MD05G1007900.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000151
Physical Location & Seq
Reverse (-)
1807636 .. 1810518
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1007900.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGCAGCAACAGAGGAAAACAGCAAAGTCTGTGAAGCTTATCCAATGAAAGGTGGAAATGGAGCCAACAGCTATGCCAACATCTCCATTTACCAGAGAGGAGGTGCGGAGGCTTCCAAGGAATTTGTAAACAAGGCAATCGCAGAACTTGGCCTGGAAACCTTGTTATCTTCCAAGACCTTTAGAATTGCAGATTTAGGTTGCTCCGTTGGGCCCAGTACATTTTTTTCAGTTGAAAACATAATTCAAGCTCTGCAGTTGAAATATAAAACCCTAGCGTTGAGCTCCCAATTACCTGAGTTTCAGGTTTTCTTTAATGATCTTAACTCAAATGATTTTAATCTGCTCTTCAACTCCCTCTCACACAACAGGCAATACTATGCCGCTGGGGTGCCAGGCTCCTTCTATGGTCGGCTATTTCCTAATAATTCCATCCACCTTTTTTACTCTTCCTTTTCCATTCCATGGATTTCTCGAGTCCCAAAAGAGGTAGTGAATAAAAACAGTCCTGCCTGGAATAAAGGTCGAATCTTTTACTCAGATGCCCCAGATGAAGTAGTAAGGGCTTATGAAGCACAACATGTTGAGGACATGGATTGCTTCCTGAATGCGAGGGCACAAGAAATTGTGAATGGAGGACTCATGGTACTTAATGTTCCAGGTCGCCAAGACGGTACCCCTCATTCTCAAACTCTGCCAAATGTGATCTTTCAAATTTTGGGATCTTGCCTCATGGATATGGCTAGGAAGGGAATTGTTGATGAAGAGAAAGTAGATTCATTTAACCTACCCAATTACTTAATGTCTTCCAAAGAACTAGAAGCTTCTATAGAACGAAATGGATGCTTTAGCTTAGAGAGAAGGGAAAATTTGCATCACTTCGTTGCACATGACATTGTCTATAAAAATCCCCTACTACTTGCATCTCACATCAGAGGTAGCCTGGAGGGACTCATCAAGCAGCATTTTGGAGACGAAATCTTGGACGAGCTCTTTGACTTGTATGGAAAAAGACTTGCAGAGCAACAATCCATTGTTGTGGCAGGGAAGGCAATTGTCTATTCGGTTGTGCTTAGACGCAAGGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

364

Amino Acids

40.57

Weight (kDa)

6.02

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 46 - 360 2.1e-101 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 2 cut(s) 391, 674
AccB7I CCANNNNNTGG 1 cut(s) 465
AciI CCGC 2 cut(s) 107, 384
AclWI GGATC 1 cut(s) 730
AcsI RAATTY 3 cut(s) 122, 714, 868
AfaI GTAC 3 cut(s) 220, 648, 676
AfiI CCNNNNNNNGG 3 cut(s) 50, 465, 487
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 5 cut(s) 236, 248, 262, 352, 713
AjnI CCWGG 5 cut(s) 153, 394, 512, 658, 942
AluBI AGCT 7 cut(s) 38, 72, 251, 285, 824, 852, 991
AluI AGCT 7 cut(s) 38, 72, 251, 285, 824, 852, 991
Alw21I GWGCWC 2 cut(s) 287, 993
Alw26I GTCTC 1 cut(s) 966
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 2 cut(s) 151, 212
ApaI GGGCCC 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 5, 961
ApoI RAATTY 3 cut(s) 122, 714, 868
ArsI GACNNNNNNTTYG 2 cut(s) 977, 1009
Asp718I GGTACC 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 212, 213
AvaI CYCGRG 1 cut(s) 474
BaeGI GKGCMC 2 cut(s) 216, 619
BanI GGYRCC 2 cut(s) 391, 674
BanII GRGCYC 3 cut(s) 216, 287, 993
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 2 cut(s) 287, 993
BbvI GCAGC 2 cut(s) 17, 973
BccI CCATC 1 cut(s) 440
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BciT130I CCWGG 5 cut(s) 155, 396, 514, 660, 944
BcoDI GTCTC 1 cut(s) 966
BfaI CTAG 3 cut(s) 275, 744, 818
BfmI CTRYAG 2 cut(s) 254, 828
BisI GCNGC 3 cut(s) 6, 384, 962
BlsI GCNGC 3 cut(s) 7, 385, 963
Bme1390I CCNGG 5 cut(s) 155, 396, 514, 660, 944
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 2 cut(s) 212, 213
BmiI GGNNCC 5 cut(s) 64, 214, 393, 400, 676
BmrFI CCNGG 5 cut(s) 155, 396, 514, 660, 944
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 4 cut(s) 532, 833, 883, 932
BmuI ACTGGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 798
BpmI CTGGAG 1 cut(s) 965
BsaBI GATNNNNATC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 117, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 465, 487
Bse1I ACTGG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 5 cut(s) 155, 396, 514, 660, 944
BseDI CCNNGG 2 cut(s) 117, 464
BseGI GGATG 2 cut(s) 432, 848
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 3 cut(s) 50, 465, 487
BseMII CTCAG 2 cut(s) 288, 552
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 114
BseSI GKGCMC 2 cut(s) 216, 619
BseXI GCAGC 2 cut(s) 17, 973
BseYI CCCAGC 1 cut(s) 386
BshFI GGCC 2 cut(s) 153, 214
BshNI GGYRCC 2 cut(s) 391, 674
BsiHKAI GWGCWC 2 cut(s) 287, 993
BsiHKCI CYCGRG 1 cut(s) 474
BslFI GGGAC 2 cut(s) 464, 963
BslI CCNNNNNNNGG 3 cut(s) 50, 465, 487
BsmAI GTCTC 1 cut(s) 966
BsmBI CGTCTC 1 cut(s) 966
BsmFI GGGAC 2 cut(s) 464, 963
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 2 cut(s) 153, 214
BsoBI CYCGRG 1 cut(s) 474
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 4 cut(s) 216, 287, 619, 993
Bsp143I GATC 3 cut(s) 319, 705, 722
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 2 cut(s) 107, 384
BspANI GGCC 2 cut(s) 153, 214
BspCNI CTCAG 2 cut(s) 289, 551
BspLI GGNNCC 5 cut(s) 64, 214, 393, 400, 676
BspMAI CTGCAG 1 cut(s) 258
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 353
BspT107I GGYRCC 2 cut(s) 391, 674
BsrI ACTGG 1 cut(s) 216
BssECI CCNNGG 2 cut(s) 117, 464
BssMI GATC 3 cut(s) 319, 705, 722
BssT1I CCWWGG 2 cut(s) 117, 464
Bst2UI CCWGG 5 cut(s) 155, 396, 514, 660, 944
Bst4CI ACNGT 2 cut(s) 506, 674
Bst6I CTCTTC 3 cut(s) 353, 454, 759
BstDEI CTNAG 4 cut(s) 297, 538, 853, 1073
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 432, 848
BstKTI GATC 3 cut(s) 322, 708, 725
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 3 cut(s) 319, 705, 722
BstMWI GCNNNNNNNGC 2 cut(s) 572, 1049
BstNI CCWGG 5 cut(s) 155, 396, 514, 660, 944
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 5 cut(s) 153, 394, 512, 658, 942
BstSFI CTRYAG 2 cut(s) 254, 828
BstSLI GKGCMC 2 cut(s) 216, 619
BstV1I GCAGC 2 cut(s) 17, 973
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 722
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 2 cut(s) 153, 214
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 432, 848
Cfr13I GGNCC 2 cut(s) 212, 213
CseI GACGC 1 cut(s) 1086
Csp6I GTAC 3 cut(s) 219, 647, 675
CviAII CATG 6 cut(s) 465, 581, 592, 643, 733, 890
CviQI GTAC 3 cut(s) 219, 647, 675
DdeI CTNAG 4 cut(s) 297, 538, 853, 1073
DpnI GATC 3 cut(s) 321, 707, 724
DpnII GATC 3 cut(s) 319, 705, 722
Eam1104I CTCTTC 3 cut(s) 353, 454, 759
EarI CTCTTC 3 cut(s) 353, 454, 759
Ecl136II GAGCTC 2 cut(s) 285, 991
Eco130I CCWWGG 2 cut(s) 117, 464
Eco24I GRGCYC 3 cut(s) 216, 287, 993
Eco53kI GAGCTC 2 cut(s) 285, 991
Eco88I CYCGRG 1 cut(s) 474
EcoICRI GAGCTC 2 cut(s) 285, 991
EcoRII CCWGG 5 cut(s) 153, 394, 512, 658, 942
EcoT14I CCWWGG 2 cut(s) 117, 464
EcoT38I GRGCYC 3 cut(s) 216, 287, 993
ErhI CCWWGG 2 cut(s) 117, 464
Esp3I CGTCTC 1 cut(s) 966
FaeI CATG 6 cut(s) 468, 584, 595, 646, 736, 893
FaqI GGGAC 2 cut(s) 464, 963
FatI CATG 6 cut(s) 464, 580, 591, 642, 732, 889
Fnu4HI GCNGC 3 cut(s) 6, 384, 962
FokI GGATG 2 cut(s) 419, 855
FriOI GRGCYC 3 cut(s) 216, 287, 993
Fsp4HI GCNGC 3 cut(s) 6, 384, 962
FspBI CTAG 3 cut(s) 275, 744, 818
GluI GCNGC 3 cut(s) 6, 384, 962
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 965
HaeIII GGCC 2 cut(s) 153, 214
HgaI GACGC 1 cut(s) 1086
Hin1II CATG 6 cut(s) 468, 584, 595, 646, 736, 893
HindIII AAGCTT 2 cut(s) 36, 822
HinfI GANTC 5 cut(s) 477, 528, 639, 776, 951
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 541, 935
Hpy188III TCNNGA 2 cut(s) 474, 604
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 412, 742, 855, 1042
HpyCH4III ACNGT 2 cut(s) 506, 674
HpyCH4V TGCA 6 cut(s) 191, 256, 874, 887, 923, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 1049
HpyF3I CTNAG 4 cut(s) 297, 538, 853, 1073
Hsp92II CATG 6 cut(s) 468, 584, 595, 646, 736, 893
KpnI GGTACC 1 cut(s) 678
Kzo9I GATC 3 cut(s) 319, 705, 722
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 4 cut(s) 62, 209, 290, 404
Lsp1109I GCAGC 2 cut(s) 17, 973
LweI GCATC 4 cut(s) 532, 833, 883, 932
MaeI CTAG 3 cut(s) 275, 744, 818
MalI GATC 3 cut(s) 321, 707, 724
MboI GATC 3 cut(s) 319, 705, 722
MboII GAAGA 5 cut(s) 162, 340, 441, 776, 798
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 4 cut(s) 216, 287, 619, 993
MlyI GAGTC 3 cut(s) 486, 633, 945
MseI TTAA 6 cut(s) 315, 324, 339, 651, 783, 800
MslI CAYNNNNRTG 2 cut(s) 737, 1037
MspA1I CMGCKG 1 cut(s) 386
MspR9I CCNGG 5 cut(s) 155, 396, 514, 660, 944
MunI CAATTG 1 cut(s) 1053
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 5 cut(s) 155, 396, 514, 660, 944
MwoI GCNNNNNNNGC 2 cut(s) 572, 1049
NcoI CCATGG 1 cut(s) 464
NdeII GATC 3 cut(s) 319, 705, 722
NlaIII CATG 6 cut(s) 468, 584, 595, 646, 736, 893
NlaIV GGNNCC 5 cut(s) 64, 214, 393, 400, 676
NspI RCATGY 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 474
PciI ACATGT 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 353
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 528, 776
PflFI GACNNNGTC 1 cut(s) 896
PflMI CCANNNNNTGG 1 cut(s) 465
PkrI GCNGC 3 cut(s) 7, 385, 963
PleI GAGTC 3 cut(s) 485, 633, 945
PpsI GAGTC 3 cut(s) 485, 633, 945
PscI ACATGT 1 cut(s) 580
Psp124BI GAGCTC 2 cut(s) 287, 993
Psp6I CCWGG 5 cut(s) 153, 394, 512, 658, 942
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 5 cut(s) 153, 394, 512, 658, 942
PspN4I GGNNCC 5 cut(s) 64, 214, 393, 400, 676
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 212, 213
PsrI GAACNNNNNNTAC 2 cut(s) 639, 671
PstI CTGCAG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 722
PsyI GACNNNGTC 1 cut(s) 896
RsaI GTAC 3 cut(s) 220, 648, 676
RsaNI GTAC 3 cut(s) 219, 647, 675
RseI CAYNNNNRTG 2 cut(s) 737, 1037
SacI GAGCTC 2 cut(s) 287, 993
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 6 cut(s) 315, 324, 339, 651, 783, 800
SatI GCNGC 3 cut(s) 6, 384, 962
Sau3AI GATC 3 cut(s) 319, 705, 722
Sau96I GGNCC 2 cut(s) 212, 213
SchI GAGTC 3 cut(s) 486, 633, 945
ScrFI CCNGG 5 cut(s) 155, 396, 514, 660, 944
SduI GDGCHC 4 cut(s) 216, 287, 619, 993
SfaNI GCATC 4 cut(s) 532, 833, 883, 932
SfcI CTRYAG 2 cut(s) 254, 828
Sfr274I CTCGAG 1 cut(s) 474
SlaI CTCGAG 1 cut(s) 474
SmiMI CAYNNNNRTG 2 cut(s) 737, 1037
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
SsiI CCGC 2 cut(s) 107, 384
SspMI CTAG 3 cut(s) 275, 744, 818
SstI GAGCTC 2 cut(s) 287, 993
StyD4I CCNGG 5 cut(s) 153, 394, 512, 658, 942
StyI CCWWGG 2 cut(s) 117, 464
TaaI ACNGT 2 cut(s) 506, 674
TaqI TCGA 2 cut(s) 475, 526
TatI WGTACW 1 cut(s) 218
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 2 cut(s) 528, 776
Tru1I TTAA 6 cut(s) 315, 324, 339, 651, 783, 800
Tru9I TTAA 6 cut(s) 315, 324, 339, 651, 783, 800
TseI GCWGC 2 cut(s) 5, 961
TspDTI ATGAA 5 cut(s) 62, 567, 585, 768, 777
TspGWI ACGGA 1 cut(s) 196
Tth111I GACNNNGTC 1 cut(s) 896
Van91I CCANNNNNTGG 1 cut(s) 465
XapI RAATTY 3 cut(s) 122, 714, 868
XceI RCATGY 1 cut(s) 584
XhoI CTCGAG 1 cut(s) 474
XspI CTAG 3 cut(s) 275, 744, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.