FvH4_7g18140

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000555
Physical Location & Seq
Reverse (-)
15228279 .. 15232054
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18140.t1

Sequence Viewer

Length: 1302 bp
ATGGCTTCAGCAATATTGGAAAGGGTCGCTCGCCTATATGTTACCAAATTTCTACGACAGTATGTCTCTACAGGATGTTTGGTCTTATCAGAAGATGGAGGAACGGTAGAAACCTTTGAGGGAACCAAAAAAGGCTGTTCTTTGAAATGTGTTCTTCAAGTTCATGATCCCGCTCAATTTTACTGGAAGGTAATGACAAGGGCAGATCTAGGCCTTGCAGATGCATATATCAATGGTGATTTTTCTTTAGCTGATAAAGACAAGGGTCTGGAGAATCTCTTTACGATTCTTAATGCCAACAGAGATTCAACTTCTCCACTCTCAAAATTGAACAAGATAAGAAGAAGATTACTGGAAGCTTGTTTATCCCCAGCAAGATATCTCTTTCACCACGGTTCAGGGCAAAACACACTTACAGTGGCTCGAAGGAACGTCTCTCGCCATTATGACTTGAGTAATGCACTATTTTCGCTCTTCTTGGACGAAACAATGTCGTATTCCACTGCTGTATTCAAGAAAGATGATGAAGATTTGAAGACTGGACAGCTAAGGAAAATCAAGACTCTCATTGACAAGGCTAGAATTACTAAGAACCATGAAGTCCTAGAGGTAGGCTGTGGTTGGGGAGGCTTAGCTATCGAAGTCGTCAAGCAAACCGGTTGTAGATATACTGGCATAACTTTGTCGAAGGAACAACTGCAATTTGCCGAACAGAGAGTGAAAGATGCTGGACTTGAGGAAAACATCAAATTTCTGCTGTGTGACTACCGCACATTGCCTAAGAACTACAAATATGACAGAATTGTTAGTTGCGAGATGGTAGAACATGTCGGCCATGAATATATGGAGGAATTCTTTGCTTGCTGTGACTCTGCTCTAGCAGAAAATGGGCTTCTCGTTCTACAGTTCACATCATTACCGGATGAGCTTTACGATGAGTACAGGCGATGTCCAAGCTTCATAAGGGAGTATATTTTTCCAGGTGGATGTTTACCTTCGTTAAGTAGGTTAACAGCTGCCATGGCCGCTTCTTCCAGGCTTAGTGTGGAGCACTTGGAAAACATTGGAAGTCATTATTACCGAACACTAAAATATTGGAGGAAAAAATTCCTGGAGAAACAAAGTGAAATACTAGCTCTTGGATTCGATGACAAGTTTATTCGGACATGGGAATATTATTTTGATTATTGTGCGGCAGGATTCAACGCTTGTACACTTGGGAATTACCAGATTGTGTTTTCACGTCCCGGTAATGTTGCTCTGCTCAACGATCCATACAAAGGCTTCCCTTCGGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

434

Amino Acids

49.34

Weight (kDa)

7.84

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 140 - 406 5.9e-78 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 186 - 309 1.4e-09 Methyltransferase domain
MTS PF05175 191 - 301 2.3e-06 Methyltransferase small domain
Methyltransf_31 PF13847 196 - 305 6.5e-12 Methyltransferase domain
Methyltransf_25 PF13649 201 - 297 8e-17 Methyltransferase domain
Methyltransf_11 PF08241 202 - 301 1.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 202 - 299 7.5e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 173
AciI CCGC 4 cut(s) 171, 769, 1026, 1193
AclWI GGATC 2 cut(s) 161, 1265
AcoI YGGCCR 2 cut(s) 832, 1023
AcsI RAATTY 4 cut(s) 47, 749, 851, 1106
AfaI GTAC 2 cut(s) 941, 1213
AfiI CCNNNNNNNGG 1 cut(s) 1280
AflIII ACRYGT 1 cut(s) 826
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 7 cut(s) 145, 158, 309, 331, 514, 535, 1204
AhdI GACNNNNNGTC 1 cut(s) 62
AjiI CACGTC 1 cut(s) 1244
AjnI CCWGG 3 cut(s) 979, 1034, 1110
AluBI AGCT 8 cut(s) 251, 359, 547, 635, 928, 957, 1016, 1136
AluI AGCT 8 cut(s) 251, 359, 547, 635, 928, 957, 1016, 1136
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 2 cut(s) 70, 439
AlwI GGATC 2 cut(s) 161, 1265
AoxI GGCC 3 cut(s) 211, 832, 1023
ApeKI GCWGC 1 cut(s) 1016
ApoI RAATTY 4 cut(s) 47, 749, 851, 1106
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 1106
AsuC2I CCSGG 1 cut(s) 1248
AsuHPI GGTGA 2 cut(s) 248, 380
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 1 cut(s) 1003
BccI CCATC 2 cut(s) 89, 811
BcgI CGANNNNNNTGC 2 cut(s) 450, 484
BciT130I CCWGG 3 cut(s) 981, 1036, 1112
BcnI CCSGG 1 cut(s) 1248
BcoDI GTCTC 2 cut(s) 70, 439
BfaI CTAG 5 cut(s) 209, 579, 605, 878, 1133
BfmI CTRYAG 2 cut(s) 69, 902
BglII AGATCT 1 cut(s) 205
BisI GCNGC 3 cut(s) 1017, 1026, 1194
BlpI GCTNAGC 1 cut(s) 631
BlsI GCNGC 3 cut(s) 1018, 1027, 1195
Bme1390I CCNGG 4 cut(s) 981, 1036, 1112, 1248
BmeRI GACNNNNNGTC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 1244
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 4 cut(s) 981, 1036, 1112, 1248
BmsI GCATC 2 cut(s) 211, 715
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 2 cut(s) 290, 1133
Bpu10I CCTNAGC 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 631
BpuEI CTTGAG 2 cut(s) 472, 755
BpuMI CCSGG 1 cut(s) 1248
BsaJI CCNNGG 2 cut(s) 391, 1020
BsaWI WCCGGW 2 cut(s) 656, 919
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 1280
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 4 cut(s) 188, 357, 544, 676
Bse3DI GCAATG 1 cut(s) 773
BseBI CCWGG 3 cut(s) 981, 1036, 1112
BseDI CCNNGG 2 cut(s) 391, 1020
BseGI GGATG 3 cut(s) 80, 928, 992
BseLI CCNNNNNNNGG 1 cut(s) 1280
BseMI GCAATG 1 cut(s) 773
BseNI ACTGG 4 cut(s) 188, 357, 544, 676
BseXI GCAGC 1 cut(s) 1003
BseYI CCCAGC 1 cut(s) 370
BshFI GGCC 3 cut(s) 213, 834, 1025
BshTI ACCGGT 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 1053
BsiSI CCGG 3 cut(s) 657, 920, 1248
BslFI GGGAC 1 cut(s) 1230
BslI CCNNNNNNNGG 1 cut(s) 1280
BsmAI GTCTC 2 cut(s) 70, 439
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 1230
BsnI GGCC 3 cut(s) 213, 834, 1025
Bsp1286I GDGCHC 1 cut(s) 1053
Bsp1407I TGTACA 1 cut(s) 1211
Bsp143I GATC 3 cut(s) 166, 205, 1270
Bsp1720I GCTNAGC 1 cut(s) 631
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 4 cut(s) 171, 769, 1026, 1193
BspANI GGCC 3 cut(s) 213, 834, 1025
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 124
BspPI GGATC 2 cut(s) 161, 1265
BspQI GCTCTTC 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 773
BsrFI RCCGGY 1 cut(s) 656
BsrGI TGTACA 1 cut(s) 1211
BsrI ACTGG 4 cut(s) 188, 357, 544, 676
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 2 cut(s) 391, 1020
BssMI GATC 3 cut(s) 166, 205, 1270
BssT1I CCWWGG 1 cut(s) 1020
Bst2UI CCWGG 3 cut(s) 981, 1036, 1112
Bst4CI ACNGT 5 cut(s) 60, 106, 395, 418, 906
Bst6I CTCTTC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 1211
BstC8I GCNNGC 2 cut(s) 31, 862
BstDEI CTNAG 5 cut(s) 548, 588, 631, 780, 1040
BstDSI CCRYGG 2 cut(s) 391, 1020
BstF5I GGATG 3 cut(s) 80, 928, 992
BstKTI GATC 3 cut(s) 169, 208, 1273
BstMAI GTCTC 2 cut(s) 70, 439
BstMBI GATC 3 cut(s) 166, 205, 1270
BstMWI GCNNNNNNNGC 2 cut(s) 1022, 1025
BstNI CCWGG 3 cut(s) 981, 1036, 1112
BstNSI RCATGY 1 cut(s) 830
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 4 cut(s) 979, 1034, 1110, 1246
BstSFI CTRYAG 2 cut(s) 69, 902
BstV1I GCAGC 1 cut(s) 1003
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 3 cut(s) 213, 834, 1025
BtgI CCRYGG 2 cut(s) 391, 1020
BtgZI GCGATG 1 cut(s) 961
BtrI CACGTC 1 cut(s) 1244
BtsCI GGATG 3 cut(s) 80, 928, 992
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 2 cut(s) 423, 501
Cac8I GCNNGC 2 cut(s) 31, 862
CciI TCATGA 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 656
Csp6I GTAC 2 cut(s) 940, 1212
CspAI ACCGGT 1 cut(s) 656
CviAII CATG 6 cut(s) 164, 596, 827, 836, 1021, 1167
CviQI GTAC 2 cut(s) 940, 1212
DdeI CTNAG 5 cut(s) 548, 588, 631, 780, 1040
DpnI GATC 3 cut(s) 168, 207, 1272
DpnII GATC 3 cut(s) 166, 205, 1270
DriI GACNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 832, 1023
Eam1104I CTCTTC 1 cut(s) 479
Eam1105I GACNNNNNGTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 479
Eco130I CCWWGG 1 cut(s) 1020
Eco147I AGGCCT 1 cut(s) 213
Eco32I GATATC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 851
EcoRII CCWGG 3 cut(s) 979, 1034, 1110
EcoRV GATATC 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 1020
EcoT22I ATGCAT 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 1020
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 6 cut(s) 167, 599, 830, 839, 1024, 1170
FaqI GGGAC 1 cut(s) 1230
FatI CATG 6 cut(s) 163, 595, 826, 835, 1020, 1166
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 3 cut(s) 1017, 1026, 1194
FokI GGATG 3 cut(s) 87, 935, 999
Fsp4HI GCNGC 3 cut(s) 1017, 1026, 1194
FspBI CTAG 5 cut(s) 209, 579, 605, 878, 1133
GluI GCNGC 3 cut(s) 1017, 1026, 1194
GsaI CCCAGC 1 cut(s) 374
GsuI CTGGAG 2 cut(s) 290, 1133
HaeIII GGCC 3 cut(s) 213, 834, 1025
HapII CCGG 3 cut(s) 657, 920, 1248
Hin1II CATG 6 cut(s) 167, 599, 830, 839, 1024, 1170
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HindIII AAGCTT 2 cut(s) 357, 955
HinfI GANTC 7 cut(s) 274, 286, 305, 562, 869, 1143, 1200
HpaI GTTAAC 1 cut(s) 1011
HpaII CCGG 3 cut(s) 657, 920, 1248
HphI GGTGA 2 cut(s) 248, 380
Hpy166II GTNNAC 4 cut(s) 909, 992, 1011, 1214
Hpy188I TCNGA 2 cut(s) 91, 1164
Hpy188III TCNNGA 4 cut(s) 164, 269, 514, 559
Hpy8I GTNNAC 4 cut(s) 909, 992, 1011, 1214
HpyAV CCTTC 5 cut(s) 181, 420, 682, 1005, 1299
HpyCH4III ACNGT 5 cut(s) 60, 106, 395, 418, 906
HpyCH4IV ACGT 2 cut(s) 432, 1243
HpyCH4V TGCA 4 cut(s) 218, 224, 461, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 1022, 1025
HpyF3I CTNAG 5 cut(s) 548, 588, 631, 780, 1040
HpySE526I ACGT 2 cut(s) 432, 1243
Hsp92II CATG 6 cut(s) 167, 599, 830, 839, 1024, 1170
KspAI GTTAAC 1 cut(s) 1011
Kzo9I GATC 3 cut(s) 166, 205, 1270
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 1 cut(s) 1048
Lsp1109I GCAGC 1 cut(s) 1003
LweI GCATC 2 cut(s) 211, 715
MaeI CTAG 5 cut(s) 209, 579, 605, 878, 1133
MaeII ACGT 2 cut(s) 432, 1243
MaeIII GTNAC 3 cut(s) 40, 761, 866
MalI GATC 3 cut(s) 168, 207, 1272
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 166, 205, 1270
MboII GAAGA 8 cut(s) 104, 146, 354, 357, 466, 539, 547, 1023
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 1053
MlyI GAGTC 2 cut(s) 556, 863
MnlI CCTC 7 cut(s) 92, 112, 601, 620, 730, 841, 1092
Mph1103I ATGCAT 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 1106
MseI TTAA 3 cut(s) 291, 1001, 1010
MspA1I CMGCKG 1 cut(s) 1016
MspI CCGG 3 cut(s) 657, 920, 1248
MspR9I CCNGG 4 cut(s) 981, 1036, 1112, 1248
MvaI CCWGG 3 cut(s) 981, 1036, 1112
MwoI GCNNNNNNNGC 2 cut(s) 1022, 1025
NciI CCSGG 1 cut(s) 1248
NcoI CCATGG 1 cut(s) 1020
NdeII GATC 3 cut(s) 166, 205, 1270
NlaIII CATG 6 cut(s) 167, 599, 830, 839, 1024, 1170
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 2 cut(s) 761, 866
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 830
PagI TCATGA 1 cut(s) 163
PceI AGGCCT 1 cut(s) 213
PciI ACATGT 1 cut(s) 826
PciSI GCTCTTC 1 cut(s) 479
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 5 cut(s) 274, 286, 305, 1143, 1200
PfoI TCCNGGA 1 cut(s) 1110
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 3 cut(s) 1018, 1027, 1195
PleI GAGTC 2 cut(s) 556, 863
PpsI GAGTC 2 cut(s) 556, 863
PscI ACATGT 1 cut(s) 826
PshAI GACNNNNGTC 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 979, 1034, 1110
PspFI CCCAGC 1 cut(s) 370
PspGI CCWGG 3 cut(s) 979, 1034, 1110
PspN4I GGNNCC 1 cut(s) 124
PsuI RGATCY 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 2 cut(s) 941, 1213
RsaNI GTAC 2 cut(s) 940, 1212
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 3 cut(s) 291, 1001, 1010
SatI GCNGC 3 cut(s) 1017, 1026, 1194
Sau3AI GATC 3 cut(s) 166, 205, 1270
SchI GAGTC 2 cut(s) 556, 863
ScrFI CCNGG 4 cut(s) 981, 1036, 1112, 1248
SduI GDGCHC 1 cut(s) 1053
SfaNI GCATC 2 cut(s) 211, 715
SfcI CTRYAG 2 cut(s) 69, 902
SmlI CTYRAG 2 cut(s) 451, 734
SmoI CTYRAG 2 cut(s) 451, 734
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 4 cut(s) 171, 769, 1026, 1193
SspI AATATT 3 cut(s) 15, 1094, 1175
SspMI CTAG 5 cut(s) 209, 579, 605, 878, 1133
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 4 cut(s) 979, 1034, 1110, 1246
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 5 cut(s) 60, 106, 395, 418, 906
TaiI ACGT 2 cut(s) 435, 1246
TaqI TCGA 4 cut(s) 424, 639, 686, 1146
TatI WGTACW 2 cut(s) 939, 1211
TauI GCSGC 2 cut(s) 1028, 1196
TfiI GAWTC 5 cut(s) 274, 286, 305, 1143, 1200
Tru1I TTAA 3 cut(s) 291, 1001, 1010
Tru9I TTAA 3 cut(s) 291, 1001, 1010
TscAI CASTG 2 cut(s) 423, 508
TseFI GTSAC 2 cut(s) 761, 866
TseI GCWGC 1 cut(s) 1016
Tsp45I GTSAC 2 cut(s) 761, 866
TspDTI ATGAA 5 cut(s) 152, 540, 612, 852, 949
TspRI CASTG 2 cut(s) 423, 508
XapI RAATTY 4 cut(s) 47, 749, 851, 1106
XceI RCATGY 1 cut(s) 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 5 cut(s) 209, 579, 605, 878, 1133
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.