Rh6AG193200

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000555
Physical Location & Seq
Reverse (-)
34595113 .. 34597177
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG193200.1

Sequence Viewer

Length: 660 bp
ATGGCTCTTTCATTGACCGAAACAGTGGCACGCCTATTTGTTACTAGATTTCTTAGAAATTATATATCTTCTGGATGTTTAATCTTATTAGAAGAGGGAGGCACAGTGTTCACCTTTGAGGGAAGCAAAAAGGATTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCACTTCTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATTAGTGGAGACTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGGCTAGAATCGATGAGAACCATGAGGTTCTAGAAATTGGTTGTGGTTGGGGAGCCTTAGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAAGAGCAACTGAAATTTGCTGAAAAAAAAGTGAAAGATGCTGGACTTCAGGACCGGATCAAGTTTCTTCTGCGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATAATTTGCGAGGTGCTAGAACATGTCGGCCATGAATTTGTGGATGAGTTTTTTTCTTGCTGTGACTCTTTACTAGCAGAAAATGGGATTCTTGTTCTGCAGGTAATGTTTGACAATTTTGACTCCAAGTTGCTAAACTATTCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

219

Amino Acids

24.9

Weight (kDa)

5.09

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTS PF05175 78 - 203 3.7e-09 Methyltransferase small domain
Methyltransf_23 PF13489 89 - 208 7.3e-12 Methyltransferase domain
Methyltransf_18 PF12847 94 - 174 1e-05 Methyltransferase domain
CMAS PF02353 95 - 209 4.4e-28 Mycolic acid cyclopropane synthetase
PrmA PF06325 95 - 190 5.2e-06 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 98 - 204 1.5e-12 Methyltransferase domain
Methyltransf_25 PF13649 103 - 199 4.4e-17 Methyltransferase domain
Methyltransf_12 PF08242 104 - 201 8.6e-15 Methyltransferase domain
Methyltransf_11 PF08241 104 - 203 1.5e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 601
AciI CCGC 1 cut(s) 475
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 407, 545
AcuI CTGAAG 2 cut(s) 414, 425
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 1 cut(s) 347
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 458
AoxI GGCC 1 cut(s) 538
ApoI RAATTY 2 cut(s) 407, 545
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspS9I GGNCC 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 6 cut(s) 45, 206, 285, 311, 527, 584
BfmI CTRYAG 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 601
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 334
BmsI GCATC 3 cut(s) 208, 390, 421
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 337
Bsa29I ATCGAT 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse1I ACTGG 2 cut(s) 185, 382
Bse3DI GCAATG 1 cut(s) 210
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 80, 559
BseMI GCAATG 1 cut(s) 210
BseNI ACTGG 2 cut(s) 185, 382
BshFI GGCC 1 cut(s) 540
BshVI ATCGAT 1 cut(s) 291
BsiSI CCGG 1 cut(s) 448
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 1 cut(s) 540
Bsp143I GATC 2 cut(s) 202, 450
BspACI CCGC 1 cut(s) 475
BspANI GGCC 1 cut(s) 540
BspDI ATCGAT 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 612
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 2 cut(s) 185, 382
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 2 cut(s) 202, 450
Bst4CI ACNGT 3 cut(s) 25, 106, 167
Bst6I CTCTTC 2 cut(s) 87, 390
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 2 cut(s) 53, 337
BstF5I GGATG 2 cut(s) 80, 559
BstKTI GATC 2 cut(s) 205, 453
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 2 cut(s) 202, 450
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 536
BstPAI GACNNNNGTC 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 608
BstV2I GAAGAC 1 cut(s) 257
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 1 cut(s) 540
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 2 cut(s) 80, 559
BtsIMutI CAGTG 3 cut(s) 30, 111, 375
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 291
CviAII CATG 3 cut(s) 302, 533, 542
CviJI RGCY 6 cut(s) 5, 200, 284, 335, 341, 540
CviKI_1 RGCY 6 cut(s) 5, 200, 284, 335, 341, 540
DdeI CTNAG 2 cut(s) 53, 337
DpnI GATC 2 cut(s) 204, 452
DpnII GATC 2 cut(s) 202, 450
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 2 cut(s) 87, 390
EarI CTCTTC 2 cut(s) 87, 390
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 414, 425
EcoT22I ATGCAT 1 cut(s) 223
FaeI CATG 3 cut(s) 305, 536, 545
FatI CATG 3 cut(s) 301, 532, 541
FokI GGATG 2 cut(s) 87, 566
FspBI CTAG 6 cut(s) 45, 206, 285, 311, 527, 584
HaeIII GGCC 1 cut(s) 540
HapII CCGG 1 cut(s) 448
Hin1II CATG 3 cut(s) 305, 536, 545
HinfI GANTC 5 cut(s) 288, 507, 575, 598, 632
HpaII CCGG 1 cut(s) 448
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 111, 163
Hpy188I TCNGA 3 cut(s) 394, 490, 655
Hpy188III TCNNGA 3 cut(s) 72, 311, 443
Hpy8I GTNNAC 2 cut(s) 111, 163
HpyAV CCTTC 1 cut(s) 495
HpyCH4III ACNGT 3 cut(s) 25, 106, 167
HpyCH4V TGCA 4 cut(s) 215, 221, 369, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 2 cut(s) 53, 337
Hsp92II CATG 3 cut(s) 305, 536, 545
Kzo9I GATC 2 cut(s) 202, 450
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 332
LpnPI CCDG 8 cut(s) 57, 166, 182, 363, 420, 428, 461, 596
LweI GCATC 3 cut(s) 208, 390, 421
MaeI CTAG 6 cut(s) 45, 206, 285, 311, 527, 584
MaeIII GTNAC 3 cut(s) 40, 467, 572
MalI GATC 2 cut(s) 204, 452
MboI GATC 2 cut(s) 202, 450
MboII GAAGA 5 cut(s) 60, 104, 257, 407, 452
MfeI CAATTG 1 cut(s) 479
MluCI AATT 7 cut(s) 58, 315, 407, 479, 513, 545, 625
MlyI GAGTC 2 cut(s) 569, 626
MnlI CCTC 7 cut(s) 88, 92, 112, 180, 254, 298, 514
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 1 cut(s) 80
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 1 cut(s) 448
MunI CAATTG 1 cut(s) 479
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 2 cut(s) 202, 450
NlaIII CATG 3 cut(s) 305, 536, 545
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 2 cut(s) 467, 572
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 536
PciI ACATGT 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 288, 507, 598
PleI GAGTC 2 cut(s) 569, 626
PpsI GAGTC 2 cut(s) 569, 626
PscI ACATGT 1 cut(s) 532
PshAI GACNNNNGTC 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 612
RseI CAYNNNNRTG 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 80
Sau3AI GATC 2 cut(s) 202, 450
Sau96I GGNCC 1 cut(s) 445
SchI GAGTC 2 cut(s) 569, 626
SetI ASST 6 cut(s) 116, 265, 309, 343, 525, 615
SfaNI GCATC 3 cut(s) 208, 390, 421
SfcI CTRYAG 1 cut(s) 608
SinI GGWCC 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 498
Sse9I AATT 7 cut(s) 58, 315, 407, 479, 513, 545, 625
SsiI CCGC 1 cut(s) 475
SspMI CTAG 6 cut(s) 45, 206, 285, 311, 527, 584
TaaI ACNGT 3 cut(s) 25, 106, 167
TaqI TCGA 1 cut(s) 291
TaqII GACCGA 2 cut(s) 32, 273
TasI AATT 7 cut(s) 58, 315, 407, 479, 513, 545, 625
TfiI GAWTC 3 cut(s) 288, 507, 598
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 3 cut(s) 30, 111, 382
TseFI GTSAC 2 cut(s) 467, 572
Tsp45I GTSAC 2 cut(s) 467, 572
TspDTI ATGAA 1 cut(s) 558
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 3 cut(s) 30, 111, 382
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 2 cut(s) 407, 545
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 536
XspI CTAG 6 cut(s) 45, 206, 285, 311, 527, 584
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.