Rroxscaffold_5G00345860

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000151
Physical Location & Seq
Reverse (-)
16418317 .. 16422412
4096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345860.1

Sequence Viewer

Length: 642 bp
ATGGCGTCAGAGGAAATCCGAAATGTTTCTGAAGGATATCCAATGAAAGGTGGAGATGGCCCCGATAGCTATGCCAAGAACTCTACTTACCAGCAATGGAGTCTCGTTAAAGAAGTTGTTACCAAAGGAATTGCAGAAAAGCTTGACATAGACCTTTTGTTACCTTCCAACTCCTTTCACATTGCGGATCTTGGTTGCTCTGTTGGTCCTAATACATTTTCTTCAGTTGAAAACATAGTTGAAGCTGTACAGCTAAAGTTTCAAAGCCAAGGACTCATGAATCACCAAATCCCTGAATTTCAAGTTTTCTTCAATGATCATACCCCAAATGATTTTAACTTGCTCTTCAAATCCCTCCCTTCCAACGAGCAATACTATCTGCGGGAGTTCCGGAGTTCTTTTTACGGGAGTAATTACGAAGAGAAACTAGATTCATTCAACATACCAATGTATTCCGTCCGCCCCCAAGAACTCGAAGCGGTTGTCGAACAAAATGGATCTTTTAGCATAGAGACATTGGAAACAATACCTCATGTTTTAGCTGATGATGCTGTCTTAAATGCAAAACAACTTGCAGCTCATGGAAGAGCCGTCTTGGAGGGACTCATCAAACAGCAATTTGGAAAAGAAATCACGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

213

Amino Acids

23.96

Weight (kDa)

4.81

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 48 - 135 2.2e-25 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 140 - 212 1.2e-15 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 4 cut(s) 185, 382, 460, 479
AclWI GGATC 2 cut(s) 195, 505
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 2 cut(s) 51, 207
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 7 cut(s) 230, 242, 263, 302, 313, 349, 439
AluBI AGCT 6 cut(s) 69, 142, 245, 253, 542, 578
AluI AGCT 6 cut(s) 69, 142, 245, 253, 542, 578
Alw26I GTCTC 2 cut(s) 107, 506
AlwI GGATC 2 cut(s) 195, 505
Aor13HI TCCGGA 1 cut(s) 390
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 468, 500
Asp700I GAANNNNTTC 1 cut(s) 25
AspS9I GGNCC 2 cut(s) 59, 206
AsuHPI GGTGA 1 cut(s) 275
AvaII GGWCC 1 cut(s) 206
BauI CACGAG 1 cut(s) 634
BbvI GCAGC 1 cut(s) 587
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 575
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 2 cut(s) 107, 506
BfaI CTAG 1 cut(s) 428
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 59, 206
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 1 cut(s) 538
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 390
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse3DI GCAATG 2 cut(s) 101, 180
BseAI TCCGGA 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 268
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 2 cut(s) 101, 180
BseXI GCAGC 1 cut(s) 587
BshFI GGCC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 391
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 2 cut(s) 107, 506
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 1 cut(s) 60
Bsp13I TCCGGA 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 247
Bsp143I GATC 3 cut(s) 187, 316, 497
BspACI CCGC 4 cut(s) 185, 382, 460, 479
BspANI GGCC 1 cut(s) 60
BspEI TCCGGA 1 cut(s) 390
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 2 cut(s) 195, 505
BspQI GCTCTTC 2 cut(s) 350, 580
BsrDI GCAATG 2 cut(s) 101, 180
BsrGI TGTACA 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 3 cut(s) 187, 316, 497
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 634
BssT1I CCWWGG 1 cut(s) 268
Bst2BI CACGAG 1 cut(s) 634
Bst6I CTCTTC 3 cut(s) 350, 414, 580
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 247
BstKTI GATC 3 cut(s) 190, 319, 500
BstMAI GTCTC 2 cut(s) 107, 506
BstMBI GATC 3 cut(s) 187, 316, 497
BstMWI GCNNNNNNNGC 2 cut(s) 66, 548
BstV1I GCAGC 1 cut(s) 587
BstX2I RGATCY 2 cut(s) 187, 497
BstYI RGATCY 2 cut(s) 187, 497
BsuRI GGCC 1 cut(s) 60
CciI TCATGA 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 59, 206
Csp6I GTAC 1 cut(s) 248
CviAII CATG 3 cut(s) 277, 533, 581
CviJI RGCY 9 cut(s) 60, 69, 142, 245, 253, 267, 542, 578, 590
CviKI_1 RGCY 9 cut(s) 60, 69, 142, 245, 253, 267, 542, 578, 590
CviQI GTAC 1 cut(s) 248
DpnI GATC 3 cut(s) 189, 318, 499
DpnII GATC 3 cut(s) 187, 316, 497
Eam1104I CTCTTC 3 cut(s) 350, 414, 580
EarI CTCTTC 3 cut(s) 350, 414, 580
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 268
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 51, 207
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 3 cut(s) 280, 536, 584
FaiI YATR 9 cut(s) 72, 149, 236, 278, 321, 443, 509, 534, 582
FaqI GGGAC 1 cut(s) 615
FatI CATG 3 cut(s) 276, 532, 580
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 576
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 1 cut(s) 428
GluI GCNGC 1 cut(s) 576
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 391
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 280, 536, 584
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 5 cut(s) 100, 273, 280, 431, 603
HpaII CCGG 1 cut(s) 391
HphI GGTGA 1 cut(s) 275
Hpy188I TCNGA 3 cut(s) 10, 20, 31
Hpy188III TCNNGA 3 cut(s) 277, 391, 634
HpyAV CCTTC 3 cut(s) 26, 174, 369
HpyCH4V TGCA 3 cut(s) 134, 563, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 548
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 280, 536, 584
Kpn2I TCCGGA 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 3 cut(s) 187, 316, 497
LguI GCTCTTC 2 cut(s) 350, 580
LpnPI CCDG 3 cut(s) 104, 306, 404
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 1 cut(s) 538
MaeI CTAG 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 118, 159
MalI GATC 3 cut(s) 189, 318, 499
MboI GATC 3 cut(s) 187, 316, 497
MboII GAAGA 5 cut(s) 213, 301, 337, 431, 597
MflI RGATCY 2 cut(s) 187, 497
MluCI AATT 4 cut(s) 129, 296, 412, 617
MlyI GAGTC 3 cut(s) 109, 267, 597
MmeI TCCRAC 2 cut(s) 192, 387
MnlI CCTC 4 cut(s) 4, 365, 540, 592
MroI TCCGGA 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 3 cut(s) 108, 336, 557
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 1 cut(s) 391
MwoI GCNNNNNNNGC 2 cut(s) 66, 548
NdeII GATC 3 cut(s) 187, 316, 497
NlaIII CATG 3 cut(s) 280, 536, 584
NlaIV GGNNCC 1 cut(s) 61
PagI TCATGA 1 cut(s) 276
PciSI GCTCTTC 2 cut(s) 350, 580
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 280, 431
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 3 cut(s) 108, 267, 597
PpsI GAGTC 3 cut(s) 108, 267, 597
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 59, 206
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PsuI RGATCY 2 cut(s) 187, 497
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 446
SapI GCTCTTC 2 cut(s) 350, 580
SaqAI TTAA 3 cut(s) 108, 336, 557
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 3 cut(s) 187, 316, 497
Sau96I GGNCC 2 cut(s) 59, 206
SchI GAGTC 3 cut(s) 109, 267, 597
SfaNI GCATC 1 cut(s) 538
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 4 cut(s) 129, 296, 412, 617
SsiI CCGC 4 cut(s) 185, 382, 460, 479
SspMI CTAG 1 cut(s) 428
StyI CCWWGG 1 cut(s) 268
TaqI TCGA 2 cut(s) 474, 486
TasI AATT 4 cut(s) 129, 296, 412, 617
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 2 cut(s) 280, 431
Tru1I TTAA 3 cut(s) 108, 336, 557
Tru9I TTAA 3 cut(s) 108, 336, 557
TseI GCWGC 1 cut(s) 575
TspDTI ATGAA 3 cut(s) 59, 293, 423
TspGWI ACGGA 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.