Rh1AG090300

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000555
Physical Location & Seq
Forward (+)
15535445 .. 15546928
11484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG090300.1

Sequence Viewer

Length: 1332 bp
ATGGCTCTGTTGGGAAACCCAAAACATATGGCTCTTTCATTGACGGAAACAGCGGCACGCCTATTTGTTACTAGATTTCTTGGACATTATATATCTACTGGATGTTTAATCTTAATAGAAGATGGAGGCACAAGGTTCATCTTTGAGGGAAGCAGAAATGAGTGTCCTCTAAAATCCGTTCTTAAAGTTCATAGTCCTCAGTTTTACTGGAAAGTAATGACAGAGGCTACTCTAGGCATTGCAGATGCATATATCAATGGTGATTTTTCTTTCAATGATAAAGATCGAGGTCTTCTAAATCTTTTTACGCTTCTCATTGCCAATAGAGATTCTAATGCCTCAAAATCGAACAAGAAAAGGGGATGGTGGCAACCTTTGTTTTTCACAGCCACTATAGTCTCAGCCAAATATTTCTTTCAACATGCTTTAAGGCAAAATAGTCTTACACAAGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCACTATTCTTGGGCGAAACAATGATGTATTCCACTGCAATATTTCAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTGTACTCTCATTGATAAGGCTAGAATTGATGAGAACCATGAGGTTCTGGATATTGGATGTGGTTGGGGTACCTTAGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATTACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTTTGACCACAAATATGACAGAATCATTTCTTGCGAGATGCTAGAACATGTCGGCCATGAATTTGTGGATGAGTTTTTTTCATGCTGTGAGTCTTTACTAGCAGAAAATGGGATTCTTGTTCTGCAGTTCATATCTATACCGGATGAACGCTATGATGAGTTTAGGCAAAGTTCAAGCTTCTTAAATGAATATATATTTCCTGGTGGATGTCTACTTTCATTAACTAGGGTAACATCAGCCATGGCCGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGAATTCATTATTACCAAACACTAAGATATTGGAGAAAGAATTTTTTAGAGAGACAGAGTGAAATACTTGCTCTTGGATTCAATGAAAATTTCATTCGGACATGGGAATATTATTTTGATTATTGTGCTGCTGCTTTCAAGTCATATATATTTAGAGATTACCAGATTGTGTTTGCACGTCCTGGTCAAGCTCTCACATTCAACAATCCATATAAGGGTTTTCCTTCAGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

443

Amino Acids

51.22

Weight (kDa)

6.47

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 148 - 415 6.4e-79 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 194 - 328 5.8e-12 Methyltransferase domain
Methyltransf_31 PF13847 206 - 332 1e-14 Methyltransferase domain
MTS PF05175 206 - 311 5.4e-08 Methyltransferase small domain
Methyltransf_25 PF13649 211 - 307 1.5e-18 Methyltransferase domain
Methyltransf_11 PF08241 212 - 311 4.8e-16 Methyltransferase domain
Methyltransf_12 PF08242 212 - 309 3.1e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccI GTMKAC 1 cut(s) 1021
AciI CCGC 3 cut(s) 53, 799, 1056
AclWI GGATC 1 cut(s) 782
AcoI YGGCCR 2 cut(s) 862, 1053
AcsI RAATTY 5 cut(s) 731, 869, 1098, 1135, 1183
AcuI CTGAAG 2 cut(s) 749, 1305
AfaI GTAC 2 cut(s) 592, 658
AfiI CCNNNNNNNGG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 856
AgsI TTSAA 7 cut(s) 274, 419, 671, 984, 1177, 1234, 1297
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 2 cut(s) 1009, 1276
AluBI AGCT 5 cut(s) 452, 577, 665, 987, 1286
AluI AGCT 5 cut(s) 452, 577, 665, 987, 1286
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 2 cut(s) 403, 1141
AlwI GGATC 1 cut(s) 782
AoxI GGCC 2 cut(s) 862, 1053
ApeKI GCWGC 2 cut(s) 1223, 1226
ApoI RAATTY 5 cut(s) 731, 869, 1098, 1135, 1183
Asp700I GAANNNNTTC 1 cut(s) 835
Asp718I GGTACC 1 cut(s) 656
AsuHPI GGTGA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 656
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 2 cut(s) 1210, 1213
BccI CCATC 2 cut(s) 116, 357
BciT130I CCWGG 2 cut(s) 1011, 1278
BcoDI GTCTC 2 cut(s) 403, 1141
BfaI CTAG 7 cut(s) 72, 233, 609, 851, 908, 1035, 1065
BfmI CTRYAG 2 cut(s) 393, 932
BisI GCNGC 4 cut(s) 54, 1056, 1224, 1227
BlsI GCNGC 4 cut(s) 55, 1057, 1225, 1228
Bme1390I CCNGG 2 cut(s) 1011, 1278
BmgBI CACGTC 1 cut(s) 1274
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 1011, 1278
BmsI GCATC 3 cut(s) 235, 745, 837
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 2 cut(s) 578, 661
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 1050
BsaWI WCCGGW 2 cut(s) 771, 949
Bsc4I CCNNNNNNNGG 1 cut(s) 1310
Bse1I ACTGG 3 cut(s) 103, 212, 706
Bse3DI GCAATG 2 cut(s) 237, 315
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 2 cut(s) 1011, 1278
BseDI CCNNGG 1 cut(s) 1050
BseGI GGATG 6 cut(s) 107, 368, 650, 883, 958, 1022
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 1310
BseMI GCAATG 2 cut(s) 237, 315
BseMII CTCAG 4 cut(s) 212, 414, 473, 708
BseNI ACTGG 3 cut(s) 103, 212, 706
BseXI GCAGC 2 cut(s) 1210, 1213
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 2 cut(s) 864, 1055
BshNI GGYRCC 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 2 cut(s) 772, 950
BslI CCNNNNNNNGG 1 cut(s) 1310
BsmAI GTCTC 2 cut(s) 403, 1141
BsnI GGCC 2 cut(s) 864, 1055
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp143I GATC 3 cut(s) 283, 478, 774
Bsp19I CCATGG 1 cut(s) 1050
BspACI CCGC 3 cut(s) 53, 799, 1056
BspANI GGCC 2 cut(s) 864, 1055
BspCNI CTCAG 4 cut(s) 211, 413, 474, 709
BspLI GGNNCC 1 cut(s) 658
BspMAI CTGCAG 1 cut(s) 936
BspPI GGATC 1 cut(s) 782
BspT107I GGYRCC 1 cut(s) 656
BsrDI GCAATG 2 cut(s) 237, 315
BsrI ACTGG 3 cut(s) 103, 212, 706
BssECI CCNNGG 1 cut(s) 1050
BssMI GATC 3 cut(s) 283, 478, 774
BssT1I CCWWGG 1 cut(s) 1050
Bst2UI CCWGG 2 cut(s) 1011, 1278
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 7 cut(s) 198, 400, 482, 578, 661, 717, 1118
BstDSI CCRYGG 1 cut(s) 1050
BstF5I GGATG 6 cut(s) 107, 368, 650, 883, 958, 1022
BstKTI GATC 3 cut(s) 286, 481, 777
BstMAI GTCTC 2 cut(s) 403, 1141
BstMBI GATC 3 cut(s) 283, 478, 774
BstMWI GCNNNNNNNGC 2 cut(s) 671, 1055
BstNI CCWGG 2 cut(s) 1011, 1278
BstNSI RCATGY 2 cut(s) 425, 860
BstSCI CCNGG 2 cut(s) 1009, 1276
BstSFI CTRYAG 2 cut(s) 393, 932
BstV1I GCAGC 2 cut(s) 1210, 1213
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 2 cut(s) 864, 1055
BtgI CCRYGG 1 cut(s) 1050
BtrI CACGTC 1 cut(s) 1274
BtsCI GGATG 6 cut(s) 107, 368, 650, 883, 958, 1022
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 531, 699
Cac8I GCNNGC 1 cut(s) 58
Csp6I GTAC 2 cut(s) 591, 657
CviAII CATG 7 cut(s) 422, 626, 857, 866, 891, 1051, 1197
CviQI GTAC 2 cut(s) 591, 657
DdeI CTNAG 7 cut(s) 198, 400, 482, 578, 661, 717, 1118
DpnI GATC 3 cut(s) 285, 480, 776
DpnII GATC 3 cut(s) 283, 478, 774
EaeI YGGCCR 2 cut(s) 862, 1053
Eco130I CCWWGG 1 cut(s) 1050
Eco57I CTGAAG 2 cut(s) 749, 1305
EcoRI GAATTC 1 cut(s) 1098
EcoRII CCWGG 2 cut(s) 1009, 1276
EcoT14I CCWWGG 1 cut(s) 1050
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 1050
FaeI CATG 7 cut(s) 425, 629, 860, 869, 894, 1054, 1200
FatI CATG 7 cut(s) 421, 625, 856, 865, 890, 1050, 1196
FauNDI CATATG 1 cut(s) 27
FblI GTMKAC 1 cut(s) 1021
Fnu4HI GCNGC 4 cut(s) 54, 1056, 1224, 1227
FokI GGATG 6 cut(s) 114, 375, 657, 890, 965, 1029
Fsp4HI GCNGC 4 cut(s) 54, 1056, 1224, 1227
FspBI CTAG 7 cut(s) 72, 233, 609, 851, 908, 1035, 1065
GluI GCNGC 4 cut(s) 54, 1056, 1224, 1227
HaeIII GGCC 2 cut(s) 864, 1055
HapII CCGG 2 cut(s) 772, 950
Hin1II CATG 7 cut(s) 425, 629, 860, 869, 894, 1054, 1200
HindIII AAGCTT 1 cut(s) 985
HinfI GANTC 6 cut(s) 329, 831, 899, 922, 1068, 1173
HpaII CCGG 2 cut(s) 772, 950
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 1022
Hpy188I TCNGA 4 cut(s) 483, 546, 718, 1194
Hpy188III TCNNGA 2 cut(s) 635, 1065
Hpy8I GTNNAC 1 cut(s) 1022
HpyAV CCTTC 3 cut(s) 450, 794, 1329
HpyCH4IV ACGT 1 cut(s) 1273
HpyCH4V TGCA 7 cut(s) 242, 248, 536, 572, 693, 934, 1271
HpyF10VI GCNNNNNNNGC 2 cut(s) 671, 1055
HpyF3I CTNAG 7 cut(s) 198, 400, 482, 578, 661, 717, 1118
HpySE526I ACGT 1 cut(s) 1273
Hsp92II CATG 7 cut(s) 425, 629, 860, 869, 894, 1054, 1200
KpnI GGTACC 1 cut(s) 660
Kzo9I GATC 3 cut(s) 283, 478, 774
LmnI GCTCC 1 cut(s) 1078
Lsp1109I GCAGC 2 cut(s) 1210, 1213
LweI GCATC 3 cut(s) 235, 745, 837
MaeI CTAG 7 cut(s) 72, 233, 609, 851, 908, 1035, 1065
MaeII ACGT 1 cut(s) 1273
MaeIII GTNAC 3 cut(s) 67, 791, 1039
MalI GATC 3 cut(s) 285, 480, 776
MboI GATC 3 cut(s) 283, 478, 774
MboII GAAGA 4 cut(s) 131, 284, 563, 1053
MfeI CAATTG 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 1083
MluCI AATT 7 cut(s) 612, 731, 803, 869, 1098, 1135, 1183
MlyI GAGTC 2 cut(s) 908, 1062
Mph1103I ATGCAT 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 835
MseI TTAA 8 cut(s) 107, 113, 183, 428, 729, 992, 1031, 1330
MslI CAYNNNNRTG 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 772, 950
MspR9I CCNGG 2 cut(s) 1011, 1278
MunI CAATTG 1 cut(s) 803
MvaI CCWGG 2 cut(s) 1011, 1278
MwoI GCNNNNNNNGC 2 cut(s) 671, 1055
NcoI CCATGG 1 cut(s) 1050
NdeI CATATG 1 cut(s) 27
NdeII GATC 3 cut(s) 283, 478, 774
NlaIII CATG 7 cut(s) 425, 629, 860, 869, 894, 1054, 1200
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 2 cut(s) 425, 860
PciI ACATGT 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 835
PfeI GAWTC 4 cut(s) 329, 831, 922, 1173
PkrI GCNGC 4 cut(s) 55, 1057, 1225, 1228
PleI GAGTC 2 cut(s) 907, 1062
PpsI GAGTC 2 cut(s) 907, 1062
PscI ACATGT 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 1009, 1276
PspGI CCWGG 2 cut(s) 1009, 1276
PspN4I GGNNCC 1 cut(s) 658
PstI CTGCAG 1 cut(s) 936
RsaI GTAC 2 cut(s) 592, 658
RsaNI GTAC 2 cut(s) 591, 657
RseI CAYNNNNRTG 1 cut(s) 822
SaqAI TTAA 8 cut(s) 107, 113, 183, 428, 729, 992, 1031, 1330
SatI GCNGC 4 cut(s) 54, 1056, 1224, 1227
Sau3AI GATC 3 cut(s) 283, 478, 774
SchI GAGTC 2 cut(s) 908, 1062
ScrFI CCNGG 2 cut(s) 1011, 1278
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 3 cut(s) 235, 745, 837
SfcI CTRYAG 2 cut(s) 393, 932
SmiMI CAYNNNNRTG 1 cut(s) 822
Sse9I AATT 7 cut(s) 612, 731, 803, 869, 1098, 1135, 1183
SsiI CCGC 3 cut(s) 53, 799, 1056
SspI AATATT 3 cut(s) 410, 540, 1205
SspMI CTAG 7 cut(s) 72, 233, 609, 851, 908, 1035, 1065
StyD4I CCNGG 2 cut(s) 1009, 1276
StyI CCWWGG 1 cut(s) 1050
TaiI ACGT 1 cut(s) 1276
TaqI TCGA 3 cut(s) 286, 347, 454
TasI AATT 7 cut(s) 612, 731, 803, 869, 1098, 1135, 1183
TatI WGTACW 1 cut(s) 590
TauI GCSGC 2 cut(s) 56, 1058
TfiI GAWTC 4 cut(s) 329, 831, 922, 1173
Tru1I TTAA 8 cut(s) 107, 113, 183, 428, 729, 992, 1031, 1330
Tru9I TTAA 8 cut(s) 107, 113, 183, 428, 729, 992, 1031, 1330
TscAI CASTG 2 cut(s) 538, 706
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 2 cut(s) 1223, 1226
Tsp45I GTSAC 1 cut(s) 791
TspGWI ACGGA 2 cut(s) 59, 166
TspRI CASTG 2 cut(s) 538, 706
XapI RAATTY 5 cut(s) 731, 869, 1098, 1135, 1183
XbaI TCTAGA 1 cut(s) 1064
XceI RCATGY 2 cut(s) 425, 860
XmiI GTMKAC 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 835
XspI CTAG 7 cut(s) 72, 233, 609, 851, 908, 1035, 1065
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.