Rorug01G0297000

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000151
Physical Location & Seq
Forward (+)
41190137 .. 41192410
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0297000.1

Sequence Viewer

Length: 783 bp
ATGGCCGAGCACAGTGAGAGCGCCGATGCTAAGGTGGAGCAGACGATGATGGAGAAGATCAGCGAGAAGATCCACGATCATCACGACTCTTCGTCGTCGTCGTCTTCGGATTCCGACACCGAGAAGCCGGCGCCGGCCACGGAGGAGTCGGCGGCCAAGGAGAAGGTGTTCCGTCTCTTCGGAAGGGAGCGTCCTGTTCATCATGTCTTTGGCGGTGGAAAGCCTGCTGATGTGTTTTTGTGGAGGAACAAGAAAATTTCAGCTGGGGTTCTTGGAGGAGCTACTGCCGTTTATGTCCTTTTTGAGTTGCTTCAATACCACTTGCTTACGCTTGTCTGCCACATTCTGGTTGGTACCCTAGCAATCTTGTTCCTGTGGTCCAATGCCCATACTATTATCCACAAGACTCCACCACGTATCCCAGAGGTTTGTCTTCCTGAGGAGCCATTCCTGCAGGTTGCTTCTGAGCTGAGACATCAAATCAACTGGGCTTTTGCACAACTTCATAACATTGCATCTGGAAGGGAGTTGAAGAAATTCCTCATTGTTATTGCTGGCTTGTGGGTTCTTTCCATTGTTGGGAGTTGGTGCAATTTCTTGACCTTGTTTTACATAACTTTTGTTTTGCTGCACACCGTGCCTGTTCTGTATGAGAAGTATGAACACAAGGTTGACCCACTTGCAGAGAAGGCAACAGTTGAGATAAAAAAGCAGTATGGAGTGTTTGATGCCAAGGTTTTGACAAAAGTTCGCGAAACTGTGCTGAAAAACAAGAGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

260

Amino Acids

29.32

Weight (kDa)

7.81

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 76 - 231 9.4e-58 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 445
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 130, 353
AccB7I CCANNNNNTGG 1 cut(s) 346
AccII CGCG 1 cut(s) 753
AciI CCGC 2 cut(s) 152, 213
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 3 cut(s) 3, 135, 153
AcsI RAATTY 2 cut(s) 255, 536
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 2 cut(s) 346, 579
AgsI TTSAA 2 cut(s) 314, 532
AhdI GACNNNNNGTC 1 cut(s) 91
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 263, 281, 469
AluI AGCT 3 cut(s) 263, 281, 469
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 466
AlwI GGATC 1 cut(s) 64
AoxI GGCC 3 cut(s) 3, 135, 153
ApeKI GCWGC 1 cut(s) 628
ApoI RAATTY 2 cut(s) 255, 536
Asp700I GAANNNNTTC 2 cut(s) 167, 536
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 23, 133
AspS9I GGNCC 1 cut(s) 378
AvaII GGWCC 1 cut(s) 378
AxyI CCTNAGG 1 cut(s) 438
BanI GGYRCC 2 cut(s) 130, 353
BbsI GAAGAC 2 cut(s) 96, 425
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 615
BccI CCATC 1 cut(s) 43
BceAI ACGGC 1 cut(s) 272
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 179, 466
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 2 cut(s) 24, 134
BfuAI ACCTGC 1 cut(s) 445
BfuI GTATCC 1 cut(s) 428
BglII AGATCT 1 cut(s) 776
BisI GCNGC 2 cut(s) 153, 629
BlsI GCNGC 2 cut(s) 154, 630
Bme18I GGWCC 1 cut(s) 378
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 3 cut(s) 132, 355, 444
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 3 cut(s) 16, 524, 718
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 2 cut(s) 96, 425
Bpu10I CCTNAGC 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 138, 156, 732
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 579
Bse118I RCCGGY 2 cut(s) 127, 133
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 510
BseDI CCNNGG 3 cut(s) 138, 156, 732
BseLI CCNNNNNNNGG 2 cut(s) 346, 579
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 3 cut(s) 429, 456, 461
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 3 cut(s) 158, 291, 455
BseXI GCAGC 1 cut(s) 615
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 3 cut(s) 5, 137, 155
BshNI GGYRCC 2 cut(s) 130, 353
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 128, 134
BslI CCNNNNNNNGG 2 cut(s) 346, 579
BsmAI GTCTC 2 cut(s) 179, 466
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 5, 137, 155
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 4 cut(s) 57, 69, 76, 776
Bsp68I TCGCGA 1 cut(s) 753
BspACI CCGC 2 cut(s) 152, 213
BspANI GGCC 3 cut(s) 5, 137, 155
BspCNI CTCAG 3 cut(s) 430, 457, 462
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 3 cut(s) 132, 355, 444
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 2 cut(s) 130, 353
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 2 cut(s) 127, 133
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 2 cut(s) 127, 133
BssECI CCNNGG 3 cut(s) 138, 156, 732
BssMI GATC 4 cut(s) 57, 69, 76, 776
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 2 cut(s) 156, 732
Bst4CI ACNGT 4 cut(s) 14, 637, 697, 760
Bst6I CTCTTC 2 cut(s) 94, 182
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 637
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 4 cut(s) 129, 135, 225, 556
BstDEI CTNAG 5 cut(s) 30, 438, 465, 470, 780
BstDSI CCRYGG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 2 cut(s) 24, 134
BstHHI GCGC 2 cut(s) 23, 133
BstKTI GATC 4 cut(s) 60, 72, 79, 779
BstMAI GTCTC 2 cut(s) 179, 466
BstMBI GATC 4 cut(s) 57, 69, 76, 776
BstMWI GCNNNNNNNGC 3 cut(s) 451, 637, 689
BstSFI CTRYAG 1 cut(s) 452
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 615
BstV2I GAAGAC 2 cut(s) 96, 425
BstX2I RGATCY 2 cut(s) 69, 776
BstYI RGATCY 2 cut(s) 69, 776
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 3 cut(s) 5, 137, 155
BtgI CCRYGG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 19
BtuMI TCGCGA 1 cut(s) 753
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 4 cut(s) 129, 135, 225, 556
CfoI GCGC 2 cut(s) 23, 133
Cfr10I RCCGGY 2 cut(s) 127, 133
Cfr13I GGNCC 1 cut(s) 378
CseI GACGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 354
CviAII CATG 1 cut(s) 203
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 5 cut(s) 30, 438, 465, 470, 780
DinI GGCGCC 1 cut(s) 132
DpnI GATC 4 cut(s) 59, 71, 78, 778
DpnII GATC 4 cut(s) 57, 69, 76, 776
DraIII CACNNNGTG 1 cut(s) 637
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 3 cut(s) 3, 135, 153
Eam1104I CTCTTC 2 cut(s) 94, 182
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 2 cut(s) 94, 182
Eco130I CCWWGG 2 cut(s) 156, 732
Eco47I GGWCC 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 156, 732
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 156, 732
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 1 cut(s) 206
FaiI YATR 8 cut(s) 204, 294, 390, 507, 614, 651, 660, 717
FatI CATG 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 153, 629
Fsp4HI GCNGC 2 cut(s) 153, 629
FspBI CTAG 1 cut(s) 359
GlaI GCGC 2 cut(s) 22, 132
GluI GCNGC 2 cut(s) 153, 629
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 24, 134
HaeIII GGCC 3 cut(s) 5, 137, 155
HapII CCGG 2 cut(s) 128, 134
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 23, 133
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 2 cut(s) 21, 131
HinP1I GCGC 2 cut(s) 21, 131
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 4 cut(s) 86, 110, 146, 406
HpaII CCGG 2 cut(s) 128, 134
Hpy166II GTNNAC 1 cut(s) 673
Hpy188I TCNGA 4 cut(s) 109, 115, 182, 466
Hpy188III TCNNGA 5 cut(s) 83, 437, 519, 598, 752
Hpy8I GTNNAC 1 cut(s) 673
Hpy99I CGWCG 3 cut(s) 97, 100, 103
HpyAV CCTTC 4 cut(s) 157, 177, 516, 682
HpyCH4III ACNGT 4 cut(s) 14, 637, 697, 760
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 6 cut(s) 454, 497, 515, 591, 631, 683
HpyF10VI GCNNNNNNNGC 3 cut(s) 451, 637, 689
HpyF3I CTNAG 5 cut(s) 30, 438, 465, 470, 780
HpySE526I ACGT 1 cut(s) 415
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 2 cut(s) 21, 131
KasI GGCGCC 1 cut(s) 130
KpnI GGTACC 1 cut(s) 357
KroI GCCGGC 2 cut(s) 127, 133
KroNI GCCGGC 2 cut(s) 129, 135
Kzo9I GATC 4 cut(s) 57, 69, 76, 776
LmnI GCTCC 4 cut(s) 37, 187, 278, 442
Lsp1109I GCAGC 1 cut(s) 615
LweI GCATC 3 cut(s) 16, 524, 718
MaeI CTAG 1 cut(s) 359
MaeII ACGT 1 cut(s) 415
MalI GATC 4 cut(s) 59, 71, 78, 778
MboI GATC 4 cut(s) 57, 69, 76, 776
MboII GAAGA 7 cut(s) 67, 79, 81, 96, 169, 425, 544
MflI RGATCY 2 cut(s) 69, 776
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 3 cut(s) 255, 536, 592
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 3 cut(s) 80, 155, 400
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 6 cut(s) 136, 237, 269, 418, 433, 551
MroNI GCCGGC 2 cut(s) 127, 133
MroXI GAANNNNTTC 2 cut(s) 167, 536
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 128, 134
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 3 cut(s) 451, 637, 689
NaeI GCCGGC 2 cut(s) 129, 135
NarI GGCGCC 1 cut(s) 131
NdeII GATC 4 cut(s) 57, 69, 76, 776
NgoMIV GCCGGC 2 cut(s) 127, 133
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 132, 355, 444
NmeAIII GCCGAG 1 cut(s) 31
NruI TCGCGA 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 98
PdiI GCCGGC 2 cut(s) 129, 135
PdmI GAANNNNTTC 2 cut(s) 167, 536
PfeI GAWTC 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 2 cut(s) 154, 630
PleI GAGTC 3 cut(s) 80, 154, 400
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 3 cut(s) 80, 154, 400
Ppu21I YACGTR 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 132, 355, 444
PspPI GGNCC 1 cut(s) 378
PstI CTGCAG 1 cut(s) 456
PsuI RGATCY 2 cut(s) 69, 776
PvuII CAGCTG 1 cut(s) 263
RruI TCGCGA 1 cut(s) 753
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SatI GCNGC 2 cut(s) 153, 629
Sau3AI GATC 4 cut(s) 57, 69, 76, 776
Sau96I GGNCC 1 cut(s) 378
SbfI CCTGCAGG 1 cut(s) 456
SchI GAGTC 3 cut(s) 80, 155, 400
SdaI CCTGCAGG 1 cut(s) 456
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 3 cut(s) 16, 524, 718
SfcI CTRYAG 1 cut(s) 452
SfoI GGCGCC 1 cut(s) 132
SinI GGWCC 1 cut(s) 378
Sse8387I CCTGCAGG 1 cut(s) 456
Sse9I AATT 3 cut(s) 255, 536, 592
SsiI CCGC 2 cut(s) 152, 213
SspDI GGCGCC 1 cut(s) 130
SspMI CTAG 1 cut(s) 359
StyI CCWWGG 2 cut(s) 156, 732
TaaI ACNGT 4 cut(s) 14, 637, 697, 760
TaiI ACGT 1 cut(s) 418
TasI AATT 3 cut(s) 255, 536, 592
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 110
TscAI CASTG 1 cut(s) 19
TseI GCWGC 1 cut(s) 628
TspDTI ATGAA 3 cut(s) 188, 494, 675
TspGWI ACGGA 2 cut(s) 155, 161
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 2 cut(s) 255, 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 167, 536
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.