Rw2G027140

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000151
Physical Location & Seq
Forward (+)
42268901 .. 42270911
2011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027140.1

Sequence Viewer

Length: 645 bp
ATGGCCATAGAGGAACTCAGCAAAATGGTTGAAGCATATCCAATGAAAGGTGGAGATGGACCCAACAGCTATGCCAAGAACTCCAGTTACCAGAAAGGAGTTGCAGGTGCTGCCCAAGAAGTTGTGAACAGGGCTATAACAGAAAAGCTTGACATAGACAATATCACATTATCTAATTCCAACACCTTTAGCATTGCAGATTTAGGTTGCTCTGTTGGGCCAAATACGTTTTCTGCAGTTGGGAACATAATTGAAGCTGTGCAGCTCAAGTATCAAAGCCAAGTGCGGCTGCTTGATTCCCAAATCCCTGAATTTCAAGTTTTCTTTAATGATCATACCCCAAATGACTTTAACATGCTCTTCCAGTCCCTCCCTCCCAACAGACAATACTATGCAATGGGTGTGCCCGGTTCTTTCTATGGTCGTCTATTTCCTAAAGCTTCGGTTCACTTAGTTCACTCTTCTTACGCCATTCATTGGCTTTCAAGGGTACCGAAGCAGGGACTGTTCAGGCAGCAATTTGGAGATGAAATTTTAGATGAGCTCTTTGACTTGTACCGCAAGAAACTTGAAGAGAATCCCTCTATCTTTGAGTCAATGGATGCAACTCTCTTTCTTGCCGTGCTTAAACGCAACGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

214

Amino Acids

24.0

Weight (kDa)

5.57

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 56 - 167 2.3e-47 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 477
AciI CCGC 2 cut(s) 286, 559
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 311, 531
AfaI GTAC 2 cut(s) 492, 557
AfiI CCNNNNNNNGG 3 cut(s) 47, 477, 500
AgsI TTSAA 5 cut(s) 32, 254, 317, 486, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 214, 246
AluBI AGCT 6 cut(s) 69, 148, 257, 265, 440, 544
AluI AGCT 6 cut(s) 69, 148, 257, 265, 440, 544
Alw21I GWGCWC 1 cut(s) 546
AlwNI CAGNNNCTG 2 cut(s) 110, 505
AoxI GGCC 2 cut(s) 3, 218
ApeKI GCWGC 4 cut(s) 110, 262, 289, 514
ApoI RAATTY 2 cut(s) 311, 531
Asp718I GGTACC 1 cut(s) 490
AspS9I GGNCC 2 cut(s) 59, 218
AsuC2I CCSGG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 408
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 4 cut(s) 97, 274, 276, 526
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 605
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 5 cut(s) 111, 263, 287, 290, 515
BlsI GCNGC 5 cut(s) 112, 264, 288, 291, 516
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 218
BmiI GGNNCC 2 cut(s) 61, 492
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 1 cut(s) 592
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 408
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 477, 500
Bse1I ACTGG 2 cut(s) 84, 364
Bse3DI GCAATG 2 cut(s) 192, 402
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 3 cut(s) 47, 477, 500
BseMI GCAATG 2 cut(s) 192, 402
BseMII CTCAG 1 cut(s) 31
BseNI ACTGG 2 cut(s) 84, 364
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 4 cut(s) 97, 274, 276, 526
BsgI GTGCAG 1 cut(s) 281
BshFI GGCC 2 cut(s) 5, 220
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 2 cut(s) 352, 516
BslI CCNNNNNNNGG 3 cut(s) 47, 477, 500
BsmFI GGGAC 2 cut(s) 352, 516
BsnI GGCC 2 cut(s) 5, 220
Bsp1286I GDGCHC 2 cut(s) 408, 546
Bsp143I GATC 1 cut(s) 331
BspACI CCGC 2 cut(s) 286, 559
BspANI GGCC 2 cut(s) 5, 220
BspCNI CTCAG 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 61, 492
BspMAI CTGCAG 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 2 cut(s) 192, 402
BsrI ACTGG 2 cut(s) 84, 364
BssMI GATC 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 507
Bst6I CTCTTC 3 cut(s) 365, 466, 567
BstAPI GCANNNNNTGC 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 17, 451
BstF5I GGATG 1 cut(s) 607
BstKTI GATC 1 cut(s) 334
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 4 cut(s) 97, 274, 276, 526
BsuRI GGCC 2 cut(s) 5, 220
BtsCI GGATG 1 cut(s) 607
BveI ACCTGC 1 cut(s) 95
CaiI CAGNNNCTG 2 cut(s) 110, 505
Cfr13I GGNCC 2 cut(s) 59, 218
Csp6I GTAC 2 cut(s) 491, 556
CviAII CATG 1 cut(s) 355
CviQI GTAC 2 cut(s) 491, 556
DdeI CTNAG 2 cut(s) 17, 451
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 365, 466, 567
EarI CTCTTC 3 cut(s) 365, 466, 567
Ecl136II GAGCTC 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 546
FaeI CATG 1 cut(s) 358
FaqI GGGAC 2 cut(s) 352, 516
FatI CATG 1 cut(s) 354
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 5 cut(s) 111, 263, 287, 290, 515
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 5 cut(s) 111, 263, 287, 290, 515
GluI GCNGC 5 cut(s) 111, 263, 287, 290, 515
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 2 cut(s) 5, 220
HapII CCGG 1 cut(s) 408
Hin1II CATG 1 cut(s) 358
HindIII AAGCTT 2 cut(s) 146, 438
HinfI GANTC 3 cut(s) 296, 577, 593
HpaII CCGG 1 cut(s) 408
Hpy166II GTNNAC 3 cut(s) 127, 448, 457
Hpy8I GTNNAC 3 cut(s) 127, 448, 457
HpyCH4III ACNGT 1 cut(s) 507
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 6 cut(s) 104, 197, 236, 262, 395, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 17, 451
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 1 cut(s) 358
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 1 cut(s) 365
LpnPI CCDG 9 cut(s) 90, 97, 104, 115, 321, 377, 421, 485, 496
Lsp1109I GCAGC 4 cut(s) 97, 274, 276, 526
LweI GCATC 1 cut(s) 592
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 3 cut(s) 352, 453, 584
MhlI GDGCHC 2 cut(s) 408, 546
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 175, 249, 311, 518, 531, 640
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 4 cut(s) 4, 380, 384, 592
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 327, 351, 627
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 110
NciI CCSGG 1 cut(s) 408
NdeII GATC 1 cut(s) 331
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 2 cut(s) 61, 492
NspI RCATGY 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 296, 577
PflMI CCANNNNNTGG 1 cut(s) 477
PkrI GCNGC 5 cut(s) 112, 264, 288, 291, 516
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 546
PspN4I GGNNCC 2 cut(s) 61, 492
PspPI GGNCC 2 cut(s) 59, 218
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 2 cut(s) 110, 505
RsaI GTAC 2 cut(s) 492, 557
RsaNI GTAC 2 cut(s) 491, 556
SacI GAGCTC 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 3 cut(s) 327, 351, 627
SatI GCNGC 5 cut(s) 111, 263, 287, 290, 515
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 2 cut(s) 59, 218
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 2 cut(s) 408, 546
SfaNI GCATC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 6 cut(s) 175, 249, 311, 518, 531, 640
SsiI CCGC 2 cut(s) 286, 559
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 507
TaiI ACGT 1 cut(s) 230
TasI AATT 6 cut(s) 175, 249, 311, 518, 531, 640
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 2 cut(s) 296, 577
Tru1I TTAA 3 cut(s) 327, 351, 627
Tru9I TTAA 3 cut(s) 327, 351, 627
TseI GCWGC 4 cut(s) 110, 262, 289, 514
TspDTI ATGAA 3 cut(s) 59, 464, 543
Van91I CCANNNNNTGG 1 cut(s) 477
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 2 cut(s) 311, 531
XceI RCATGY 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.