pycom10g00420

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000151
Physical Location & Seq
Reverse (-)
461475 .. 463038
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00420.3

Sequence Viewer

Length: 1056 bp
ATGTATAATAAACTGACCATTATTCAAATAAATGTAACACCAACGTTTCTCCTTGTGCAGAGAGGAGGTGCGGATGCTTCCAAACAATTTGTAAACAAGGCAATTGCAGAGCTTGAACTAGAAACCTTGTTATCTTCCAAGACCTTTAGAATTGCAGATTTGGGTTGCTCTGTTGGCCCCAATACATTTTTTTCAGTGGAAAACGTAATTGAAGCTCTGCAGTTGAAATGCAAATCCCTAGGGTTGAATTCCCAAATACCCGAATTTCAGGTTTTCTTTAATGATCATGCCTCTAATGATTTTAACTTGCTCTTCAACTCCCTCCCACACAACCGGCAATACTATGCCGCGGGTGTGCCAGGTTCCTTCCATGGTCGGCTATTTCCTAGTAGTTCCATCCACCTTTTTCACTCTTCCTTTTCCATTCCATGGATTTCTCGAGTCCCAAAAGAGGTAGTGGATAAAAACAGCCCTGCGTGGAATAAAGGTCGAATCTTTTACTCAGATGCCACTGATGAAGTGTTAAGGGCTTATGAAGCCCAAAATGTTGAGGACATGGAGTGCTTCCTGAATGCGAGGGCACAAGAGATTGTGAATGAAGGACTCATGGTACTTATCATTCCAGGTCGCCAGGACGATACCCCTCATTCTCAATCTCTGCCGAATATGCTCTTTCAAATTTTGGGATCATGCCTCATGGAGATGGCTAGGAAGGGAATTGTTGATGAAGAGAAAGTAGATTCATTTAATATACCTAATTATTTCATGTCATCCAAAGAACTAGAAGCTGCTATGGAACGAAATGGACGCTTTAGCGTGGAAAGATGGGAAAATTTGCATCATTTTGCCGCGCATGACATTGTCTATAATATTCCTCAACTACTTGCATCTCAAGTGAGAGCTACAATGGAGGGACTCATCAAGCAGCATTTTGGAGACGAAATCTTGGACGAGCTCTTTGACTTGTATGGTAAAAGACTTGCAGAGCAACAATCCGTTGTAGTGGCAGGGAAGGCAATGGTCTATCTTGTTGTGCTTAAACGCAAGGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

352

Amino Acids

39.69

Weight (kDa)

5.72

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 429
AccII CGCG 2 cut(s) 350, 851
AciI CCGC 4 cut(s) 71, 348, 350, 849
AclI AACGTT 1 cut(s) 44
AclWI GGATC 1 cut(s) 694
AcsI RAATTY 4 cut(s) 247, 263, 678, 832
AfaI GTAC 1 cut(s) 612
AfiI CCNNNNNNNGG 2 cut(s) 429, 451
AgsI TTSAA 7 cut(s) 26, 116, 212, 226, 247, 316, 677
AjnI CCWGG 3 cut(s) 358, 622, 630
AluBI AGCT 5 cut(s) 112, 215, 788, 902, 955
AluI AGCT 5 cut(s) 112, 215, 788, 902, 955
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 788, 925
ApoI RAATTY 4 cut(s) 247, 263, 678, 832
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AspA2I CCTAGG 1 cut(s) 238
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 438
AvrII CCTAGG 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 957
BbvI GCAGC 2 cut(s) 775, 937
BccI CCATC 3 cut(s) 404, 697, 819
BciT130I CCWGG 3 cut(s) 360, 624, 632
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 930
BfaI CTAG 5 cut(s) 119, 239, 387, 708, 782
BfmI CTRYAG 1 cut(s) 218
BisI GCNGC 4 cut(s) 348, 789, 849, 926
BlnI CCTAGG 1 cut(s) 238
BlsI GCNGC 4 cut(s) 349, 790, 850, 927
Bme1390I CCNGG 3 cut(s) 360, 624, 632
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 178, 364
BmrFI CCNGG 3 cut(s) 360, 624, 632
BmsI GCATC 4 cut(s) 64, 496, 847, 896
BpuEI CTTGAG 1 cut(s) 876
BsaJI CCNNGG 4 cut(s) 238, 348, 370, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 451
Bse118I RCCGGY 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 1023
BseBI CCWGG 3 cut(s) 360, 624, 632
BseDI CCNNGG 4 cut(s) 238, 348, 370, 428
BseGI GGATG 3 cut(s) 79, 396, 770
BseLI CCNNNNNNNGG 2 cut(s) 429, 451
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 1 cut(s) 516
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 2 cut(s) 775, 937
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 2 cut(s) 350, 851
BshFI GGCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 2 cut(s) 428, 927
BslI CCNNNNNNNGG 2 cut(s) 429, 451
BsmAI GTCTC 1 cut(s) 930
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 428, 927
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 583, 957
Bsp143I GATC 2 cut(s) 283, 686
Bsp19I CCATGG 2 cut(s) 370, 428
BspACI CCGC 4 cut(s) 71, 348, 350, 849
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 515
BspFNI CGCG 2 cut(s) 350, 851
BspLI GGNNCC 2 cut(s) 178, 364
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 1 cut(s) 694
BspQI GCTCTTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 1023
BsrFI RCCGGY 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 333
BssECI CCNNGG 4 cut(s) 238, 348, 370, 428
BssMI GATC 2 cut(s) 283, 686
BssT1I CCWWGG 3 cut(s) 238, 370, 428
Bst2UI CCWGG 3 cut(s) 360, 624, 632
Bst6I CTCTTC 3 cut(s) 317, 418, 723
BstDEI CTNAG 1 cut(s) 502
BstDSI CCRYGG 3 cut(s) 348, 370, 428
BstF5I GGATG 3 cut(s) 79, 396, 770
BstFNI CGCG 2 cut(s) 350, 851
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 2 cut(s) 286, 689
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 2 cut(s) 283, 686
BstMWI GCNNNNNNNGC 4 cut(s) 174, 536, 667, 1013
BstNI CCWGG 3 cut(s) 360, 624, 632
BstSCI CCNGG 3 cut(s) 358, 622, 630
BstSFI CTRYAG 1 cut(s) 218
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 2 cut(s) 350, 851
BstV1I GCAGC 2 cut(s) 775, 937
BsuRI GGCC 1 cut(s) 177
BtgI CCRYGG 3 cut(s) 348, 370, 428
BtsCI GGATG 3 cut(s) 79, 396, 770
BtsIMutI CAGTG 2 cut(s) 201, 510
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 1 cut(s) 333
Cfr13I GGNCC 1 cut(s) 176
Cfr42I CCGCGG 1 cut(s) 351
CseI GACGC 1 cut(s) 816
Csp6I GTAC 1 cut(s) 611
CviAII CATG 9 cut(s) 287, 371, 429, 556, 607, 690, 697, 766, 854
CviQI GTAC 1 cut(s) 611
DdeI CTNAG 1 cut(s) 502
DpnI GATC 2 cut(s) 285, 688
DpnII GATC 2 cut(s) 283, 686
Eam1104I CTCTTC 3 cut(s) 317, 418, 723
EarI CTCTTC 3 cut(s) 317, 418, 723
Ecl136II GAGCTC 1 cut(s) 955
Eco130I CCWWGG 3 cut(s) 238, 370, 428
Eco24I GRGCYC 1 cut(s) 957
Eco53kI GAGCTC 1 cut(s) 955
Eco88I CYCGRG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 955
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 3 cut(s) 358, 622, 630
EcoT14I CCWWGG 3 cut(s) 238, 370, 428
EcoT38I GRGCYC 1 cut(s) 957
ErhI CCWWGG 3 cut(s) 238, 370, 428
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 9 cut(s) 290, 374, 432, 559, 610, 693, 700, 769, 857
FaqI GGGAC 2 cut(s) 428, 927
FatI CATG 9 cut(s) 286, 370, 428, 555, 606, 689, 696, 765, 853
FauI CCCGC 1 cut(s) 343
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 348, 789, 849, 926
FokI GGATG 3 cut(s) 86, 383, 757
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 4 cut(s) 348, 789, 849, 926
FspBI CTAG 5 cut(s) 119, 239, 387, 708, 782
GlaI GCGC 1 cut(s) 852
GluI GCNGC 4 cut(s) 348, 789, 849, 926
HaeIII GGCC 1 cut(s) 177
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 816
HhaI GCGC 1 cut(s) 853
Hin1II CATG 9 cut(s) 290, 374, 432, 559, 610, 693, 700, 769, 857
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HinfI GANTC 5 cut(s) 441, 492, 603, 740, 915
HpaII CCGG 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 505
Hpy188III TCNNGA 2 cut(s) 438, 568
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 4 cut(s) 376, 593, 706, 1006
HpyCH4IV ACGT 2 cut(s) 44, 204
HpyCH4V TGCA 8 cut(s) 58, 107, 155, 220, 231, 838, 887, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 536, 667, 1013
HpyF3I CTNAG 1 cut(s) 502
HpySE526I ACGT 2 cut(s) 44, 204
Hsp92II CATG 9 cut(s) 290, 374, 432, 559, 610, 693, 700, 769, 857
HspAI GCGC 1 cut(s) 851
Ksp22I TGATCA 1 cut(s) 283
KspI CCGCGG 1 cut(s) 351
Kzo9I GATC 2 cut(s) 283, 686
LguI GCTCTTC 1 cut(s) 317
Lsp1109I GCAGC 2 cut(s) 775, 937
LweI GCATC 4 cut(s) 64, 496, 847, 896
MaeI CTAG 5 cut(s) 119, 239, 387, 708, 782
MaeII ACGT 2 cut(s) 44, 204
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 285, 688
MboI GATC 2 cut(s) 283, 686
MboII GAAGA 4 cut(s) 126, 304, 405, 740
MfeI CAATTG 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 583, 957
MlyI GAGTC 3 cut(s) 450, 597, 909
MseI TTAA 5 cut(s) 279, 303, 524, 747, 1038
MslI CAYNNNNRTG 1 cut(s) 701
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 3 cut(s) 360, 624, 632
MunI CAATTG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 577
MvaI CCWGG 3 cut(s) 360, 624, 632
MvnI CGCG 2 cut(s) 350, 851
MwoI GCNNNNNNNGC 4 cut(s) 174, 536, 667, 1013
NcoI CCATGG 2 cut(s) 370, 428
NdeII GATC 2 cut(s) 283, 686
NlaIII CATG 9 cut(s) 290, 374, 432, 559, 610, 693, 700, 769, 857
NlaIV GGNNCC 2 cut(s) 178, 364
PaeR7I CTCGAG 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 317
PctI GAATGC 1 cut(s) 577
PfeI GAWTC 2 cut(s) 492, 740
PflFI GACNNNGTC 1 cut(s) 860
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 4 cut(s) 349, 790, 850, 927
PleI GAGTC 3 cut(s) 449, 597, 909
PpsI GAGTC 3 cut(s) 449, 597, 909
Psp124BI GAGCTC 1 cut(s) 957
Psp1406I AACGTT 1 cut(s) 44
Psp6I CCWGG 3 cut(s) 358, 622, 630
PspGI CCWGG 3 cut(s) 358, 622, 630
PspN4I GGNNCC 2 cut(s) 178, 364
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 222
PsyI GACNNNGTC 1 cut(s) 860
RsaI GTAC 1 cut(s) 612
RsaNI GTAC 1 cut(s) 611
RseI CAYNNNNRTG 1 cut(s) 701
SacI GAGCTC 1 cut(s) 957
SacII CCGCGG 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 5 cut(s) 279, 303, 524, 747, 1038
SatI GCNGC 4 cut(s) 348, 789, 849, 926
Sau3AI GATC 2 cut(s) 283, 686
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 3 cut(s) 450, 597, 909
ScrFI CCNGG 3 cut(s) 360, 624, 632
SduI GDGCHC 2 cut(s) 583, 957
SfaNI GCATC 4 cut(s) 64, 496, 847, 896
SfcI CTRYAG 1 cut(s) 218
Sfr274I CTCGAG 1 cut(s) 438
Sfr303I CCGCGG 1 cut(s) 351
SgrBI CCGCGG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 2 cut(s) 438, 891
SmoI CTYRAG 2 cut(s) 438, 891
SsiI CCGC 4 cut(s) 71, 348, 350, 849
SspI AATATT 1 cut(s) 871
SspMI CTAG 5 cut(s) 119, 239, 387, 708, 782
SstI GAGCTC 1 cut(s) 957
StyD4I CCNGG 3 cut(s) 358, 622, 630
StyI CCWWGG 3 cut(s) 238, 370, 428
TaiI ACGT 2 cut(s) 47, 207
TaqI TCGA 2 cut(s) 439, 490
TauI GCSGC 2 cut(s) 350, 851
TfiI GAWTC 2 cut(s) 492, 740
Tru1I TTAA 5 cut(s) 279, 303, 524, 747, 1038
Tru9I TTAA 5 cut(s) 279, 303, 524, 747, 1038
TscAI CASTG 2 cut(s) 201, 517
TseI GCWGC 2 cut(s) 788, 925
TspDTI ATGAA 6 cut(s) 531, 549, 612, 732, 741, 754
TspGWI ACGGA 1 cut(s) 985
TspRI CASTG 2 cut(s) 201, 517
Tth111I GACNNNGTC 1 cut(s) 860
Van91I CCANNNNNTGG 1 cut(s) 429
XapI RAATTY 4 cut(s) 247, 263, 678, 832
XhoI CTCGAG 1 cut(s) 438
XmaJI CCTAGG 1 cut(s) 238
XspI CTAG 5 cut(s) 119, 239, 387, 708, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.