MD02G1312500.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000151
Physical Location & Seq
Forward (+)
36772226 .. 36775370
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1312500.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGACTGAAGTAGAGGATACTAATTTGTCTGCAGCCTATCCAATGAATGCTGGAGATGGCCCTAACAGCTATGCTAACAACTCCACTTTTCAGAAAAGTGTAGTGAATTCTTCTAAAGAAATTTTAAGACAGGAAATTGAAGAAAAGCTTGACGTACACGCATTCTTGATATCATCCTGCAACACCTTTCGAATTGCAGATTTAGGCTGCTCCACTGGGCCTAATACATTTTTTGCGGTTGAAACCATACTTGAAACTGTGCAACTCAAGTACCAAAGCCAAGGGCTGAGTTCTCATCAAATCCCCAAATTTCAAGTTTTCTTCAACGATCAAACCACAAATGATTTTAACATGCTCTTCAAATCCCTCCCTCAGAACAGGCAATACTACGCCGCAGGTGTGCCTGGTTCGTTCTATGGTAGGATATTTCCTAATGCTTCCATTCACTTTTTTCACTGTTCTTCTTCAAATCACTGGCTTTCTAGAGTACCAAAAGAGATAGTGAACAAAGAGAGTCCAGCTTGGAATAAAGGAAAAATCTATTACTCAAGTTCCACAACTGAAGTGACAAGGGCTTACGAAACTCAACATGCTTTGGACATGGAGTGTTTTCTTAATGCAAGGGCACAAGAGATTGTGTATGGAGGATTGATGGTGCTTATCATTTCATGTCGCCCCAATGGTACCCCTCATTCTCATACTCTGGCAAGTGTAATCTATGAAACTTTAGGATCTTGCCTCGTAGACATGGCCAGAAAGGGATTGGTTAATGAAGAGAAAATAGATTCATTTAACATACCTGTGTATGTCATGTCTCCCCAAGAACTGGAAGTTGCGGTAGAAAGAACTGGATACTTTAGCATTGAGAGAAAGGAAATTTTACCAAATATGTTTCCAAATAGCAATCTCTCTAATGCCTTATTATCTACATCTCACGTTAGAGCAGTTCATGAAGAACACATCAAGCAGCACTTTGGAGAAGAAATCATAGATGAAGTCTTCAACTTATACCATAAGAAAGTTGAAGAGCAACCCTCCAAGTTTGAGTTGGGGAAGACTGTTGTTTCTATTGCCGTGCTTAAACGCAACGCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

366

Amino Acids

41.17

Weight (kDa)

5.92

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 59 - 362 8.7e-104 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 386
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 683
AccB7I CCANNNNNTGG 1 cut(s) 826
AccI GTMKAC 1 cut(s) 744
AciI CCGC 3 cut(s) 236, 393, 836
AclWI GGATC 1 cut(s) 739
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 4 cut(s) 106, 120, 308, 876
AcuI CTGAAG 2 cut(s) 27, 582
AfaI GTAC 4 cut(s) 156, 272, 489, 685
AfiI CCNNNNNNNGG 1 cut(s) 826
AgsI TTSAA 9 cut(s) 140, 242, 254, 314, 325, 361, 468, 1003, 1025
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 3 cut(s) 69, 148, 521
AluI AGCT 3 cut(s) 69, 148, 521
Alw26I GTCTC 1 cut(s) 819
AlwI GGATC 1 cut(s) 739
AoxI GGCC 3 cut(s) 58, 218, 750
ApeKI GCWGC 3 cut(s) 32, 207, 967
ApoI RAATTY 4 cut(s) 106, 120, 308, 876
Asp718I GGTACC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 59, 218
AsuII TTCGAA 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 628
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BalI TGGCCA 1 cut(s) 752
BanI GGYRCC 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 991, 1061
BbvI GCAGC 3 cut(s) 44, 194, 979
BccI CCATC 2 cut(s) 50, 646
BceAI ACGGC 1 cut(s) 1058
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 2 cut(s) 10, 845
BcoDI GTCTC 1 cut(s) 819
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 30
BfuAI ACCTGC 1 cut(s) 386
BfuI GTATCC 2 cut(s) 10, 845
BisI GCNGC 4 cut(s) 33, 208, 393, 968
BlsI GCNGC 4 cut(s) 34, 209, 394, 969
Bme1390I CCNGG 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 59, 218
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 2 cut(s) 991, 1061
BplI GAGNNNNNCTC 2 cut(s) 1019, 1051
BpmI CTGGAG 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 190
BpuEI CTTGAG 2 cut(s) 251, 532
BsaJI CCNNGG 1 cut(s) 280
Bsc4I CCNNNNNNNGG 1 cut(s) 826
Bse1I ACTGG 4 cut(s) 220, 479, 831, 853
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 826
BseMII CTCAG 2 cut(s) 278, 386
BseNI ACTGG 4 cut(s) 220, 479, 831, 853
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 3 cut(s) 44, 194, 979
BshFI GGCC 3 cut(s) 60, 220, 752
BshNI GGYRCC 1 cut(s) 683
BslI CCNNNNNNNGG 1 cut(s) 826
BsmAI GTCTC 1 cut(s) 819
BsmI GAATGC 2 cut(s) 52, 161
BsnI GGCC 3 cut(s) 60, 220, 752
Bsp119I TTCGAA 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 2 cut(s) 328, 731
BspACI CCGC 3 cut(s) 236, 393, 836
BspANI GGCC 3 cut(s) 60, 220, 752
BspCNI CTCAG 2 cut(s) 279, 385
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 34
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 1 cut(s) 739
BspQI GCTCTTC 2 cut(s) 362, 1020
BspT104I TTCGAA 1 cut(s) 190
BspT107I GGYRCC 1 cut(s) 683
BsrI ACTGG 4 cut(s) 220, 479, 831, 853
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 2 cut(s) 328, 731
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 259, 458, 1060
Bst6I CTCTTC 3 cut(s) 362, 768, 1020
BstBI TTCGAA 1 cut(s) 190
BstDEI CTNAG 2 cut(s) 287, 372
BstF5I GGATG 1 cut(s) 173
BstKTI GATC 2 cut(s) 331, 734
BstMAI GTCTC 1 cut(s) 819
BstMBI GATC 2 cut(s) 328, 731
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 2 cut(s) 355, 593
BstSCI CCNGG 1 cut(s) 403
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 3 cut(s) 44, 194, 979
BstV2I GAAGAC 2 cut(s) 991, 1061
BstX2I RGATCY 1 cut(s) 731
BstYI RGATCY 1 cut(s) 731
BsuI GTATCC 2 cut(s) 10, 845
BsuRI GGCC 3 cut(s) 60, 220, 752
BtsCI GGATG 1 cut(s) 173
BtsIMutI CAGTG 3 cut(s) 213, 454, 472
BveI ACCTGC 1 cut(s) 386
CciI TCATGA 1 cut(s) 949
Cfr13I GGNCC 2 cut(s) 59, 218
Csp6I GTAC 4 cut(s) 155, 271, 488, 684
CviAII CATG 7 cut(s) 352, 590, 601, 669, 748, 811, 950
CviQI GTAC 4 cut(s) 155, 271, 488, 684
DdeI CTNAG 2 cut(s) 287, 372
DpnI GATC 2 cut(s) 330, 733
DpnII GATC 2 cut(s) 328, 731
EaeI YGGCCR 1 cut(s) 750
Eam1104I CTCTTC 3 cut(s) 362, 768, 1020
EarI CTCTTC 3 cut(s) 362, 768, 1020
Eco130I CCWWGG 1 cut(s) 280
Eco32I GATATC 1 cut(s) 171
Eco57I CTGAAG 2 cut(s) 27, 582
EcoRI GAATTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 7 cut(s) 355, 593, 604, 672, 751, 814, 953
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 956, 988
FatI CATG 7 cut(s) 351, 589, 600, 668, 747, 810, 949
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 4 cut(s) 33, 208, 393, 968
FokI GGATG 1 cut(s) 160
Fsp4HI GCNGC 4 cut(s) 33, 208, 393, 968
FspBI CTAG 1 cut(s) 483
GluI GCNGC 4 cut(s) 33, 208, 393, 968
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 3 cut(s) 60, 220, 752
Hin1II CATG 7 cut(s) 355, 593, 604, 672, 751, 814, 953
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 2 cut(s) 514, 785
Hpy166II GTNNAC 3 cut(s) 157, 505, 745
Hpy188I TCNGA 2 cut(s) 93, 375
Hpy188III TCNNGA 3 cut(s) 166, 483, 950
Hpy8I GTNNAC 3 cut(s) 157, 505, 745
HpyCH4III ACNGT 3 cut(s) 259, 458, 1060
HpyCH4IV ACGT 2 cut(s) 153, 936
HpyCH4V TGCA 5 cut(s) 32, 180, 197, 262, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 2 cut(s) 287, 372
HpySE526I ACGT 2 cut(s) 153, 936
Hsp92II CATG 7 cut(s) 355, 593, 604, 672, 751, 814, 953
KpnI GGTACC 1 cut(s) 687
Kzo9I GATC 2 cut(s) 328, 731
LguI GCTCTTC 2 cut(s) 362, 1020
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 3 cut(s) 44, 194, 979
MaeI CTAG 1 cut(s) 483
MaeII ACGT 2 cut(s) 153, 936
MaeIII GTNAC 1 cut(s) 565
MalI GATC 2 cut(s) 330, 733
MboI GATC 2 cut(s) 328, 731
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 1 cut(s) 628
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 8 cut(s) 22, 106, 120, 135, 192, 308, 876, 1093
MluNI TGGCCA 1 cut(s) 752
MlyI GAGTC 1 cut(s) 523
MnlI CCTC 7 cut(s) 7, 377, 381, 638, 699, 749, 1045
Mox20I TGGCCA 1 cut(s) 752
MscI TGGCCA 1 cut(s) 752
MseI TTAA 7 cut(s) 125, 348, 615, 768, 792, 1080, 1096
MslI CAYNNNNRTG 1 cut(s) 800
Msp20I TGGCCA 1 cut(s) 752
MspR9I CCNGG 1 cut(s) 405
Mva1269I GAATGC 2 cut(s) 52, 161
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 66
NdeII GATC 2 cut(s) 328, 731
NlaIII CATG 7 cut(s) 355, 593, 604, 672, 751, 814, 953
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 565
NspI RCATGY 2 cut(s) 355, 593
NspV TTCGAA 1 cut(s) 190
PagI TCATGA 1 cut(s) 949
PaqCI CACCTGC 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 362, 1020
PctI GAATGC 2 cut(s) 52, 161
PfeI GAWTC 1 cut(s) 785
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 4 cut(s) 34, 209, 394, 969
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 2 cut(s) 59, 218
PsrI GAACNNNNNNTAC 2 cut(s) 368, 400
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 731
RsaI GTAC 4 cut(s) 156, 272, 489, 685
RsaNI GTAC 4 cut(s) 155, 271, 488, 684
RseI CAYNNNNRTG 1 cut(s) 800
SapI GCTCTTC 2 cut(s) 362, 1020
SaqAI TTAA 7 cut(s) 125, 348, 615, 768, 792, 1080, 1096
SatI GCNGC 4 cut(s) 33, 208, 393, 968
Sau3AI GATC 2 cut(s) 328, 731
Sau96I GGNCC 2 cut(s) 59, 218
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 1 cut(s) 628
SetI ASST 8 cut(s) 71, 150, 156, 188, 400, 523, 802, 939
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 2 cut(s) 266, 547
SmoI CTYRAG 2 cut(s) 266, 547
Sse9I AATT 8 cut(s) 22, 106, 120, 135, 192, 308, 876, 1093
SsiI CCGC 3 cut(s) 236, 393, 836
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 3 cut(s) 259, 458, 1060
TaiI ACGT 2 cut(s) 156, 939
TaqI TCGA 1 cut(s) 190
TasI AATT 8 cut(s) 22, 106, 120, 135, 192, 308, 876, 1093
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 1 cut(s) 785
Tru1I TTAA 7 cut(s) 125, 348, 615, 768, 792, 1080, 1096
Tru9I TTAA 7 cut(s) 125, 348, 615, 768, 792, 1080, 1096
TscAI CASTG 3 cut(s) 220, 461, 479
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 3 cut(s) 32, 207, 967
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 8 cut(s) 59, 657, 735, 777, 786, 938, 966, 1008
TspRI CASTG 3 cut(s) 220, 461, 479
Van91I CCANNNNNTGG 1 cut(s) 826
XapI RAATTY 4 cut(s) 106, 120, 308, 876
XbaI TCTAGA 1 cut(s) 482
XceI RCATGY 2 cut(s) 355, 593
XcmI CCANNNNNNNNNTGG 2 cut(s) 760, 1045
XmiI GTMKAC 1 cut(s) 744
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.