MD14G1232400.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000151
Physical Location & Seq
Reverse (-)
31244221 .. 31246319
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1232400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGCTGCAGCTGAGGCGGAAACCAGTATGTCCGCCGAAGCATATCCGATGAAAGGCGGAGACGGCCCCAACAGCTATGCTAAAAACTCTGCTTACCAGAGAGGAGTAGTGGATGCTGCCCAACAACTTATAAGCAGGGCAATTGCAGAAAATCTTGACGTCGAGATGTTACCTTCTTCGAACACCTTTTGCATTGCAGATTTGGGTTGTTCTATCGGGCCAAATACGTTTATTGCGGTTCAAAACATACTTGAAGCTGTAGAAATCAAGTATCAAGGCCAAGGCTTGAATTCGCATATCCCGGAATTTCATGTTTTCTTTAATGATCACACTCTAAACGATTTTAACACGCTCTTCCAATCCCTCCCTACGAGCAGGCGATACTATGCTGCAGGCGTACCCGGTTCTTTCTACGTTCGTGTATTTCCTAATGCTTCCATTCACTTTTTTCACTCTTCTTGTGCCCTTCAATGGCTTTCTAGAGTGCCGAAAGAGGTAGTGGACAGAAACAGCCCTGCGTGGAACAAAGGACGAATCGTTTATTCAAATTCCACTGATGAAGTCATAAGGGCTTATGAAGCTCAACATGCTGAGGACATGGAATGTTTCCTGCATGCCAGGGCACAAGAGATTGCGAATGGAGGATTGATGGTGCTCATCATTCCAGGACGCCCGAATGGTACCCCTCATTCGCTTTCTTCGGTAAACCAGGCTCATCAACTTCTCGGGTCTTGCCTCATGGACCTGGCTATGAAGGGAGTAGTTCAGGAAGAAAAAGTAGATTCTTTTAATATACCTCTGTATTACATGTCTCCCCAAGAACTCGAAGCTGCTGTGGAGAAAAATGGTTGTTTTAGCGCACAGAGAATTGAAAACTTGCCTCGTGTCTCGGCACTTGACATTGTTACTAAAAATAGCCAGGTATTTGCATCTCATGTCAGAGCTGCCACGGAGGGACTCGTGAAGCAACAATTTGGAGACAATATATTAGACGAGCTCTATGACTTGTATCGCAAGAGACTTGAAGAGCAACCATCGATCTTTGAGTCTGGGAAGGCGATTAACTTTCTTGTTGTGCTTAAACGCCTGGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

366

Amino Acids

40.47

Weight (kDa)

5.42

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 52 - 362 5e-105 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AatII GACGTC 1 cut(s) 162
Acc65I GGTACC 1 cut(s) 680
AccB1I GGYRCC 1 cut(s) 680
AciI CCGC 4 cut(s) 17, 33, 57, 236
AcsI RAATTY 4 cut(s) 289, 305, 547, 1092
AcyI GRCGYC 2 cut(s) 159, 670
AfaI GTAC 2 cut(s) 399, 682
AfiI CCNNNNNNNGG 2 cut(s) 53, 471
AflIII ACRYGT 1 cut(s) 807
AgsI TTSAA 7 cut(s) 242, 254, 289, 470, 546, 872, 1025
AjnI CCWGG 6 cut(s) 617, 664, 708, 744, 918, 1086
AluBI AGCT 7 cut(s) 11, 75, 257, 581, 830, 944, 997
AluI AGCT 7 cut(s) 11, 75, 257, 581, 830, 944, 997
Alw21I GWGCWC 2 cut(s) 657, 999
Alw26I GTCTC 5 cut(s) 54, 816, 892, 972, 1012
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 3 cut(s) 64, 218, 277
ApeKI GCWGC 6 cut(s) 5, 8, 116, 389, 830, 944
ApoI RAATTY 4 cut(s) 289, 305, 547, 1092
Asp718I GGTACC 1 cut(s) 680
AspLEI GCGC 1 cut(s) 860
AspS9I GGNCC 3 cut(s) 65, 218, 742
AsuC2I CCSGG 2 cut(s) 302, 402
AsuII TTCGAA 1 cut(s) 179
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 1 cut(s) 742
BaeGI GKGCMC 2 cut(s) 466, 625
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 2 cut(s) 882, 959
Bbv12I GWGCWC 2 cut(s) 657, 999
BbvCI CCTCAGC 2 cut(s) 12, 591
BbvI GCAGC 5 cut(s) 20, 103, 376, 817, 931
BccI CCATC 2 cut(s) 643, 1042
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 6 cut(s) 619, 666, 710, 746, 920, 1088
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 302, 402
BcoDI GTCTC 5 cut(s) 54, 816, 892, 972, 1012
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 3 cut(s) 6, 258, 390
BisI GCNGC 6 cut(s) 6, 9, 117, 390, 831, 945
BlsI GCNGC 6 cut(s) 7, 10, 118, 391, 832, 946
Bme1390I CCNGG 8 cut(s) 302, 402, 619, 666, 710, 746, 920, 1088
Bme18I GGWCC 1 cut(s) 742
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 3 cut(s) 65, 218, 742
BmiI GGNNCC 2 cut(s) 67, 682
BmrFI CCNGG 8 cut(s) 302, 402, 619, 666, 710, 746, 920, 1088
BmsI GCATC 2 cut(s) 103, 938
Bpu10I CCTNAGC 2 cut(s) 12, 591
Bpu14I TTCGAA 1 cut(s) 179
BpuMI CCSGG 2 cut(s) 302, 402
Bsa29I ATCGAT 1 cut(s) 1037
BsaHI GRCGYC 2 cut(s) 159, 670
BsaJI CCNNGG 3 cut(s) 280, 618, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 471
Bse1I ACTGG 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 6 cut(s) 619, 666, 710, 746, 920, 1088
BseCI ATCGAT 1 cut(s) 1037
BseDI CCNNGG 3 cut(s) 280, 618, 948
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 53, 471
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 117
BseSI GKGCMC 2 cut(s) 466, 625
BseXI GCAGC 5 cut(s) 20, 103, 376, 817, 931
BshFI GGCC 3 cut(s) 66, 220, 279
BshNI GGYRCC 1 cut(s) 680
BshVI ATCGAT 1 cut(s) 1037
BsiHKAI GWGCWC 2 cut(s) 657, 999
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 2 cut(s) 302, 402
BslFI GGGAC 1 cut(s) 969
BslI CCNNNNNNNGG 2 cut(s) 53, 471
BsmAI GTCTC 5 cut(s) 54, 816, 892, 972, 1012
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 969
BsnI GGCC 3 cut(s) 66, 220, 279
BsoBI CYCGRG 1 cut(s) 725
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 4 cut(s) 466, 625, 657, 999
Bsp143I GATC 2 cut(s) 325, 1038
BspACI CCGC 4 cut(s) 17, 33, 57, 236
BspANI GGCC 3 cut(s) 66, 220, 279
BspCNI CTCAG 2 cut(s) 4, 583
BspDI ATCGAT 1 cut(s) 1037
BspLI GGNNCC 2 cut(s) 67, 682
BspMAI CTGCAG 2 cut(s) 10, 394
BspQI GCTCTTC 2 cut(s) 359, 1020
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 3 cut(s) 280, 618, 948
BssMI GATC 2 cut(s) 325, 1038
BssNI GRCGYC 2 cut(s) 159, 670
BssSI CACGAG 2 cut(s) 882, 959
BssT1I CCWWGG 1 cut(s) 280
Bst2BI CACGAG 2 cut(s) 882, 959
Bst2UI CCWGG 6 cut(s) 619, 666, 710, 746, 920, 1088
Bst6I CTCTTC 3 cut(s) 359, 460, 1020
BstACI GRCGYC 2 cut(s) 159, 670
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 377, 394, 615
BstDEI CTNAG 2 cut(s) 12, 591
BstDSI CCRYGG 1 cut(s) 948
BstF5I GGATG 1 cut(s) 118
BstHHI GCGC 1 cut(s) 860
BstKTI GATC 2 cut(s) 328, 1041
BstMAI GTCTC 5 cut(s) 54, 816, 892, 972, 1012
BstMBI GATC 2 cut(s) 325, 1038
BstMWI GCNNNNNNNGC 5 cut(s) 14, 63, 72, 578, 587
BstNI CCWGG 6 cut(s) 619, 666, 710, 746, 920, 1088
BstNSI RCATGY 3 cut(s) 590, 617, 811
BstSCI CCNGG 8 cut(s) 300, 400, 617, 664, 708, 744, 918, 1086
BstSFI CTRYAG 3 cut(s) 6, 258, 390
BstSLI GKGCMC 2 cut(s) 466, 625
BstV1I GCAGC 5 cut(s) 20, 103, 376, 817, 931
Bsu15I ATCGAT 1 cut(s) 1037
BsuRI GGCC 3 cut(s) 66, 220, 279
BsuTUI ATCGAT 1 cut(s) 1037
BtgI CCRYGG 1 cut(s) 948
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 3 cut(s) 377, 394, 615
CfoI GCGC 1 cut(s) 860
Cfr13I GGNCC 3 cut(s) 65, 218, 742
ClaI ATCGAT 1 cut(s) 1037
CseI GACGC 1 cut(s) 678
Csp6I GTAC 2 cut(s) 398, 681
CviAII CATG 7 cut(s) 311, 587, 598, 614, 739, 808, 935
CviQI GTAC 2 cut(s) 398, 681
DdeI CTNAG 2 cut(s) 12, 591
DpnI GATC 2 cut(s) 327, 1040
DpnII GATC 2 cut(s) 325, 1038
Eam1104I CTCTTC 3 cut(s) 359, 460, 1020
EarI CTCTTC 3 cut(s) 359, 460, 1020
EciI GGCGGA 3 cut(s) 22, 32, 72
Ecl136II GAGCTC 1 cut(s) 997
Eco130I CCWWGG 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 999
Eco47I GGWCC 1 cut(s) 742
Eco53kI GAGCTC 1 cut(s) 997
Eco88I CYCGRG 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 997
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 6 cut(s) 617, 664, 708, 744, 918, 1086
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 1 cut(s) 280
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 7 cut(s) 314, 590, 601, 617, 742, 811, 938
FaqI GGGAC 1 cut(s) 969
FatI CATG 7 cut(s) 310, 586, 597, 613, 738, 807, 934
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 6 cut(s) 6, 9, 117, 390, 831, 945
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 6 cut(s) 6, 9, 117, 390, 831, 945
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 859
GluI GCNGC 6 cut(s) 6, 9, 117, 390, 831, 945
HaeIII GGCC 3 cut(s) 66, 220, 279
HapII CCGG 2 cut(s) 302, 402
HgaI GACGC 1 cut(s) 678
HhaI GCGC 1 cut(s) 860
Hin1I GRCGYC 2 cut(s) 159, 670
Hin1II CATG 7 cut(s) 314, 590, 601, 617, 742, 811, 938
Hin6I GCGC 1 cut(s) 858
HinP1I GCGC 1 cut(s) 858
HinfI GANTC 4 cut(s) 534, 782, 957, 1046
HpaII CCGG 2 cut(s) 302, 402
Hpy166II GTNNAC 2 cut(s) 502, 706
Hpy188I TCNGA 2 cut(s) 48, 941
Hpy188III TCNNGA 5 cut(s) 155, 163, 480, 767, 961
Hpy8I GTNNAC 2 cut(s) 502, 706
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 183, 476, 748, 1048
HpyCH4IV ACGT 3 cut(s) 159, 227, 414
HpyCH4V TGCA 7 cut(s) 8, 146, 192, 197, 392, 613, 929
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 63, 72, 578, 587
HpyF3I CTNAG 2 cut(s) 12, 591
HpySE526I ACGT 3 cut(s) 159, 227, 414
Hsp92I GRCGYC 2 cut(s) 159, 670
Hsp92II CATG 7 cut(s) 314, 590, 601, 617, 742, 811, 938
HspAI GCGC 1 cut(s) 858
KpnI GGTACC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 2 cut(s) 325, 1038
LguI GCTCTTC 2 cut(s) 359, 1020
Lsp1109I GCAGC 5 cut(s) 20, 103, 376, 817, 931
LweI GCATC 2 cut(s) 103, 938
MaeI CTAG 1 cut(s) 480
MaeII ACGT 3 cut(s) 159, 227, 414
MaeIII GTNAC 2 cut(s) 168, 904
MalI GATC 2 cut(s) 327, 1040
MboI GATC 2 cut(s) 325, 1038
MboII GAAGA 6 cut(s) 168, 346, 447, 690, 782, 1037
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 4 cut(s) 466, 625, 657, 999
MluCI AATT 7 cut(s) 141, 289, 305, 547, 867, 971, 1092
MlyI GAGTC 2 cut(s) 951, 1055
MseI TTAA 5 cut(s) 321, 345, 789, 1062, 1080
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 302, 402
MspR9I CCNGG 8 cut(s) 302, 402, 619, 666, 710, 746, 920, 1088
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 6 cut(s) 619, 666, 710, 746, 920, 1088
MwoI GCNNNNNNNGC 5 cut(s) 14, 63, 72, 578, 587
NciI CCSGG 2 cut(s) 302, 402
NdeII GATC 2 cut(s) 325, 1038
NlaIII CATG 7 cut(s) 314, 590, 601, 617, 742, 811, 938
NlaIV GGNNCC 2 cut(s) 67, 682
NmeAIII GCCGAG 1 cut(s) 869
NspI RCATGY 3 cut(s) 590, 617, 811
NspV TTCGAA 1 cut(s) 179
PaeI GCATGC 1 cut(s) 617
PciI ACATGT 1 cut(s) 807
PciSI GCTCTTC 2 cut(s) 359, 1020
PfeI GAWTC 2 cut(s) 534, 782
PfoI TCCNGGA 2 cut(s) 300, 664
PkrI GCNGC 6 cut(s) 7, 10, 118, 391, 832, 946
PleI GAGTC 2 cut(s) 951, 1054
PpsI GAGTC 2 cut(s) 951, 1054
PscI ACATGT 1 cut(s) 807
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 999
Psp6I CCWGG 6 cut(s) 617, 664, 708, 744, 918, 1086
PspGI CCWGG 6 cut(s) 617, 664, 708, 744, 918, 1086
PspN4I GGNNCC 2 cut(s) 67, 682
PspPI GGNCC 3 cut(s) 65, 218, 742
PstI CTGCAG 2 cut(s) 10, 394
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 399, 682
RsaNI GTAC 2 cut(s) 398, 681
SacI GAGCTC 1 cut(s) 999
SapI GCTCTTC 2 cut(s) 359, 1020
SaqAI TTAA 5 cut(s) 321, 345, 789, 1062, 1080
SatI GCNGC 6 cut(s) 6, 9, 117, 390, 831, 945
Sau3AI GATC 2 cut(s) 325, 1038
Sau96I GGNCC 3 cut(s) 65, 218, 742
SchI GAGTC 2 cut(s) 951, 1055
ScrFI CCNGG 8 cut(s) 302, 402, 619, 666, 710, 746, 920, 1088
SduI GDGCHC 4 cut(s) 466, 625, 657, 999
SfaNI GCATC 2 cut(s) 103, 938
SfcI CTRYAG 3 cut(s) 6, 258, 390
SfuI TTCGAA 1 cut(s) 179
SinI GGWCC 1 cut(s) 742
SphI GCATGC 1 cut(s) 617
Sse9I AATT 7 cut(s) 141, 289, 305, 547, 867, 971, 1092
SsiI CCGC 4 cut(s) 17, 33, 57, 236
SspMI CTAG 1 cut(s) 480
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 8 cut(s) 300, 400, 617, 664, 708, 744, 918, 1086
StyI CCWWGG 1 cut(s) 280
TaiI ACGT 3 cut(s) 162, 230, 417
TaqI TCGA 4 cut(s) 162, 179, 825, 1037
TasI AATT 7 cut(s) 141, 289, 305, 547, 867, 971, 1092
TfiI GAWTC 2 cut(s) 534, 782
Tru1I TTAA 5 cut(s) 321, 345, 789, 1062, 1080
Tru9I TTAA 5 cut(s) 321, 345, 789, 1062, 1080
TscAI CASTG 1 cut(s) 559
TseI GCWGC 6 cut(s) 5, 8, 116, 389, 830, 944
TspDTI ATGAA 5 cut(s) 65, 299, 573, 591, 767
TspGWI ACGGA 1 cut(s) 965
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 742
XapI RAATTY 4 cut(s) 289, 305, 547, 1092
XbaI TCTAGA 1 cut(s) 479
XceI RCATGY 3 cut(s) 590, 617, 811
XspI CTAG 1 cut(s) 480
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.