RchiOBHm_Chr4g0400971

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000151
Physical Location & Seq
Reverse (-)
18950893 .. 18952122
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37296

Sequence Viewer

Length: 564 bp
ATGGCGTCAGAGGAAATCCGAAATGTTTCTGAAGGATATCCAATGAAAGGTGGAGATGGCCCCGATAGCTATGCCAAGAACTCTACTTACCAGAGAAAAGCAATGGAGTCTGTTAAAGAACTTGTTACCAAAGGAATTGCAGAACAGCTTGACATAGACCTTTTGTTACCTTCCAACTCCTTTCACATTGCGGATCTTGGTTGCTCTGTTGGTCCTAATACATTTTCTTCAGTTGAAAACATACTTGAAGCTGTACAGCTCAAGTTTCAAAGCCAAGGACTCATGAATCACCAAATCCCTGAATTTCAAGTTTTCTTCAATGATCATACCCCAAATGATTTTAACTTGCTCTTCAAATCCCTCCCTTCCAACAGGCAATACTATGCTGCGGGAGTTCCAGGTTCTTTTTACGGTCGCCTATTCCCTAGTGCTTCCATTCACTTGTTTCACTCTTCTTTTGCTCTTCAATGGCTTTCTAAAGTACCAAAAGATGTAGAGGACAAAAACAGTCCGGCTTGCAGGGACTTGCCTTCTATTTTTCATGCTAAGAATGTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

187

Amino Acids

20.85

Weight (kDa)

6.05

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 173 4.6e-49 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 191, 389
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 2 cut(s) 51, 213
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 255, 483
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 7 cut(s) 236, 248, 269, 308, 319, 355, 467
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 4 cut(s) 69, 148, 251, 259
AluI AGCT 4 cut(s) 69, 148, 251, 259
AlwI GGATC 1 cut(s) 201
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 25
AspS9I GGNCC 2 cut(s) 59, 212
AsuHPI GGTGA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 1 cut(s) 399
BclI TGATCA 1 cut(s) 322
BfaI CTAG 1 cut(s) 426
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 59, 212
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse3DI GCAATG 2 cut(s) 108, 186
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 2 cut(s) 108, 186
BseXI GCAGC 1 cut(s) 373
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 415
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 253
Bsp143I GATC 2 cut(s) 193, 322
BspACI CCGC 2 cut(s) 191, 389
BspANI GGCC 1 cut(s) 60
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 1 cut(s) 201
BspQI GCTCTTC 2 cut(s) 356, 468
BsrDI GCAATG 2 cut(s) 108, 186
BsrGI TGTACA 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 2 cut(s) 193, 322
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 413, 509
Bst6I CTCTTC 3 cut(s) 356, 457, 468
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 1 cut(s) 546
BstKTI GATC 2 cut(s) 196, 325
BstMBI GATC 2 cut(s) 193, 322
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 397
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 517
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 59, 212
Csp6I GTAC 2 cut(s) 254, 482
CviAII CATG 2 cut(s) 283, 542
CviJI RGCY 8 cut(s) 60, 69, 148, 251, 259, 273, 472, 515
CviKI_1 RGCY 8 cut(s) 60, 69, 148, 251, 259, 273, 472, 515
CviQI GTAC 2 cut(s) 254, 482
DdeI CTNAG 1 cut(s) 546
DpnI GATC 2 cut(s) 195, 324
DpnII GATC 2 cut(s) 193, 322
Eam1104I CTCTTC 3 cut(s) 356, 457, 468
EarI CTCTTC 3 cut(s) 356, 457, 468
Eco130I CCWWGG 1 cut(s) 274
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 51, 213
EcoRII CCWGG 1 cut(s) 397
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 286, 545
FaiI YATR 7 cut(s) 72, 155, 242, 284, 327, 384, 543
FaqI GGGAC 1 cut(s) 536
FatI CATG 2 cut(s) 282, 541
FauI CCCGC 1 cut(s) 382
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 387
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 512
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 286, 545
HinfI GANTC 3 cut(s) 107, 279, 286
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 281
Hpy188I TCNGA 3 cut(s) 10, 20, 31
Hpy188III TCNNGA 2 cut(s) 283, 556
HpyAV CCTTC 4 cut(s) 26, 180, 375, 540
HpyCH4III ACNGT 2 cut(s) 413, 509
HpyCH4V TGCA 2 cut(s) 140, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 1 cut(s) 546
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 286, 545
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 193, 322
LguI GCTCTTC 2 cut(s) 356, 468
LpnPI CCDG 7 cut(s) 104, 312, 358, 384, 411, 505, 525
Lsp1109I GCAGC 1 cut(s) 373
MaeI CTAG 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 124, 165
MalI GATC 2 cut(s) 195, 324
MboI GATC 2 cut(s) 193, 322
MboII GAAGA 5 cut(s) 219, 307, 343, 444, 455
MflI RGATCY 1 cut(s) 193
MluCI AATT 2 cut(s) 135, 302
MlyI GAGTC 2 cut(s) 116, 273
MmeI TCCRAC 2 cut(s) 198, 393
MnlI CCTC 3 cut(s) 4, 371, 490
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 3 cut(s) 114, 342, 562
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 66
NdeII GATC 2 cut(s) 193, 322
NlaIII CATG 2 cut(s) 286, 545
NlaIV GGNNCC 1 cut(s) 61
PagI TCATGA 1 cut(s) 282
PciSI GCTCTTC 2 cut(s) 356, 468
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 2 cut(s) 115, 273
PpsI GAGTC 2 cut(s) 115, 273
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 59, 212
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 2 cut(s) 255, 483
RsaNI GTAC 2 cut(s) 254, 482
SapI GCTCTTC 2 cut(s) 356, 468
SaqAI TTAA 3 cut(s) 114, 342, 562
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 2 cut(s) 193, 322
Sau96I GGNCC 2 cut(s) 59, 212
SchI GAGTC 2 cut(s) 116, 273
ScrFI CCNGG 1 cut(s) 399
SetI ASST 8 cut(s) 52, 71, 150, 162, 172, 253, 261, 403
SinI GGWCC 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 135, 302
SsiI CCGC 2 cut(s) 191, 389
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 2 cut(s) 413, 509
TasI AATT 2 cut(s) 135, 302
TatI WGTACW 1 cut(s) 253
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 3 cut(s) 114, 342, 562
Tru9I TTAA 3 cut(s) 114, 342, 562
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 3 cut(s) 59, 299, 530
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.