MD09G1254000.v1.1

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000151
Physical Location & Seq
Forward (+)
32546579 .. 32548263
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1254000.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGCAGCTGCAATGGGGGATTCCATTAATGTTTCTGAGGCATATCCAATGAAAGGTGGAGATGGCCCCGACAGCTATGCCAAGAACTCTATCGAACAGAAAGTAGTTGTTGATGCTACCAAAGAACTTCTAAGCAAGGCTATTAAAGAAAAGCTTGAACTGGACATGTTTTTACCATTCAACACCTTCCATATTGCAGATTTGGGTTGTTCGGTTGGGCCTAATACATTTTTTTCTGTTGAAAATATACTTGAAGCTGTGAAATTTGAGTATCAAAGCGGAGGGCCGAGTTCTGAAGTCCCCAAATTTCAAGTTTTCTTCAGTGATAAAACCCTAAATGATTTTAACATACTCTTCAATTACCTCCCTCAGAACAGGAACTATTATGCAGCGGGTGTGCCAGGTTCTTTCCACGGTCGCTTGTTTCCCAAGGCTTCCATTAACTTTTTTCACTCTTCATTAGCCCTTCATTGGCTTTCTAGGGTACCAAAAGATGTAATGGACAAAAACAGTCGTGCTTGGAATAAAGGACGAATCCACTACTCAAATAACCAAGACGAAGTATTGAAGGCATATCAATCTCAATATGCTGAGGACATGGATTGTTTCCTCCGTGCCAGAGCACTTGAGACTGTGCATCGAGGATTGATTGTGCTTAACCTACTAGGACGCCCTCTTAACTGTATTGAAATTGTGGGTTTTGACATTTTTGGATCTTGCCTCGTGGAGATGGCTACGAAGGGGGTAATTAGTGAAGAAAAAGTAGATTCATTTAACATCCCTTCCTACTTCGTGTCTCCCCAAGAACTGGAAGTCATTGTGGAACGAAATGGATGCTTTAGCATAGAGATATTGGAAACCTTACCTCAGCATGTCTTGAGTGGCTCTCCCAGTGCTCCTGCTATGAGAGCTTTCACTGAGGGACTGATCAAGCAACAATTTGGAGAAGAAATCTTAGATGATCTCTTCAGCTTGTATCACCAGAAACTTGAAGAGCAAATGTCGATAGCTGAGTCAAAGAAGGCAGTTGTCCTTTTTGTTGTGCTTAAACGCAAGACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

355

Amino Acids

39.72

Weight (kDa)

5.48

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 52 - 351 2.6e-97 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 484
AccB7I CCANNNNNTGG 1 cut(s) 808
AciI CCGC 2 cut(s) 279, 392
AclWI GGATC 1 cut(s) 721
AcsI RAATTY 2 cut(s) 263, 305
AcuI CTGAAG 3 cut(s) 304, 315, 952
AcyI GRCGYC 1 cut(s) 670
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 53, 471, 808
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 9 cut(s) 158, 181, 242, 254, 311, 358, 568, 689, 992
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 7 cut(s) 8, 75, 154, 257, 911, 972, 1010
AluI AGCT 7 cut(s) 8, 75, 154, 257, 911, 972, 1010
Alw21I GWGCWC 2 cut(s) 625, 898
Alw26I GTCTC 2 cut(s) 623, 801
AlwI GGATC 1 cut(s) 721
AoxI GGCC 3 cut(s) 64, 218, 284
ApeKI GCWGC 3 cut(s) 5, 8, 389
ApoI RAATTY 2 cut(s) 263, 305
AseI ATTAAT 1 cut(s) 27
Asp718I GGTACC 1 cut(s) 484
AspS9I GGNCC 3 cut(s) 65, 218, 284
AsuHPI GGTGA 1 cut(s) 971
BanI GGYRCC 1 cut(s) 484
BauI CACGAG 1 cut(s) 722
Bbv12I GWGCWC 2 cut(s) 625, 898
BbvCI CCTCAGC 2 cut(s) 591, 867
BbvI GCAGC 2 cut(s) 17, 401
BccI CCATC 2 cut(s) 56, 724
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 816, 850
BciT130I CCWGG 1 cut(s) 402
BclI TGATCA 1 cut(s) 927
BcoDI GTCTC 2 cut(s) 623, 801
BfaI CTAG 2 cut(s) 480, 665
BisI GCNGC 3 cut(s) 6, 9, 390
BlsI GCNGC 3 cut(s) 7, 10, 391
Bme1390I CCNGG 1 cut(s) 402
BmgT120I GGNCC 3 cut(s) 65, 218, 284
BmiI GGNNCC 2 cut(s) 67, 486
BmrFI CCNGG 1 cut(s) 402
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 3 cut(s) 103, 646, 824
BmuI ACTGGG 1 cut(s) 885
BplI GAGNNNNNCTC 2 cut(s) 871, 903
Bpu10I CCTNAGC 2 cut(s) 591, 867
BpuEI CTTGAG 2 cut(s) 647, 898
BsaHI GRCGYC 1 cut(s) 670
BsaJI CCNNGG 2 cut(s) 412, 429
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 471, 808
Bse1I ACTGG 3 cut(s) 165, 813, 891
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 412, 429
BseGI GGATG 2 cut(s) 777, 839
BseLI CCNNNNNNNGG 3 cut(s) 53, 471, 808
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 6 cut(s) 27, 383, 582, 881, 909, 1002
BseNI ACTGG 3 cut(s) 165, 813, 891
BseXI GCAGC 2 cut(s) 17, 401
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 3 cut(s) 66, 220, 286
BshNI GGYRCC 1 cut(s) 484
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 625, 898
BslFI GGGAC 2 cut(s) 284, 936
BslI CCNNNNNNNGG 3 cut(s) 53, 471, 808
BsmAI GTCTC 2 cut(s) 623, 801
BsmFI GGGAC 2 cut(s) 284, 936
BsnI GGCC 3 cut(s) 66, 220, 286
Bsp1286I GDGCHC 2 cut(s) 625, 898
Bsp143I GATC 3 cut(s) 713, 927, 961
BspACI CCGC 2 cut(s) 279, 392
BspANI GGCC 3 cut(s) 66, 220, 286
BspCNI CTCAG 6 cut(s) 28, 382, 583, 880, 910, 1003
BspLI GGNNCC 2 cut(s) 67, 486
BspPI GGATC 1 cut(s) 721
BspQI GCTCTTC 1 cut(s) 987
BspT107I GGYRCC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 3 cut(s) 165, 813, 891
BssECI CCNNGG 2 cut(s) 412, 429
BssMI GATC 3 cut(s) 713, 927, 961
BssNI GRCGYC 1 cut(s) 670
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 416, 512, 634, 683
Bst6I CTCTTC 4 cut(s) 359, 460, 971, 987
BstACI GRCGYC 1 cut(s) 670
BstDEI CTNAG 8 cut(s) 36, 131, 369, 591, 867, 918, 955, 1011
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 2 cut(s) 777, 839
BstKTI GATC 3 cut(s) 716, 930, 964
BstMAI GTCTC 2 cut(s) 623, 801
BstMBI GATC 3 cut(s) 713, 927, 961
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 2 cut(s) 72, 908
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 2 cut(s) 169, 875
BstSCI CCNGG 1 cut(s) 400
BstV1I GCAGC 2 cut(s) 17, 401
BstX2I RGATCY 1 cut(s) 713
BstYI RGATCY 1 cut(s) 713
BsuRI GGCC 3 cut(s) 66, 220, 286
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 2 cut(s) 777, 839
BtsIMutI CAGTG 3 cut(s) 328, 898, 915
Cfr13I GGNCC 3 cut(s) 65, 218, 284
CseI GACGC 1 cut(s) 678
Csp6I GTAC 1 cut(s) 485
CviAII CATG 3 cut(s) 166, 598, 872
CviQI GTAC 1 cut(s) 485
DdeI CTNAG 8 cut(s) 36, 131, 369, 591, 867, 918, 955, 1011
DpnI GATC 3 cut(s) 715, 929, 963
DpnII GATC 3 cut(s) 713, 927, 961
Eam1104I CTCTTC 4 cut(s) 359, 460, 971, 987
EarI CTCTTC 4 cut(s) 359, 460, 971, 987
Eco130I CCWWGG 1 cut(s) 429
Eco57I CTGAAG 3 cut(s) 304, 315, 952
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 3 cut(s) 169, 601, 875
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 2 cut(s) 284, 936
FatI CATG 3 cut(s) 165, 597, 871
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 1 cut(s) 927
Fnu4HI GCNGC 3 cut(s) 6, 9, 390
FokI GGATG 2 cut(s) 764, 846
Fsp4HI GCNGC 3 cut(s) 6, 9, 390
FspBI CTAG 2 cut(s) 480, 665
GluI GCNGC 3 cut(s) 6, 9, 390
HaeIII GGCC 3 cut(s) 66, 220, 286
HgaI GACGC 1 cut(s) 678
Hin1I GRCGYC 1 cut(s) 670
Hin1II CATG 3 cut(s) 169, 601, 875
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 4 cut(s) 20, 534, 767, 1013
HphI GGTGA 1 cut(s) 971
Hpy188I TCNGA 3 cut(s) 37, 295, 372
Hpy188III TCNNGA 1 cut(s) 877
HpyAV CCTTC 6 cut(s) 196, 476, 562, 733, 792, 1015
HpyCH4III ACNGT 4 cut(s) 416, 512, 634, 683
HpyCH4V TGCA 4 cut(s) 11, 197, 389, 637
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 908
HpyF3I CTNAG 8 cut(s) 36, 131, 369, 591, 867, 918, 955, 1011
Hsp92I GRCGYC 1 cut(s) 670
Hsp92II CATG 3 cut(s) 169, 601, 875
KpnI GGTACC 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 927
Kzo9I GATC 3 cut(s) 713, 927, 961
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 1 cut(s) 901
LpnPI CCDG 9 cut(s) 146, 361, 387, 414, 631, 794, 904, 912, 995
Lsp1109I GCAGC 2 cut(s) 17, 401
LweI GCATC 3 cut(s) 103, 646, 824
MaeI CTAG 2 cut(s) 480, 665
MalI GATC 3 cut(s) 715, 929, 963
MboI GATC 3 cut(s) 713, 927, 961
MboII GAAGA 7 cut(s) 310, 346, 447, 767, 958, 959, 1004
MflI RGATCY 1 cut(s) 713
MhlI GDGCHC 2 cut(s) 625, 898
MluCI AATT 7 cut(s) 263, 305, 358, 690, 747, 938, 1060
MlyI GAGTC 1 cut(s) 1022
MseI TTAA 8 cut(s) 27, 144, 345, 441, 657, 678, 774, 1047
MspA1I CMGCKG 2 cut(s) 8, 392
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 2 cut(s) 72, 908
NdeII GATC 3 cut(s) 713, 927, 961
NlaIII CATG 3 cut(s) 169, 601, 875
NlaIV GGNNCC 2 cut(s) 67, 486
NmeAIII GCCGAG 1 cut(s) 312
NspI RCATGY 2 cut(s) 169, 875
PciI ACATGT 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 20, 534, 767
PflMI CCANNNNNTGG 1 cut(s) 808
PkrI GCNGC 3 cut(s) 7, 10, 391
PleI GAGTC 1 cut(s) 1021
PpsI GAGTC 1 cut(s) 1021
PscI ACATGT 1 cut(s) 165
PshBI ATTAAT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 67, 486
PspPI GGNCC 3 cut(s) 65, 218, 284
PsuI RGATCY 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 8 cut(s) 27, 144, 345, 441, 657, 678, 774, 1047
SatI GCNGC 3 cut(s) 6, 9, 390
Sau3AI GATC 3 cut(s) 713, 927, 961
Sau96I GGNCC 3 cut(s) 65, 218, 284
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 2 cut(s) 625, 898
SfaNI GCATC 3 cut(s) 103, 646, 824
SmlI CTYRAG 2 cut(s) 626, 877
SmoI CTYRAG 2 cut(s) 626, 877
Sse9I AATT 7 cut(s) 263, 305, 358, 690, 747, 938, 1060
SsiI CCGC 2 cut(s) 279, 392
SspMI CTAG 2 cut(s) 480, 665
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 4 cut(s) 416, 512, 634, 683
TaqI TCGA 3 cut(s) 93, 640, 1004
TasI AATT 7 cut(s) 263, 305, 358, 690, 747, 938, 1060
TfiI GAWTC 3 cut(s) 20, 534, 767
Tru1I TTAA 8 cut(s) 27, 144, 345, 441, 657, 678, 774, 1047
Tru9I TTAA 8 cut(s) 27, 144, 345, 441, 657, 678, 774, 1047
TscAI CASTG 3 cut(s) 328, 898, 922
TseI GCWGC 3 cut(s) 5, 8, 389
TspDTI ATGAA 4 cut(s) 65, 447, 458, 759
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 3 cut(s) 328, 898, 922
Van91I CCANNNNNTGG 1 cut(s) 808
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 2 cut(s) 263, 305
XceI RCATGY 2 cut(s) 169, 875
XspI CTAG 2 cut(s) 480, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.