methyltransferase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 267, 380 |
| AclWI | GGATC | 1 cut(s) | 709 |
| AcsI | RAATTY | 2 cut(s) | 251, 293 |
| AcuI | CTGAAG | 1 cut(s) | 292 |
| AcyI | GRCGYC | 1 cut(s) | 658 |
| AfaI | GTAC | 1 cut(s) | 474 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 459, 718 |
| AgsI | TTSAA | 8 cut(s) | 146, 169, 230, 242, 299, 346, 556, 677 |
| AjnI | CCWGG | 2 cut(s) | 388, 711 |
| AluBI | AGCT | 4 cut(s) | 63, 174, 245, 569 |
| AluI | AGCT | 4 cut(s) | 63, 174, 245, 569 |
| Alw21I | GWGCWC | 1 cut(s) | 613 |
| Alw26I | GTCTC | 1 cut(s) | 611 |
| AlwI | GGATC | 1 cut(s) | 709 |
| AoxI | GGCC | 3 cut(s) | 52, 206, 272 |
| ApeKI | GCWGC | 1 cut(s) | 377 |
| ApoI | RAATTY | 2 cut(s) | 251, 293 |
| AspS9I | GGNCC | 3 cut(s) | 53, 206, 272 |
| Bbv12I | GWGCWC | 1 cut(s) | 613 |
| BbvI | GCAGC | 1 cut(s) | 389 |
| BccI | CCATC | 2 cut(s) | 44, 712 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 740 |
| BciT130I | CCWGG | 2 cut(s) | 390, 713 |
| BcoDI | GTCTC | 1 cut(s) | 611 |
| BfaI | CTAG | 4 cut(s) | 468, 614, 705, 723 |
| BfmI | CTRYAG | 1 cut(s) | 78 |
| BisI | GCNGC | 1 cut(s) | 378 |
| BlsI | GCNGC | 1 cut(s) | 379 |
| Bme1390I | CCNGG | 2 cut(s) | 390, 713 |
| BmgT120I | GGNCC | 3 cut(s) | 53, 206, 272 |
| BmiI | GGNNCC | 1 cut(s) | 55 |
| BmrFI | CCNGG | 2 cut(s) | 390, 713 |
| BmsI | GCATC | 3 cut(s) | 91, 634, 748 |
| BpmI | CTGGAG | 1 cut(s) | 734 |
| BsaHI | GRCGYC | 1 cut(s) | 658 |
| BsaJI | CCNNGG | 3 cut(s) | 400, 417, 579 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 459, 718 |
| Bse1I | ACTGG | 2 cut(s) | 12, 737 |
| BseBI | CCWGG | 2 cut(s) | 390, 713 |
| BseDI | CCNNGG | 3 cut(s) | 400, 417, 579 |
| BseGI | GGATG | 1 cut(s) | 763 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 459, 718 |
| BseMII | CTCAG | 1 cut(s) | 15 |
| BseNI | ACTGG | 2 cut(s) | 12, 737 |
| BseXI | GCAGC | 1 cut(s) | 389 |
| Bsh1285I | CGRYCG | 1 cut(s) | 406 |
| BshFI | GGCC | 3 cut(s) | 54, 208, 274 |
| BsiEI | CGRYCG | 1 cut(s) | 406 |
| BsiHKAI | GWGCWC | 1 cut(s) | 613 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 459, 718 |
| BsmAI | GTCTC | 1 cut(s) | 611 |
| BsnI | GGCC | 3 cut(s) | 54, 208, 274 |
| Bsp1286I | GDGCHC | 1 cut(s) | 613 |
| Bsp143I | GATC | 1 cut(s) | 701 |
| BspACI | CCGC | 2 cut(s) | 267, 380 |
| BspANI | GGCC | 3 cut(s) | 54, 208, 274 |
| BspCNI | CTCAG | 1 cut(s) | 16 |
| BspLI | GGNNCC | 1 cut(s) | 55 |
| BspPI | GGATC | 1 cut(s) | 709 |
| BsrI | ACTGG | 2 cut(s) | 12, 737 |
| BssECI | CCNNGG | 3 cut(s) | 400, 417, 579 |
| BssMI | GATC | 1 cut(s) | 701 |
| BssNI | GRCGYC | 1 cut(s) | 658 |
| BssT1I | CCWWGG | 1 cut(s) | 417 |
| Bst2UI | CCWGG | 2 cut(s) | 390, 713 |
| Bst4CI | ACNGT | 4 cut(s) | 404, 500, 622, 671 |
| Bst6I | CTCTTC | 2 cut(s) | 347, 448 |
| BstACI | GRCGYC | 1 cut(s) | 658 |
| BstDEI | CTNAG | 2 cut(s) | 24, 119 |
| BstDSI | CCRYGG | 1 cut(s) | 400 |
| BstF5I | GGATG | 1 cut(s) | 763 |
| BstKTI | GATC | 1 cut(s) | 704 |
| BstMAI | GTCTC | 1 cut(s) | 611 |
| BstMBI | GATC | 1 cut(s) | 701 |
| BstMCI | CGRYCG | 1 cut(s) | 406 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 60, 566, 575 |
| BstNI | CCWGG | 2 cut(s) | 390, 713 |
| BstSCI | CCNGG | 2 cut(s) | 388, 711 |
| BstSFI | CTRYAG | 1 cut(s) | 78 |
| BstV1I | GCAGC | 1 cut(s) | 389 |
| BstX2I | RGATCY | 1 cut(s) | 701 |
| BstYI | RGATCY | 1 cut(s) | 701 |
| BsuRI | GGCC | 3 cut(s) | 54, 208, 274 |
| BtgI | CCRYGG | 1 cut(s) | 400 |
| BtsCI | GGATG | 1 cut(s) | 763 |
| BtsIMutI | CAGTG | 1 cut(s) | 316 |
| Cfr13I | GGNCC | 3 cut(s) | 53, 206, 272 |
| CseI | GACGC | 1 cut(s) | 666 |
| Csp6I | GTAC | 1 cut(s) | 473 |
| CviAII | CATG | 1 cut(s) | 586 |
| CviQI | GTAC | 1 cut(s) | 473 |
| DdeI | CTNAG | 2 cut(s) | 24, 119 |
| DpnI | GATC | 1 cut(s) | 703 |
| DpnII | GATC | 1 cut(s) | 701 |
| Eam1104I | CTCTTC | 2 cut(s) | 347, 448 |
| EarI | CTCTTC | 2 cut(s) | 347, 448 |
| Eco130I | CCWWGG | 1 cut(s) | 417 |
| Eco57I | CTGAAG | 1 cut(s) | 292 |
| EcoRII | CCWGG | 2 cut(s) | 388, 711 |
| EcoT14I | CCWWGG | 1 cut(s) | 417 |
| ErhI | CCWWGG | 1 cut(s) | 417 |
| FaeI | CATG | 1 cut(s) | 589 |
| FaiI | YATR | 9 cut(s) | 31, 66, 180, 236, 375, 562, 576, 587, 769 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 126, 158 |
| FatI | CATG | 1 cut(s) | 585 |
| FauI | CCCGC | 1 cut(s) | 373 |
| Fnu4HI | GCNGC | 1 cut(s) | 378 |
| Fsp4HI | GCNGC | 1 cut(s) | 378 |
| FspBI | CTAG | 4 cut(s) | 468, 614, 705, 723 |
| GluI | GCNGC | 1 cut(s) | 378 |
| GsuI | CTGGAG | 1 cut(s) | 734 |
| HaeIII | GGCC | 3 cut(s) | 54, 208, 274 |
| HgaI | GACGC | 1 cut(s) | 666 |
| Hin1I | GRCGYC | 1 cut(s) | 658 |
| Hin1II | CATG | 1 cut(s) | 589 |
| HinfI | GANTC | 2 cut(s) | 8, 522 |
| Hpy188I | TCNGA | 3 cut(s) | 25, 283, 360 |
| Hpy188III | TCNNGA | 2 cut(s) | 143, 468 |
| Hpy99I | CGWCG | 1 cut(s) | 548 |
| HpyAV | CCTTC | 2 cut(s) | 464, 550 |
| HpyCH4III | ACNGT | 4 cut(s) | 404, 500, 622, 671 |
| HpyCH4V | TGCA | 3 cut(s) | 185, 377, 625 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 60, 566, 575 |
| HpyF3I | CTNAG | 2 cut(s) | 24, 119 |
| Hsp92I | GRCGYC | 1 cut(s) | 658 |
| Hsp92II | CATG | 1 cut(s) | 589 |
| Kzo9I | GATC | 1 cut(s) | 701 |
| LpnPI | CCDG | 8 cut(s) | 25, 349, 375, 402, 619, 698, 718, 725 |
| Lsp1109I | GCAGC | 1 cut(s) | 389 |
| LweI | GCATC | 3 cut(s) | 91, 634, 748 |
| MaeI | CTAG | 4 cut(s) | 468, 614, 705, 723 |
| MalI | GATC | 1 cut(s) | 703 |
| MboI | GATC | 1 cut(s) | 701 |
| MboII | GAAGA | 4 cut(s) | 298, 334, 435, 739 |
| MflI | RGATCY | 1 cut(s) | 701 |
| MhlI | GDGCHC | 1 cut(s) | 613 |
| MluCI | AATT | 5 cut(s) | 146, 251, 293, 346, 678 |
| MnlI | CCTC | 9 cut(s) | 19, 263, 362, 366, 574, 608, 623, 672, 719 |
| MseI | TTAA | 6 cut(s) | 132, 333, 429, 645, 666, 690 |
| MspA1I | CMGCKG | 1 cut(s) | 380 |
| MspR9I | CCNGG | 2 cut(s) | 390, 713 |
| MvaI | CCWGG | 2 cut(s) | 390, 713 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 60, 566, 575 |
| NdeII | GATC | 1 cut(s) | 701 |
| NlaIII | CATG | 1 cut(s) | 589 |
| NlaIV | GGNNCC | 1 cut(s) | 55 |
| NmeAIII | GCCGAG | 2 cut(s) | 300, 604 |
| PfeI | GAWTC | 2 cut(s) | 8, 522 |
| PfoI | TCCNGGA | 1 cut(s) | 711 |
| PkrI | GCNGC | 1 cut(s) | 379 |
| Psp6I | CCWGG | 2 cut(s) | 388, 711 |
| PspGI | CCWGG | 2 cut(s) | 388, 711 |
| PspN4I | GGNNCC | 1 cut(s) | 55 |
| PspPI | GGNCC | 3 cut(s) | 53, 206, 272 |
| PsuI | RGATCY | 1 cut(s) | 701 |
| RsaI | GTAC | 1 cut(s) | 474 |
| RsaNI | GTAC | 1 cut(s) | 473 |
| SaqAI | TTAA | 6 cut(s) | 132, 333, 429, 645, 666, 690 |
| SatI | GCNGC | 1 cut(s) | 378 |
| Sau3AI | GATC | 1 cut(s) | 701 |
| Sau96I | GGNCC | 3 cut(s) | 53, 206, 272 |
| ScrFI | CCNGG | 2 cut(s) | 390, 713 |
| SduI | GDGCHC | 1 cut(s) | 613 |
| SetI | ASST | 6 cut(s) | 65, 176, 247, 394, 571, 651 |
| SfaNI | GCATC | 3 cut(s) | 91, 634, 748 |
| SfcI | CTRYAG | 1 cut(s) | 78 |
| Sse9I | AATT | 5 cut(s) | 146, 251, 293, 346, 678 |
| SsiI | CCGC | 2 cut(s) | 267, 380 |
| SspMI | CTAG | 4 cut(s) | 468, 614, 705, 723 |
| StyD4I | CCNGG | 2 cut(s) | 388, 711 |
| StyI | CCWWGG | 1 cut(s) | 417 |
| TaaI | ACNGT | 4 cut(s) | 404, 500, 622, 671 |
| TaqI | TCGA | 1 cut(s) | 628 |
| TasI | AATT | 5 cut(s) | 146, 251, 293, 346, 678 |
| TfiI | GAWTC | 2 cut(s) | 8, 522 |
| Tru1I | TTAA | 6 cut(s) | 132, 333, 429, 645, 666, 690 |
| Tru9I | TTAA | 6 cut(s) | 132, 333, 429, 645, 666, 690 |
| TscAI | CASTG | 1 cut(s) | 316 |
| TseI | GCWGC | 1 cut(s) | 377 |
| TspDTI | ATGAA | 3 cut(s) | 53, 435, 446 |
| TspGWI | ACGGA | 1 cut(s) | 590 |
| TspRI | CASTG | 1 cut(s) | 316 |
| XapI | RAATTY | 2 cut(s) | 251, 293 |
| XbaI | TCTAGA | 1 cut(s) | 467 |
| XspI | CTAG | 4 cut(s) | 468, 614, 705, 723 |