Rw4G004380

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000555
Physical Location & Seq
Forward (+)
8697377 .. 8709734
12358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004380.1

Sequence Viewer

Length: 2532 bp
ATGAGAGTGGCAGTGATTGGTTCTGGTATCAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGATGGGGTTGAAGTGGATCTGTATGAGAAAGAAGACTACTTGGGTGGTCATGCCAGGACGGTCACATTTGATGGCGTCGATTTGGACCTTGGTTTCATGGTCTTCAATCGAGTAACATATCCCAACATGATGGAGTTCTTTGAGAGTCTTGGAGTTGAGATGGAGATATCTGACATGTCCTTCTCGGCAAGCTTAGACAAAGGAAAAGGCTGTGAATGGGGTAGTCGAAATGGTCTGTCGAGCTTGTTTGCGCAAAAGAGCAACGTGTTAAATCCATACTTTTGGCAAATGCTTCGAGAAATCACCAAGTTTAAGCATGATGCCATCAGTTATCTTGAGGTGCATGAGAACAATCCGGACATTGATCGAAATGAAACCTTGGGGAAATTTATCGAGTCCCGTCACTACTCCGAATTATTTCAGAAGGCTTATCTTGTTCCAATATGTGGTTCAATCTGGTCATGCCCTGCAGAAGGGGTTATGAGCTTCTCAGCCTTCTCCGTTCTTTCGTTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCCGCCCACAATGGTTAACTGTCAGATGGCGTTCACATTGTTATGTGAAGAAGGTTAGAGAAGTGCTGGAGAGTAAAGGGTGTCGGATAAGAACAGGTTGTGAGGTGCATAAAGTTTCCACAACCGATGAGGGTGAAATATTAGGAGATCAGGCAACATCAGATGAACTGAGAGTACTTGGTGCCTTCCAATATGTATACAGTGATATTTTCCTCCATCGTGACAAAGATTTAATGCCCCAGAACCCGGCAGCATGGAGTGCATGGAATTTTCTCGGTAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATGTGCTTCAGAACCTTGGTGAAACAAGTCTACCATTTCTTGTGACTCTCAATCCAGATCATACACCAAAACATACCTTGCTTAAGTGGTCAACAAGCCATCCAATCCCATCTGTTGCTGCATCAAAGGCTACACTTGAGCTTCCTCATATTCAAGGAAAGAGAGGAATCTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGATTAAAGGCTGGAATGACTGTAGCTCATGGTATTCTTGGAAACAGTTGTGCCCTCTTGAGCAACCCCAAACACATGGTTCCATCATTGACAGAAAGTGGGGCACGCCTTTTTGTAACTAGATTTATAAGACATTATATCTCTACTGGATGTTTAATGAACAAGAAAAGGGTTTCTTTAAAATGTGTTCTTAAAGTTCATAATCCTCAGTTTTACTGGAAGGTAATGACACAGGCTGATCTAGGCCTTGCAGATGCATATATCAACGGAGATTTCTCTTTTGTTGACAAAGACAAAGGCCTCCTAAATCTTTTCATGATTCTCATTGCCAACAGAGATAATGATTCTTCTGACTCAAAATTGATTAAGAAAAGGGGATGGTGGACTCCAATGTTGTTCACAGCTAGTATAGCCTCAGCAAAATATTTCTTTCAGCATGTTTCGAGGCAAAATACTCTAACACAGGCTCGGAGGAACATCTCTCGTCATTATGACCTGAGTAATGAACTATTTTCGCTGTTCTTGGACGAAACAATGACGTATTCTAGTGCTGTATTTAAGACAGAAGATGAGGAGCTGAAGATTGCACAGCTAAGGAAAATATCTATTCTCATTGAAAAGGCTAAAATTAGTAAGAACCATGAAGTTCTAGAGATTGGATGTGGTTGGGGAAGCTTGGCTATCGAAGTTGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAGTTTGCACAACAGAAAGTGAAAGATGCTGGCATTCAGGACCGGATCAGATTTCTTTTATGTGACTACCGCCAATTGCCTTCTAACTACAAATGTGACAGAATCATATCGTGCGAGATGCTAGAGAGTGTTGGCCACGAATTTATGAATGATTTCTTTGCTTCCTGTGAATCTGTGCTAGCAGCAAATGGGCTTCTTGTTCTGCAGTTCATATCAATACCGGATGAACGCTATGACGAGTACAGACGAAGTTCAGACTTCATAAAGGAGTACATTTTCCCTGGTGGTTGTCTACCTTCATTGAGTAGAGTAACATCAGCCATGGTTGGTTCTTCTAGGCTCTGTGTGGAGCACTTGGAAAATATCGGAATTCATTACTACCAAACACTGAGATATTGGAGAAAAAATTTCTTGGAGAGACAAAGTGAAATACTAGCTCTTGGATTCAACGAGAAATTTATTCGGACATGGGAATACTATTTTGATTATTGTGCTGCCGGTTTCAAGACATATACGCTTGGAAACTACCAGATTGTGTTTTCACGTCCTGGCAATGTTCCAGCATTCAGCAATCCCTACAAAGGACTAAATATCATATTCCTATGGTTCGAGATTTTGCACAATACCTATTTTCTGCTGTGTTTGATATCATGA

Protein Analysis

843

Amino Acids

96.31

Weight (kDa)

7.52

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 2.4e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 1.3e-17 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 247 1.6e-16 Flavin containing amine oxidoreductase
CMAS PF02353 531 - 800 3e-79 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 587 - 701 1.5e-06 Methyltransferase domain
Methyltransf_31 PF13847 589 - 698 7.6e-11 Methyltransferase domain
Methyltransf_25 PF13649 594 - 690 3.1e-15 Methyltransferase domain
Methyltransf_11 PF08241 595 - 694 1.6e-12 Methyltransferase domain
Methyltransf_12 PF08242 595 - 692 1.2e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1262
Acc16I TGCGCA 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 788
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 3 cut(s) 804, 952, 2170
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 2 cut(s) 610, 1948
AclWI GGATC 2 cut(s) 87, 1931
AcoI YGGCCR 2 cut(s) 607, 2011
AcsI RAATTY 6 cut(s) 449, 874, 2018, 2247, 2284, 2333
AcuI CTGAAG 3 cut(s) 581, 914, 1733
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 4 cut(s) 783, 889, 2120, 2150
AfiI CCNNNNNNNGG 4 cut(s) 509, 536, 853, 2459
AflII CTTAAG 2 cut(s) 1004, 1877
AflIII ACRYGT 2 cut(s) 237, 327
AgsI TTSAA 7 cut(s) 74, 169, 516, 1076, 1751, 2326, 2383
AjiI CACGTC 1 cut(s) 2423
AjnI CCWGG 4 cut(s) 116, 1110, 2158, 2425
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
Alw21I GWGCWC 1 cut(s) 2232
Alw26I GTCTC 2 cut(s) 46, 2290
AlwI GGATC 2 cut(s) 87, 1931
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 4 cut(s) 607, 1378, 1432, 2011
ApeKI GCWGC 4 cut(s) 857, 1040, 2060, 2372
ApoI RAATTY 6 cut(s) 449, 874, 2018, 2247, 2284, 2333
Asp700I GAANNNNTTC 2 cut(s) 480, 2030
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 148, 1918
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 3 cut(s) 358, 752, 953
AsuNHI GCTAGC 1 cut(s) 2056
AvaII GGWCC 2 cut(s) 148, 1918
BaeGI GKGCMC 2 cut(s) 1189, 1240
BalI TGGCCA 1 cut(s) 2013
BanI GGYRCC 1 cut(s) 788
BbsI GAAGAC 2 cut(s) 102, 157
Bbv12I GWGCWC 1 cut(s) 2232
BbvCI CCTCAGC 1 cut(s) 1549
BbvI GCAGC 4 cut(s) 869, 1027, 2072, 2359
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 4 cut(s) 118, 1112, 2160, 2427
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 2 cut(s) 46, 2290
BfaI CTAG 9 cut(s) 1254, 1376, 1539, 1680, 1784, 2000, 2057, 2214, 2312
BfmI CTRYAG 3 cut(s) 531, 1155, 2081
BfrI CTTAAG 2 cut(s) 1004, 1877
BisI GCNGC 5 cut(s) 610, 858, 1041, 2061, 2373
BlsI GCNGC 5 cut(s) 611, 859, 1042, 2062, 2374
BmcAI AGTACT 2 cut(s) 783, 889
Bme1390I CCNGG 5 cut(s) 118, 854, 1112, 2160, 2427
Bme18I GGWCC 2 cut(s) 148, 1918
BmgBI CACGTC 1 cut(s) 2423
BmgT120I GGNCC 2 cut(s) 148, 1918
BmiI GGNNCC 2 cut(s) 790, 1215
BmrFI CCNGG 5 cut(s) 118, 854, 1112, 2160, 2427
BmsI GCATC 6 cut(s) 373, 1052, 1378, 1863, 1894, 1986
BmtI GCTAGC 1 cut(s) 2060
BpiI GAAGAC 2 cut(s) 102, 157
BpmI CTGGAG 1 cut(s) 695
Bpu10I CCTNAGC 2 cut(s) 1549, 1727
BpuEI CTTGAG 3 cut(s) 419, 1079, 1213
BpuMI CCSGG 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 6 cut(s) 151, 441, 937, 1111, 2158, 2199
BsaWI WCCGGW 3 cut(s) 418, 1920, 2098
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 536, 853, 2459
Bse118I RCCGGY 1 cut(s) 2375
Bse1I ACTGG 4 cut(s) 920, 1285, 1355, 1855
Bse3DI GCAATG 2 cut(s) 1458, 2437
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 4 cut(s) 118, 1112, 2160, 2427
BseDI CCNNGG 6 cut(s) 151, 441, 937, 1111, 2158, 2199
BseGI GGATG 6 cut(s) 1021, 1138, 1289, 1517, 1799, 2107
BseLI CCNNNNNNNGG 4 cut(s) 509, 536, 853, 2459
BseMI GCAATG 2 cut(s) 1458, 2437
BseMII CTCAG 8 cut(s) 57, 567, 767, 1355, 1563, 1622, 1857, 2258
BseNI ACTGG 4 cut(s) 920, 1285, 1355, 1855
BseRI GAGGAG 1 cut(s) 1721
BseSI GKGCMC 2 cut(s) 1189, 1240
BseXI GCAGC 4 cut(s) 869, 1027, 2072, 2359
BshFI GGCC 4 cut(s) 609, 1380, 1434, 2013
BshNI GGYRCC 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 2232
BsiSI CCGG 5 cut(s) 419, 854, 1921, 2099, 2376
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 4 cut(s) 509, 536, 853, 2459
BsmAI GTCTC 2 cut(s) 46, 2290
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 1911, 2441
BsnI GGCC 4 cut(s) 609, 1380, 1434, 2013
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 3 cut(s) 1189, 1240, 2232
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
Bsp19I CCATGG 1 cut(s) 2199
BspACI CCGC 2 cut(s) 610, 1948
BspANI GGCC 4 cut(s) 609, 1380, 1434, 2013
BspCNI CTCAG 8 cut(s) 56, 566, 768, 1354, 1562, 1623, 1858, 2259
BspEI TCCGGA 1 cut(s) 418
BspHI TCATGA 2 cut(s) 1449, 2528
BspLI GGNNCC 2 cut(s) 790, 1215
BspMAI CTGCAG 2 cut(s) 535, 2085
BspOI GCTAGC 1 cut(s) 2060
BspPI GGATC 2 cut(s) 87, 1931
BspT107I GGYRCC 1 cut(s) 788
BspTI CTTAAG 2 cut(s) 1004, 1877
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 2 cut(s) 1458, 2437
BsrFI RCCGGY 1 cut(s) 2375
BsrI ACTGG 4 cut(s) 920, 1285, 1355, 1855
BssAI RCCGGY 1 cut(s) 2375
BssECI CCNNGG 6 cut(s) 151, 441, 937, 1111, 2158, 2199
BssMI GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
BssNAI GTATAC 1 cut(s) 805
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 4 cut(s) 151, 441, 937, 2199
Bst1107I GTATAC 1 cut(s) 805
Bst2UI CCWGG 4 cut(s) 118, 1112, 2160, 2427
Bst4CI ACNGT 5 cut(s) 124, 628, 809, 1156, 1181
BstACI GRCGYC 1 cut(s) 138
BstAFI CTTAAG 2 cut(s) 1004, 1877
BstAPI GCANNNNNTGC 1 cut(s) 866
BstC8I GCNNGC 4 cut(s) 253, 1240, 1909, 2058
BstDSI CCRYGG 1 cut(s) 2199
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 6 cut(s) 1021, 1138, 1289, 1517, 1799, 2107
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 6 cut(s) 82, 430, 757, 982, 1375, 1926
BstMAI GTCTC 2 cut(s) 46, 2290
BstMBI GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
BstMWI GCNNNNNNNGC 4 cut(s) 866, 925, 1049, 1544
BstNI CCWGG 4 cut(s) 118, 1112, 2160, 2427
BstNSI RCATGY 2 cut(s) 241, 1574
BstSCI CCNGG 5 cut(s) 116, 852, 1110, 2158, 2425
BstSFI CTRYAG 3 cut(s) 531, 1155, 2081
BstSLI GKGCMC 2 cut(s) 1189, 1240
BstV1I GCAGC 4 cut(s) 869, 1027, 2072, 2359
BstV2I GAAGAC 2 cut(s) 102, 157
BstX2I RGATCY 1 cut(s) 79
BstXI CCANNNNNNTGG 5 cut(s) 193, 345, 1118, 1133, 1210
BstYI RGATCY 1 cut(s) 79
BstZ17I GTATAC 1 cut(s) 805
BsuRI GGCC 4 cut(s) 609, 1380, 1434, 2013
BtgI CCRYGG 1 cut(s) 2199
BtrI CACGTC 1 cut(s) 2423
BtsCI GGATG 6 cut(s) 1021, 1138, 1289, 1517, 1799, 2107
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 5 cut(s) 18, 37, 814, 1848, 2264
Cac8I GCNNGC 4 cut(s) 253, 1240, 1909, 2058
CciI TCATGA 2 cut(s) 1449, 2528
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 2375
Cfr13I GGNCC 2 cut(s) 148, 1918
CseI GACGC 1 cut(s) 127
Csp6I GTAC 4 cut(s) 782, 888, 2119, 2149
CviQI GTAC 4 cut(s) 782, 888, 2119, 2149
DpnI GATC 6 cut(s) 81, 429, 756, 981, 1374, 1925
DpnII GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
DraI TTTAAA 1 cut(s) 1314
EaeI YGGCCR 2 cut(s) 607, 2011
Eco130I CCWWGG 4 cut(s) 151, 441, 937, 2199
Eco147I AGGCCT 2 cut(s) 1380, 1434
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 2 cut(s) 231, 2526
Eco47I GGWCC 2 cut(s) 148, 1918
Eco57I CTGAAG 3 cut(s) 581, 914, 1733
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 2247
EcoRII CCWGG 4 cut(s) 116, 1110, 2158, 2425
EcoRV GATATC 2 cut(s) 231, 2526
EcoT14I CCWWGG 4 cut(s) 151, 441, 937, 2199
EcoT22I ATGCAT 1 cut(s) 1393
ErhI CCWWGG 4 cut(s) 151, 441, 937, 2199
FalI AAGNNNNNCTT 2 cut(s) 1294, 1326
FaqI GGGAC 1 cut(s) 445
FblI GTMKAC 3 cut(s) 804, 952, 2170
Fnu4HI GCNGC 5 cut(s) 610, 858, 1041, 2061, 2373
FokI GGATG 6 cut(s) 1008, 1145, 1296, 1524, 1806, 2114
Fsp4HI GCNGC 5 cut(s) 610, 858, 1041, 2061, 2373
FspBI CTAG 9 cut(s) 1254, 1376, 1539, 1680, 1784, 2000, 2057, 2214, 2312
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 1 cut(s) 315
GluI GCNGC 5 cut(s) 610, 858, 1041, 2061, 2373
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 4 cut(s) 609, 1380, 1434, 2013
HapII CCGG 5 cut(s) 419, 854, 1921, 2099, 2376
HgaI GACGC 1 cut(s) 127
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 3 cut(s) 624, 1014, 1420
HindII GTYRAC 3 cut(s) 624, 1014, 1420
HindIII AAGCTT 2 cut(s) 253, 1807
HpaI GTTAAC 1 cut(s) 624
HpaII CCGG 5 cut(s) 419, 854, 1921, 2099, 2376
HphI GGTGA 3 cut(s) 358, 752, 953
Hpy166II GTNNAC 9 cut(s) 624, 641, 805, 953, 1014, 1420, 1518, 1533, 2171
Hpy8I GTNNAC 9 cut(s) 624, 641, 805, 953, 1014, 1420, 1518, 1533, 2171
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 9 cut(s) 253, 481, 530, 568, 652, 802, 1348, 1968, 2184
HpyCH4III ACNGT 5 cut(s) 124, 628, 809, 1156, 1181
HpyCH4IV ACGT 3 cut(s) 327, 1673, 2422
HpyF10VI GCNNNNNNNGC 4 cut(s) 866, 925, 1049, 1544
HpySE526I ACGT 3 cut(s) 327, 1673, 2422
Hsp92I GRCGYC 1 cut(s) 138
HspAI GCGC 1 cut(s) 314
Kpn2I TCCGGA 1 cut(s) 418
KspAI GTTAAC 1 cut(s) 624
Kzo9I GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
LmnI GCTCC 3 cut(s) 1103, 1708, 2227
Lsp1109I GCAGC 4 cut(s) 869, 1027, 2072, 2359
LweI GCATC 6 cut(s) 373, 1052, 1378, 1863, 1894, 1986
MaeI CTAG 9 cut(s) 1254, 1376, 1539, 1680, 1784, 2000, 2057, 2214, 2312
MaeII ACGT 3 cut(s) 327, 1673, 2422
MaeIII GTNAC 9 cut(s) 124, 175, 464, 827, 964, 1249, 1940, 1973, 2188
MalI GATC 6 cut(s) 81, 429, 756, 981, 1374, 1925
MboI GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
MboII GAAGA 7 cut(s) 107, 157, 667, 1473, 1712, 1726, 2202
MfeI CAATTG 1 cut(s) 1952
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 3 cut(s) 1189, 1240, 2232
MlsI TGGCCA 1 cut(s) 2013
MluNI TGGCCA 1 cut(s) 2013
MlyI GAGTC 5 cut(s) 217, 467, 961, 1481, 1513
MmeI TCCRAC 1 cut(s) 671
Mox20I TGGCCA 1 cut(s) 2013
Mph1103I ATGCAT 1 cut(s) 1393
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 2 cut(s) 480, 2030
MscI TGGCCA 1 cut(s) 2013
MslI CAYNNNNRTG 2 cut(s) 648, 1131
Msp20I TGGCCA 1 cut(s) 2013
MspCI CTTAAG 2 cut(s) 1004, 1877
MspI CCGG 5 cut(s) 419, 854, 1921, 2099, 2376
MspR9I CCNGG 5 cut(s) 118, 854, 1112, 2160, 2427
MunI CAATTG 1 cut(s) 1952
Mva1269I GAATGC 2 cut(s) 1911, 2441
MvaI CCWGG 4 cut(s) 118, 1112, 2160, 2427
MwoI GCNNNNNNNGC 4 cut(s) 866, 925, 1049, 1544
NciI CCSGG 1 cut(s) 854
NcoI CCATGG 1 cut(s) 2199
NdeII GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
NheI GCTAGC 1 cut(s) 2056
NlaIV GGNNCC 2 cut(s) 790, 1215
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 6 cut(s) 124, 464, 827, 964, 1940, 1973
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 1393
NspI RCATGY 2 cut(s) 241, 1574
PagI TCATGA 2 cut(s) 1449, 2528
PceI AGGCCT 2 cut(s) 1380, 1434
PciI ACATGT 1 cut(s) 237
PctI GAATGC 2 cut(s) 1911, 2441
PdmI GAANNNNTTC 2 cut(s) 480, 2030
PfeI GAWTC 6 cut(s) 1089, 1453, 1478, 1980, 2048, 2322
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 5 cut(s) 611, 859, 1042, 2062, 2374
PleI GAGTC 5 cut(s) 216, 466, 961, 1481, 1513
PpsI GAGTC 5 cut(s) 216, 466, 961, 1481, 1513
PscI ACATGT 1 cut(s) 237
PsiI TTATAA 1 cut(s) 1262
Psp6I CCWGG 4 cut(s) 116, 1110, 2158, 2425
PspGI CCWGG 4 cut(s) 116, 1110, 2158, 2425
PspN4I GGNNCC 2 cut(s) 790, 1215
PspPI GGNCC 2 cut(s) 148, 1918
PsrI GAACNNNNNNTAC 2 cut(s) 765, 797
PstI CTGCAG 2 cut(s) 535, 2085
PsuI RGATCY 1 cut(s) 79
RsaI GTAC 4 cut(s) 783, 889, 2120, 2150
RsaNI GTAC 4 cut(s) 782, 888, 2119, 2149
RseI CAYNNNNRTG 2 cut(s) 648, 1131
SatI GCNGC 5 cut(s) 610, 858, 1041, 2061, 2373
Sau3AI GATC 6 cut(s) 79, 427, 754, 979, 1372, 1923
Sau96I GGNCC 2 cut(s) 148, 1918
ScaI AGTACT 2 cut(s) 783, 889
SchI GAGTC 5 cut(s) 217, 467, 961, 1481, 1513
ScrFI CCNGG 5 cut(s) 118, 854, 1112, 2160, 2427
SduI GDGCHC 3 cut(s) 1189, 1240, 2232
SfaNI GCATC 6 cut(s) 373, 1052, 1378, 1863, 1894, 1986
SfcI CTRYAG 3 cut(s) 531, 1155, 2081
SinI GGWCC 2 cut(s) 148, 1918
SmiMI CAYNNNNRTG 2 cut(s) 648, 1131
SmlI CTYRAG 5 cut(s) 398, 1004, 1058, 1192, 1877
SmoI CTYRAG 5 cut(s) 398, 1004, 1058, 1192, 1877
SseBI AGGCCT 2 cut(s) 1380, 1434
SsiI CCGC 2 cut(s) 610, 1948
SspI AATATT 2 cut(s) 747, 1559
SspMI CTAG 9 cut(s) 1254, 1376, 1539, 1680, 1784, 2000, 2057, 2214, 2312
StuI AGGCCT 2 cut(s) 1380, 1434
StyD4I CCNGG 5 cut(s) 116, 852, 1110, 2158, 2425
StyI CCWWGG 4 cut(s) 151, 441, 937, 2199
TaaI ACNGT 5 cut(s) 124, 628, 809, 1156, 1181
TaiI ACGT 3 cut(s) 330, 1676, 2425
TatI WGTACW 4 cut(s) 781, 887, 2118, 2148
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 6 cut(s) 1089, 1453, 1478, 1980, 2048, 2322
TscAI CASTG 5 cut(s) 18, 37, 814, 1855, 2271
TseFI GTSAC 6 cut(s) 124, 464, 827, 964, 1940, 1973
TseI GCWGC 4 cut(s) 857, 1040, 2060, 2372
Tsp45I GTSAC 6 cut(s) 124, 464, 827, 964, 1940, 1973
TspGWI ACGGA 2 cut(s) 553, 1416
TspRI CASTG 5 cut(s) 18, 37, 814, 1855, 2271
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 2 cut(s) 1004, 1877
VpaK11BI GGWCC 2 cut(s) 148, 1918
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 6 cut(s) 449, 874, 2018, 2247, 2284, 2333
XbaI TCTAGA 1 cut(s) 1783
XceI RCATGY 2 cut(s) 241, 1574
XmiI GTMKAC 3 cut(s) 804, 952, 2170
XmnI GAANNNNTTC 2 cut(s) 480, 2030
XspI CTAG 9 cut(s) 1254, 1376, 1539, 1680, 1784, 2000, 2057, 2214, 2312
ZrmI AGTACT 2 cut(s) 783, 889
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.