MD09G1254300.v1.1

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000151
Physical Location & Seq
Forward (+)
32572614 .. 32578134
5521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1254300.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGCAGCTGCAGTGGAGGATTCCAGTAATGTTTCTGAAGCCTACCCAATGACAGGCGGAGATGGCCCCAACAGCTATGCCAAGAACTCGATACGCCAGAAACTAGTTGTTGATGCTGCCAAAGAACTTCTAACCAAGACTATTAAACAAAATCTTGATCTGGACATCTGCTTACCTTGCGACACCTTCCATATTGCAGATTTGGGTTGCTCGGTTGGGCCTAATACTTTTTTATCGGTTGAAAACATACTTGAAGCTGTGAAATTCGAGTATCAAAGCCGAGAGCTAAGTTCCCAAATCCCCGAATTTCAAGCTTTTTTCAATGATCACACCCTAAATGATTTTAACATGCTCTTCAAATCCCTCCCTCGGAACAGGAACTACTATGCAGCGGGTGTGCCAGGTTCCTTCTACGGTCGCTTATTTCCTAAGGCTTCCATTCACTTTTTTCACTCGTCGTTAGCCGTTCATTGGCTTTCTAGAGTCCCAAAAGATGTAACGGAGAAAACCAGTCGAGCTTGGAATAAAGGAAGAATCCACTACTCAAATTCCCGAGACGAAGTGGTCAAGGCTTATCAAGCCCAATATTCTGAGGACATGGAGTGTTTCCTCCATGCCAGGGCGCTAGAGACTGTGCATCGAGGATTGGTAGTGCTTACCCTTTTAGGACACCACAATGGTGATTCTCACTCTCTAGGAAATGTGACTGTTGACATTTTAGGATCTTGCCTCATGGACATGGCTAGGAAGGGAGTAGTTAGTGAAGAGAAGGTAGATTCATTTAACATCCCTTCATACTGCGTTTCTCCTCAAGAACTGGAAGTCATTGTAGAACGAAATGGATGCTTCAGCATAGAGGCTTTGGAAACCCTAGTTTATCATGTCCCGGAACATGGCGCTAAACGTAGTGCATCTACGATGAGAGCTGCCACAGAGGGACTCATCAAGCAACAATTTGGAGAAGAAATCTTAGATGAACTCTTCAGCTTGTATCAGCAAAAAATTGATGAGCAAATCTCAATATTTGAGTCAAAGAAGTCGGTTATCTTTTTTGTTGTGCTTAAACGCAAGACGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

360

Amino Acids

40.33

Weight (kDa)

5.92

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 356 9.6e-106 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 563
AciI CCGC 2 cut(s) 57, 392
AclWI GGATC 1 cut(s) 730
AcsI RAATTY 3 cut(s) 263, 305, 547
AcuI CTGAAG 3 cut(s) 57, 832, 967
AfiI CCNNNNNNNGG 5 cut(s) 53, 369, 471, 619, 893
AgsI TTSAA 5 cut(s) 242, 254, 311, 322, 358
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 2 cut(s) 400, 617
AleI CACNNNNGTG 1 cut(s) 678
AluBI AGCT 8 cut(s) 8, 75, 257, 286, 314, 518, 926, 987
AluI AGCT 8 cut(s) 8, 75, 257, 286, 314, 518, 926, 987
Alw26I GTCTC 2 cut(s) 549, 623
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 2 cut(s) 64, 218
ApeKI GCWGC 5 cut(s) 5, 8, 116, 389, 926
ApoI RAATTY 3 cut(s) 263, 305, 547
AspLEI GCGC 2 cut(s) 625, 899
AspS9I GGNCC 2 cut(s) 65, 218
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 552
AxyI CCTNAGG 1 cut(s) 429
BbvI GCAGC 4 cut(s) 17, 103, 401, 913
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 449
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BciT130I CCWGG 2 cut(s) 402, 619
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 1 cut(s) 887
BcoDI GTCTC 2 cut(s) 549, 623
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 6 cut(s) 104, 480, 626, 695, 744, 872
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 2 cut(s) 626, 900
BisI GCNGC 5 cut(s) 6, 9, 117, 390, 927
BlsI GCNGC 5 cut(s) 7, 10, 118, 391, 928
Bme1390I CCNGG 3 cut(s) 402, 619, 887
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 65, 218
BmiI GGNNCC 2 cut(s) 67, 406
BmrFI CCNGG 3 cut(s) 402, 619, 887
BmsI GCATC 4 cut(s) 103, 646, 833, 920
BplI GAGNNNNNCTC 2 cut(s) 1001, 1033
BpuEI CTTGAG 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 887
BsaJI CCNNGG 2 cut(s) 368, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 369, 471, 619, 893
Bse1I ACTGG 3 cut(s) 24, 510, 822
Bse21I CCTNAGG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 402, 619
BseDI CCNNGG 2 cut(s) 368, 618
BseGI GGATG 2 cut(s) 786, 848
BseLI CCNNNNNNNGG 5 cut(s) 53, 369, 471, 619, 893
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 24, 510, 822
BseRI GAGGAG 1 cut(s) 798
BseXI GCAGC 4 cut(s) 17, 103, 401, 913
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 2 cut(s) 66, 220
BsiEI CGRYCG 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 1 cut(s) 887
BslFI GGGAC 3 cut(s) 470, 869, 951
BslI CCNNNNNNNGG 5 cut(s) 53, 369, 471, 619, 893
BsmAI GTCTC 2 cut(s) 549, 623
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 3 cut(s) 470, 869, 951
BsnI GGCC 2 cut(s) 66, 220
BsoBI CYCGRG 1 cut(s) 552
Bsp143I GATC 3 cut(s) 157, 325, 722
BspACI CCGC 2 cut(s) 57, 392
BspANI GGCC 2 cut(s) 66, 220
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 2 cut(s) 67, 406
BspMAI CTGCAG 1 cut(s) 13
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 359
BsrI ACTGG 3 cut(s) 24, 510, 822
BssECI CCNNGG 2 cut(s) 368, 618
BssMI GATC 3 cut(s) 157, 325, 722
Bst2UI CCWGG 2 cut(s) 402, 619
Bst4CI ACNGT 3 cut(s) 416, 634, 709
Bst6I CTCTTC 3 cut(s) 359, 759, 986
BstDEI CTNAG 4 cut(s) 287, 429, 591, 970
BstF5I GGATG 2 cut(s) 786, 848
BstH2I RGCGCY 2 cut(s) 626, 900
BstHHI GCGC 2 cut(s) 625, 899
BstKTI GATC 3 cut(s) 160, 328, 725
BstMAI GTCTC 2 cut(s) 549, 623
BstMBI GATC 3 cut(s) 157, 325, 722
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 4 cut(s) 63, 72, 177, 578
BstNI CCWGG 2 cut(s) 402, 619
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 400, 617, 885
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 4 cut(s) 17, 103, 401, 913
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
Bsu36I CCTNAGG 1 cut(s) 429
BsuRI GGCC 2 cut(s) 66, 220
BtsCI GGATG 2 cut(s) 786, 848
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
CfoI GCGC 2 cut(s) 625, 899
Cfr13I GGNCC 2 cut(s) 65, 218
CviAII CATG 7 cut(s) 349, 598, 614, 733, 739, 881, 893
DdeI CTNAG 4 cut(s) 287, 429, 591, 970
DpnI GATC 3 cut(s) 159, 327, 724
DpnII GATC 3 cut(s) 157, 325, 722
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
Eam1104I CTCTTC 3 cut(s) 359, 759, 986
EarI CTCTTC 3 cut(s) 359, 759, 986
EciI GGCGGA 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 57, 832, 967
Eco81I CCTNAGG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 400, 617
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 7 cut(s) 352, 601, 617, 736, 742, 884, 896
FaqI GGGAC 3 cut(s) 470, 869, 951
FatI CATG 7 cut(s) 348, 597, 613, 732, 738, 880, 892
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 5 cut(s) 6, 9, 117, 390, 927
FokI GGATG 2 cut(s) 773, 855
Fsp4HI GCNGC 5 cut(s) 6, 9, 117, 390, 927
FspBI CTAG 6 cut(s) 104, 480, 626, 695, 744, 872
GlaI GCGC 2 cut(s) 624, 898
GluI GCNGC 5 cut(s) 6, 9, 117, 390, 927
HaeII RGCGCY 2 cut(s) 626, 900
HaeIII GGCC 2 cut(s) 66, 220
HapII CCGG 1 cut(s) 887
HhaI GCGC 2 cut(s) 625, 899
Hin1II CATG 7 cut(s) 352, 601, 617, 736, 742, 884, 896
Hin6I GCGC 2 cut(s) 623, 897
HinP1I GCGC 2 cut(s) 623, 897
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 7 cut(s) 20, 483, 534, 683, 776, 939, 1028
HpaII CCGG 1 cut(s) 887
HphI GGTGA 1 cut(s) 692
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 3 cut(s) 37, 372, 592
Hpy188III TCNNGA 5 cut(s) 155, 161, 480, 552, 812
Hpy8I GTNNAC 1 cut(s) 712
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 5 cut(s) 196, 418, 742, 763, 801
HpyCH4III ACNGT 3 cut(s) 416, 634, 709
HpyCH4IV ACGT 1 cut(s) 904
HpyCH4V TGCA 5 cut(s) 11, 197, 389, 637, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 72, 177, 578
HpyF3I CTNAG 4 cut(s) 287, 429, 591, 970
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 7 cut(s) 352, 601, 617, 736, 742, 884, 896
HspAI GCGC 2 cut(s) 623, 897
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 157, 325, 722
LguI GCTCTTC 1 cut(s) 359
Lsp1109I GCAGC 4 cut(s) 17, 103, 401, 913
LweI GCATC 4 cut(s) 103, 646, 833, 920
MaeI CTAG 6 cut(s) 104, 480, 626, 695, 744, 872
MaeII ACGT 1 cut(s) 904
MaeIII GTNAC 2 cut(s) 496, 703
MalI GATC 3 cut(s) 159, 327, 724
MboI GATC 3 cut(s) 157, 325, 722
MboII GAAGA 5 cut(s) 346, 543, 776, 973, 974
MflI RGATCY 1 cut(s) 722
MluCI AATT 6 cut(s) 263, 305, 547, 953, 1002, 1075
MlyI GAGTC 3 cut(s) 492, 933, 1037
MseI TTAA 4 cut(s) 144, 345, 783, 1062
MslI CAYNNNNRTG 3 cut(s) 675, 678, 737
MspA1I CMGCKG 2 cut(s) 8, 392
MspI CCGG 1 cut(s) 887
MspR9I CCNGG 3 cut(s) 402, 619, 887
MvaI CCWGG 2 cut(s) 402, 619
MwoI GCNNNNNNNGC 4 cut(s) 63, 72, 177, 578
NciI CCSGG 1 cut(s) 887
NdeII GATC 3 cut(s) 157, 325, 722
NlaIII CATG 7 cut(s) 352, 601, 617, 736, 742, 884, 896
NlaIV GGNNCC 2 cut(s) 67, 406
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 703
NspI RCATGY 1 cut(s) 352
OliI CACNNNNGTG 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 359
PfeI GAWTC 4 cut(s) 20, 534, 683, 776
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 5 cut(s) 7, 10, 118, 391, 928
PleI GAGTC 3 cut(s) 491, 933, 1036
PpsI GAGTC 3 cut(s) 491, 933, 1036
Psp6I CCWGG 2 cut(s) 400, 617
PspGI CCWGG 2 cut(s) 400, 617
PspN4I GGNNCC 2 cut(s) 67, 406
PspPI GGNCC 2 cut(s) 65, 218
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PstI CTGCAG 1 cut(s) 13
PsuI RGATCY 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 8
RseI CAYNNNNRTG 3 cut(s) 675, 678, 737
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 4 cut(s) 144, 345, 783, 1062
SatI GCNGC 5 cut(s) 6, 9, 117, 390, 927
Sau3AI GATC 3 cut(s) 157, 325, 722
Sau96I GGNCC 2 cut(s) 65, 218
SchI GAGTC 3 cut(s) 492, 933, 1037
ScrFI CCNGG 3 cut(s) 402, 619, 887
SfaNI GCATC 4 cut(s) 103, 646, 833, 920
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 3 cut(s) 675, 678, 737
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
SpeI ACTAGT 1 cut(s) 103
Sse9I AATT 6 cut(s) 263, 305, 547, 953, 1002, 1075
SsiI CCGC 2 cut(s) 57, 392
SspI AATATT 2 cut(s) 587, 1023
SspMI CTAG 6 cut(s) 104, 480, 626, 695, 744, 872
StyD4I CCNGG 3 cut(s) 400, 617, 885
TaaI ACNGT 3 cut(s) 416, 634, 709
TaiI ACGT 1 cut(s) 907
TaqI TCGA 4 cut(s) 89, 267, 514, 640
TasI AATT 6 cut(s) 263, 305, 547, 953, 1002, 1075
TfiI GAWTC 4 cut(s) 20, 534, 683, 776
Tru1I TTAA 4 cut(s) 144, 345, 783, 1062
Tru9I TTAA 4 cut(s) 144, 345, 783, 1062
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 5 cut(s) 5, 8, 116, 389, 926
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 4 cut(s) 458, 768, 783, 990
TspGWI ACGGA 1 cut(s) 515
TspRI CASTG 1 cut(s) 18
XapI RAATTY 3 cut(s) 263, 305, 547
XbaI TCTAGA 1 cut(s) 479
XceI RCATGY 1 cut(s) 352
XspI CTAG 6 cut(s) 104, 480, 626, 695, 744, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.