Rh1DG299200

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000555
Physical Location & Seq
Forward (+)
52448146 .. 52453355
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG299200.1

Sequence Viewer

Length: 1218 bp
ATGGCTGCTGCAGCAAATTCTATGCTTGGAAACGGTTGTACACTCTTGGAAAAGGTCGCACGCCTGTTTGTTACTAGGTTTCTAAGACAATATATCTCTACAGGATGTTTCATTTTAATAGAGGAAGACGGCGCAATGTTAACCTTTGAGGGAACGAGCATCGGCTGTTCTTTAAAATCTATTCTTAAAGTTCACAATCCTCAGTTTTACTGGAAGGTAATGACTCGGTCTGATTTAGGCCTTGCAGATGCATACATCAACGGAGATTTTTCTTTCGATGATAAAGACAAAGGTCTTGAGAATCTCTTTTTGATTCTCATTGCCAATAGAGATTCAAATTCTTCCCTCTCAAACATGAGCAAGAAAAGGGGATGGTGGACGCCATTGTTATATGCAGCTAGTATAACCCCAGCACGATATTTCTTTCATCACATTTCAAGGCAAAACACTCTCACACAGGCTCGGAGGAACATCTCTCGCCATTATGACCTGGCTAGAATTAATAAGAACCATGAAGTCCTTGAGATTGGCTGTGGTTGGGGCAGCTTCGCTATTGAAGTGGTCAAGCAAACTGGTTGCAGATACACTGGCATTACTTTGTCCGAGGAACAACTGAAATTTGCCGAAAAGAAAGTGAAAGATGCTGGCCTTCAGGACTACATCAAGTTTCTGTTGTGTGACTACCGCAAACTGCCTCCGAACTACAAGTATGACAGAATTATTTCTTGTGAGATGCTGGAACACGTCGGCCACAAATTTATGGACGAATTCTTTGGTTGCTGTGAATCTCTCCTAGCAGAAAAAGGGCTTCTTGTTCTACAGTTCACATCACTACCGGATGAGCGTTATGATGAGTATAGACAAAGTTCAGACTTCATAAATGAATATATTTTTCCTGGTGCATGTATACCTTCGTTAAGTAGGGTGACATCAGCCATGGCTGCTTCTTCTAGACTCAGTGTGGAGCACTTGGAAAATATTGGAATTCACTATTATCGAACGCTTAAATATTGGAGAAAAAATTTCTTAGAGAAACATGATGAAATACTAGCTCTTGGATTTGATGAAAAATTCATTCGGACGTGGGAGTATTATTTTGATTATTGCGCGGCAGGATTCAACTCATGCACGCTAGGAAATTACCAGATTGTGTTTTCTCATCCTGGTAATGTTCTTGTATTAGGCAACCCCTACAAAGGCTTCCCTTCTGCTTACTGA

Protein Analysis

405

Amino Acids

46.5

Weight (kDa)

7.53

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 147 - 378 1.3e-55 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 164 - 280 2e-11 Methyltransferase domain
Methyltransf_31 PF13847 168 - 281 1.5e-11 Methyltransferase domain
Methyltransf_25 PF13649 173 - 269 8.7e-16 Methyltransferase domain
Methyltransf_11 PF08241 174 - 273 5e-13 Methyltransferase domain
Methyltransf_12 PF08242 174 - 271 5.3e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 907
AccII CGCG 1 cut(s) 1109
AciI CCGC 2 cut(s) 685, 1109
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 8 cut(s) 16, 337, 617, 755, 767, 984, 1021, 1070
AcuI CTGAAG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 1196
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 4 cut(s) 336, 438, 557, 1120
AjiI CACGTC 2 cut(s) 745, 1083
AjnI CCWGG 3 cut(s) 489, 895, 1162
AluBI AGCT 3 cut(s) 398, 546, 1052
AluI AGCT 3 cut(s) 398, 546, 1052
Alw21I GWGCWC 1 cut(s) 969
AoxI GGCC 3 cut(s) 238, 646, 748
ApeKI GCWGC 6 cut(s) 5, 8, 11, 395, 543, 941
ApoI RAATTY 8 cut(s) 16, 337, 617, 755, 767, 984, 1021, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 755, 787
AseI ATTAAT 1 cut(s) 501
Asp700I GAANNNNTTC 1 cut(s) 721
AspLEI GCGC 2 cut(s) 134, 1109
AsuHPI GGTGA 1 cut(s) 937
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 969
BbvI GCAGC 4 cut(s) 23, 407, 555, 928
BccI CCATC 1 cut(s) 366
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 3 cut(s) 491, 897, 1164
BfaI CTAG 7 cut(s) 75, 399, 495, 794, 951, 1049, 1133
BfmI CTRYAG 3 cut(s) 9, 99, 818
BisI GCNGC 7 cut(s) 6, 9, 12, 396, 544, 942, 1110
BlsI GCNGC 7 cut(s) 7, 10, 13, 397, 545, 943, 1111
Bme1390I CCNGG 3 cut(s) 491, 897, 1164
BmgBI CACGTC 2 cut(s) 745, 1083
BmrFI CCNGG 3 cut(s) 491, 897, 1164
BmsI GCATC 4 cut(s) 168, 238, 631, 723
BoxI GACNNNNGTC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 132
BpuEI CTTGAG 2 cut(s) 317, 542
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 603, 936
BsaWI WCCGGW 1 cut(s) 835
Bsc4I CCNNNNNNNGG 1 cut(s) 1196
Bse1I ACTGG 3 cut(s) 215, 577, 592
Bse3DI GCAATG 2 cut(s) 141, 318
BseBI CCWGG 3 cut(s) 491, 897, 1164
BseDI CCNNGG 2 cut(s) 603, 936
BseGI GGATG 4 cut(s) 110, 377, 844, 1159
BseLI CCNNNNNNNGG 1 cut(s) 1196
BseMI GCAATG 2 cut(s) 141, 318
BseMII CTCAG 2 cut(s) 215, 970
BseNI ACTGG 3 cut(s) 215, 577, 592
BseXI GCAGC 4 cut(s) 23, 407, 555, 928
BseYI CCCAGC 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 1109
BshFI GGCC 3 cut(s) 240, 648, 750
BsiHKAI GWGCWC 1 cut(s) 969
BsiSI CCGG 1 cut(s) 836
BslI CCNNNNNNNGG 1 cut(s) 1196
BsnI GGCC 3 cut(s) 240, 648, 750
Bsp1286I GDGCHC 1 cut(s) 969
Bsp1407I TGTACA 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 685, 1109
BspANI GGCC 3 cut(s) 240, 648, 750
BspCNI CTCAG 2 cut(s) 214, 969
BspFNI CGCG 1 cut(s) 1109
BspMAI CTGCAG 1 cut(s) 13
BsrDI GCAATG 2 cut(s) 141, 318
BsrGI TGTACA 1 cut(s) 38
BsrI ACTGG 3 cut(s) 215, 577, 592
BssECI CCNNGG 2 cut(s) 603, 936
BssNAI GTATAC 1 cut(s) 908
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 936
Bst1107I GTATAC 1 cut(s) 908
Bst2UI CCWGG 3 cut(s) 491, 897, 1164
Bst4CI ACNGT 2 cut(s) 35, 822
BstACI GRCGYC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 61, 646, 1130
BstDEI CTNAG 4 cut(s) 83, 201, 956, 1027
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 4 cut(s) 110, 377, 844, 1159
BstFNI CGCG 1 cut(s) 1109
BstHHI GCGC 2 cut(s) 134, 1109
BstMWI GCNNNNNNNGC 2 cut(s) 11, 941
BstNI CCWGG 3 cut(s) 491, 897, 1164
BstNSI RCATGY 1 cut(s) 906
BstPAI GACNNNNGTC 1 cut(s) 291
BstSCI CCNGG 3 cut(s) 489, 895, 1162
BstSFI CTRYAG 3 cut(s) 9, 99, 818
BstUI CGCG 1 cut(s) 1109
BstV1I GCAGC 4 cut(s) 23, 407, 555, 928
BstV2I GAAGAC 1 cut(s) 132
BstZ17I GTATAC 1 cut(s) 908
BsuRI GGCC 3 cut(s) 240, 648, 750
BtgI CCRYGG 1 cut(s) 936
BtrI CACGTC 2 cut(s) 745, 1083
BtsCI GGATG 4 cut(s) 110, 377, 844, 1159
BtsIMutI CAGTG 2 cut(s) 585, 964
Cac8I GCNNGC 3 cut(s) 61, 646, 1130
CfoI GCGC 2 cut(s) 134, 1109
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 39
CviAII CATG 6 cut(s) 355, 512, 903, 937, 1037, 1125
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 4 cut(s) 83, 201, 956, 1027
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 936
Eco147I AGGCCT 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 635
EcoRI GAATTC 2 cut(s) 767, 984
EcoRII CCWGG 3 cut(s) 489, 895, 1162
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 6 cut(s) 358, 515, 906, 940, 1040, 1128
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FatI CATG 6 cut(s) 354, 511, 902, 936, 1036, 1124
FblI GTMKAC 1 cut(s) 907
Fnu4HI GCNGC 7 cut(s) 6, 9, 12, 396, 544, 942, 1110
FokI GGATG 4 cut(s) 117, 384, 851, 1146
Fsp4HI GCNGC 7 cut(s) 6, 9, 12, 396, 544, 942, 1110
FspBI CTAG 7 cut(s) 75, 399, 495, 794, 951, 1049, 1133
GlaI GCGC 2 cut(s) 133, 1108
GluI GCNGC 7 cut(s) 6, 9, 12, 396, 544, 942, 1110
GsaI CCCAGC 1 cut(s) 413
HaeIII GGCC 3 cut(s) 240, 648, 750
HapII CCGG 1 cut(s) 836
HgaI GACGC 1 cut(s) 388
HhaI GCGC 2 cut(s) 134, 1109
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 6 cut(s) 358, 515, 906, 940, 1040, 1128
Hin6I GCGC 2 cut(s) 132, 1107
HinP1I GCGC 2 cut(s) 132, 1107
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 7 cut(s) 223, 301, 313, 332, 785, 954, 1116
HpaI GTTAAC 1 cut(s) 141
HpaII CCGG 1 cut(s) 836
HphI GGTGA 1 cut(s) 937
Hpy166II GTNNAC 6 cut(s) 41, 141, 193, 378, 825, 908
Hpy188I TCNGA 6 cut(s) 232, 465, 604, 699, 871, 1080
Hpy188III TCNNGA 3 cut(s) 296, 653, 951
Hpy8I GTNNAC 6 cut(s) 41, 141, 193, 378, 825, 908
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 4 cut(s) 208, 659, 921, 1215
HpyCH4III ACNGT 2 cut(s) 35, 822
HpyCH4IV ACGT 2 cut(s) 744, 1082
HpyCH4V TGCA 7 cut(s) 11, 245, 251, 395, 579, 902, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 941
HpyF3I CTNAG 4 cut(s) 83, 201, 956, 1027
HpySE526I ACGT 2 cut(s) 744, 1082
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 6 cut(s) 358, 515, 906, 940, 1040, 1128
HspAI GCGC 2 cut(s) 132, 1107
KspAI GTTAAC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 4 cut(s) 23, 407, 555, 928
LweI GCATC 4 cut(s) 168, 238, 631, 723
MaeI CTAG 7 cut(s) 75, 399, 495, 794, 951, 1049, 1133
MaeII ACGT 2 cut(s) 744, 1082
MaeIII GTNAC 3 cut(s) 70, 677, 925
MboII GAAGA 3 cut(s) 137, 333, 939
MhlI GDGCHC 1 cut(s) 969
MlyI GAGTC 2 cut(s) 217, 948
MnlI CCTC 7 cut(s) 115, 142, 210, 356, 459, 598, 705
Mph1103I ATGCAT 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 721
MseI TTAA 7 cut(s) 116, 140, 173, 186, 501, 917, 1005
MspI CCGG 1 cut(s) 836
MspR9I CCNGG 3 cut(s) 491, 897, 1164
MvaI CCWGG 3 cut(s) 491, 897, 1164
MvnI CGCG 1 cut(s) 1109
MwoI GCNNNNNNNGC 2 cut(s) 11, 941
NcoI CCATGG 1 cut(s) 936
NlaIII CATG 6 cut(s) 358, 515, 906, 940, 1040, 1128
NmuCI GTSAC 2 cut(s) 677, 925
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 1 cut(s) 906
PceI AGGCCT 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 5 cut(s) 301, 313, 332, 785, 1116
PflFI GACNNNGTC 1 cut(s) 226
PkrI GCNGC 7 cut(s) 7, 10, 13, 397, 545, 943, 1111
PleI GAGTC 2 cut(s) 217, 948
PpsI GAGTC 2 cut(s) 217, 948
PshAI GACNNNNGTC 1 cut(s) 291
PshBI ATTAAT 1 cut(s) 501
Psp6I CCWGG 3 cut(s) 489, 895, 1162
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 3 cut(s) 489, 895, 1162
PstI CTGCAG 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 226
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SaqAI TTAA 7 cut(s) 116, 140, 173, 186, 501, 917, 1005
SatI GCNGC 7 cut(s) 6, 9, 12, 396, 544, 942, 1110
SchI GAGTC 2 cut(s) 217, 948
ScrFI CCNGG 3 cut(s) 491, 897, 1164
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 4 cut(s) 168, 238, 631, 723
SfcI CTRYAG 3 cut(s) 9, 99, 818
SmlI CTYRAG 2 cut(s) 296, 521
SmoI CTYRAG 2 cut(s) 296, 521
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 2 cut(s) 685, 1109
SspI AATATT 2 cut(s) 979, 1010
SspMI CTAG 7 cut(s) 75, 399, 495, 794, 951, 1049, 1133
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 3 cut(s) 489, 895, 1162
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 2 cut(s) 35, 822
TaiI ACGT 2 cut(s) 747, 1085
TaqI TCGA 2 cut(s) 276, 997
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 1 cut(s) 38
TauI GCSGC 1 cut(s) 1112
TfiI GAWTC 5 cut(s) 301, 313, 332, 785, 1116
Tru1I TTAA 7 cut(s) 116, 140, 173, 186, 501, 917, 1005
Tru9I TTAA 7 cut(s) 116, 140, 173, 186, 501, 917, 1005
TscAI CASTG 2 cut(s) 592, 964
TseFI GTSAC 2 cut(s) 677, 925
TseI GCWGC 6 cut(s) 5, 8, 11, 395, 543, 941
Tsp45I GTSAC 2 cut(s) 677, 925
TspDTI ATGAA 8 cut(s) 100, 416, 528, 865, 897, 1056, 1063, 1080
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 2 cut(s) 592, 964
Tth111I GACNNNGTC 1 cut(s) 226
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 8 cut(s) 16, 337, 617, 755, 767, 984, 1021, 1070
XbaI TCTAGA 1 cut(s) 950
XceI RCATGY 1 cut(s) 906
XmiI GTMKAC 1 cut(s) 907
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 7 cut(s) 75, 399, 495, 794, 951, 1049, 1133
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.