pycom02g26200

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000151
Physical Location & Seq
Forward (+)
24041766 .. 24044099
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26200.1

Sequence Viewer

Length: 1101 bp
ATGGCTGCAGTAGAGGATACTAACAAGTTGTCTGAAGCCTATCCGATGAAGGCAGGAGATGGCCCTAACAGCTATGCCAACAACTCCACTTTTCAGAAAATAGCGGTGAATTCTTCCCAAGAAGTTGTAAGAGAGGAAATTGCAGAAAAGCTTGACATACAGACATTTTTGTTATCATCCTGCAACACCTTTCGCATTGCAGATTTAGGTTGCTCTACTGGGCCAAATACATTTTTCGCGGTTGACAACATACTCGAAACTGTGCAACTCAAGTACCAAAGCCAAGGGCTGAGTTCTCATCAAATCCCCAAATTTCAAGTTTTCTTCAACGATCAAACCACAAATGATTTTAACATGCTCTTCAAATCCCTCCCTCAGAACAGGCAATACTATGCCGCAGGTGTGCCTGGTTCGTTCTACGGTAGGATATTTCCTAATGCTTCCATTCACTTTTTTCACTGTTCTTTTTCCAATCACTGGCTTTCTAGAGTACCAAAAGAGATAGGGAACAAAGAGAGTCCAGCTTGGAATAAAGGAAAAATCTATTACTCAAGTTCCACAACTGAAGTGAAAAGGGCTTATGAAACTCAACATGCTTTGGACATAGAGCGTTTTCTTAATGCAAGGGCACAAGAGATTGTGTATGGAGGATTGATGGTGCTTATCATTCCATGTCGCCCCAATGGTACCCCTCATTCTCATACTCTGGCAAATATAACCTATGAAACTTTGGGATCTTGCCTCATAGACATGGCCAGAAAGGGAGTGGTTGATGAAGAGAAGTTAGATTCATTTAACATACCTGTGTATATCATGTCTCCCCAAGAGCTAGAAGTTGCTGTAGAAAGAACTGGATACTTTAGCATTGAGAGAATGGAAGTTCTACCAAATATGTTTCCAAATAGCAGTCTCTCTAATGCCTTATTATCTACATCTCACGTTAGAGCTGTTCATGAAGAACACATCAAGCAGCACTTTGGAGAAGAAATCATAGATGAACTCTTCAACTTATACCATAAGAAAGTTGAAGAGCGACCCTCCAAGTTTGAGTCGGGGAAGACTATTGTTTCTCTTGCCGTGCTTAAACGCAATACGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

367

Amino Acids

41.39

Weight (kDa)

6.08

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 60 - 363 3.3e-105 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37970 AT5G37990 AT5G38100 AT5G38100 AT5G38100 AT5G38100 AT5G38780 AT5G38780
fragaria_vesca FvH4_4g07940 FvH4_4g07950 FvH4_5g16970 FvH4_7g18490
malus_domestica MD02G1312100.v1.1 MD02G1312500.v1.1 MD05G1007900.v1.1 MD06G1223300.v1.1 MD06G1223500.v1.1 MD06G1223600.v1.1 MD06G1223700.v1.1 MD06G1223800.v1.1 MD06G1227300.v1.1 MD09G1253900.v1.1 MD09G1254000.v1.1 MD09G1254100.v1.1 MD09G1254200.v1.1 MD09G1254300.v1.1 MD10G1005200.v1.1 MD10G1005900.v1.1 MD14G1232400.v1.1 MD14G1232700.v1.1 MD14G1232800.v1.1 MD14G1232900.v1.1 MD14G1233000.v1.1 MD15G1255300.v1.1
prunus_persica Prupe.5G228400_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228500_v2.0.a1 Prupe.5G228600_v2.0.a1 Prupe.5G228700_v2.0.a1 Prupe.5G228800_v2.0.a1 Prupe.5G228900_v2.0.a1 Prupe.5G229000_v2.0.a1 Prupe.8G008300_v2.0.a1
pyrus_communis pycom02g26200 pycom02g26220 pycom05g00410 pycom06g19910 pycom06g19920 pycom06g19930 pycom06g19940 pycom06g20380 pycom09g16970 pycom09g16990 pycom09g17000 pycom10g00420 pycom14g19370 pycom14g19380 pycom14g19420 pycom14g19430 pycom15g22380 pycom15g22390
rosa_chinensis RchiOBHm_Chr1g0327241 RchiOBHm_Chr1g0327261 RchiOBHm_Chr2g0128631 RchiOBHm_Chr2g0137031 RchiOBHm_Chr3g0486031 RchiOBHm_Chr3g0486051 RchiOBHm_Chr3g0486091 RchiOBHm_Chr4g0400921 RchiOBHm_Chr4g0400971 RchiOBHm_Chr4g0400991 RchiOBHm_Chr7g0178281 RchiOBHm_Chr7g0178301 RchiOBHm_Chr7g0178321 RchiOBHm_Chr7g0178951 RchiOBHm_Chr7g0178961 RchiOBHm_Chr7g0178971 RchiOBHm_Chr7g0178991 RchiOBHm_Chr7g0179001 RchiOBHm_Chr7g0179021 RchiOBHm_Chr7g0179031 RchiOBHm_Chr7g0179041 RchiOBHm_Chr7g0179051 RchiOBHm_Chr7g0187641 RchiOBHm_Chr7g0201881 RchiOBHm_Chr7g0201901
rosa_laevigata RLG00000005390 RLG00000005391 RLG00000005392 RLG00000005394 RLG00000005456 RLG00000009163 RLG00000009164 RLG00000019598 RLG00000023105 RLG00000030028
rosa_multiflora Rmu_co8337425.1_g000001 Rmu_sc0000795.1_g000125 Rmu_sc0000837.1_g000085 Rmu_sc0000888.1_g000008 Rmu_sc0000888.1_g000010 Rmu_sc0000888.1_g000011 Rmu_sc0000888.1_g000013 Rmu_sc0001021.1_g000003 Rmu_sc0001021.1_g000004 Rmu_sc0001021.1_g000006 Rmu_sc0001374.1_g000046 Rmu_sc0002026.1_g000007 Rmu_sc0002026.1_g000019 Rmu_sc0003465.1_g000046 Rmu_sc0004483.1_g000006 Rmu_sc0006229.1_g000002 Rmu_sc0006736.1_g000019 Rmu_sc0006736.1_g000027 Rmu_sc0007073.1_g000003 Rmu_sc0036373.1_g000001 Rmu_ssc0000009.1_g000007
rosa_roxburghii Rroxscaffold_2G00107760 Rroxscaffold_2G00115440 Rroxscaffold_2G00115920 Rroxscaffold_3G00255030 Rroxscaffold_3G00267200 Rroxscaffold_3G00274440 Rroxscaffold_3G00274450 Rroxscaffold_3G00274480 Rroxscaffold_4G00322940 Rroxscaffold_4G00322950 Rroxscaffold_4G00322980 Rroxscaffold_4G00323000 Rroxscaffold_4G00323020 Rroxscaffold_5G00345860 Rroxscaffold_5G00345890 Rroxscaffold_5G00345920 Rroxscaffold_6G00396060 Rroxscaffold_6G00396070
rosa_rugosa Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064700 Rorug01G0064800 Rorug01G0297000 Rorug02G0279500 Rorug02G0337300 Rorug03G0223300 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223400 Rorug03G0223500 Rorug03G0223600 Rorug04G0027400 Rorug04G0027500 Rorug04G0027600 Rorug06G0420600 Rorug06G0420600 Rorug06G0483400 Rorug07G0066400 Rorug07G0066600
rosa_samantha Rh1AG081900 Rh1AG082300 Rh1AG082400 Rh1AG305600 Rh2BG340800 Rh2BG393000 Rh3BG308300 Rh4CG113300 Rh4CG113700 Rh7CG020600 Rh7CG020700 Rh7CG020800 Rh7CG021000 Rh7CG088900 Rh7CG204900
rosa_wichuraiana Rw0G002670 Rw0G014940 Rw0G021450 Rw1G006470 Rw1G006490 Rw2G026880 Rw2G027140 Rw2G031610 Rw3G024210 Rw3G024220 Rw7G001620 Rw7G001630 Rw7G001640 Rw7G001650 Rw7G016930 Rw7G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
Acc65I GGTACC 1 cut(s) 686
AccB1I GGYRCC 1 cut(s) 686
AccB7I CCANNNNNTGG 1 cut(s) 477
AccII CGCG 1 cut(s) 239
AciI CCGC 3 cut(s) 104, 239, 396
AclWI GGATC 1 cut(s) 742
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 2 cut(s) 109, 311
AcuI CTGAAG 2 cut(s) 54, 585
AfaI GTAC 3 cut(s) 275, 492, 688
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 5 cut(s) 317, 328, 364, 1006, 1028
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 5 cut(s) 72, 151, 524, 829, 947
AluI AGCT 5 cut(s) 72, 151, 524, 829, 947
Alw26I GTCTC 2 cut(s) 822, 914
AlwI GGATC 1 cut(s) 742
AoxI GGCC 3 cut(s) 61, 221, 753
ApeKI GCWGC 2 cut(s) 5, 970
ApoI RAATTY 2 cut(s) 109, 311
Asp718I GGTACC 1 cut(s) 686
AspS9I GGNCC 2 cut(s) 62, 221
AsuHPI GGTGA 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 631
BaeI ACNNNNGTAYC 2 cut(s) 257, 290
BalI TGGCCA 1 cut(s) 755
BanI GGYRCC 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 1064
BbvI GCAGC 1 cut(s) 982
BccI CCATC 2 cut(s) 53, 649
BceAI ACGGC 1 cut(s) 1061
BciT130I CCWGG 1 cut(s) 408
BciVI GTATCC 2 cut(s) 10, 848
BcoDI GTCTC 2 cut(s) 822, 914
BfaI CTAG 2 cut(s) 486, 830
BfmI CTRYAG 2 cut(s) 6, 840
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 2 cut(s) 10, 848
BisI GCNGC 3 cut(s) 6, 396, 971
BlsI GCNGC 3 cut(s) 7, 397, 972
Bme1390I CCNGG 1 cut(s) 408
BmgT120I GGNCC 2 cut(s) 62, 221
BmiI GGNNCC 1 cut(s) 688
BmrFI CCNGG 1 cut(s) 408
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 1064
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpuEI CTTGAG 2 cut(s) 254, 535
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 3 cut(s) 223, 482, 856
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 2 cut(s) 281, 389
BseNI ACTGG 3 cut(s) 223, 482, 856
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 1 cut(s) 982
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 3 cut(s) 63, 223, 755
BshNI GGYRCC 1 cut(s) 686
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 2 cut(s) 822, 914
BsnI GGCC 3 cut(s) 63, 223, 755
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 2 cut(s) 331, 734
BspACI CCGC 3 cut(s) 104, 239, 396
BspANI GGCC 3 cut(s) 63, 223, 755
BspCNI CTCAG 2 cut(s) 282, 388
BspFNI CGCG 1 cut(s) 239
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 1 cut(s) 688
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 1 cut(s) 742
BspQI GCTCTTC 2 cut(s) 365, 1023
BspT107I GGYRCC 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 3 cut(s) 223, 482, 856
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 2 cut(s) 331, 734
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 262, 422, 461
Bst6I CTCTTC 4 cut(s) 365, 771, 1007, 1023
BstDEI CTNAG 2 cut(s) 290, 375
BstF5I GGATG 1 cut(s) 176
BstFNI CGCG 1 cut(s) 239
BstKTI GATC 2 cut(s) 334, 737
BstMAI GTCTC 2 cut(s) 822, 914
BstMBI GATC 2 cut(s) 331, 734
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 2 cut(s) 358, 596
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 2 cut(s) 6, 840
BstSLI GKGCMC 1 cut(s) 631
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 982
BstV2I GAAGAC 1 cut(s) 1064
BstX2I RGATCY 1 cut(s) 734
BstYI RGATCY 1 cut(s) 734
BsuI GTATCC 2 cut(s) 10, 848
BsuRI GGCC 3 cut(s) 63, 223, 755
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 2 cut(s) 457, 475
BveI ACCTGC 1 cut(s) 389
CciI TCATGA 1 cut(s) 952
Cfr13I GGNCC 2 cut(s) 62, 221
Csp6I GTAC 3 cut(s) 274, 491, 687
CviAII CATG 6 cut(s) 355, 593, 672, 751, 814, 953
CviQI GTAC 3 cut(s) 274, 491, 687
DdeI CTNAG 2 cut(s) 290, 375
DpnI GATC 2 cut(s) 333, 736
DpnII GATC 2 cut(s) 331, 734
EaeI YGGCCR 1 cut(s) 753
Eam1104I CTCTTC 4 cut(s) 365, 771, 1007, 1023
EarI CTCTTC 4 cut(s) 365, 771, 1007, 1023
Eco130I CCWWGG 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 54, 585
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 6 cut(s) 358, 596, 675, 754, 817, 956
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FatI CATG 6 cut(s) 354, 592, 671, 750, 813, 952
Fnu4HI GCNGC 3 cut(s) 6, 396, 971
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 3 cut(s) 6, 396, 971
FspBI CTAG 2 cut(s) 486, 830
GluI GCNGC 3 cut(s) 6, 396, 971
HaeIII GGCC 3 cut(s) 63, 223, 755
Hin1II CATG 6 cut(s) 358, 596, 675, 754, 817, 956
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 3 cut(s) 517, 788, 1049
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 4 cut(s) 34, 45, 96, 378
Hpy188III TCNNGA 2 cut(s) 486, 953
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 3 cut(s) 262, 422, 461
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 6 cut(s) 8, 143, 183, 200, 265, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 2 cut(s) 290, 375
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 6 cut(s) 358, 596, 675, 754, 817, 956
KpnI GGTACC 1 cut(s) 690
Kzo9I GATC 2 cut(s) 331, 734
LguI GCTCTTC 2 cut(s) 365, 1023
Lsp1109I GCAGC 1 cut(s) 982
MaeI CTAG 2 cut(s) 486, 830
MaeII ACGT 1 cut(s) 939
MalI GATC 2 cut(s) 333, 736
MboI GATC 2 cut(s) 331, 734
MboII GAAGA 9 cut(s) 105, 316, 352, 788, 968, 994, 995, 1040, 1069
MflI RGATCY 1 cut(s) 734
MhlI GDGCHC 1 cut(s) 631
MlsI TGGCCA 1 cut(s) 755
MluCI AATT 4 cut(s) 109, 138, 311, 1096
MluNI TGGCCA 1 cut(s) 755
MlyI GAGTC 2 cut(s) 526, 1058
MnlI CCTC 8 cut(s) 7, 127, 380, 384, 641, 702, 752, 1048
Mox20I TGGCCA 1 cut(s) 755
MscI TGGCCA 1 cut(s) 755
MseI TTAA 5 cut(s) 351, 618, 795, 1083, 1099
MslI CAYNNNNRTG 2 cut(s) 749, 803
Msp20I TGGCCA 1 cut(s) 755
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 2 cut(s) 331, 734
NlaIII CATG 6 cut(s) 358, 596, 675, 754, 817, 956
NlaIV GGNNCC 1 cut(s) 688
NspI RCATGY 2 cut(s) 358, 596
PagI TCATGA 1 cut(s) 952
PaqCI CACCTGC 1 cut(s) 389
PciSI GCTCTTC 2 cut(s) 365, 1023
PfeI GAWTC 1 cut(s) 788
PflMI CCANNNNNTGG 1 cut(s) 477
PkrI GCNGC 3 cut(s) 7, 397, 972
PleI GAGTC 2 cut(s) 525, 1057
PpsI GAGTC 2 cut(s) 525, 1057
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 688
PspPI GGNCC 2 cut(s) 62, 221
PsrI GAACNNNNNNTAC 2 cut(s) 371, 403
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 734
RsaI GTAC 3 cut(s) 275, 492, 688
RsaNI GTAC 3 cut(s) 274, 491, 687
RseI CAYNNNNRTG 2 cut(s) 749, 803
SapI GCTCTTC 2 cut(s) 365, 1023
SaqAI TTAA 5 cut(s) 351, 618, 795, 1083, 1099
SatI GCNGC 3 cut(s) 6, 396, 971
Sau3AI GATC 2 cut(s) 331, 734
Sau96I GGNCC 2 cut(s) 62, 221
SchI GAGTC 2 cut(s) 526, 1058
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 631
SfcI CTRYAG 2 cut(s) 6, 840
SmiMI CAYNNNNRTG 2 cut(s) 749, 803
SmlI CTYRAG 2 cut(s) 269, 550
SmoI CTYRAG 2 cut(s) 269, 550
Sse9I AATT 4 cut(s) 109, 138, 311, 1096
SsiI CCGC 3 cut(s) 104, 239, 396
SspMI CTAG 2 cut(s) 486, 830
StyD4I CCNGG 1 cut(s) 406
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 262, 422, 461
TaiI ACGT 1 cut(s) 942
TaqI TCGA 1 cut(s) 255
TasI AATT 4 cut(s) 109, 138, 311, 1096
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 1 cut(s) 788
Tru1I TTAA 5 cut(s) 351, 618, 795, 1083, 1099
Tru9I TTAA 5 cut(s) 351, 618, 795, 1083, 1099
TscAI CASTG 2 cut(s) 464, 482
TseI GCWGC 2 cut(s) 5, 970
TspDTI ATGAA 8 cut(s) 62, 597, 738, 780, 789, 941, 969, 1011
TspRI CASTG 2 cut(s) 464, 482
Van91I CCANNNNNTGG 1 cut(s) 477
XapI RAATTY 2 cut(s) 109, 311
XbaI TCTAGA 1 cut(s) 485
XceI RCATGY 2 cut(s) 358, 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XspI CTAG 2 cut(s) 486, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.