Rh4CG113700

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000151
Physical Location & Seq
Reverse (-)
21570335 .. 21573621
3287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG113700.1

Sequence Viewer

Length: 807 bp
ATGGCTTCACAGGAAATCAGAAAAGTCTCTGAAGCATATCCAATGAAAGGTGGAGATGGCCCCGATAGCTATGCCAAGAACTCTACTTATCAGAGAGAAGCTATGGAGCCTGTCAAAGAACTTGTAACCAAAGCAATTGCAGAAAAGCTTGACATAGACCTTTTGTTACCTTCCAACTCCTTTCACATTGCGGATCTTGGTTGCTCTGTTGGTCCTAATACATTTTCTTCAGTTGAAAACATACTTGAAGCTGTGGAGCTCAAGTTTCAAGGCCAAGGACTCATGAATCCCCAAATCCCCGAATTTCAAGTTTTCTTCAATGATCATACCCCAAATGATTTTAACTTGCTCTTCAAATCCCTCCCTCCCAACAGGCAATACTATGCTGTGGGAGTTCCAGGTTCTTTCTACGGCCGCCTATTCCCTAGTGCTTCAATTCACTTGTTTCACTCTTCTTTTGCTCTTCAATGGCTTTCTGAAGTACCAAAAGATGTAGAGGACAAAAACTGTCCGGCTTGGAATAAAGGACGGATTCATTACCTCAGTTCGACTGATGAAGTAGTAAGGGCTTATAAAGCTCAATATGCTAAGAACATGGAGTCCTTTCTGCATGCTAGGGCACAAGAACTTGTGTATGGAGGACTAATGGTACTTATCATTCCTGGCTACCCACAGGGTACCCCTCTTTCTCATTCTGTGGCATATTTGACCTTACAACTCATAGAAGCCTGTCTAATTGACATGGTTAAAAAGGTAACAAATTTGAACTCTCTAAGCTCAACATTCTACTACTATGTATATAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

268

Amino Acids

30.16

Weight (kDa)

5.98

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 252 6.6e-74 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 677
AciI CCGC 2 cut(s) 191, 415
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 302, 760
AcuI CTGAAG 3 cut(s) 51, 213, 498
AfaI GTAC 3 cut(s) 483, 651, 679
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 9 cut(s) 236, 248, 269, 308, 319, 355, 435, 467, 766
AjnI CCWGG 2 cut(s) 397, 661
AloI GAACNNNNNNTCC 2 cut(s) 584, 616
AluBI AGCT 7 cut(s) 69, 101, 148, 251, 259, 578, 777
AluI AGCT 7 cut(s) 69, 101, 148, 251, 259, 578, 777
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 1 cut(s) 201
AoxI GGCC 3 cut(s) 58, 271, 412
ApoI RAATTY 2 cut(s) 302, 760
Asp718I GGTACC 1 cut(s) 677
AspS9I GGNCC 2 cut(s) 59, 212
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 261
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 2 cut(s) 399, 663
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 2 cut(s) 426, 615
BisI GCNGC 1 cut(s) 415
BlsI GCNGC 1 cut(s) 416
Bme1390I CCNGG 2 cut(s) 399, 663
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 59, 212
BmiI GGNNCC 3 cut(s) 61, 108, 679
BmrFI CCNGG 2 cut(s) 399, 663
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 399, 663
BseDI CCNNGG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 556
BseSI GKGCMC 1 cut(s) 622
BseX3I CGGCCG 1 cut(s) 412
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 3 cut(s) 60, 273, 414
BshNI GGYRCC 1 cut(s) 677
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 3 cut(s) 60, 273, 414
Bsp1286I GDGCHC 2 cut(s) 261, 622
Bsp143I GATC 2 cut(s) 193, 322
BspACI CCGC 2 cut(s) 191, 415
BspANI GGCC 3 cut(s) 60, 273, 414
BspCNI CTCAG 1 cut(s) 555
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 3 cut(s) 61, 108, 679
BspPI GGATC 1 cut(s) 201
BspQI GCTCTTC 2 cut(s) 356, 468
BspT107I GGYRCC 1 cut(s) 677
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 2 cut(s) 193, 322
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 399, 663
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 3 cut(s) 356, 457, 468
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 3 cut(s) 542, 588, 773
BstKTI GATC 2 cut(s) 196, 325
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 2 cut(s) 193, 322
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 66, 575, 584
BstNI CCWGG 2 cut(s) 399, 663
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 2 cut(s) 397, 661
BstSLI GKGCMC 1 cut(s) 622
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BstZI CGGCCG 1 cut(s) 412
BsuRI GGCC 3 cut(s) 60, 273, 414
Cac8I GCNNGC 1 cut(s) 612
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 59, 212
Csp6I GTAC 3 cut(s) 482, 650, 678
CviAII CATG 4 cut(s) 283, 595, 611, 742
CviQI GTAC 3 cut(s) 482, 650, 678
DdeI CTNAG 3 cut(s) 542, 588, 773
DpnI GATC 2 cut(s) 195, 324
DpnII GATC 2 cut(s) 193, 322
EaeI YGGCCR 1 cut(s) 412
EagI CGGCCG 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 356, 457, 468
EarI CTCTTC 3 cut(s) 356, 457, 468
Ecl136II GAGCTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 212
Eco52I CGGCCG 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 3 cut(s) 51, 213, 498
EcoICRI GAGCTC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 397, 661
EcoT14I CCWWGG 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 4 cut(s) 286, 598, 614, 745
FatI CATG 4 cut(s) 282, 594, 610, 741
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 415
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 1 cut(s) 415
FspBI CTAG 2 cut(s) 426, 615
GluI GCNGC 1 cut(s) 415
HaeIII GGCC 3 cut(s) 60, 273, 414
HapII CCGG 1 cut(s) 512
Hin1II CATG 4 cut(s) 286, 598, 614, 745
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 4 cut(s) 279, 286, 532, 599
HpaII CCGG 1 cut(s) 512
Hpy188I TCNGA 4 cut(s) 20, 31, 93, 478
Hpy188III TCNNGA 1 cut(s) 283
HpyAV CCTTC 1 cut(s) 180
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4V TGCA 2 cut(s) 140, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 575, 584
HpyF3I CTNAG 3 cut(s) 542, 588, 773
Hsp92II CATG 4 cut(s) 286, 598, 614, 745
KpnI GGTACC 1 cut(s) 681
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 193, 322
LguI GCTCTTC 2 cut(s) 356, 468
LmnI GCTCC 2 cut(s) 106, 256
LpnPI CCDG 9 cut(s) 123, 358, 384, 411, 525, 648, 659, 675, 742
MaeI CTAG 2 cut(s) 426, 615
MaeIII GTNAC 3 cut(s) 124, 165, 754
MalI GATC 2 cut(s) 195, 324
MboI GATC 2 cut(s) 193, 322
MboII GAAGA 5 cut(s) 219, 307, 343, 444, 455
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 261, 622
MluCI AATT 5 cut(s) 135, 302, 435, 735, 760
MlyI GAGTC 2 cut(s) 273, 608
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 6 cut(s) 371, 375, 490, 551, 632, 693
MseI TTAA 2 cut(s) 342, 747
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 399, 663
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 2 cut(s) 399, 663
MwoI GCNNNNNNNGC 3 cut(s) 66, 575, 584
NdeII GATC 2 cut(s) 193, 322
NlaIII CATG 4 cut(s) 286, 598, 614, 745
NlaIV GGNNCC 3 cut(s) 61, 108, 679
NspI RCATGY 1 cut(s) 614
PaeI GCATGC 1 cut(s) 614
PagI TCATGA 1 cut(s) 282
PciSI GCTCTTC 2 cut(s) 356, 468
PfeI GAWTC 2 cut(s) 286, 532
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 2 cut(s) 273, 607
PpsI GAGTC 2 cut(s) 273, 607
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 397, 661
PspGI CCWGG 2 cut(s) 397, 661
PspN4I GGNNCC 3 cut(s) 61, 108, 679
PspPI GGNCC 2 cut(s) 59, 212
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 3 cut(s) 483, 651, 679
RsaNI GTAC 3 cut(s) 482, 650, 678
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 2 cut(s) 356, 468
SaqAI TTAA 2 cut(s) 342, 747
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 2 cut(s) 193, 322
Sau96I GGNCC 2 cut(s) 59, 212
SchI GAGTC 2 cut(s) 273, 608
ScrFI CCNGG 2 cut(s) 399, 663
SduI GDGCHC 2 cut(s) 261, 622
SinI GGWCC 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SphI GCATGC 1 cut(s) 614
Sse9I AATT 5 cut(s) 135, 302, 435, 735, 760
SsiI CCGC 2 cut(s) 191, 415
SspMI CTAG 2 cut(s) 426, 615
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 397, 661
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 509
TaqI TCGA 1 cut(s) 548
TasI AATT 5 cut(s) 135, 302, 435, 735, 760
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 2 cut(s) 286, 532
Tru1I TTAA 2 cut(s) 342, 747
Tru9I TTAA 2 cut(s) 342, 747
TspDTI ATGAA 4 cut(s) 59, 299, 524, 570
TspGWI ACGGA 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 2 cut(s) 302, 760
XceI RCATGY 1 cut(s) 614
XspI CTAG 2 cut(s) 426, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.