Rorug04G0027400

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000151
Physical Location & Seq
Reverse (-)
3804129 .. 3813330
9202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0027400.1

Sequence Viewer

Length: 603 bp
ATGAAGGTCAAAACACCCACCCTTTTTCTGTTGGGTGCAAAGGATCTTCGTGTTCCGATTTCCACTGGATTGCAGTATGCTCGGGCATTGAAGGAAAAAGGAGTTGCTGTCAAAGTCATTGTGTTCCCTAATGACATCCATCCAATCGACAGGCCTCAATCCGACTTTGAGAGCTTTCTTAATATTGGCGTGTGGTTTAAGAATTCACGCGGAGGTTTTGCCAGGTTAGGTGGCTTGTGGTGTGCTGGGGGTCGATCTCACAAGGAAAGGGAGGAGCAGGTGTTGTCGCTGCTTGGTGGTGCGAGTATGGGTTCTGACCTTCTCTGGTCGTGGACGAGCTCAGGGATGAAGCGGCGACGATGGGTTGCTGGCCGGCGGCGAGAGCTGGGGTCTCTGTCTCGGTCTGGCCTTCTCGGTTTGAGGAAGTGCAGCGGGTCTAAGGTGCTGAAGCGTGGTCCACGGTGGGAGGAGGCTGGTTCGATCTGGGTTTGGGTGGATCTGGATTCGAATTGGGTTTGGGTTTGGACGGGAGTCTGCTGGTGTAGTTCCGTGTTGTTTCTGGGGCAGTTGGAGTGGCTCTGGGACACGGATGGCCGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

200

Amino Acids

22.63

Weight (kDa)

10.08

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 3 - 68 5.2e-08 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
Acc36I ACCTGC 1 cut(s) 268
AccII CGCG 1 cut(s) 210
AciI CCGC 4 cut(s) 210, 352, 376, 432
AclWI GGATC 2 cut(s) 51, 504
AcoI YGGCCR 2 cut(s) 370, 592
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 467
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 3 cut(s) 174, 339, 385
AluI AGCT 3 cut(s) 174, 339, 385
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 396, 402
AlwI GGATC 2 cut(s) 51, 504
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 4 cut(s) 152, 370, 406, 592
ApeKI GCWGC 2 cut(s) 289, 429
ApoI RAATTY 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 1 cut(s) 455
BanII GRGCYC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 276, 441
BccI CCATC 3 cut(s) 147, 354, 584
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 396, 402
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 290, 353, 377, 430
BlsI GCNGC 4 cut(s) 291, 354, 378, 431
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 223
BoxI GACNNNNGTC 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 340
Bpu14I TTCGAA 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 458
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 266, 296
Bse118I RCCGGY 2 cut(s) 372, 594
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 458
BseGI GGATG 4 cut(s) 135, 139, 351, 595
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 2 cut(s) 287, 482
BseXI GCAGC 2 cut(s) 276, 441
BseYI CCCAGC 2 cut(s) 245, 385
BsgI GTGCAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 4 cut(s) 154, 372, 408, 594
BsiHKAI GWGCWC 1 cut(s) 341
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 373, 595
BslFI GGGAC 1 cut(s) 596
BsmAI GTCTC 2 cut(s) 396, 402
BsmFI GGGAC 1 cut(s) 596
BsnI GGCC 4 cut(s) 154, 372, 408, 594
Bso31I GGTCTC 1 cut(s) 396
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 4 cut(s) 43, 254, 480, 496
BspACI CCGC 4 cut(s) 210, 352, 376, 432
BspANI GGCC 4 cut(s) 154, 372, 408, 594
BspCNI CTCAG 1 cut(s) 353
BspFNI CGCG 1 cut(s) 210
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 2 cut(s) 51, 504
BspT104I TTCGAA 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 396
BsrFI RCCGGY 2 cut(s) 372, 594
BsrI ACTGG 1 cut(s) 70
BssAI RCCGGY 2 cut(s) 372, 594
BssECI CCNNGG 1 cut(s) 458
BssMI GATC 4 cut(s) 43, 254, 480, 496
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 1 cut(s) 462
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 370, 374
BstDEI CTNAG 2 cut(s) 340, 438
BstDSI CCRYGG 1 cut(s) 458
BstF5I GGATG 4 cut(s) 135, 139, 351, 595
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 4 cut(s) 46, 257, 483, 499
BstMAI GTCTC 2 cut(s) 396, 402
BstMBI GATC 4 cut(s) 43, 254, 480, 496
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstNI CCWGG 1 cut(s) 223
BstPAI GACNNNNGTC 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 221
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 276, 441
BstX2I RGATCY 2 cut(s) 43, 496
BstYI RGATCY 2 cut(s) 43, 496
BsuRI GGCC 4 cut(s) 154, 372, 408, 594
BtgI CCRYGG 1 cut(s) 458
BtsCI GGATG 4 cut(s) 135, 139, 351, 595
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 370, 374
Cfr10I RCCGGY 2 cut(s) 372, 594
Cfr13I GGNCC 1 cut(s) 455
DdeI CTNAG 2 cut(s) 340, 438
DpnI GATC 4 cut(s) 45, 256, 482, 498
DpnII GATC 4 cut(s) 43, 254, 480, 496
EaeI YGGCCR 2 cut(s) 370, 592
Ecl136II GAGCTC 1 cut(s) 339
Eco147I AGGCCT 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 339
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 341
FaiI YATR 2 cut(s) 78, 308
FaqI GGGAC 1 cut(s) 596
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 4 cut(s) 290, 353, 377, 430
FokI GGATG 3 cut(s) 122, 126, 358
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 290, 353, 377, 430
GluI GCNGC 4 cut(s) 290, 353, 377, 430
GsaI CCCAGC 2 cut(s) 249, 389
HaeIII GGCC 4 cut(s) 154, 372, 408, 594
HapII CCGG 2 cut(s) 373, 595
HinfI GANTC 2 cut(s) 503, 531
HpaII CCGG 2 cut(s) 373, 595
Hpy166II GTNNAC 2 cut(s) 333, 458
Hpy188I TCNGA 3 cut(s) 57, 163, 316
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 2 cut(s) 333, 458
Hpy99I CGWCG 1 cut(s) 360
HpyAV CCTTC 3 cut(s) 85, 329, 419
HpyCH4III ACNGT 1 cut(s) 462
HpyCH4V TGCA 3 cut(s) 38, 73, 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 2 cut(s) 340, 438
KroI GCCGGC 1 cut(s) 372
KroNI GCCGGC 1 cut(s) 374
Kzo9I GATC 4 cut(s) 43, 254, 480, 496
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 2 cut(s) 276, 441
MalI GATC 4 cut(s) 45, 256, 482, 498
MboI GATC 4 cut(s) 43, 254, 480, 496
MboII GAAGA 1 cut(s) 38
MflI RGATCY 2 cut(s) 43, 496
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 2 cut(s) 202, 508
MlyI GAGTC 1 cut(s) 540
MmeI TCCRAC 2 cut(s) 186, 549
MnlI CCTC 6 cut(s) 165, 206, 265, 414, 460, 463
MroNI GCCGGC 1 cut(s) 372
MseI TTAA 2 cut(s) 180, 198
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 2 cut(s) 373, 595
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 382
NaeI GCCGGC 1 cut(s) 374
NdeII GATC 4 cut(s) 43, 254, 480, 496
NgoMIV GCCGGC 1 cut(s) 372
NspV TTCGAA 1 cut(s) 506
PaqCI CACCTGC 1 cut(s) 268
PceI AGGCCT 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 4 cut(s) 291, 354, 378, 431
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
PshAI GACNNNNGTC 1 cut(s) 530
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 221
PspFI CCCAGC 2 cut(s) 245, 385
PspGI CCWGG 1 cut(s) 221
PspPI GGNCC 1 cut(s) 455
PsuI RGATCY 2 cut(s) 43, 496
SacI GAGCTC 1 cut(s) 341
SaqAI TTAA 2 cut(s) 180, 198
SatI GCNGC 4 cut(s) 290, 353, 377, 430
Sau3AI GATC 4 cut(s) 43, 254, 480, 496
Sau96I GGNCC 1 cut(s) 455
SchI GAGTC 1 cut(s) 540
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 341
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 1 cut(s) 455
Sse9I AATT 2 cut(s) 202, 508
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 4 cut(s) 210, 352, 376, 432
SspI AATATT 1 cut(s) 184
SstI GAGCTC 1 cut(s) 341
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 462
TaqI TCGA 4 cut(s) 147, 253, 479, 506
TaqII GACCGA 1 cut(s) 390
TasI AATT 2 cut(s) 202, 508
TauI GCSGC 2 cut(s) 355, 379
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 180, 198
Tru9I TTAA 2 cut(s) 180, 198
TscAI CASTG 1 cut(s) 70
TseI GCWGC 2 cut(s) 289, 429
TspDTI ATGAA 2 cut(s) 17, 362
TspGWI ACGGA 2 cut(s) 538, 602
TspRI CASTG 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.