MD06G1223600.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000151
Physical Location & Seq
Reverse (-)
35450508 .. 35452232
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1223600.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCAGCACCGGAAGCCAGCACATTCTCAGAAGCATTTTCAATGAAAGGTGGAGATGGCTCCGATGGCTATGCGAAAAACTCAACGTTCCAGAGAGGAGTTGTGGATGCTACCAAAGAACTTCTAAGCAAGGCAATTGCAGAAAACTTCGATATCGAAATCTTATCATCGTCCAAAACCTTTACCATTGCAGATTTGGGTTGCTCTGTTGGTCCTAACACATTTTATTCTGTCCAAAACATACTCGAAGCCGTGGAGCACAAGTGCCAAACCCAAGGGCTGAATGCTCAAATCCCAGAATTTCAAGTCGTTTTTAATGACAATACTCCGAATGATTTTAATACGCTCTTCAAATCCCTCCCTCAGAACAGACGATACTGTGCAGTGGGCGTGCCTGGCTCTTTCTACGGTCGCCTATTTCCTAATGCTTCTGTACAAGTTGTTCACTCCTCATATGCCAATCACTGGCTTTCTAGAGTGCCAAAAGAAGTAGCGGACAGAAACAGTATGGCGTGGAACAAAGGACGAATTCATTACTCAAATTCGACCGATGAAGTACTAAAGGCTTATGAAGCTCAATACGCCGAGGACATGGAGTGCTTCTTAAATGTCAGGGCACAAGAGATTGTGTACGGAGGACTGATGGTACTTATCATCGTAGGCCGCCCTGATGGTACCCCTCATTCTCTTTCTCAGGCAAATATGACCTTCCAAATTTTAGGATCTTGCCTCGTTGACTTGGTTAGAAAGGGAATAGTTGATGAGGAGAAAGTAGACTCTTTTAACGTACCTATGTACTGCATGTCTCCCGGAGAAGTGGAAGCTGTGGTAGGACGAAATGGATGTTTTAGCATGGAGAGAATAGAGAACTTACCTGCTTTCGTGCCCCCGGACAATGTCTCTAAAGCCAAACTGCTTGCAACTCACATGAGAGCTGCAATGGAAGGAGTCATCAAACAACATTTCGGAGAAGAAATCTTAGACGAGGTCTTTGACTTATACCGCAAGAAAATTGAAGAGCAATCCTCAATCTTTGATGCAGGAAAGTCGATAAGCTTTCTCGTTGTGCTTAAACGCAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

362

Amino Acids

39.96

Weight (kDa)

5.39

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 51 - 359 5.6e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 883
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 465
AccI GTMKAC 1 cut(s) 774
AciI CCGC 3 cut(s) 494, 664, 1003
AclI AACGTT 1 cut(s) 86
AclWI GGATC 1 cut(s) 730
AcsI RAATTY 4 cut(s) 299, 528, 541, 714
AfaI GTAC 7 cut(s) 435, 558, 632, 648, 676, 789, 797
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 4 cut(s) 42, 305, 352, 1016
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 4 cut(s) 575, 824, 935, 1056
AluI AGCT 4 cut(s) 575, 824, 935, 1056
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 810, 904
AlwI GGATC 1 cut(s) 730
AoxI GGCC 1 cut(s) 661
ApeKI GCWGC 2 cut(s) 5, 935
ApoI RAATTY 4 cut(s) 299, 528, 541, 714
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
Asp718I GGTACC 1 cut(s) 674
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 810, 890
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 2 cut(s) 619, 888
BanI GGYRCC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 17, 922
BccI CCATC 4 cut(s) 50, 59, 637, 665
BceAI ACGGC 1 cut(s) 236
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 1029, 1063
BciT130I CCWGG 1 cut(s) 396
BcnI CCSGG 2 cut(s) 810, 890
BcoDI GTCTC 2 cut(s) 810, 904
BfaI CTAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 883
BisI GCNGC 3 cut(s) 6, 664, 936
BlsI GCNGC 3 cut(s) 7, 665, 937
BmcAI AGTACT 1 cut(s) 558
Bme1390I CCNGG 3 cut(s) 396, 810, 890
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 61, 676
BmrFI CCNGG 3 cut(s) 396, 810, 890
BmsI GCATC 2 cut(s) 97, 1027
BplI GAGNNNNNCTC 2 cut(s) 1010, 1042
BpuMI CCSGG 2 cut(s) 810, 890
BsaJI CCNNGG 4 cut(s) 252, 274, 585, 888
BsaWI WCCGGW 1 cut(s) 10
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse1I ACTGG 1 cut(s) 470
Bse3DI GCAATG 2 cut(s) 186, 945
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 4 cut(s) 252, 274, 585, 888
BseGI GGATG 2 cut(s) 112, 848
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMI GCAATG 2 cut(s) 186, 945
BseMII CTCAG 3 cut(s) 42, 377, 707
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 3 cut(s) 111, 439, 779
BseSI GKGCMC 2 cut(s) 619, 888
BseXI GCAGC 2 cut(s) 17, 922
BsgI GTGCAG 1 cut(s) 402
Bsh1285I CGRYCG 2 cut(s) 412, 549
BshFI GGCC 1 cut(s) 663
BshNI GGYRCC 1 cut(s) 674
BsiEI CGRYCG 2 cut(s) 412, 549
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 11, 810, 890
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 2 cut(s) 810, 904
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 663
Bsp1286I GDGCHC 3 cut(s) 261, 619, 888
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 1 cut(s) 722
BspACI CCGC 3 cut(s) 494, 664, 1003
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 3 cut(s) 41, 376, 706
BspLI GGNNCC 2 cut(s) 61, 676
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 2 cut(s) 353, 1011
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 186, 945
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 252, 274, 585, 888
BssMI GATC 1 cut(s) 722
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 3 cut(s) 380, 410, 506
Bst6I CTCTTC 2 cut(s) 353, 1011
BstAUI TGTACA 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 19, 392, 918
BstDEI CTNAG 6 cut(s) 28, 125, 363, 693, 979, 1083
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 112, 848
BstKTI GATC 1 cut(s) 725
BstMAI GTCTC 2 cut(s) 810, 904
BstMBI GATC 1 cut(s) 722
BstMCI CGRYCG 2 cut(s) 412, 549
BstMWI GCNNNNNNNGC 5 cut(s) 14, 66, 396, 572, 581
BstNI CCWGG 1 cut(s) 396
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 3 cut(s) 394, 808, 888
BstSLI GKGCMC 2 cut(s) 619, 888
BstV1I GCAGC 2 cut(s) 17, 922
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 1 cut(s) 663
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 112, 848
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 2 cut(s) 390, 463
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 3 cut(s) 19, 392, 918
Cfr13I GGNCC 1 cut(s) 212
Csp6I GTAC 7 cut(s) 434, 557, 631, 647, 675, 788, 796
CviAII CATG 4 cut(s) 592, 802, 853, 928
CviQI GTAC 7 cut(s) 434, 557, 631, 647, 675, 788, 796
DdeI CTNAG 6 cut(s) 28, 125, 363, 693, 979, 1083
DpnI GATC 1 cut(s) 724
DpnII GATC 1 cut(s) 722
Eam1104I CTCTTC 2 cut(s) 353, 1011
EarI CTCTTC 2 cut(s) 353, 1011
Eco130I CCWWGG 1 cut(s) 274
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 212
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 394
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 4 cut(s) 595, 805, 856, 931
FatI CATG 4 cut(s) 591, 801, 852, 927
FauNDI CATATG 1 cut(s) 454
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 3 cut(s) 6, 664, 936
FokI GGATG 2 cut(s) 119, 855
Fsp4HI GCNGC 3 cut(s) 6, 664, 936
FspBI CTAG 1 cut(s) 474
GluI GCNGC 3 cut(s) 6, 664, 936
HaeIII GGCC 1 cut(s) 663
HapII CCGG 3 cut(s) 11, 810, 890
Hin1II CATG 4 cut(s) 595, 805, 856, 931
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 1 cut(s) 1054
HinfI GANTC 2 cut(s) 776, 948
HpaII CCGG 3 cut(s) 11, 810, 890
Hpy166II GTNNAC 4 cut(s) 445, 631, 736, 775
Hpy188I TCNGA 5 cut(s) 31, 64, 330, 366, 968
Hpy188III TCNNGA 2 cut(s) 91, 474
Hpy8I GTNNAC 4 cut(s) 445, 631, 736, 775
HpyAV CCTTC 2 cut(s) 718, 938
HpyCH4III ACNGT 3 cut(s) 380, 410, 506
HpyCH4IV ACGT 2 cut(s) 86, 786
HpyCH4V TGCA 7 cut(s) 140, 191, 383, 801, 920, 938, 1040
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 66, 396, 572, 581
HpyF3I CTNAG 6 cut(s) 28, 125, 363, 693, 979, 1083
HpySE526I ACGT 2 cut(s) 86, 786
Hsp92II CATG 4 cut(s) 595, 805, 856, 931
KpnI GGTACC 1 cut(s) 678
Kzo9I GATC 1 cut(s) 722
LguI GCTCTTC 2 cut(s) 353, 1011
LmnI GCTCC 2 cut(s) 65, 256
Lsp1109I GCAGC 2 cut(s) 17, 922
LweI GCATC 2 cut(s) 97, 1027
MaeI CTAG 1 cut(s) 474
MaeII ACGT 2 cut(s) 86, 786
MalI GATC 1 cut(s) 724
MboI GATC 1 cut(s) 722
MboII GAAGA 3 cut(s) 340, 983, 1028
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 3 cut(s) 261, 619, 888
MluCI AATT 6 cut(s) 135, 299, 528, 541, 714, 1011
MlyI GAGTC 2 cut(s) 770, 957
MseI TTAA 5 cut(s) 315, 339, 605, 783, 1071
MspI CCGG 3 cut(s) 11, 810, 890
MspR9I CCNGG 3 cut(s) 396, 810, 890
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 5 cut(s) 14, 66, 396, 572, 581
NciI CCSGG 2 cut(s) 810, 890
NdeI CATATG 1 cut(s) 454
NdeII GATC 1 cut(s) 722
NlaIII CATG 4 cut(s) 595, 805, 856, 931
NlaIV GGNNCC 2 cut(s) 61, 676
NmeAIII GCCGAG 1 cut(s) 610
NspI RCATGY 1 cut(s) 805
PciSI GCTCTTC 2 cut(s) 353, 1011
PctI GAATGC 1 cut(s) 289
PflFI GACNNNGTC 2 cut(s) 896, 986
PflMI CCANNNNNTGG 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 3 cut(s) 7, 665, 937
PleI GAGTC 2 cut(s) 770, 956
PpsI GAGTC 2 cut(s) 770, 956
Psp1406I AACGTT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 61, 676
PspPI GGNCC 1 cut(s) 212
PsrI GAACNNNNNNTAC 4 cut(s) 359, 391, 426, 458
PsuI RGATCY 1 cut(s) 722
PsyI GACNNNGTC 2 cut(s) 896, 986
RsaI GTAC 7 cut(s) 435, 558, 632, 648, 676, 789, 797
RsaNI GTAC 7 cut(s) 434, 557, 631, 647, 675, 788, 796
SapI GCTCTTC 2 cut(s) 353, 1011
SaqAI TTAA 5 cut(s) 315, 339, 605, 783, 1071
SatI GCNGC 3 cut(s) 6, 664, 936
Sau3AI GATC 1 cut(s) 722
Sau96I GGNCC 1 cut(s) 212
ScaI AGTACT 1 cut(s) 558
SchI GAGTC 2 cut(s) 770, 957
ScrFI CCNGG 3 cut(s) 396, 810, 890
SduI GDGCHC 3 cut(s) 261, 619, 888
SfaNI GCATC 2 cut(s) 97, 1027
SinI GGWCC 1 cut(s) 212
Sse9I AATT 6 cut(s) 135, 299, 528, 541, 714, 1011
SsiI CCGC 3 cut(s) 494, 664, 1003
SspMI CTAG 1 cut(s) 474
StyD4I CCNGG 3 cut(s) 394, 808, 888
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 3 cut(s) 380, 410, 506
TaiI ACGT 2 cut(s) 89, 789
TaqI TCGA 5 cut(s) 150, 156, 246, 545, 1049
TaqII GACCGA 1 cut(s) 563
TasI AATT 6 cut(s) 135, 299, 528, 541, 714, 1011
TatI WGTACW 3 cut(s) 433, 556, 795
TauI GCSGC 1 cut(s) 666
Tru1I TTAA 5 cut(s) 315, 339, 605, 783, 1071
Tru9I TTAA 5 cut(s) 315, 339, 605, 783, 1071
TscAI CASTG 2 cut(s) 390, 470
TseI GCWGC 2 cut(s) 5, 935
TspDTI ATGAA 4 cut(s) 59, 521, 567, 585
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 2 cut(s) 390, 470
Tth111I GACNNNGTC 2 cut(s) 896, 986
Van91I CCANNNNNTGG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 4 cut(s) 299, 528, 541, 714
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 1 cut(s) 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 1 cut(s) 474
ZrmI AGTACT 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.