MD09G1253900.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000151
Physical Location & Seq
Forward (+)
32503485 .. 32510196
6712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1253900.v1.1.491

Sequence Viewer

Length: 891 bp
ATGAAAACTCTTTTTTTTTTCCTTTTTTTTTTCTTTCTTTCTTTTTCTTCAGGCTACTTCGAAATTCTTTTGATGTCTTCTTCATTAGTAAACTTGGAAAAGAGAAAAACTTCTTCTTTCTTAATCCTTTATGTTGGAACATCCATTGTCCCATATCTGCCAAAAAGGAAGAAGGAAAGCGGTTTAAATAGAAATACATGGGTGGGTCCCGTTTGGCCATGTGCGATGGCTATGAAAGAAAAACTTGACCTAGACATGTTTTTACCATTCAACGCCTTCCATATTGCAGATTTGGGTCGTTCAGTTGGGCCGAATACATTTTTTTCTGTTGAAAATATACTTGAAGCTGTGAAATTTGAATATCAAAGTGGAAGGCTGGGTTCTCAAATCCCGGAGTTTCAAGTTTTCTTCAGCGATCATACCTTAAATGATTTTAACATGCTCTTCAAATCCCTCCCTCAGAACAGGAACTACTATGCAGTAGGGGCCGGCCCTGAGAAAATGGGGGCCCTAGGCAAGCCTTCGAGTGGGGGTCCTAAACGAGTAATTAGTGAAGAAAAAGTAGATTCATTTAAGATCCCTTGCTACTTTGTGTCTCCCCAAGAACTGGAAGTCATTGTGGAACGAAATGGATGCTTTAGGATAGAGAGATTGGAAACCTTACCTCAGCATGTCTTGAAACATGGCACTCCCGGTGCTCCTGCTATAGCTAAAGTTACCACATCTACCTTGAGAGCTGCCACAGAGGGATTCATCAAGCAACAATTTGGTGAAGAAATTTTAGATGATCTCTTCAGCTTGTATCAGCAGAAACTTGAAGATCAAATCTCAATATATGAGTCAACGAAGCCACTTTTCCTTTTTGTTGTGCTTAAACGCAAGACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

297

Amino Acids

33.69

Weight (kDa)

8.8

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 87 - 158 3.2e-20 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 182 - 293 2.1e-22 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 607
AciI CCGC 1 cut(s) 180
AclWI GGATC 1 cut(s) 571
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 3 cut(s) 63, 353, 777
AcuI CTGAAG 3 cut(s) 33, 394, 778
AfiI CCNNNNNNNGG 2 cut(s) 527, 607
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 8 cut(s) 271, 332, 344, 359, 401, 448, 679, 818
AluBI AGCT 4 cut(s) 347, 710, 737, 798
AluI AGCT 4 cut(s) 347, 710, 737, 798
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 1 cut(s) 571
AoxI GGCC 5 cut(s) 215, 308, 486, 490, 507
ApaI GGGCCC 1 cut(s) 511
ApeKI GCWGC 1 cut(s) 737
ApoI RAATTY 3 cut(s) 63, 353, 777
Asp700I GAANNNNTTC 1 cut(s) 109
AspA2I CCTAGG 1 cut(s) 511
AspS9I GGNCC 7 cut(s) 206, 308, 486, 491, 507, 508, 533
AsuC2I CCSGG 2 cut(s) 392, 693
AsuHPI GGTGA 1 cut(s) 782
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 2 cut(s) 206, 533
AvrII CCTAGG 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 511
BalI TGGCCA 1 cut(s) 217
BanII GRGCYC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 700
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 1 cut(s) 724
BccI CCATC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 615, 649
BcnI CCSGG 2 cut(s) 392, 693
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 2 cut(s) 251, 512
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 1 cut(s) 738
BlnI CCTAGG 1 cut(s) 511
BlsI GCNGC 1 cut(s) 739
Bme1390I CCNGG 2 cut(s) 392, 693
Bme18I GGWCC 2 cut(s) 206, 533
BmgT120I GGNCC 7 cut(s) 206, 308, 486, 491, 507, 508, 533
BmiI GGNNCC 6 cut(s) 207, 208, 487, 508, 509, 534
BmrFI CCNGG 2 cut(s) 392, 693
BmsI GCATC 1 cut(s) 623
BpiI GAAGAC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 2 cut(s) 392, 693
BsaJI CCNNGG 1 cut(s) 511
Bsc4I CCNNNNNNNGG 2 cut(s) 527, 607
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 2 cut(s) 140, 638
BseLI CCNNNNNNNGG 2 cut(s) 527, 607
BseMII CTCAG 3 cut(s) 473, 486, 680
BseNI ACTGG 1 cut(s) 612
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 1 cut(s) 724
BseYI CCCAGC 1 cut(s) 376
BshFI GGCC 5 cut(s) 217, 310, 488, 492, 509
BsiHKAI GWGCWC 1 cut(s) 700
BsiSI CCGG 3 cut(s) 392, 489, 693
BslFI GGGAC 2 cut(s) 134, 192
BslI CCNNNNNNNGG 2 cut(s) 527, 607
BsmAI GTCTC 1 cut(s) 600
BsmFI GGGAC 2 cut(s) 134, 192
BsnI GGCC 5 cut(s) 217, 310, 488, 492, 509
Bsp119I TTCGAA 1 cut(s) 60
Bsp120I GGGCCC 1 cut(s) 507
Bsp1286I GDGCHC 2 cut(s) 511, 700
Bsp143I GATC 4 cut(s) 415, 576, 787, 820
BspACI CCGC 1 cut(s) 180
BspANI GGCC 5 cut(s) 217, 310, 488, 492, 509
BspCNI CTCAG 3 cut(s) 472, 487, 679
BspLI GGNNCC 6 cut(s) 207, 208, 487, 508, 509, 534
BspPI GGATC 1 cut(s) 571
BspQI GCTCTTC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 1 cut(s) 612
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 4 cut(s) 415, 576, 787, 820
BssT1I CCWWGG 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 449, 797
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 490, 518
BstDEI CTNAG 3 cut(s) 459, 495, 666
BstF5I GGATG 2 cut(s) 140, 638
BstKTI GATC 4 cut(s) 418, 579, 790, 823
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 4 cut(s) 415, 576, 787, 820
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNSI RCATGY 3 cut(s) 259, 442, 674
BstSCI CCNGG 2 cut(s) 390, 691
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 511
BstV1I GCAGC 1 cut(s) 724
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 576
BstYI RGATCY 1 cut(s) 576
BsuRI GGCC 5 cut(s) 217, 310, 488, 492, 509
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 140, 638
Cac8I GCNNGC 2 cut(s) 490, 518
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 7 cut(s) 206, 308, 486, 491, 507, 508, 533
CviAII CATG 6 cut(s) 198, 219, 256, 439, 671, 683
DdeI CTNAG 3 cut(s) 459, 495, 666
DpnI GATC 4 cut(s) 417, 578, 789, 822
DpnII GATC 4 cut(s) 415, 576, 787, 820
DraI TTTAAA 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 215
Eam1104I CTCTTC 2 cut(s) 449, 797
EarI CTCTTC 2 cut(s) 449, 797
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 511
Eco47I GGWCC 2 cut(s) 206, 533
Eco57I CTGAAG 3 cut(s) 33, 394, 778
EcoO109I RGGNCCY 4 cut(s) 206, 507, 508, 533
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 6 cut(s) 201, 222, 259, 442, 674, 686
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 134, 192
FatI CATG 6 cut(s) 197, 218, 255, 438, 670, 682
Fnu4HI GCNGC 1 cut(s) 738
FokI GGATG 2 cut(s) 127, 645
FriOI GRGCYC 1 cut(s) 511
FseI GGCCGGCC 1 cut(s) 492
Fsp4HI GCNGC 1 cut(s) 738
FspBI CTAG 2 cut(s) 251, 512
GluI GCNGC 1 cut(s) 738
GsaI CCCAGC 1 cut(s) 380
HaeIII GGCC 5 cut(s) 217, 310, 488, 492, 509
HapII CCGG 3 cut(s) 392, 489, 693
Hin1II CATG 6 cut(s) 201, 222, 259, 442, 674, 686
HincII GTYRAC 1 cut(s) 843
HindII GTYRAC 1 cut(s) 843
HinfI GANTC 3 cut(s) 566, 750, 839
HpaII CCGG 3 cut(s) 392, 489, 693
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 2 cut(s) 91, 843
Hpy188I TCNGA 1 cut(s) 462
Hpy188III TCNNGA 1 cut(s) 676
Hpy8I GTNNAC 2 cut(s) 91, 843
HpyAV CCTTC 4 cut(s) 166, 286, 366, 531
HpyCH4V TGCA 2 cut(s) 287, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 3 cut(s) 459, 495, 666
Hsp92II CATG 6 cut(s) 201, 222, 259, 442, 674, 686
KflI GGGWCCC 1 cut(s) 206
KroI GCCGGC 1 cut(s) 488
KroNI GCCGGC 1 cut(s) 490
Kzo9I GATC 4 cut(s) 415, 576, 787, 820
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 1 cut(s) 703
LpnPI CCDG 9 cut(s) 36, 362, 405, 451, 502, 507, 593, 706, 714
Lsp1109I GCAGC 1 cut(s) 724
LweI GCATC 1 cut(s) 623
MaeI CTAG 2 cut(s) 251, 512
MaeIII GTNAC 1 cut(s) 715
MalI GATC 4 cut(s) 417, 578, 789, 822
MboI GATC 4 cut(s) 415, 576, 787, 820
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 2 cut(s) 511, 700
MlsI TGGCCA 1 cut(s) 217
MluCI AATT 5 cut(s) 63, 353, 546, 764, 777
MluNI TGGCCA 1 cut(s) 217
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 4 cut(s) 464, 468, 675, 739
Mox20I TGGCCA 1 cut(s) 217
MroNI GCCGGC 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 109
MscI TGGCCA 1 cut(s) 217
MseI TTAA 6 cut(s) 122, 185, 425, 435, 573, 873
Msp20I TGGCCA 1 cut(s) 217
MspI CCGG 3 cut(s) 392, 489, 693
MspR9I CCNGG 2 cut(s) 392, 693
MwoI GCNNNNNNNGC 1 cut(s) 485
NaeI GCCGGC 1 cut(s) 490
NciI CCSGG 2 cut(s) 392, 693
NdeII GATC 4 cut(s) 415, 576, 787, 820
NgoMIV GCCGGC 1 cut(s) 488
NlaIII CATG 6 cut(s) 201, 222, 259, 442, 674, 686
NlaIV GGNNCC 6 cut(s) 207, 208, 487, 508, 509, 534
NspI RCATGY 3 cut(s) 259, 442, 674
NspV TTCGAA 1 cut(s) 60
PciI ACATGT 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 449
PdiI GCCGGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 566, 750
PflMI CCANNNNNTGG 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 390
PkrI GCNGC 1 cut(s) 739
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PpuMI RGGWCCY 2 cut(s) 206, 533
PscI ACATGT 1 cut(s) 255
Psp5II RGGWCCY 2 cut(s) 206, 533
PspFI CCCAGC 1 cut(s) 376
PspN4I GGNNCC 6 cut(s) 207, 208, 487, 508, 509, 534
PspOMI GGGCCC 1 cut(s) 507
PspPI GGNCC 7 cut(s) 206, 308, 486, 491, 507, 508, 533
PspPPI RGGWCCY 2 cut(s) 206, 533
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PsuI RGATCY 1 cut(s) 576
RigI GGCCGGCC 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 449
SaqAI TTAA 6 cut(s) 122, 185, 425, 435, 573, 873
SatI GCNGC 1 cut(s) 738
Sau3AI GATC 4 cut(s) 415, 576, 787, 820
Sau96I GGNCC 7 cut(s) 206, 308, 486, 491, 507, 508, 533
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 2 cut(s) 392, 693
SduI GDGCHC 2 cut(s) 511, 700
SetI ASST 9 cut(s) 252, 349, 425, 662, 667, 712, 731, 739, 800
SfaNI GCATC 1 cut(s) 623
SfcI CTRYAG 1 cut(s) 705
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 2 cut(s) 206, 533
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
Sse9I AATT 5 cut(s) 63, 353, 546, 764, 777
SsiI CCGC 1 cut(s) 180
SspMI CTAG 2 cut(s) 251, 512
StyD4I CCNGG 2 cut(s) 390, 691
StyI CCWWGG 1 cut(s) 511
TaqI TCGA 2 cut(s) 60, 524
TasI AATT 5 cut(s) 63, 353, 546, 764, 777
TfiI GAWTC 2 cut(s) 566, 750
Tru1I TTAA 6 cut(s) 122, 185, 425, 435, 573, 873
Tru9I TTAA 6 cut(s) 122, 185, 425, 435, 573, 873
TseI GCWGC 1 cut(s) 737
TspDTI ATGAA 5 cut(s) 17, 72, 248, 558, 742
Van91I CCANNNNNTGG 1 cut(s) 607
VpaK11BI GGWCC 2 cut(s) 206, 533
XapI RAATTY 3 cut(s) 63, 353, 777
XceI RCATGY 3 cut(s) 259, 442, 674
XmaJI CCTAGG 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 2 cut(s) 251, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.