Rroxscaffold_6G00396060

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000151
Physical Location & Seq
Forward (+)
17303775 .. 17305976
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396060.1

Sequence Viewer

Length: 1092 bp
ATGGCAGCGGAGGAAACTGGTAAATGCTCTGAAGCTTATCCAATGAAAGGTGGAGATGGCCCCAGCAACTATGCCAAGAACTCCATCTACCAGAAAGGAGTAATTGATGCTGCCAAAGAACTTCTAAATAAGGCGATTGCAGAAAAGCTTGACATGGAAACATTTTCATCTGCCAACTCCTTTCACATTGCAGATTTGGGTTGCTCAGTTGGGCCTAATACATTTTTCGCAGTTGAAAACATACTTGAAGCTGTTCTATTCAAGTATCAAAGCCGAGGGCTGAATTGTCAAATCCCTGAATTTCAAGTCTTCTTTAATGATCATACCTCAAATGACTTCAACATGCTCTTCAATTCCCTCCCTCAGAATCGGCAATACTATGCTGCGGGTGTGCCTGGTTCTTTCTATGGTCGAATCCTCCCCGATGCTTCCATCCACTTTTTTCACTCTACTTTTTCCCTTCATTGGCTTTCTAGAGTACCAAAAGATGTAACAGACCGCAACTCCCCTGCTTGGAATAAAGGACGAATACATTACTTAGACTCCACAGATGAAGTAGTGAGGGCTTACGAAGCCCAATATGCCGAGGACATGGAGTGCTTTCTGCATGCCAGGGCACAAGAGACAGTACATGGAGGATTAATGGTACTTGCTACTGCTGGTTACCCAGCTGATACACCTCCTTCTCATTCTCTGGCAAATGTCACCTATCAAATTTTAGGATCTTGCCTCATTGACATGTCTAGGAAGGGAGTAGTCAGCGAGGAGGAAATAGATTCATTTAATGTACCTATATACTATGTGTGTCCCCGAGAACTGGAAGCTGCTGTAGAACGAAATGGATGTTTTAGCATAGAGATAATGGAACACTTACCTACTGTGATGGAACCTGACACTATTTCAAAAAATGGCAAACTCTTTGCATCTCATATGAGAGCTGCCATGGAGGGACTCTTCAAGCAGCATTTCGGAGAAGAAATCTTAGACGAGCTCTTCGACTTGTTTCACAAGAAAGTTGAAGAGCAGCACTTCGCATTTGAGTCAGGGAAGGCAGGGGACATCTTTATTGTGCTTAAACGCAAGGCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

363

Amino Acids

40.71

Weight (kDa)

5.25

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 360 7.2e-107 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 8, 386, 499
AclWI GGATC 1 cut(s) 730
AcsI RAATTY 2 cut(s) 299, 714
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 4 cut(s) 480, 630, 648, 789
AfiI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 9 cut(s) 236, 248, 262, 305, 340, 352, 903, 958, 1019
AjnI CCWGG 2 cut(s) 394, 611
AluBI AGCT 7 cut(s) 35, 148, 251, 671, 824, 938, 991
AluI AGCT 7 cut(s) 35, 148, 251, 671, 824, 938, 991
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 2 cut(s) 58, 212
ApeKI GCWGC 7 cut(s) 5, 110, 383, 824, 938, 961, 1024
ApoI RAATTY 2 cut(s) 299, 714
ArsI GACNNNNNNTTYG 2 cut(s) 977, 1009
AseI ATTAAT 1 cut(s) 641
Asp700I GAANNNNTTC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 59, 212
AsuHPI GGTGA 1 cut(s) 697
AvaI CYCGRG 1 cut(s) 810
BaeGI GKGCMC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 993
BbvI GCAGC 7 cut(s) 17, 97, 370, 811, 925, 973, 1036
BccI CCATC 4 cut(s) 50, 92, 440, 877
BciT130I CCWGG 2 cut(s) 396, 613
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 2 cut(s) 474, 744
BfmI CTRYAG 1 cut(s) 828
BisI GCNGC 7 cut(s) 6, 111, 384, 825, 939, 962, 1025
BlsI GCNGC 7 cut(s) 7, 112, 385, 826, 940, 963, 1026
Bme1390I CCNGG 2 cut(s) 396, 613
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 2 cut(s) 59, 212
BmiI GGNNCC 2 cut(s) 61, 888
BmrFI CCNGG 2 cut(s) 396, 613
BmsI GCATC 3 cut(s) 97, 415, 932
BpiI GAAGAC 1 cut(s) 301
BsaJI CCNNGG 4 cut(s) 274, 585, 612, 942
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 527, 557
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
Bse1I ACTGG 2 cut(s) 22, 822
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 396, 613
BseDI CCNNGG 4 cut(s) 274, 585, 612, 942
BseGI GGATG 2 cut(s) 432, 848
BseLI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 219, 377
BseNI ACTGG 2 cut(s) 22, 822
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 7 cut(s) 17, 97, 370, 811, 925, 973, 1036
BseYI CCCAGC 2 cut(s) 62, 667
BshFI GGCC 2 cut(s) 60, 214
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 1 cut(s) 810
BslFI GGGAC 3 cut(s) 792, 963, 1070
BslI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
BsmAI GTCTC 1 cut(s) 617
BsmFI GGGAC 3 cut(s) 792, 963, 1070
BsnI GGCC 2 cut(s) 60, 214
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 619, 993
Bsp143I GATC 2 cut(s) 319, 722
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 3 cut(s) 8, 386, 499
BspANI GGCC 2 cut(s) 60, 214
BspCNI CTCAG 2 cut(s) 218, 376
BspLI GGNNCC 2 cut(s) 61, 888
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 3 cut(s) 353, 998, 1014
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 2 cut(s) 22, 822
BssECI CCNNGG 4 cut(s) 274, 585, 612, 942
BssMI GATC 2 cut(s) 319, 722
BssT1I CCWWGG 1 cut(s) 942
Bst2UI CCWGG 2 cut(s) 396, 613
Bst4CI ACNGT 2 cut(s) 628, 880
Bst6I CTCTTC 4 cut(s) 353, 959, 998, 1014
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 4 cut(s) 205, 363, 538, 982
BstDSI CCRYGG 1 cut(s) 942
BstEII GGTNACC 1 cut(s) 662
BstF5I GGATG 2 cut(s) 432, 848
BstKTI GATC 2 cut(s) 322, 725
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 319, 722
BstMWI GCNNNNNNNGC 2 cut(s) 572, 581
BstNI CCWGG 2 cut(s) 396, 613
BstNSI RCATGY 3 cut(s) 346, 611, 742
BstPI GGTNACC 1 cut(s) 662
BstSCI CCNGG 2 cut(s) 394, 611
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 7 cut(s) 17, 97, 370, 811, 925, 973, 1036
BstV2I GAAGAC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 2 cut(s) 60, 214
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 432, 848
Cac8I GCNNGC 1 cut(s) 609
Cfr13I GGNCC 2 cut(s) 59, 212
Csp6I GTAC 4 cut(s) 479, 629, 647, 788
CviAII CATG 7 cut(s) 154, 343, 592, 608, 632, 739, 943
CviQI GTAC 4 cut(s) 479, 629, 647, 788
DdeI CTNAG 4 cut(s) 205, 363, 538, 982
DpnI GATC 2 cut(s) 321, 724
DpnII GATC 2 cut(s) 319, 722
Eam1104I CTCTTC 4 cut(s) 353, 959, 998, 1014
EarI CTCTTC 4 cut(s) 353, 959, 998, 1014
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 993
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 810
Eco91I GGTNACC 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 394, 611
EcoT14I CCWWGG 1 cut(s) 942
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 942
FaeI CATG 7 cut(s) 157, 346, 595, 611, 635, 742, 946
FaqI GGGAC 3 cut(s) 792, 963, 1070
FatI CATG 7 cut(s) 153, 342, 591, 607, 631, 738, 942
FauI CCCGC 1 cut(s) 379
FauNDI CATATG 1 cut(s) 930
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 7 cut(s) 6, 111, 384, 825, 939, 962, 1025
FokI GGATG 2 cut(s) 419, 855
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 7 cut(s) 6, 111, 384, 825, 939, 962, 1025
FspBI CTAG 2 cut(s) 474, 744
GluI GCNGC 7 cut(s) 6, 111, 384, 825, 939, 962, 1025
GsaI CCCAGC 2 cut(s) 66, 671
HaeIII GGCC 2 cut(s) 60, 214
Hin1II CATG 7 cut(s) 157, 346, 595, 611, 635, 742, 946
HindIII AAGCTT 2 cut(s) 33, 146
HinfI GANTC 6 cut(s) 367, 414, 542, 776, 951, 1040
HphI GGTGA 1 cut(s) 697
Hpy188I TCNGA 3 cut(s) 31, 366, 971
Hpy188III TCNNGA 1 cut(s) 474
HpyAV CCTTC 4 cut(s) 470, 693, 742, 1042
HpyCH4III ACNGT 2 cut(s) 628, 880
HpyCH4V TGCA 4 cut(s) 140, 191, 607, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 581
HpyF3I CTNAG 4 cut(s) 205, 363, 538, 982
Hsp92II CATG 7 cut(s) 157, 346, 595, 611, 635, 742, 946
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 2 cut(s) 319, 722
LguI GCTCTTC 3 cut(s) 353, 998, 1014
Lsp1109I GCAGC 7 cut(s) 17, 97, 370, 811, 925, 973, 1036
LweI GCATC 3 cut(s) 97, 415, 932
MaeI CTAG 2 cut(s) 474, 744
MaeIII GTNAC 3 cut(s) 490, 662, 703
MalI GATC 2 cut(s) 321, 724
MboI GATC 2 cut(s) 319, 722
MboII GAAGA 6 cut(s) 301, 340, 946, 985, 986, 1031
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 2 cut(s) 619, 993
MluCI AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
MlyI GAGTC 3 cut(s) 536, 945, 1049
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 4 cut(s) 315, 641, 783, 1074
MslI CAYNNNNRTG 1 cut(s) 737
MspA1I CMGCKG 2 cut(s) 8, 671
MspR9I CCNGG 2 cut(s) 396, 613
MvaI CCWGG 2 cut(s) 396, 613
MwoI GCNNNNNNNGC 2 cut(s) 572, 581
NcoI CCATGG 1 cut(s) 942
NdeI CATATG 1 cut(s) 930
NdeII GATC 2 cut(s) 319, 722
NlaIII CATG 7 cut(s) 157, 346, 595, 611, 635, 742, 946
NlaIV GGNNCC 2 cut(s) 61, 888
NmeAIII GCCGAG 2 cut(s) 299, 610
NmuCI GTSAC 1 cut(s) 703
NspI RCATGY 3 cut(s) 346, 611, 742
PaeI GCATGC 1 cut(s) 611
PciI ACATGT 1 cut(s) 738
PciSI GCTCTTC 3 cut(s) 353, 998, 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 3 cut(s) 367, 414, 776
PkrI GCNGC 7 cut(s) 7, 112, 385, 826, 940, 963, 1026
PleI GAGTC 3 cut(s) 536, 945, 1048
PpsI GAGTC 3 cut(s) 536, 945, 1048
PscI ACATGT 1 cut(s) 738
PshBI ATTAAT 1 cut(s) 641
Psp124BI GAGCTC 1 cut(s) 993
Psp6I CCWGG 2 cut(s) 394, 611
PspEI GGTNACC 1 cut(s) 662
PspFI CCCAGC 2 cut(s) 62, 667
PspGI CCWGG 2 cut(s) 394, 611
PspN4I GGNNCC 2 cut(s) 61, 888
PspPI GGNCC 2 cut(s) 59, 212
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PsuI RGATCY 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 4 cut(s) 480, 630, 648, 789
RsaNI GTAC 4 cut(s) 479, 629, 647, 788
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 993
SapI GCTCTTC 3 cut(s) 353, 998, 1014
SaqAI TTAA 4 cut(s) 315, 641, 783, 1074
SatI GCNGC 7 cut(s) 6, 111, 384, 825, 939, 962, 1025
Sau3AI GATC 2 cut(s) 319, 722
Sau96I GGNCC 2 cut(s) 59, 212
SchI GAGTC 3 cut(s) 536, 945, 1049
ScrFI CCNGG 2 cut(s) 396, 613
SduI GDGCHC 2 cut(s) 619, 993
SfaNI GCATC 3 cut(s) 97, 415, 932
SfcI CTRYAG 1 cut(s) 828
SmiMI CAYNNNNRTG 1 cut(s) 737
SphI GCATGC 1 cut(s) 611
Sse9I AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
SsiI CCGC 3 cut(s) 8, 386, 499
SspMI CTAG 2 cut(s) 474, 744
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 2 cut(s) 394, 611
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 2 cut(s) 628, 880
TaqI TCGA 2 cut(s) 412, 996
TasI AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 3 cut(s) 367, 414, 776
Tru1I TTAA 4 cut(s) 315, 641, 783, 1074
Tru9I TTAA 4 cut(s) 315, 641, 783, 1074
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 7 cut(s) 5, 110, 383, 824, 938, 961, 1024
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 5 cut(s) 59, 156, 452, 567, 768
VspI ATTAAT 1 cut(s) 641
XapI RAATTY 2 cut(s) 299, 714
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 3 cut(s) 346, 611, 742
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 2 cut(s) 474, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.