Rw3G024220

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000151
Physical Location & Seq
Reverse (-)
31014368 .. 31015922
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024220.1

Sequence Viewer

Length: 966 bp
ATGAAAGGTGGAGATGGCCCCAGCAACTATGCCAAGAACTCCATCTACCAGAAAGGAGTAATTGATGCTGCCAAAGAACTTCTAACTAAGGCGATTGCAGAAAAGCTTGACATGGAAACATTTTCATCCGCCAACTCCTTTCACATTGCAGATTTGGGTTGCTCAGTTGGGCCCAATACGTTTTTGGCGGTTGAAAATATACTTGAAGCTGTGCTATTCAAGTATCAAAGCCGAGGGCCGAATTGTCAAATCCCTGAATTTCAAGTCTTCTTTAATGATCATACCTCAAATGACTTCAACATGCTCTTCAATTCCCTCCCTCAGAATAGGCAATACTATGTTGCGGTTGTGCCTGTACCAAAAGATGTAACAGACAGCAACTCCCCTGCTTGGAATAAAGGACGAATGCATTACTTAGACTCCACAGATGAAGTAGTGAGGGCTTACGAAGCCCAATCTGCCGAGGACATGGAGTGCTTTCTGCATGCCAGGGCACAAGAGACAGTACATGGAGGATTGATGGTACTTGCTACTGCTGGTTACCCAGCTGATACACCTCCTTCTCATTCTCTGGCAAATGTCACCTATCAAATTTTAGGATCTTGCCTCATTGACATGTCTAGGAAGGGAGTAGTCAGCGAGGAGGAAATAGATTCATTTAATGTACCTATATACTATGTGTGTCCCCGAGAACTGGAAGCTGCTGTAGAACGAAATGGATGTTTTAGCATAGAGATAATGGAACACTTACCTACTGTGATGGAACCCGACACTTCAAAAAATAGCAAACTCTTTGCATCTCATATGAGAGCTGCCATGGAGGGACTCTTCAAGCAGCATTTCGGAGAAGAAATCTTAGACGAGCTCTTCGACTTGTTTCACAAGAAAGTTGAAGAGCAGCACTCCGCATTTGAGTCAGGGAAGGCAGGGGACATCTTTATTGTGCTTAAACGCAAGGCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

321

Amino Acids

35.81

Weight (kDa)

5.04

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 36 - 113 3.3e-25 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 119 - 318 2.3e-52 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 129, 188, 344, 906
AclWI GGATC 1 cut(s) 607
AcsI RAATTY 2 cut(s) 257, 591
AfaI GTAC 4 cut(s) 357, 507, 525, 666
AfiI CCNNNNNNNGG 2 cut(s) 390, 694
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 9 cut(s) 194, 206, 220, 263, 298, 310, 777, 832, 893
AjnI CCWGG 1 cut(s) 488
AluBI AGCT 6 cut(s) 106, 209, 548, 701, 812, 865
AluI AGCT 6 cut(s) 106, 209, 548, 701, 812, 865
Alw21I GWGCWC 1 cut(s) 867
Alw26I GTCTC 1 cut(s) 494
AlwI GGATC 1 cut(s) 607
Ama87I CYCGRG 1 cut(s) 687
AoxI GGCC 3 cut(s) 16, 170, 236
ApaI GGGCCC 1 cut(s) 174
ApeKI GCWGC 5 cut(s) 68, 701, 812, 835, 898
ApoI RAATTY 2 cut(s) 257, 591
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AspS9I GGNCC 4 cut(s) 17, 170, 171, 236
AsuHPI GGTGA 1 cut(s) 574
AvaI CYCGRG 1 cut(s) 687
BaeGI GKGCMC 2 cut(s) 174, 496
BanII GRGCYC 2 cut(s) 174, 867
BbsI GAAGAC 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 867
BbvI GCAGC 5 cut(s) 55, 688, 799, 847, 910
BccI CCATC 4 cut(s) 8, 50, 514, 754
BciT130I CCWGG 1 cut(s) 490
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 494
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 5 cut(s) 69, 702, 813, 836, 899
BlsI GCNGC 5 cut(s) 70, 703, 814, 837, 900
Bme1390I CCNGG 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 687
BmgT120I GGNCC 4 cut(s) 17, 170, 171, 236
BmiI GGNNCC 3 cut(s) 19, 172, 765
BmrFI CCNGG 1 cut(s) 490
BmsI GCATC 2 cut(s) 55, 806
BpiI GAAGAC 1 cut(s) 259
BplI GAGNNNNNCTC 2 cut(s) 887, 919
BsaJI CCNNGG 4 cut(s) 232, 462, 489, 816
BsaXI ACNNNNNCTCC 4 cut(s) 365, 395, 404, 434
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 694
Bse1I ACTGG 1 cut(s) 699
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 4 cut(s) 232, 462, 489, 816
BseGI GGATG 2 cut(s) 125, 725
BseLI CCNNNNNNNGG 2 cut(s) 390, 694
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 177, 335
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 2 cut(s) 174, 496
BseXI GCAGC 5 cut(s) 55, 688, 799, 847, 910
BseYI CCCAGC 2 cut(s) 20, 544
BshFI GGCC 3 cut(s) 18, 172, 238
BsiHKAI GWGCWC 1 cut(s) 867
BsiHKCI CYCGRG 1 cut(s) 687
BslFI GGGAC 3 cut(s) 669, 837, 944
BslI CCNNNNNNNGG 2 cut(s) 390, 694
BsmAI GTCTC 1 cut(s) 494
BsmFI GGGAC 3 cut(s) 669, 837, 944
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 3 cut(s) 18, 172, 238
BsoBI CYCGRG 1 cut(s) 687
Bsp120I GGGCCC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 174, 496, 867
Bsp143I GATC 2 cut(s) 277, 599
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 4 cut(s) 129, 188, 344, 906
BspANI GGCC 3 cut(s) 18, 172, 238
BspCNI CTCAG 2 cut(s) 176, 334
BspLI GGNNCC 3 cut(s) 19, 172, 765
BspPI GGATC 1 cut(s) 607
BspQI GCTCTTC 3 cut(s) 311, 872, 888
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 1 cut(s) 699
BssECI CCNNGG 4 cut(s) 232, 462, 489, 816
BssMI GATC 2 cut(s) 277, 599
BssT1I CCWWGG 1 cut(s) 816
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 505, 757
Bst6I CTCTTC 4 cut(s) 311, 833, 872, 888
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 5 cut(s) 87, 163, 321, 415, 856
BstDSI CCRYGG 1 cut(s) 816
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 2 cut(s) 125, 725
BstKTI GATC 2 cut(s) 280, 602
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 2 cut(s) 277, 599
BstMWI GCNNNNNNNGC 2 cut(s) 449, 458
BstNI CCWGG 1 cut(s) 490
BstNSI RCATGY 3 cut(s) 304, 488, 619
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 488
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 2 cut(s) 174, 496
BstV1I GCAGC 5 cut(s) 55, 688, 799, 847, 910
BstV2I GAAGAC 1 cut(s) 259
BstX2I RGATCY 1 cut(s) 599
BstYI RGATCY 1 cut(s) 599
BsuRI GGCC 3 cut(s) 18, 172, 238
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 2 cut(s) 125, 725
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 4 cut(s) 17, 170, 171, 236
Csp6I GTAC 4 cut(s) 356, 506, 524, 665
CviAII CATG 7 cut(s) 112, 301, 469, 485, 509, 616, 817
CviQI GTAC 4 cut(s) 356, 506, 524, 665
DdeI CTNAG 5 cut(s) 87, 163, 321, 415, 856
DpnI GATC 2 cut(s) 279, 601
DpnII GATC 2 cut(s) 277, 599
Eam1104I CTCTTC 4 cut(s) 311, 833, 872, 888
EarI CTCTTC 4 cut(s) 311, 833, 872, 888
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 1 cut(s) 816
Eco24I GRGCYC 2 cut(s) 174, 867
Eco53kI GAGCTC 1 cut(s) 865
Eco88I CYCGRG 1 cut(s) 687
Eco91I GGTNACC 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 865
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 816
EcoT22I ATGCAT 1 cut(s) 411
EcoT38I GRGCYC 2 cut(s) 174, 867
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 7 cut(s) 115, 304, 472, 488, 512, 619, 820
FaqI GGGAC 3 cut(s) 669, 837, 944
FatI CATG 7 cut(s) 111, 300, 468, 484, 508, 615, 816
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 5 cut(s) 69, 702, 813, 836, 899
FokI GGATG 2 cut(s) 112, 732
FriOI GRGCYC 2 cut(s) 174, 867
Fsp4HI GCNGC 5 cut(s) 69, 702, 813, 836, 899
FspBI CTAG 1 cut(s) 621
GluI GCNGC 5 cut(s) 69, 702, 813, 836, 899
GsaI CCCAGC 2 cut(s) 24, 548
HaeIII GGCC 3 cut(s) 18, 172, 238
Hin1II CATG 7 cut(s) 115, 304, 472, 488, 512, 619, 820
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 4 cut(s) 419, 653, 825, 914
HphI GGTGA 1 cut(s) 574
Hpy188I TCNGA 2 cut(s) 324, 845
HpyAV CCTTC 3 cut(s) 570, 619, 916
HpyCH4III ACNGT 2 cut(s) 505, 757
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 98, 149, 409, 484, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 458
HpyF3I CTNAG 5 cut(s) 87, 163, 321, 415, 856
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 7 cut(s) 115, 304, 472, 488, 512, 619, 820
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 2 cut(s) 277, 599
LguI GCTCTTC 3 cut(s) 311, 872, 888
Lsp1109I GCAGC 5 cut(s) 55, 688, 799, 847, 910
LweI GCATC 2 cut(s) 55, 806
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 367, 539, 580
MalI GATC 2 cut(s) 279, 601
MboI GATC 2 cut(s) 277, 599
MboII GAAGA 6 cut(s) 259, 298, 820, 859, 860, 905
MflI RGATCY 1 cut(s) 599
MhlI GDGCHC 3 cut(s) 174, 496, 867
MluCI AATT 6 cut(s) 60, 241, 257, 310, 591, 961
MlyI GAGTC 3 cut(s) 413, 819, 923
Mph1103I ATGCAT 1 cut(s) 411
MseI TTAA 3 cut(s) 273, 660, 948
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 490
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 449, 458
NcoI CCATGG 1 cut(s) 816
NdeI CATATG 1 cut(s) 804
NdeII GATC 2 cut(s) 277, 599
NlaIII CATG 7 cut(s) 115, 304, 472, 488, 512, 619, 820
NlaIV GGNNCC 3 cut(s) 19, 172, 765
NmeAIII GCCGAG 2 cut(s) 257, 487
NmuCI GTSAC 1 cut(s) 580
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 3 cut(s) 304, 488, 619
PaeI GCATGC 1 cut(s) 488
PciI ACATGT 1 cut(s) 615
PciSI GCTCTTC 3 cut(s) 311, 872, 888
PcsI WCGNNNNNNNCGW 1 cut(s) 867
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 653
PkrI GCNGC 5 cut(s) 70, 703, 814, 837, 900
PleI GAGTC 3 cut(s) 413, 819, 922
PpsI GAGTC 3 cut(s) 413, 819, 922
PscI ACATGT 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 488
PspEI GGTNACC 1 cut(s) 539
PspFI CCCAGC 2 cut(s) 20, 544
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 3 cut(s) 19, 172, 765
PspOMI GGGCCC 1 cut(s) 170
PspPI GGNCC 4 cut(s) 17, 170, 171, 236
PsrI GAACNNNNNNTAC 2 cut(s) 29, 61
PsuI RGATCY 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 4 cut(s) 357, 507, 525, 666
RsaNI GTAC 4 cut(s) 356, 506, 524, 665
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 867
SapI GCTCTTC 3 cut(s) 311, 872, 888
SaqAI TTAA 3 cut(s) 273, 660, 948
SatI GCNGC 5 cut(s) 69, 702, 813, 836, 899
Sau3AI GATC 2 cut(s) 277, 599
Sau96I GGNCC 4 cut(s) 17, 170, 171, 236
SchI GAGTC 3 cut(s) 413, 819, 923
ScrFI CCNGG 1 cut(s) 490
SduI GDGCHC 3 cut(s) 174, 496, 867
SfaNI GCATC 2 cut(s) 55, 806
SfcI CTRYAG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 614
SphI GCATGC 1 cut(s) 488
Sse9I AATT 6 cut(s) 60, 241, 257, 310, 591, 961
SsiI CCGC 4 cut(s) 129, 188, 344, 906
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 1 cut(s) 867
StyD4I CCNGG 1 cut(s) 488
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 2 cut(s) 505, 757
TaiI ACGT 1 cut(s) 182
TaqI TCGA 1 cut(s) 870
TasI AATT 6 cut(s) 60, 241, 257, 310, 591, 961
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 1 cut(s) 653
Tru1I TTAA 3 cut(s) 273, 660, 948
Tru9I TTAA 3 cut(s) 273, 660, 948
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 5 cut(s) 68, 701, 812, 835, 898
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 4 cut(s) 17, 114, 444, 645
XapI RAATTY 2 cut(s) 257, 591
XceI RCATGY 3 cut(s) 304, 488, 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XspI CTAG 1 cut(s) 621
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.