Rw7G001650

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000151
Physical Location & Seq
Forward (+)
1385152 .. 1390629
5478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001650.1

Sequence Viewer

Length: 2163 bp
ATGGCAGCAGATGATACCAGTAATGTATTTGAAGCACATCCAATGGAAGGTGGAAATGGCCCCAACAGCTATGCCAAGAACTCCATTGTGCAGAGAGGAGTTGTGGATGCTGCCAAAGAACTTGTAAAAAGGGCAATTGTAGAAACACTCGACATAGACATCTTGTCATCTTCAAACTCCTTTAAAATTGCAGATCTGGGTTGCTCTGTTGGGCCTAATACATTTTATGCAGTTGAAAACATGATTGAAGCTGTGGAGTTCAAGTATCAAAGCCAAGGGCAGAATTCACAAATCCCAGAATTTCAAGTCTTGTTTAATGACCATGCCTCAAATGACTTTAACATGCTCTTTATATCCTTCCCTGAGAACAAACGATACTATGCAGCTGGTGTGCCTGGTTCTTTCTATAGTAGAGTATTTCCTAATGCTTCCATCCACTTAGTTCACTCTTCCTATGCCATTCATTGGCTTTCTAGAGTACCGAAACAGGTGTTGGACAAACACAATGAAGTAGTAAGGGCTTATGAAGCTCAATATGTCGAGGACATGGAGTGCTTCCTGCATGCTAGAGCACAAGAGATTGTGTATGGAGGACTAATGGTACTTATCTTTCCAGGCCGCCCCAATGGCTCCGCTAGTTCTCAAGCTTGGGCAAATATGAGCTTCCAAGTTTTGGGATCTTGCCTCATGGACTTGGTTAGAAAGAAAGTGGATTCATTCAACATGCCTATATATTCCATGACTCCTCAAGAACTTGAAGATGCTGTGAAACAGAATGGATGCTTTAGCGTAGAGATAATGGCAAACTTACCTCATCCCTTGGTAGATGACACTCTTTCAGTACCGCAAGTACTTGCCTCTCACCTGAGAACTGGCGTGGAGGGGATGGTCAAGAAGCAATTTGGAGAAGAAATATTAGATGAGCTCTTCGACTTGTATCGGGAAAAATGTGAGCATGATGCGCTCAACTTTCTTGCTGTGCTTGGACACAACTTTATTGTTGTTCTTAGACTAGTAGATATAAATTATGACGACCCCTACAATACAGGATGCAATACACAGAGAAACAAAGAGATGGCAGCAGAGAATACCAGTAAAGTCTTTGAAGCACATCCTATGAAAGGTGGAGATGGCCCCAATAGCTATACAAAAAATTCCATTCTGCAGAGAGCTTCTGTCGATGCTGCCAAAGAACTTCTGAACAAGGCAATTGCAGAAAAGCTGGACGTCAAAAGTTTCTTACCTTCCAAGTCTTTTCGCATTGCAGATCTGGGTTGCTCTACTGGACCAAATACTTTTATGGCAGTTGGAAACATACTTGAAGCTGTGGAGTCCAAGTATCGAAGCCAAGGGCTGAATTCTCAGATTCCCGAATTTCAAGTGTTCTTTAATGATCATGCCTCAAATGACTTTAACATGCTCTTCCAGTCCCTCCCTCAGAACAGGCAATACCATGCAGCCGGTGTACCTGGTTCTTTCTACAAACAGGTGTTACCTAATGCTTCCATTAACTTTGCTTACTCTTCTACTGCCATTCAATGGCTCTCTAGAGTACCAACAGCAGTAGCAGATAGTAATAGTCCTGCGTGGAACAAAGGACACATTCATTACTCAAATGCCACAGATGAAGTAATAAGGGCTTATGAAACTCAATACAGTGATGACATGGAGAGCTTCCTGCAAGCCAGGGCACAAGAGATTGTGTACGGAGGATTAATTGTACTTACATTTCCAGGCCGCCACAGTGACACCCCTCATTCTGATGCTCTGCCAAACATGGTCTTACAGCTTTTAGGAGCCTCCCTCATGGACTTGGTTAGAAAGGGAGTAGTTAGCGAAGAGAAGATAGATTCATTTAACATACCTGTGTATTCAATGTCTCCCAAAGAACTAGCAGCTGCTGTAGAAAGAAATGGATGTTTTAGCATAGAGATGGTGGCAGACTTGCTTGTTCCCCCGGTAGATGACACTAGCTCAATGCCACAACTAATTGCCTCTCACATTAGAGCTGGCATGGAGGGGATATTGAAGCAGCAATTTGGAGAGGAACTTATAGATGAGCTCTTCGATTTGTATCGGGAAAAATGTGAAGAGCATATCTCCACCACAGACACAGGGAAGCCAGTTAACTTCCTTGTGGTGCTTAGACGCAAGGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

720

Amino Acids

80.13

Weight (kDa)

5.1

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 323 2.1e-85 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 408 - 717 1.4e-103 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1230
AccB7I CCANNNNNTGG 3 cut(s) 465, 673, 1539
AciI CCGC 4 cut(s) 619, 633, 845, 1738
AclWI GGATC 1 cut(s) 685
AcsI RAATTY 5 cut(s) 283, 299, 1153, 1357, 1373
AcyI GRCGYC 1 cut(s) 1227
AfaI GTAC 8 cut(s) 480, 603, 843, 852, 1467, 1554, 1706, 1722
AfiI CCNNNNNNNGG 5 cut(s) 47, 465, 673, 1121, 1539
AhdI GACNNNNNGTC 1 cut(s) 163
AhlI ACTAGT 1 cut(s) 1012
AjnI CCWGG 5 cut(s) 394, 613, 1468, 1685, 1732
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
Alw21I GWGCWC 3 cut(s) 574, 927, 2064
Alw26I GTCTC 1 cut(s) 1884
AlwI GGATC 1 cut(s) 685
AlwNI CAGNNNCTG 1 cut(s) 1901
AoxI GGCC 5 cut(s) 58, 212, 616, 1132, 1735
ApeKI GCWGC 9 cut(s) 5, 110, 383, 1079, 1184, 1457, 1895, 1898, 2032
ApoI RAATTY 5 cut(s) 283, 299, 1153, 1357, 1373
AseI ATTAAT 1 cut(s) 1715
AspLEI GCGC 1 cut(s) 964
AspS9I GGNCC 4 cut(s) 59, 212, 1133, 1286
AsuC2I CCSGG 1 cut(s) 1958
AsuHPI GGTGA 1 cut(s) 854
AvaII GGWCC 1 cut(s) 1286
BaeGI GKGCMC 1 cut(s) 1693
BanII GRGCYC 2 cut(s) 927, 2064
Bbv12I GWGCWC 3 cut(s) 574, 927, 2064
BbvI GCAGC 9 cut(s) 17, 97, 395, 1091, 1171, 1469, 1885, 1907, 2044
BccI CCATC 5 cut(s) 440, 880, 1069, 1124, 1927
BciT130I CCWGG 5 cut(s) 396, 615, 1470, 1687, 1734
BclI TGATCA 1 cut(s) 1393
BcnI CCSGG 1 cut(s) 1958
BcoDI GTCTC 1 cut(s) 1884
BcuI ACTAGT 1 cut(s) 1012
BfaI CTAG 7 cut(s) 474, 567, 636, 1013, 1548, 1892, 1971
BfmI CTRYAG 3 cut(s) 406, 1163, 1902
BglI GCCNNNNNGGC 1 cut(s) 627
BglII AGATCT 2 cut(s) 193, 1267
BmcAI AGTACT 1 cut(s) 852
Bme1390I CCNGG 6 cut(s) 396, 615, 1470, 1687, 1734, 1958
Bme18I GGWCC 1 cut(s) 1286
BmeRI GACNNNNNGTC 1 cut(s) 163
BmgT120I GGNCC 4 cut(s) 59, 212, 1133, 1286
BmiI GGNNCC 4 cut(s) 61, 631, 1135, 1798
BmrFI CCNGG 6 cut(s) 396, 615, 1470, 1687, 1734, 1958
BmsI GCATC 7 cut(s) 97, 751, 770, 949, 1040, 1171, 1753
BplI GAGNNNNNCTC 4 cut(s) 1788, 1820, 2084, 2116
BpuEI CTTGAG 2 cut(s) 627, 732
BpuMI CCSGG 1 cut(s) 1958
BsaBI GATNNNNATC 1 cut(s) 2073
BsaHI GRCGYC 1 cut(s) 1227
BsaJI CCNNGG 5 cut(s) 274, 819, 1348, 1686, 1956
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 1322, 1352
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 465, 673, 1121, 1539
Bse118I RCCGGY 1 cut(s) 1460
Bse1I ACTGG 6 cut(s) 18, 877, 1092, 1288, 1426, 2123
Bse3DI GCAATG 1 cut(s) 1260
Bse8I GATNNNNATC 1 cut(s) 2073
BseBI CCWGG 5 cut(s) 396, 615, 1470, 1687, 1734
BseDI CCNNGG 5 cut(s) 274, 819, 1348, 1686, 1956
BseGI GGATG 9 cut(s) 37, 112, 432, 785, 814, 891, 1055, 1111, 1922
BseJI GATNNNNATC 1 cut(s) 2073
BseLI CCNNNNNNNGG 5 cut(s) 47, 465, 673, 1121, 1539
BseMI GCAATG 1 cut(s) 1260
BseMII CTCAG 4 cut(s) 354, 857, 1376, 1451
BseNI ACTGG 6 cut(s) 18, 877, 1092, 1288, 1426, 2123
BseRI GAGGAG 2 cut(s) 111, 735
BseSI GKGCMC 1 cut(s) 1693
BseXI GCAGC 9 cut(s) 17, 97, 395, 1091, 1171, 1469, 1885, 1907, 2044
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 5 cut(s) 60, 214, 618, 1134, 1737
BsiHKAI GWGCWC 3 cut(s) 574, 927, 2064
BsiSI CCGG 2 cut(s) 1461, 1958
BslFI GGGAC 1 cut(s) 1414
BslI CCNNNNNNNGG 5 cut(s) 47, 465, 673, 1121, 1539
BsmAI GTCTC 1 cut(s) 1884
BsmFI GGGAC 1 cut(s) 1414
BsnI GGCC 5 cut(s) 60, 214, 618, 1134, 1737
Bsp1286I GDGCHC 4 cut(s) 574, 927, 1693, 2064
Bsp143I GATC 4 cut(s) 193, 677, 1267, 1393
BspACI CCGC 4 cut(s) 619, 633, 845, 1738
BspANI GGCC 5 cut(s) 60, 214, 618, 1134, 1737
BspCNI CTCAG 4 cut(s) 355, 858, 1375, 1450
BspLI GGNNCC 4 cut(s) 61, 631, 1135, 1798
BspMAI CTGCAG 1 cut(s) 1167
BspPI GGATC 1 cut(s) 685
BspQI GCTCTTC 4 cut(s) 932, 1427, 2069, 2085
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 1 cut(s) 1460
BsrI ACTGG 6 cut(s) 18, 877, 1092, 1288, 1426, 2123
BssAI RCCGGY 1 cut(s) 1460
BssECI CCNNGG 5 cut(s) 274, 819, 1348, 1686, 1956
BssMI GATC 4 cut(s) 193, 677, 1267, 1393
BssNI GRCGYC 1 cut(s) 1227
BssT1I CCWWGG 3 cut(s) 274, 819, 1348
Bst2UI CCWGG 5 cut(s) 396, 615, 1470, 1687, 1734
Bst4CI ACNGT 2 cut(s) 1658, 1745
Bst6I CTCTTC 7 cut(s) 454, 932, 1427, 1528, 1833, 2069, 2085
BstACI GRCGYC 1 cut(s) 1227
BstC8I GCNNGC 3 cut(s) 564, 1683, 2011
BstDEI CTNAG 7 cut(s) 363, 439, 866, 1007, 1362, 1437, 2144
BstENI CCTNNNNNAGG 1 cut(s) 1119
BstF5I GGATG 9 cut(s) 37, 112, 432, 785, 814, 891, 1055, 1111, 1922
BstHHI GCGC 1 cut(s) 964
BstKTI GATC 4 cut(s) 196, 680, 1270, 1396
BstMAI GTCTC 1 cut(s) 1884
BstMBI GATC 4 cut(s) 193, 677, 1267, 1393
BstMWI GCNNNNNNNGC 5 cut(s) 66, 527, 627, 961, 1140
BstNI CCWGG 5 cut(s) 396, 615, 1470, 1687, 1734
BstNSI RCATGY 4 cut(s) 346, 566, 727, 1420
BstSCI CCNGG 6 cut(s) 394, 613, 1468, 1685, 1732, 1956
BstSFI CTRYAG 3 cut(s) 406, 1163, 1902
BstSLI GKGCMC 1 cut(s) 1693
BstV1I GCAGC 9 cut(s) 17, 97, 395, 1091, 1171, 1469, 1885, 1907, 2044
BstX2I RGATCY 3 cut(s) 193, 677, 1267
BstYI RGATCY 3 cut(s) 193, 677, 1267
BsuRI GGCC 5 cut(s) 60, 214, 618, 1134, 1737
BtsCI GGATG 9 cut(s) 37, 112, 432, 785, 814, 891, 1055, 1111, 1922
BtsIMutI CAGTG 2 cut(s) 1663, 1750
Cac8I GCNNGC 3 cut(s) 564, 1683, 2011
CaiI CAGNNNCTG 1 cut(s) 1901
CfoI GCGC 1 cut(s) 964
Cfr10I RCCGGY 1 cut(s) 1460
Cfr13I GGNCC 4 cut(s) 59, 212, 1133, 1286
CseI GACGC 1 cut(s) 2157
CsiI ACCWGGT 1 cut(s) 1468
Csp6I GTAC 8 cut(s) 479, 602, 842, 851, 1466, 1553, 1705, 1721
CviQI GTAC 8 cut(s) 479, 602, 842, 851, 1466, 1553, 1705, 1721
DdeI CTNAG 7 cut(s) 363, 439, 866, 1007, 1362, 1437, 2144
DpnI GATC 4 cut(s) 195, 679, 1269, 1395
DpnII GATC 4 cut(s) 193, 677, 1267, 1393
DraI TTTAAA 1 cut(s) 184
DriI GACNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 7 cut(s) 454, 932, 1427, 1528, 1833, 2069, 2085
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 7 cut(s) 454, 932, 1427, 1528, 1833, 2069, 2085
Ecl136II GAGCTC 2 cut(s) 925, 2062
Eco130I CCWWGG 3 cut(s) 274, 819, 1348
Eco24I GRGCYC 2 cut(s) 927, 2064
Eco47I GGWCC 1 cut(s) 1286
Eco53kI GAGCTC 2 cut(s) 925, 2062
EcoICRI GAGCTC 2 cut(s) 925, 2062
EcoNI CCTNNNNNAGG 1 cut(s) 1119
EcoRI GAATTC 2 cut(s) 283, 1357
EcoRII CCWGG 5 cut(s) 394, 613, 1468, 1685, 1732
EcoT14I CCWWGG 3 cut(s) 274, 819, 1348
EcoT38I GRGCYC 2 cut(s) 927, 2064
ErhI CCWWGG 3 cut(s) 274, 819, 1348
FaqI GGGAC 1 cut(s) 1414
FbaI TGATCA 1 cut(s) 1393
FokI GGATG 9 cut(s) 24, 119, 419, 792, 801, 898, 1062, 1098, 1929
FriOI GRGCYC 2 cut(s) 927, 2064
FspBI CTAG 7 cut(s) 474, 567, 636, 1013, 1548, 1892, 1971
GlaI GCGC 1 cut(s) 963
HaeIII GGCC 5 cut(s) 60, 214, 618, 1134, 1737
HapII CCGG 2 cut(s) 1461, 1958
HgaI GACGC 1 cut(s) 2157
HhaI GCGC 1 cut(s) 964
Hin1I GRCGYC 1 cut(s) 1227
Hin6I GCGC 1 cut(s) 962
HinP1I GCGC 1 cut(s) 962
HincII GTYRAC 1 cut(s) 2128
HindII GTYRAC 1 cut(s) 2128
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 5 cut(s) 713, 742, 1331, 1366, 1850
HpaI GTTAAC 1 cut(s) 2128
HpaII CCGG 2 cut(s) 1461, 1958
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 4 cut(s) 445, 1466, 1705, 2128
Hpy188I TCNGA 4 cut(s) 1200, 1365, 1440, 1762
Hpy188III TCNNGA 7 cut(s) 474, 749, 892, 941, 1370, 1548, 2078
Hpy8I GTNNAC 4 cut(s) 445, 1466, 1705, 2128
HpyAV CCTTC 3 cut(s) 41, 367, 1254
HpyCH4III ACNGT 2 cut(s) 1658, 1745
HpyCH4IV ACGT 1 cut(s) 1227
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 527, 627, 961, 1140
HpyF3I CTNAG 7 cut(s) 363, 439, 866, 1007, 1362, 1437, 2144
HpySE526I ACGT 1 cut(s) 1227
Hsp92I GRCGYC 1 cut(s) 1227
HspAI GCGC 1 cut(s) 962
Ksp22I TGATCA 1 cut(s) 1393
KspAI GTTAAC 1 cut(s) 2128
Kzo9I GATC 4 cut(s) 193, 677, 1267, 1393
LguI GCTCTTC 4 cut(s) 932, 1427, 2069, 2085
LmnI GCTCC 2 cut(s) 635, 1796
Lsp1109I GCAGC 9 cut(s) 17, 97, 395, 1091, 1171, 1469, 1885, 1907, 2044
LweI GCATC 7 cut(s) 97, 751, 770, 949, 1040, 1171, 1753
MabI ACCWGGT 1 cut(s) 1468
MaeI CTAG 7 cut(s) 474, 567, 636, 1013, 1548, 1892, 1971
MaeII ACGT 1 cut(s) 1227
MaeIII GTNAC 2 cut(s) 1491, 1745
MalI GATC 4 cut(s) 195, 679, 1269, 1395
MboI GATC 4 cut(s) 193, 677, 1267, 1393
MfeI CAATTG 2 cut(s) 135, 1209
MflI RGATCY 3 cut(s) 193, 677, 1267
MhlI GDGCHC 4 cut(s) 574, 927, 1693, 2064
MlyI GAGTC 2 cut(s) 736, 1340
MmeI TCCRAC 2 cut(s) 474, 1288
MseI TTAA 9 cut(s) 183, 315, 339, 1389, 1413, 1509, 1715, 1857, 2127
MslI CAYNNNNRTG 3 cut(s) 1761, 1865, 1931
MspA1I CMGCKG 2 cut(s) 386, 1898
MspI CCGG 2 cut(s) 1461, 1958
MspR9I CCNGG 6 cut(s) 396, 615, 1470, 1687, 1734, 1958
MunI CAATTG 2 cut(s) 135, 1209
MvaI CCWGG 5 cut(s) 396, 615, 1470, 1687, 1734
MwoI GCNNNNNNNGC 5 cut(s) 66, 527, 627, 961, 1140
NciI CCSGG 1 cut(s) 1958
NdeII GATC 4 cut(s) 193, 677, 1267, 1393
NlaIV GGNNCC 4 cut(s) 61, 631, 1135, 1798
NmuCI GTSAC 1 cut(s) 1745
NspI RCATGY 4 cut(s) 346, 566, 727, 1420
PaeI GCATGC 1 cut(s) 566
PciSI GCTCTTC 4 cut(s) 932, 1427, 2069, 2085
PfeI GAWTC 3 cut(s) 713, 1366, 1850
PflMI CCANNNNNTGG 3 cut(s) 465, 673, 1539
PleI GAGTC 2 cut(s) 736, 1339
PpsI GAGTC 2 cut(s) 736, 1339
PshBI ATTAAT 1 cut(s) 1715
Psp124BI GAGCTC 2 cut(s) 927, 2064
Psp6I CCWGG 5 cut(s) 394, 613, 1468, 1685, 1732
PspGI CCWGG 5 cut(s) 394, 613, 1468, 1685, 1732
PspN4I GGNNCC 4 cut(s) 61, 631, 1135, 1798
PspPI GGNCC 4 cut(s) 59, 212, 1133, 1286
PsrI GAACNNNNNNTAC 4 cut(s) 359, 391, 1433, 1465
PstI CTGCAG 1 cut(s) 1167
PstNI CAGNNNCTG 1 cut(s) 1901
PsuI RGATCY 3 cut(s) 193, 677, 1267
PvuII CAGCTG 2 cut(s) 386, 1898
RsaI GTAC 8 cut(s) 480, 603, 843, 852, 1467, 1554, 1706, 1722
RsaNI GTAC 8 cut(s) 479, 602, 842, 851, 1466, 1553, 1705, 1721
RseI CAYNNNNRTG 3 cut(s) 1761, 1865, 1931
SacI GAGCTC 2 cut(s) 927, 2064
SapI GCTCTTC 4 cut(s) 932, 1427, 2069, 2085
SaqAI TTAA 9 cut(s) 183, 315, 339, 1389, 1413, 1509, 1715, 1857, 2127
Sau3AI GATC 4 cut(s) 193, 677, 1267, 1393
Sau96I GGNCC 4 cut(s) 59, 212, 1133, 1286
ScaI AGTACT 1 cut(s) 852
SchI GAGTC 2 cut(s) 736, 1340
ScrFI CCNGG 6 cut(s) 396, 615, 1470, 1687, 1734, 1958
SduI GDGCHC 4 cut(s) 574, 927, 1693, 2064
SexAI ACCWGGT 1 cut(s) 1468
SfaNI GCATC 7 cut(s) 97, 751, 770, 949, 1040, 1171, 1753
SfcI CTRYAG 3 cut(s) 406, 1163, 1902
SinI GGWCC 1 cut(s) 1286
SmiMI CAYNNNNRTG 3 cut(s) 1761, 1865, 1931
SmlI CTYRAG 2 cut(s) 642, 747
SmoI CTYRAG 2 cut(s) 642, 747
SpeI ACTAGT 1 cut(s) 1012
SphI GCATGC 1 cut(s) 566
SsiI CCGC 4 cut(s) 619, 633, 845, 1738
SspI AATATT 1 cut(s) 915
SspMI CTAG 7 cut(s) 474, 567, 636, 1013, 1548, 1892, 1971
SstI GAGCTC 2 cut(s) 927, 2064
StyD4I CCNGG 6 cut(s) 394, 613, 1468, 1685, 1732, 1956
StyI CCWWGG 3 cut(s) 274, 819, 1348
TaaI ACNGT 2 cut(s) 1658, 1745
TaiI ACGT 1 cut(s) 1230
TaqI TCGA 6 cut(s) 150, 540, 930, 1179, 1342, 2067
TatI WGTACW 2 cut(s) 850, 1720
TauI GCSGC 2 cut(s) 621, 1740
TfiI GAWTC 3 cut(s) 713, 1366, 1850
Tru1I TTAA 9 cut(s) 183, 315, 339, 1389, 1413, 1509, 1715, 1857, 2127
Tru9I TTAA 9 cut(s) 183, 315, 339, 1389, 1413, 1509, 1715, 1857, 2127
TscAI CASTG 2 cut(s) 1663, 1750
TseFI GTSAC 1 cut(s) 1745
TseI GCWGC 9 cut(s) 5, 110, 383, 1079, 1184, 1457, 1895, 1898, 2032
Tsp45I GTSAC 1 cut(s) 1745
TspDTI ATGAA 9 cut(s) 452, 522, 540, 705, 1133, 1595, 1641, 1659, 1842
TspGWI ACGGA 1 cut(s) 1722
TspRI CASTG 2 cut(s) 1663, 1750
Van91I CCANNNNNTGG 3 cut(s) 465, 673, 1539
VpaK11BI GGWCC 1 cut(s) 1286
VspI ATTAAT 1 cut(s) 1715
XagI CCTNNNNNAGG 1 cut(s) 1119
XapI RAATTY 5 cut(s) 283, 299, 1153, 1357, 1373
XbaI TCTAGA 2 cut(s) 473, 1547
XceI RCATGY 4 cut(s) 346, 566, 727, 1420
XspI CTAG 7 cut(s) 474, 567, 636, 1013, 1548, 1892, 1971
ZraI GACGTC 1 cut(s) 1228
ZrmI AGTACT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.