RchiOBHm_Chr6g0273911

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000555
Physical Location & Seq
Reverse (-)
34939334 .. 34942700
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24573

Sequence Viewer

Length: 1284 bp
ATGGCTCTTTCGGACAACCCAAAACGTATGGCTCTTTCATTGACCGAAACAGCGGCACGCCTATTTGTTACTAGATTTCTTAGAAATTATATATCTTCTGGATGTTTAATCTTATTAGAAGAGGGAGGCACAGTGTTCACCTTTGAGGGAAGCAAAAAGGATTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCACTTCTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATTAGTGGAGACTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGATTTTGATAAAACTATGCCTTGTTGTTTCTTCTTCCAGCTTCTCATTGCCAACGGAGATTTTAATGCCTCAAAATCAAACAAGAAAAGGTGTAATGCCTCGATTATCTCAAACAAATTTTGATATAGCTGATAATAATCATCCCGGTTTCATTATGTTGTTGGTTTTATGTAGGAGATGGTGGCAGCCTGTGTTTTTCACAGCTACTATAGCCACAGCCAAATATGTCTTCCAGCATGCTTTAAGGAAAAATAGTCTTACACAAGCTCGAAGGAACATATCTCGTCATTATGATTTGAGTAATGATCTATTTTCGCTGTTCTTGGGTGAAACAATGATGTATTCCACTGCAATATTTAAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTCTACTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTAGAAATTGGTTGTGGTTGGGGAGCCTTAGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAAGAGCAACTGAAATTTGCTGAAAAAAAAGTGAAAGATGCTGGACTTCAGGACCGGATCAAGTTTCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATAATTTGCGAGGTGCTAGAACATGTCGGCCATGAATTTGTGGATGAGTTTTTTTCTTGCTGTGACTCTTTACTAGCAGAAAATGGGATTCTTGTTCTGCAGTTCATATCTATACTGGATGAACTCTATGATGAGTATTGGCGAAGTCCAGGCTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACTTTCATTAAGTAGGTTAACATCAGCCATGGCTGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGACATCATCATTATTACCAAACACTAAGATGTTGGAGAAATAATTTTTTAGAGAGACAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

427

Amino Acids

49.04

Weight (kDa)

6.51

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 189 - 425 3.3e-66 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 235 - 359 6.6e-11 Methyltransferase domain
Methyltransf_31 PF13847 247 - 362 2e-13 Methyltransferase domain
MTS PF05175 247 - 352 1.1e-06 Methyltransferase small domain
Methyltransf_25 PF13649 252 - 348 8.8e-17 Methyltransferase domain
Methyltransf_11 PF08241 253 - 352 1.8e-14 Methyltransferase domain
Methyltransf_12 PF08242 253 - 350 5.3e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1144
AciI CCGC 2 cut(s) 53, 922
AclWI GGATC 1 cut(s) 905
AcoI YGGCCR 1 cut(s) 985
AcsI RAATTY 3 cut(s) 424, 854, 992
AcuI CTGAAG 2 cut(s) 861, 872
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 1 cut(s) 794
AjnI CCWGG 2 cut(s) 1105, 1132
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AluBI AGCT 6 cut(s) 348, 437, 512, 575, 700, 788
AluI AGCT 6 cut(s) 348, 437, 512, 575, 700, 788
Alw21I GWGCWC 1 cut(s) 1206
Alw26I GTCTC 2 cut(s) 255, 1267
AlwI GGATC 1 cut(s) 905
AoxI GGCC 1 cut(s) 985
ApeKI GCWGC 2 cut(s) 493, 1178
ApoI RAATTY 3 cut(s) 424, 854, 992
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AspS9I GGNCC 1 cut(s) 892
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 130, 647
AvaII GGWCC 1 cut(s) 892
BbsI GAAGAC 2 cut(s) 284, 529
Bbv12I GWGCWC 1 cut(s) 1206
BbvI GCAGC 2 cut(s) 505, 1165
BccI CCATC 1 cut(s) 480
BciT130I CCWGG 2 cut(s) 1107, 1134
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 2 cut(s) 255, 1267
BfaI CTAG 7 cut(s) 72, 233, 732, 758, 974, 1031, 1188
BfmI CTRYAG 2 cut(s) 516, 1055
BisI GCNGC 3 cut(s) 54, 494, 1179
BlsI GCNGC 3 cut(s) 55, 495, 1180
Bme1390I CCNGG 3 cut(s) 453, 1107, 1134
Bme18I GGWCC 1 cut(s) 892
BmgT120I GGNCC 1 cut(s) 892
BmiI GGNNCC 1 cut(s) 781
BmrFI CCNGG 3 cut(s) 453, 1107, 1134
BmsI GCATC 3 cut(s) 235, 837, 868
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 2 cut(s) 284, 529
Bpu10I CCTNAGC 2 cut(s) 701, 784
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 286, 1173
BsaWI WCCGGW 1 cut(s) 894
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bse1I ACTGG 3 cut(s) 212, 829, 1077
Bse3DI GCAATG 2 cut(s) 237, 353
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 2 cut(s) 1107, 1134
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 2 cut(s) 286, 1173
BseGI GGATG 5 cut(s) 107, 448, 1006, 1081, 1145
BseJI GATNNNNATC 1 cut(s) 444
BseMI GCAATG 2 cut(s) 237, 353
BseNI ACTGG 3 cut(s) 212, 829, 1077
BseXI GCAGC 2 cut(s) 505, 1165
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 1 cut(s) 987
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 1 cut(s) 1206
BsiSI CCGG 2 cut(s) 453, 895
BsmAI GTCTC 2 cut(s) 255, 1267
BsnI GGCC 1 cut(s) 987
Bsp1286I GDGCHC 1 cut(s) 1206
Bsp143I GATC 3 cut(s) 229, 613, 897
Bsp19I CCATGG 1 cut(s) 1173
BspACI CCGC 2 cut(s) 53, 922
BspANI GGCC 1 cut(s) 987
BspDI ATCGAT 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 781
BspMAI CTGCAG 1 cut(s) 1059
BspPI GGATC 1 cut(s) 905
BspQI GCTCTTC 1 cut(s) 837
BsrDI GCAATG 2 cut(s) 237, 353
BsrI ACTGG 3 cut(s) 212, 829, 1077
BssECI CCNNGG 2 cut(s) 286, 1173
BssMI GATC 3 cut(s) 229, 613, 897
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 2 cut(s) 1107, 1134
Bst4CI ACNGT 2 cut(s) 133, 194
Bst6I CTCTTC 2 cut(s) 114, 837
BstC8I GCNNGC 2 cut(s) 58, 546
BstDEI CTNAG 4 cut(s) 80, 701, 784, 1244
BstDSI CCRYGG 1 cut(s) 1173
BstF5I GGATG 5 cut(s) 107, 448, 1006, 1081, 1145
BstKTI GATC 3 cut(s) 232, 616, 900
BstMAI GTCTC 2 cut(s) 255, 1267
BstMBI GATC 3 cut(s) 229, 613, 897
BstMWI GCNNNNNNNGC 3 cut(s) 518, 794, 1178
BstNI CCWGG 2 cut(s) 1107, 1134
BstNSI RCATGY 2 cut(s) 548, 983
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 451, 1105, 1132
BstSFI CTRYAG 2 cut(s) 516, 1055
BstV1I GCAGC 2 cut(s) 505, 1165
BstV2I GAAGAC 2 cut(s) 284, 529
Bsu15I ATCGAT 1 cut(s) 738
BsuRI GGCC 1 cut(s) 987
BsuTUI ATCGAT 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 1173
BtsCI GGATG 5 cut(s) 107, 448, 1006, 1081, 1145
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 138, 654, 822
Cac8I GCNNGC 2 cut(s) 58, 546
Cfr13I GGNCC 1 cut(s) 892
ClaI ATCGAT 1 cut(s) 738
CviAII CATG 5 cut(s) 545, 749, 980, 989, 1174
DdeI CTNAG 4 cut(s) 80, 701, 784, 1244
DpnI GATC 3 cut(s) 231, 615, 899
DpnII GATC 3 cut(s) 229, 613, 897
EaeI YGGCCR 1 cut(s) 985
Eam1104I CTCTTC 2 cut(s) 114, 837
EarI CTCTTC 2 cut(s) 114, 837
Eco130I CCWWGG 1 cut(s) 1173
Eco47I GGWCC 1 cut(s) 892
Eco57I CTGAAG 2 cut(s) 861, 872
EcoRII CCWGG 2 cut(s) 1105, 1132
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 5 cut(s) 548, 752, 983, 992, 1177
FatI CATG 5 cut(s) 544, 748, 979, 988, 1173
FblI GTMKAC 1 cut(s) 1144
Fnu4HI GCNGC 3 cut(s) 54, 494, 1179
FokI GGATG 5 cut(s) 114, 435, 1013, 1088, 1152
Fsp4HI GCNGC 3 cut(s) 54, 494, 1179
FspBI CTAG 7 cut(s) 72, 233, 732, 758, 974, 1031, 1188
GluI GCNGC 3 cut(s) 54, 494, 1179
HaeIII GGCC 1 cut(s) 987
HapII CCGG 2 cut(s) 453, 895
Hin1II CATG 5 cut(s) 548, 752, 983, 992, 1177
HincII GTYRAC 1 cut(s) 1164
HindII GTYRAC 1 cut(s) 1164
HinfI GANTC 5 cut(s) 735, 954, 1022, 1045, 1191
HpaI GTTAAC 1 cut(s) 1164
HpaII CCGG 2 cut(s) 453, 895
HphI GGTGA 2 cut(s) 130, 647
Hpy166II GTNNAC 4 cut(s) 138, 190, 1145, 1164
Hpy188I TCNGA 3 cut(s) 13, 841, 937
Hpy188III TCNNGA 4 cut(s) 99, 758, 890, 1188
Hpy8I GTNNAC 4 cut(s) 138, 190, 1145, 1164
HpyAV CCTTC 2 cut(s) 573, 942
HpyCH4III ACNGT 2 cut(s) 133, 194
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 6 cut(s) 242, 248, 659, 695, 816, 1057
HpyF10VI GCNNNNNNNGC 3 cut(s) 518, 794, 1178
HpyF3I CTNAG 4 cut(s) 80, 701, 784, 1244
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 5 cut(s) 548, 752, 983, 992, 1177
KspAI GTTAAC 1 cut(s) 1164
Kzo9I GATC 3 cut(s) 229, 613, 897
LguI GCTCTTC 1 cut(s) 837
LmnI GCTCC 2 cut(s) 779, 1201
Lsp1109I GCAGC 2 cut(s) 505, 1165
LweI GCATC 3 cut(s) 235, 837, 868
MaeI CTAG 7 cut(s) 72, 233, 732, 758, 974, 1031, 1188
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 3 cut(s) 67, 914, 1019
MalI GATC 3 cut(s) 231, 615, 899
MboI GATC 3 cut(s) 229, 613, 897
MfeI CAATTG 1 cut(s) 926
MhlI GDGCHC 1 cut(s) 1206
MluCI AATT 8 cut(s) 85, 424, 762, 854, 926, 960, 992, 1261
MlyI GAGTC 2 cut(s) 1016, 1185
MmeI TCCRAC 1 cut(s) 1232
Mph1103I ATGCAT 1 cut(s) 250
MseI TTAA 6 cut(s) 107, 371, 551, 666, 1154, 1163
MslI CAYNNNNRTG 2 cut(s) 945, 1246
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 453, 895
MspR9I CCNGG 3 cut(s) 453, 1107, 1134
MunI CAATTG 1 cut(s) 926
MvaI CCWGG 2 cut(s) 1107, 1134
MwoI GCNNNNNNNGC 3 cut(s) 518, 794, 1178
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 1173
NdeII GATC 3 cut(s) 229, 613, 897
NlaIII CATG 5 cut(s) 548, 752, 983, 992, 1177
NlaIV GGNNCC 1 cut(s) 781
NmuCI GTSAC 2 cut(s) 914, 1019
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 2 cut(s) 548, 983
PaeI GCATGC 1 cut(s) 548
PciI ACATGT 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 837
PfeI GAWTC 3 cut(s) 735, 954, 1045
PkrI GCNGC 3 cut(s) 55, 495, 1180
PleI GAGTC 2 cut(s) 1016, 1185
PpsI GAGTC 2 cut(s) 1016, 1185
PscI ACATGT 1 cut(s) 979
PshAI GACNNNNGTC 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 1105, 1132
PspGI CCWGG 2 cut(s) 1105, 1132
PspN4I GGNNCC 1 cut(s) 781
PspPI GGNCC 1 cut(s) 892
PstI CTGCAG 1 cut(s) 1059
RseI CAYNNNNRTG 2 cut(s) 945, 1246
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 6 cut(s) 107, 371, 551, 666, 1154, 1163
SatI GCNGC 3 cut(s) 54, 494, 1179
Sau3AI GATC 3 cut(s) 229, 613, 897
Sau96I GGNCC 1 cut(s) 892
SchI GAGTC 2 cut(s) 1016, 1185
ScrFI CCNGG 3 cut(s) 453, 1107, 1134
SduI GDGCHC 1 cut(s) 1206
SfaNI GCATC 3 cut(s) 235, 837, 868
SfcI CTRYAG 2 cut(s) 516, 1055
SinI GGWCC 1 cut(s) 892
SmiMI CAYNNNNRTG 2 cut(s) 945, 1246
SphI GCATGC 1 cut(s) 548
Sse9I AATT 8 cut(s) 85, 424, 762, 854, 926, 960, 992, 1261
SsiI CCGC 2 cut(s) 53, 922
SspI AATATT 1 cut(s) 663
SspMI CTAG 7 cut(s) 72, 233, 732, 758, 974, 1031, 1188
StyD4I CCNGG 3 cut(s) 451, 1105, 1132
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 2 cut(s) 133, 194
TaiI ACGT 1 cut(s) 28
TaqI TCGA 3 cut(s) 409, 577, 738
TaqII GACCGA 2 cut(s) 59, 300
TasI AATT 8 cut(s) 85, 424, 762, 854, 926, 960, 992, 1261
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 3 cut(s) 735, 954, 1045
Tru1I TTAA 6 cut(s) 107, 371, 551, 666, 1154, 1163
Tru9I TTAA 6 cut(s) 107, 371, 551, 666, 1154, 1163
TscAI CASTG 3 cut(s) 138, 661, 829
TseFI GTSAC 2 cut(s) 914, 1019
TseI GCWGC 2 cut(s) 493, 1178
Tsp45I GTSAC 2 cut(s) 914, 1019
TspDTI ATGAA 7 cut(s) 27, 448, 1005, 1051, 1092, 1102, 1140
TspGWI ACGGA 2 cut(s) 166, 377
TspRI CASTG 3 cut(s) 138, 661, 829
VpaK11BI GGWCC 1 cut(s) 892
XapI RAATTY 3 cut(s) 424, 854, 992
XbaI TCTAGA 2 cut(s) 757, 1187
XceI RCATGY 2 cut(s) 548, 983
XmiI GTMKAC 1 cut(s) 1144
XspI CTAG 7 cut(s) 72, 233, 732, 758, 974, 1031, 1188
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.