Rroxscaffold_3G00274440

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000151
Physical Location & Seq
Reverse (-)
66106428 .. 66107856
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274440.1

Sequence Viewer

Length: 1089 bp
ATGGCAGCAGAGAATACCAGTAAAGTCTTTGAAGCACATCCTATGAAAGGTGGAGATGGCCCCAATAGCTATACAAAAAACTCCATTCTGCAGAGAGCTTCTGTCGGTGCTGCCAAAGAACTTCTGAACAAGGCAATTGCAGAAAAGGTGGACATTGAAAGCTTCTTACCTTCCAAGTCTTTTCGCATTGCAGATCTGGGTTGCTCTACTGGACCAAATACTTTTATGGCAGTTGGAAACACACTTGAAGCTGTGGAGTCCAAGTATCGAAGCCAGGGGCTGAATTCTCAGATTCCTGAATTTCAAGTGTTCTTCAATGATCATACCTCAAATGACTTTAACATGCTCTTCCAGTCCCTCCCTCAGAACAGGCAATACCATGCAGCTGGTGTACCAGGTTCTTTCTACAAACAGGTGTTACCTAATGCTTCCATTAACTATGCTTACTCTTCTACTGCCATTCAATGGCTCTCTAGAGTACCAACAGCAGTAGCAGATAGTAATAGTCCTGCGTGGAACAAAGGACGCATTCATTACTCAAATGCCACAGATGAAGTAATAAGGGCTTATGAAACTCAATATAGTGATGACATGGAGAGCTTCCTGCAAGCCAGGGCACAAGAGATTGTGTATCGAGGATTAATTGTACTTACATTTCCAGGCCGCCACAGTGACACCCCTCATTCTGATGCTCCGCCAAACATGGTCTTACAGCTTTTAGGAGCCTCCCTCATGGATTTGGTTAGAAAGGGAGTAGTTAGCGAAGAGAAGGTAGATTCATTTAACATACCTGTGTATTCAATGTCTCCCAAAGAACTAGCAGCTGCTGTAGAAAGAAATGGATGTTTTAGCATAGAGATGGTGGCAGACTTGCTTGTTCCCCCGGTAGATGACACTAGCTCAATGCCACAACTAATTGCCTCTCACATTAGAGCTGGCATGGAGGGGATATTGAAGCAGCAATTTGGAGAGGAACTTTTAGATGAGCTCTTCGATTTGTATCAGGAAAAATGTGAAGAGTATATCTCCACCACAAACACAGGGAAGCCAGTTAACTTCCTTGTGGTGCTTAGACGCAAGGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

362

Amino Acids

40.08

Weight (kDa)

5.14

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 359 4.5e-102 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 465
AciI CCGC 2 cut(s) 664, 695
AcsI RAATTY 2 cut(s) 283, 299
AfaI GTAC 3 cut(s) 393, 480, 648
AfiI CCNNNNNNNGG 2 cut(s) 47, 465
AgsI TTSAA 8 cut(s) 32, 158, 248, 305, 316, 464, 801, 955
AjnI CCWGG 4 cut(s) 273, 394, 611, 658
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 1 cut(s) 810
AlwNI CAGNNNCTG 2 cut(s) 280, 827
AoxI GGCC 2 cut(s) 58, 661
ApeKI GCWGC 6 cut(s) 5, 110, 383, 821, 824, 958
ApoI RAATTY 2 cut(s) 283, 299
AseI ATTAAT 1 cut(s) 641
AspS9I GGNCC 2 cut(s) 59, 212
AsuC2I CCSGG 1 cut(s) 884
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 990
Bbv12I GWGCWC 1 cut(s) 990
BbvI GCAGC 6 cut(s) 17, 97, 395, 811, 833, 970
BccI CCATC 2 cut(s) 50, 853
BciT130I CCWGG 4 cut(s) 275, 396, 613, 660
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 884
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 3 cut(s) 474, 818, 897
BfmI CTRYAG 2 cut(s) 89, 828
BglII AGATCT 1 cut(s) 193
BisI GCNGC 7 cut(s) 6, 111, 384, 664, 822, 825, 959
BlsI GCNGC 7 cut(s) 7, 112, 385, 665, 823, 826, 960
Bme1390I CCNGG 5 cut(s) 275, 396, 613, 660, 884
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 59, 212
BmiI GGNNCC 2 cut(s) 61, 724
BmrFI CCNGG 5 cut(s) 275, 396, 613, 660, 884
BmsI GCATC 1 cut(s) 679
BplI GAGNNNNNCTC 4 cut(s) 714, 746, 1010, 1042
BpuMI CCSGG 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 3 cut(s) 274, 612, 882
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 465
Bse1I ACTGG 4 cut(s) 18, 214, 352, 1049
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 4 cut(s) 275, 396, 613, 660
BseDI CCNNGG 3 cut(s) 274, 612, 882
BseGI GGATG 2 cut(s) 37, 848
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 2 cut(s) 47, 465
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 302, 377
BseNI ACTGG 4 cut(s) 18, 214, 352, 1049
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 6 cut(s) 17, 97, 395, 811, 833, 970
BshFI GGCC 2 cut(s) 60, 663
BsiHKAI GWGCWC 1 cut(s) 990
BsiSI CCGG 1 cut(s) 884
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 2 cut(s) 47, 465
BsmAI GTCTC 1 cut(s) 810
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 2 cut(s) 60, 663
Bsp1286I GDGCHC 2 cut(s) 619, 990
Bsp143I GATC 2 cut(s) 193, 319
BspACI CCGC 2 cut(s) 664, 695
BspANI GGCC 2 cut(s) 60, 663
BspCNI CTCAG 2 cut(s) 301, 376
BspLI GGNNCC 2 cut(s) 61, 724
BspMAI CTGCAG 1 cut(s) 93
BspQI GCTCTTC 2 cut(s) 353, 995
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 4 cut(s) 18, 214, 352, 1049
BssECI CCNNGG 3 cut(s) 274, 612, 882
BssMI GATC 2 cut(s) 193, 319
Bst2UI CCWGG 4 cut(s) 275, 396, 613, 660
Bst4CI ACNGT 1 cut(s) 671
Bst6I CTCTTC 5 cut(s) 353, 454, 759, 995, 1011
BstC8I GCNNGC 2 cut(s) 609, 937
BstDEI CTNAG 3 cut(s) 288, 363, 1070
BstENI CCTNNNNNAGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 37, 848
BstKTI GATC 2 cut(s) 196, 322
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 2 cut(s) 193, 319
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 4 cut(s) 275, 396, 613, 660
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 5 cut(s) 273, 394, 611, 658, 882
BstSFI CTRYAG 2 cut(s) 89, 828
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 6 cut(s) 17, 97, 395, 811, 833, 970
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 386
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 2 cut(s) 60, 663
BtsCI GGATG 2 cut(s) 37, 848
BtsIMutI CAGTG 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 609, 937
CaiI CAGNNNCTG 2 cut(s) 280, 827
Cfr13I GGNCC 2 cut(s) 59, 212
CseI GACGC 2 cut(s) 534, 1083
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 3 cut(s) 392, 479, 647
CviAII CATG 6 cut(s) 343, 380, 592, 703, 733, 940
CviQI GTAC 3 cut(s) 392, 479, 647
DdeI CTNAG 3 cut(s) 288, 363, 1070
DpnI GATC 2 cut(s) 195, 321
DpnII GATC 2 cut(s) 193, 319
Eam1104I CTCTTC 5 cut(s) 353, 454, 759, 995, 1011
EarI CTCTTC 5 cut(s) 353, 454, 759, 995, 1011
EciI GGCGGA 1 cut(s) 684
Ecl136II GAGCTC 1 cut(s) 988
Eco24I GRGCYC 1 cut(s) 990
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 988
EcoICRI GAGCTC 1 cut(s) 988
EcoNI CCTNNNNNAGG 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 4 cut(s) 273, 394, 611, 658
EcoT38I GRGCYC 1 cut(s) 990
FaeI CATG 6 cut(s) 346, 383, 595, 706, 736, 943
FaqI GGGAC 1 cut(s) 340
FatI CATG 6 cut(s) 342, 379, 591, 702, 732, 939
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 7 cut(s) 6, 111, 384, 664, 822, 825, 959
FokI GGATG 2 cut(s) 24, 855
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 7 cut(s) 6, 111, 384, 664, 822, 825, 959
FspBI CTAG 3 cut(s) 474, 818, 897
GluI GCNGC 7 cut(s) 6, 111, 384, 664, 822, 825, 959
HaeIII GGCC 2 cut(s) 60, 663
HapII CCGG 1 cut(s) 884
HgaI GACGC 2 cut(s) 534, 1083
Hin1II CATG 6 cut(s) 346, 383, 595, 706, 736, 943
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 1 cut(s) 160
HinfI GANTC 3 cut(s) 257, 292, 776
HpaI GTTAAC 1 cut(s) 1054
HpaII CCGG 1 cut(s) 884
Hpy166II GTNNAC 3 cut(s) 151, 392, 1054
Hpy188I TCNGA 4 cut(s) 126, 291, 366, 688
Hpy188III TCNNGA 3 cut(s) 296, 474, 1004
Hpy8I GTNNAC 3 cut(s) 151, 392, 1054
HpyAV CCTTC 2 cut(s) 180, 763
HpyCH4III ACNGT 1 cut(s) 671
HpyCH4V TGCA 5 cut(s) 91, 140, 191, 383, 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 3 cut(s) 288, 363, 1070
Hsp92II CATG 6 cut(s) 346, 383, 595, 706, 736, 943
Ksp22I TGATCA 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 1054
Kzo9I GATC 2 cut(s) 193, 319
LguI GCTCTTC 2 cut(s) 353, 995
LmnI GCTCC 2 cut(s) 697, 722
Lsp1109I GCAGC 6 cut(s) 17, 97, 395, 811, 833, 970
LweI GCATC 1 cut(s) 679
MabI ACCWGGT 1 cut(s) 394
MaeI CTAG 3 cut(s) 474, 818, 897
MaeIII GTNAC 2 cut(s) 417, 671
MalI GATC 2 cut(s) 195, 321
MboI GATC 2 cut(s) 193, 319
MboII GAAGA 6 cut(s) 304, 340, 441, 776, 982, 1028
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 619, 990
MluCI AATT 6 cut(s) 135, 283, 299, 642, 915, 962
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 214
MseI TTAA 5 cut(s) 339, 435, 641, 783, 1053
MslI CAYNNNNRTG 3 cut(s) 687, 791, 857
MspA1I CMGCKG 2 cut(s) 386, 824
MspI CCGG 1 cut(s) 884
MspR9I CCNGG 5 cut(s) 275, 396, 613, 660, 884
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 4 cut(s) 275, 396, 613, 660
MwoI GCNNNNNNNGC 1 cut(s) 66
NciI CCSGG 1 cut(s) 884
NdeII GATC 2 cut(s) 193, 319
NlaIII CATG 6 cut(s) 346, 383, 595, 706, 736, 943
NlaIV GGNNCC 2 cut(s) 61, 724
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 346
PciSI GCTCTTC 2 cut(s) 353, 995
PctI GAATGC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 292, 776
PflMI CCANNNNNTGG 1 cut(s) 465
PkrI GCNGC 7 cut(s) 7, 112, 385, 665, 823, 826, 960
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PshBI ATTAAT 1 cut(s) 641
Psp124BI GAGCTC 1 cut(s) 990
Psp6I CCWGG 4 cut(s) 273, 394, 611, 658
PspGI CCWGG 4 cut(s) 273, 394, 611, 658
PspN4I GGNNCC 2 cut(s) 61, 724
PspPI GGNCC 2 cut(s) 59, 212
PsrI GAACNNNNNNTAC 2 cut(s) 359, 391
PstI CTGCAG 1 cut(s) 93
PstNI CAGNNNCTG 2 cut(s) 280, 827
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 386, 824
RsaI GTAC 3 cut(s) 393, 480, 648
RsaNI GTAC 3 cut(s) 392, 479, 647
RseI CAYNNNNRTG 3 cut(s) 687, 791, 857
SacI GAGCTC 1 cut(s) 990
SapI GCTCTTC 2 cut(s) 353, 995
SaqAI TTAA 5 cut(s) 339, 435, 641, 783, 1053
SatI GCNGC 7 cut(s) 6, 111, 384, 664, 822, 825, 959
Sau3AI GATC 2 cut(s) 193, 319
Sau96I GGNCC 2 cut(s) 59, 212
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 5 cut(s) 275, 396, 613, 660, 884
SduI GDGCHC 2 cut(s) 619, 990
SexAI ACCWGGT 1 cut(s) 394
SfaNI GCATC 1 cut(s) 679
SfcI CTRYAG 2 cut(s) 89, 828
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 3 cut(s) 687, 791, 857
Sse9I AATT 6 cut(s) 135, 283, 299, 642, 915, 962
SsiI CCGC 2 cut(s) 664, 695
SspMI CTAG 3 cut(s) 474, 818, 897
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 5 cut(s) 273, 394, 611, 658, 882
TaaI ACNGT 1 cut(s) 671
TaqI TCGA 3 cut(s) 268, 634, 993
TasI AATT 6 cut(s) 135, 283, 299, 642, 915, 962
TatI WGTACW 1 cut(s) 646
TauI GCSGC 1 cut(s) 666
TfiI GAWTC 2 cut(s) 292, 776
Tru1I TTAA 5 cut(s) 339, 435, 641, 783, 1053
Tru9I TTAA 5 cut(s) 339, 435, 641, 783, 1053
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 6 cut(s) 5, 110, 383, 821, 824, 958
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 5 cut(s) 59, 521, 567, 585, 768
TspRI CASTG 1 cut(s) 676
Van91I CCANNNNNTGG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 212
VspI ATTAAT 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 45
XapI RAATTY 2 cut(s) 283, 299
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 1 cut(s) 346
XspI CTAG 3 cut(s) 474, 818, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.