209
SmlI CTYRAG 4 cut(s) 398, 407, 1082, 1136
SmoI CTYRAG 4 cut(s) 398, 407, 1082, 1136
SseBI AGGCCT 1 cut(s) 1497
SsiI CCGC 7 cut(s) 108, 465, 925, 1914, 2038, 2065, 2486
SspI AATATT 1 cut(s) 1676
StuI AGGCCT 1 cut(s) 1497
StyD4I CCNGG 6 cut(s) 116, 417, 834, 2272, 2346, 2539
StyI CCWWGG 2 cut(s) 211, 441
TaaI ACNGT 6 cut(s) 124, 618, 628, 748, 887, 2199
TaiI ACGT 2 cut(s) 1793, 2504
TaqI TCGA 6 cut(s) 302, 358, 1091, 1124, 1694, 2439
TatI WGTACW 3 cut(s) 712, 882, 2232
TauI GCSGC 3 cut(s) 111, 468, 2489
TfiI GAWTC 5 cut(s) 1051, 1570, 2162, 2361, 2436
Tru1I TTAA 9 cut(s) 375, 917, 1083, 1443, 1556, 1809, 1829, 1935, 2294
Tru9I TTAA 9 cut(s) 375, 917, 1083, 1443, 1556, 1809, 1829, 1935, 2294
TscAI CASTG 6 cut(s) 18, 439, 623, 751, 892, 1804
TseFI GTSAC 7 cut(s) 124, 459, 689, 830, 887, 905, 1042
TseI GCWGC 5 cut(s) 285, 935, 1118, 1235, 2318
Tsp45I GTSAC 7 cut(s) 124, 459, 689, 830, 887, 905, 1042
TspGWI ACGGA 2 cut(s) 1284, 2556
TspRI CASTG 6 cut(s) 18, 439, 623, 751, 892, 1804
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 2 cut(s) 148, 2035
VspI ATTAAT 1 cut(s) 917
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 8 cut(s) 822, 952, 1167, 1594, 2045, 2132, 2144, 2398
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 2 cut(s) 241, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 2335
XmiI GTMKAC 3 cut(s) 1091, 2284, 2504
XmnI GAANNNNTTC 1 cut(s) 480
Zsp2I ATGCAT 2 cut(s) 791, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.