RLG00000005391

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000151
Physical Location & Seq
Reverse (-)
67268671 .. 67279178
10508 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005391

Sequence Viewer

Length: 1287 bp
ATGATTGGGGACGAGGTCGGCGAGTCGGCTGTGCAGGGTTTGGGGTTGGGGACTGTTGAAAATCCAGAGAGGGGGCCGCACCCAGACGCCGACGAAGAACAGCCTGGGCCTTTCCAATTCCACTTTCACGAAAACCAAATCGAGCAAAAAGCGACGGGACGCGGCTGTGGAGGCAGAGTCGGACGCCATATCGATTCTAAACGAGAGAATTCGGCCCGAGCAGAGAAAGAAATCGCCGGAGGGGTCTCCGATGGAGGCGGAGGAGGCAGACAAGTACTTGGATCGAGCTCCGGCGCCGCCACTCTGGTCCGCGTCGACTCCGACGACGACGTCCGACGCATGGTCGAAGCGTTCGAGAAGCTCGAGAGCTACGAACCAATGGAATGTGGAGATGATAGCGCCAAGAACTCCACTATGCTGAAAGGAGTTTTGGCTGTGGCCAAAGAACTTATAAGAAAGGCAATTGCAGAAAAGCTTGACATAGACATCTTGCCACCTTCCACCTCCTTCAAAATTGCAGACCTCGGTTGCTCTGTTGGACCCAATACATTTTTCGCAGTTGAAAACATAGTTGAAGCTGTGGAACTCAAGTACCAAGGGCAAGGGGTGAATTCTCAAATCCCTAAATTTCAAGTATTCTTTAATGATCATACCTCAAATGACTTTAACATGCTCTTCAAGTCCCTCCCTCAGAACAGGCGATACTATGCAGCTGGTGTGCCGGGTTCTTTCTATGCTCGGGTATTTCCTAATGCTTCCATCTACGTTGTTCACTCTTCTTTAGCCATTCAATGGCTTTCTAGAGTACCAAAAGAGGTATTGGATAGAAACAGTCCTGCTTGGAATAAAGGACACATCTGTTACTCAAACTCCACAGATGAAGTAGCGAGGGCTTACAAAGCTCAATATGCCGAGGACATGGAATGCTTCCTGCATGCTAGGGCGCAAGAAATTGGAGTTGTTAGCGAAGAGAAGGTGGATTCATTTAACATGCCTATGTATTCCATGTCACCTCAAGAACTTGAAGTTGCTGTCAAACGAAATGGATGCTTTAGCATAGACATAATGGCAGAGTTACCTCGTCCCTTGGTACACGATACTCTTTCAATATCCCAAATGATTGCTTCTCACATAAGAGCTGGTATGGAGGGGATGATAAAGAAGCAATTTGGAGAAGAAATCTTAGATGAGCTTTTCGACTTGTACCAACAAAAATGTGAACAATCGGTAATTAGCACTCTGGTTGGACACACCTTTCTTGTTGTTCTTAGACGTAAAGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

429

Amino Acids

47.22

Weight (kDa)

5.24

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 159 - 318 5.2e-62 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 319 - 424 6.8e-21 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AasI GACNNNNNNGTC 1 cut(s) 329
AatII GACGTC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 792
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 162, 312
AciI CCGC 5 cut(s) 77, 162, 258, 297, 310
AclWI GGATC 1 cut(s) 289
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 3 cut(s) 208, 610, 626
AcyI GRCGYC 4 cut(s) 87, 184, 294, 330
AfaI GTAC 5 cut(s) 276, 593, 807, 1092, 1205
AfiI CCNNNNNNNGG 3 cut(s) 71, 340, 792
AgsI TTSAA 9 cut(s) 59, 511, 563, 575, 632, 679, 791, 1025, 1107
AhdI GACNNNNNGTC 1 cut(s) 341
AjnI CCWGG 1 cut(s) 103
AjuI GAANNNNNNNTTGG 6 cut(s) 108, 140, 413, 445, 536, 568
AluBI AGCT 9 cut(s) 288, 361, 369, 475, 578, 713, 902, 1139, 1192
AluI AGCT 9 cut(s) 288, 361, 369, 475, 578, 713, 902, 1139, 1192
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 1 cut(s) 289
Ama87I CYCGRG 3 cut(s) 216, 362, 738
AoxI GGCC 4 cut(s) 74, 107, 213, 438
ApeKI GCWGC 1 cut(s) 710
ApoI RAATTY 3 cut(s) 208, 610, 626
AspLEI GCGC 3 cut(s) 296, 401, 946
AspS9I GGNCC 5 cut(s) 74, 107, 214, 307, 539
AsuC2I CCSGG 1 cut(s) 723
AsuHPI GGTGA 2 cut(s) 619, 1002
AvaI CYCGRG 3 cut(s) 216, 362, 738
AvaII GGWCC 2 cut(s) 307, 539
BaeI ACNNNNGTAYC 2 cut(s) 575, 608
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 722
BccI CCATC 2 cut(s) 245, 767
BcgI CGANNNNNNTGC 2 cut(s) 1029, 1063
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 1 cut(s) 646
BcnI CCSGG 1 cut(s) 723
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 2 cut(s) 801, 939
BfoI RGCGCY 2 cut(s) 297, 402
BisI GCNGC 4 cut(s) 77, 163, 297, 711
BlsI GCNGC 4 cut(s) 78, 164, 298, 712
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 105, 723
Bme18I GGWCC 2 cut(s) 307, 539
BmeRI GACNNNNNGTC 1 cut(s) 341
BmeT110I CYCGRG 3 cut(s) 216, 362, 738
BmgT120I GGNCC 5 cut(s) 74, 107, 214, 307, 539
BmiI GGNNCC 3 cut(s) 75, 295, 541
BmrFI CCNGG 2 cut(s) 105, 723
BmsI GCATC 1 cut(s) 1037
BpuEI CTTGAG 2 cut(s) 572, 999
BpuMI CCSGG 1 cut(s) 723
Bsa29I ATCGAT 1 cut(s) 192
BsaHI GRCGYC 4 cut(s) 87, 184, 294, 330
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 5 cut(s) 104, 523, 595, 912, 1086
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 854, 884
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 340, 792
BseBI CCWGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 5 cut(s) 104, 523, 595, 912, 1086
BseGI GGATG 2 cut(s) 1052, 1158
BseLI CCNNNNNNNGG 3 cut(s) 71, 340, 792
BseMII CTCAG 1 cut(s) 704
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 722
BsgI GTGCAG 1 cut(s) 53
Bsh1236I CGCG 2 cut(s) 162, 312
BshFI GGCC 4 cut(s) 76, 109, 215, 440
BshNI GGYRCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 3 cut(s) 216, 362, 738
BsiSI CCGG 3 cut(s) 237, 291, 722
BslFI GGGAC 5 cut(s) 23, 64, 171, 667, 1068
BslI CCNNNNNNNGG 3 cut(s) 71, 340, 792
BsmAI GTCTC 1 cut(s) 250
BsmFI GGGAC 5 cut(s) 23, 64, 171, 667, 1068
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 4 cut(s) 76, 109, 215, 440
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 3 cut(s) 216, 362, 738
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 2 cut(s) 281, 646
BspACI CCGC 5 cut(s) 77, 162, 258, 297, 310
BspANI GGCC 4 cut(s) 76, 109, 215, 440
BspCNI CTCAG 1 cut(s) 703
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 2 cut(s) 162, 312
BspLI GGNNCC 3 cut(s) 75, 295, 541
BspPI GGATC 1 cut(s) 289
BspQI GCTCTTC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 250
BssECI CCNNGG 5 cut(s) 104, 523, 595, 912, 1086
BssMI GATC 2 cut(s) 281, 646
BssNI GRCGYC 4 cut(s) 87, 184, 294, 330
BssT1I CCWWGG 2 cut(s) 595, 1086
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 55, 833
Bst6I CTCTTC 3 cut(s) 680, 781, 963
BstACI GRCGYC 4 cut(s) 87, 184, 294, 330
BstC8I GCNNGC 1 cut(s) 936
BstDEI CTNAG 3 cut(s) 690, 1183, 1268
BstF5I GGATG 2 cut(s) 1052, 1158
BstFNI CGCG 2 cut(s) 162, 312
BstH2I RGCGCY 2 cut(s) 297, 402
BstHHI GCGC 3 cut(s) 296, 401, 946
BstKTI GATC 2 cut(s) 284, 649
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 2 cut(s) 281, 646
BstMWI GCNNNNNNNGC 4 cut(s) 171, 264, 899, 908
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 3 cut(s) 673, 938, 994
BstSCI CCNGG 2 cut(s) 103, 721
BstUI CGCG 2 cut(s) 162, 312
BstV1I GCAGC 1 cut(s) 722
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 4 cut(s) 76, 109, 215, 440
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 2 cut(s) 1052, 1158
Cac8I GCNNGC 1 cut(s) 936
CfoI GCGC 3 cut(s) 296, 401, 946
Cfr13I GGNCC 5 cut(s) 74, 107, 214, 307, 539
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 5 cut(s) 95, 168, 192, 301, 345
Csp6I GTAC 5 cut(s) 275, 592, 806, 1091, 1204
CviAII CATG 6 cut(s) 340, 670, 919, 935, 991, 1006
CviQI GTAC 5 cut(s) 275, 592, 806, 1091, 1204
DdeI CTNAG 3 cut(s) 690, 1183, 1268
DinI GGCGCC 1 cut(s) 295
DpnI GATC 2 cut(s) 283, 648
DpnII GATC 2 cut(s) 281, 646
DrdI GACNNNNNNGTC 1 cut(s) 329
DriI GACNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 3 cut(s) 680, 781, 963
Eam1105I GACNNNNNGTC 1 cut(s) 341
EarI CTCTTC 3 cut(s) 680, 781, 963
EciI GGCGGA 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 595, 1086
Eco24I GRGCYC 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 307, 539
Eco53kI GAGCTC 1 cut(s) 288
Eco88I CYCGRG 3 cut(s) 216, 362, 738
EcoICRI GAGCTC 1 cut(s) 288
EcoRI GAATTC 2 cut(s) 208, 610
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 595, 1086
EcoT38I GRGCYC 1 cut(s) 290
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 2 cut(s) 595, 1086
FaeI CATG 6 cut(s) 343, 673, 922, 938, 994, 1009
FaqI GGGAC 5 cut(s) 23, 64, 171, 667, 1068
FatI CATG 6 cut(s) 339, 669, 918, 934, 990, 1005
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 77, 163, 297, 711
FokI GGATG 2 cut(s) 1059, 1165
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 4 cut(s) 77, 163, 297, 711
FspBI CTAG 2 cut(s) 801, 939
GlaI GCGC 3 cut(s) 295, 400, 945
GluI GCNGC 4 cut(s) 77, 163, 297, 711
HaeII RGCGCY 2 cut(s) 297, 402
HaeIII GGCC 4 cut(s) 76, 109, 215, 440
HapII CCGG 3 cut(s) 237, 291, 722
HgaI GACGC 5 cut(s) 95, 168, 192, 301, 345
HhaI GCGC 3 cut(s) 296, 401, 946
Hin1I GRCGYC 4 cut(s) 87, 184, 294, 330
Hin1II CATG 6 cut(s) 343, 673, 922, 938, 994, 1009
Hin6I GCGC 3 cut(s) 294, 399, 944
HinP1I GCGC 3 cut(s) 294, 399, 944
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 5 cut(s) 23, 177, 194, 317, 980
HpaII CCGG 3 cut(s) 237, 291, 722
HphI GGTGA 2 cut(s) 619, 1002
Hpy166II GTNNAC 4 cut(s) 316, 772, 1093, 1220
Hpy188I TCNGA 5 cut(s) 182, 250, 322, 335, 693
Hpy188III TCNNGA 6 cut(s) 65, 128, 355, 364, 801, 1016
Hpy8I GTNNAC 4 cut(s) 316, 772, 1093, 1220
Hpy99I CGWCG 7 cut(s) 95, 157, 317, 326, 329, 332, 339
HpyAV CCTTC 3 cut(s) 507, 517, 967
HpyCH4III ACNGT 2 cut(s) 55, 833
HpyCH4IV ACGT 3 cut(s) 330, 765, 1273
HpyCH4V TGCA 5 cut(s) 34, 467, 518, 710, 934
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 264, 899, 908
HpyF3I CTNAG 3 cut(s) 690, 1183, 1268
HpySE526I ACGT 3 cut(s) 330, 765, 1273
Hsp92I GRCGYC 4 cut(s) 87, 184, 294, 330
Hsp92II CATG 6 cut(s) 343, 673, 922, 938, 994, 1009
HspAI GCGC 3 cut(s) 294, 399, 944
KasI GGCGCC 1 cut(s) 293
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 2 cut(s) 281, 646
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 1 cut(s) 722
LweI GCATC 1 cut(s) 1037
MaeI CTAG 2 cut(s) 801, 939
MaeII ACGT 3 cut(s) 330, 765, 1273
MaeIII GTNAC 3 cut(s) 860, 1008, 1074
MalI GATC 2 cut(s) 283, 648
MboI GATC 2 cut(s) 281, 646
MboII GAAGA 5 cut(s) 107, 667, 768, 980, 1187
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 290
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 9 cut(s) 116, 208, 462, 513, 610, 626, 951, 1166, 1230
MluNI TGGCCA 1 cut(s) 440
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 3 cut(s) 32, 186, 311
MmeI TCCRAC 5 cut(s) 160, 345, 358, 517, 1225
Mox20I TGGCCA 1 cut(s) 440
MscI TGGCCA 1 cut(s) 440
MseI TTAA 3 cut(s) 642, 666, 987
MslI CAYNNNNRTG 1 cut(s) 995
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 3 cut(s) 237, 291, 722
MspR9I CCNGG 2 cut(s) 105, 723
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 2 cut(s) 162, 312
MwoI GCNNNNNNNGC 4 cut(s) 171, 264, 899, 908
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 1 cut(s) 723
NdeII GATC 2 cut(s) 281, 646
NlaIII CATG 6 cut(s) 343, 673, 922, 938, 994, 1009
NlaIV GGNNCC 3 cut(s) 75, 295, 541
NmeAIII GCCGAG 1 cut(s) 937
NmuCI GTSAC 1 cut(s) 1008
NspI RCATGY 3 cut(s) 673, 938, 994
PaeI GCATGC 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 680
PcsI WCGNNNNNNNCGW 5 cut(s) 18, 321, 351, 360, 369
PctI GAATGC 1 cut(s) 929
PfeI GAWTC 2 cut(s) 194, 980
PflFI GACNNNGTC 2 cut(s) 14, 329
PflMI CCANNNNNTGG 1 cut(s) 792
PkrI GCNGC 4 cut(s) 78, 164, 298, 712
PleI GAGTC 3 cut(s) 31, 185, 311
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 3 cut(s) 31, 185, 311
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 3 cut(s) 75, 295, 541
PspPI GGNCC 5 cut(s) 74, 107, 214, 307, 539
PsrI GAACNNNNNNTAC 2 cut(s) 686, 718
PsyI GACNNNGTC 2 cut(s) 14, 329
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 5 cut(s) 276, 593, 807, 1092, 1205
RsaNI GTAC 5 cut(s) 275, 592, 806, 1091, 1204
RseI CAYNNNNRTG 1 cut(s) 995
SacI GAGCTC 1 cut(s) 290
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 642, 666, 987
SatI GCNGC 4 cut(s) 77, 163, 297, 711
Sau3AI GATC 2 cut(s) 281, 646
Sau96I GGNCC 5 cut(s) 74, 107, 214, 307, 539
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 3 cut(s) 32, 186, 311
ScrFI CCNGG 2 cut(s) 105, 723
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 1 cut(s) 1037
SfoI GGCGCC 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 362
SinI GGWCC 2 cut(s) 307, 539
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 3 cut(s) 362, 587, 1014
SmoI CTYRAG 3 cut(s) 362, 587, 1014
SphI GCATGC 1 cut(s) 938
Sse9I AATT 9 cut(s) 116, 208, 462, 513, 610, 626, 951, 1166, 1230
SsiI CCGC 5 cut(s) 77, 162, 258, 297, 310
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 2 cut(s) 801, 939
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 103, 721
StyI CCWWGG 2 cut(s) 595, 1086
TaaI ACNGT 2 cut(s) 55, 833
TaiI ACGT 3 cut(s) 333, 768, 1276
TaqI TCGA 8 cut(s) 141, 192, 284, 315, 345, 354, 363, 1197
TasI AATT 9 cut(s) 116, 208, 462, 513, 610, 626, 951, 1166, 1230
TatI WGTACW 1 cut(s) 274
TauI GCSGC 3 cut(s) 79, 165, 299
TfiI GAWTC 2 cut(s) 194, 980
Tru1I TTAA 3 cut(s) 642, 666, 987
Tru9I TTAA 3 cut(s) 642, 666, 987
TseFI GTSAC 1 cut(s) 1008
TseI GCWGC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 1008
TspDTI ATGAA 2 cut(s) 894, 972
Tth111I GACNNNGTC 2 cut(s) 14, 329
Van91I CCANNNNNTGG 1 cut(s) 792
VpaK11BI GGWCC 2 cut(s) 307, 539
XapI RAATTY 3 cut(s) 208, 610, 626
XbaI TCTAGA 1 cut(s) 800
XceI RCATGY 3 cut(s) 673, 938, 994
XhoI CTCGAG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 2 cut(s) 801, 939
ZraI GACGTC 1 cut(s) 331
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.