Rw4G004440

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000555
Physical Location & Seq
Reverse (-)
8876627 .. 8886106
9480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004440.1

Sequence Viewer

Length: 2592 bp
ATGAAGGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGCGTTGAAGTGGTTCTATATGAGAAAAAAGACTACATGGGTGGCCATGCCAGGACTGTCACATATGATGGTGTTGATTTGGACCTTGGTCTCATGGTCTTCAATCATGTGATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGCGACATGTCCTTCTCAGCGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCGAAATAGTCTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCTTGAGAGTAACCCAGACATTGACCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTCTGGAGGCTTACCTTATTCCAATATGTAGTGCAATCTTGTTGTGTCCTTCAGAAGGAGTTATAAGCTTCTCAGCTTTCTCAGTGCTTTCGGTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCGGCCCACAATGGCTTACCATCAAATGGGGTTCACATTGTTATGTGAAGAAGGTGAAAGAAATGTTGGAGAGTAAAGGGTGTCAGATAAGAACTGGCTGTGAGGTGCATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTTTTGAGGAAATATACGATGGATGCATTATAGCTATCCAAGCCCCCGATGCCATGAGAATGTTAGGAGAACAGGCAACACCTGATGAACTGAGAATACTTGGCGCCTTCCAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCGAAATCTAGCAGCATGGAGTACATTAAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGAACCTCGGTGAAACAAATCAACCATATCTTGTGACACTCAATCCGGATCATACACCGAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCTGCATCGAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTATTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGGCTGGAGTGGCTGCAGCACATGGTATGCTTGGAAAAGGTTGTGCCCTGTTGGGAAACCCAAAACATATGGCTCTTTCATTGACGGAAACAGCAGCACGCCTATTTGTTACTAGATTTTTTAGACATTATATATCTACCGGATATTTAGTCTTATTAGAAGAGGGAGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGGCTGTTCTCTAAAATCCATTCTTAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATCAGTGGTGATTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTCATTGCCAACAGAGATTCTAATGCCTTAAAATCAAACAAGAAAAGGGGATGGTGGCAGCCTTTGTTTATCACAGCCACTATAGCGTCAGCCAAATGTGTCTTTCAGCATGCTTTAAGGAAAAATAGTCTTACACAGGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCGCTGTTCTTGGGTGAAACGATGATGTACTCCACTGCAATATTTAAGACAGAAGATGAGGATTTGAATACTGCACAGCTTAGGAAAATCTCTATTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTTGATATTGGTTGTGGTTGGGGAACCTTTGCTATTGAAGCAGTCAAACAAACGGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAGGTGAAAAATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTTACCACAAATATGACAGAATCATATCTTGTGAGATGTTAGAACATGTCGGCCATGAATTTATGGACGAGTTCTTTTCTTGCTGTGAGTCTGTACTGGCAAAAAATGGGCTTTTTATTCTCCAGTTCATATCTATGCCGGATGAATTCTATGATGAGTACCTACGAAGTTCAGGCTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACCGTCATTAAGTAGGGTAACATCAGCCATGGCCACTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGAATTCATTATTATCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGAGAGACAGAGTGAAATACTCGCTCTTGGATTCAATGAAAATTTCATTCGAACATGGGAATATTATTTTGATTATTGTGCTGCTGGTTTCAAGTCACATACACTTGGAGACTACCAGATTGTGTTTTCACGTCCTGGTAATGCTCTCACATTCAACAATCCACATAAGGGTTTCCCTTCAGCATATTGA

Protein Analysis

863

Amino Acids

97.89

Weight (kDa)

6.33

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 7.6e-07 FAD dependent oxidoreductase
FAD_binding_2 PF00890 3 - 39 1e-05 FAD binding domain
NAD_binding_8 PF13450 5 - 71 1.5e-16 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 273 6.5e-21 Flavin containing amine oxidoreductase
CMAS PF02353 568 - 836 3.9e-80 Mycolic acid cyclopropane synthetase
MTS PF05175 619 - 729 3.6e-06 Methyltransferase small domain
Methyltransf_23 PF13489 623 - 737 6.6e-10 Methyltransferase domain
Methyltransf_31 PF13847 626 - 739 1.2e-14 Methyltransferase domain
Methyltransf_25 PF13649 631 - 727 2e-17 Methyltransferase domain
Methyltransf_11 PF08241 632 - 731 1.7e-14 Methyltransferase domain
Methyltransf_12 PF08242 632 - 729 5.1e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 633
AccI GTMKAC 1 cut(s) 2281
AccIII TCCGGA 1 cut(s) 1054
AciI CCGC 2 cut(s) 609, 2059
AclWI GGATC 2 cut(s) 1065, 2042
AcoI YGGCCR 3 cut(s) 109, 2122, 2313
AcsI RAATTY 7 cut(s) 1167, 1991, 2129, 2216, 2358, 2395, 2443
AcuI CTGAAG 5 cut(s) 516, 581, 992, 2009, 2565
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 6 cut(s) 884, 946, 967, 1787, 2166, 2231
AfiI CCNNNNNNNGG 3 cut(s) 536, 633, 2570
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 2 cut(s) 237, 2116
AjiI CACGTC 1 cut(s) 2534
AjnI CCWGG 4 cut(s) 116, 1188, 2269, 2536
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 656, 688
AluBI AGCT 9 cut(s) 306, 549, 557, 601, 797, 1006, 1130, 1141, 1837
AluI AGCT 9 cut(s) 306, 549, 557, 601, 797, 1006, 1130, 1141, 1837
Alw21I GWGCWC 1 cut(s) 2343
Alw26I GTCTC 6 cut(s) 46, 161, 221, 302, 2401, 2505
AlwI GGATC 2 cut(s) 1065, 2042
Aor13HI TCCGGA 1 cut(s) 1054
AoxI GGCC 4 cut(s) 109, 610, 2122, 2313
ApeKI GCWGC 8 cut(s) 935, 1118, 1232, 1235, 1313, 1630, 1950, 2483
ApoI RAATTY 7 cut(s) 1167, 1991, 2129, 2216, 2358, 2395, 2443
Asp700I GAANNNNTTC 3 cut(s) 78, 329, 480
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 2 cut(s) 148, 611
AsuHPI GGTGA 7 cut(s) 358, 673, 1031, 1390, 1532, 1784, 2020
AsuII TTCGAA 1 cut(s) 2452
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 1267
BalI TGGCCA 2 cut(s) 111, 2315
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 157, 1544
Bbv12I GWGCWC 1 cut(s) 2343
BbvI GCAGC 8 cut(s) 947, 1105, 1219, 1247, 1325, 1642, 1937, 2470
BccI CCATC 8 cut(s) 128, 187, 635, 755, 776, 1107, 1205, 1617
BciT130I CCWGG 4 cut(s) 118, 1190, 2271, 2538
BciVI GTATCC 1 cut(s) 192
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 6 cut(s) 46, 161, 221, 302, 2401, 2505
BfaI CTAG 5 cut(s) 932, 1332, 1493, 1869, 2325
BfmI CTRYAG 3 cut(s) 1233, 1653, 1951
BfoI RGCGCY 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 9 cut(s) 610, 936, 1119, 1233, 1236, 1314, 1631, 1951, 2484
BlsI GCNGC 9 cut(s) 611, 937, 1120, 1234, 1237, 1315, 1632, 1952, 2485
BmcAI AGTACT 1 cut(s) 967
Bme1390I CCNGG 4 cut(s) 118, 1190, 2271, 2538
Bme18I GGWCC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 2534
BmgT120I GGNCC 2 cut(s) 148, 611
BmiI GGNNCC 2 cut(s) 868, 1918
BmrFI CCNGG 4 cut(s) 118, 1190, 2271, 2538
BmsI GCATC 5 cut(s) 776, 802, 1130, 1495, 1974
BoxI GACNNNNGTC 2 cut(s) 153, 1548
BpiI GAAGAC 2 cut(s) 157, 1544
BpmI CTGGAG 5 cut(s) 506, 777, 1157, 1245, 2177
Bpu10I CCTNAGC 1 cut(s) 1838
Bpu14I TTCGAA 1 cut(s) 2452
BpuEI CTTGAG 3 cut(s) 286, 419, 428
Bsa29I ATCGAT 1 cut(s) 1875
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 2 cut(s) 46, 161
BsaJI CCNNGG 7 cut(s) 151, 211, 441, 1015, 1189, 1546, 2310
BsaWI WCCGGW 4 cut(s) 429, 1054, 1358, 2031
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 633, 2570
Bse1I ACTGG 8 cut(s) 706, 760, 998, 1103, 1472, 1966, 2172, 2194
Bse3DI GCAATG 2 cut(s) 1497, 1575
BseAI TCCGGA 1 cut(s) 1054
BseBI CCWGG 4 cut(s) 118, 1190, 2271, 2538
BseCI ATCGAT 1 cut(s) 1875
BseDI CCNNGG 7 cut(s) 151, 211, 441, 1015, 1189, 1546, 2310
BseGI GGATG 6 cut(s) 791, 1099, 1216, 1628, 2218, 2282
BseLI CCNNNNNNNGG 3 cut(s) 536, 633, 2570
BseMI GCAATG 2 cut(s) 1497, 1575
BseMII CTCAG 8 cut(s) 57, 261, 567, 576, 845, 1472, 1733, 1968
BseNI ACTGG 8 cut(s) 706, 760, 998, 1103, 1472, 1966, 2172, 2194
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 8 cut(s) 947, 1105, 1219, 1247, 1325, 1642, 1937, 2470
BsgI GTGCAG 1 cut(s) 1815
BshFI GGCC 4 cut(s) 111, 612, 2124, 2315
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1875
BsiHKAI GWGCWC 1 cut(s) 2343
BsiSI CCGG 5 cut(s) 430, 1055, 1359, 2032, 2210
BslI CCNNNNNNNGG 3 cut(s) 536, 633, 2570
BsmAI GTCTC 6 cut(s) 46, 161, 221, 302, 2401, 2505
BsnI GGCC 4 cut(s) 111, 612, 2124, 2315
Bso31I GGTCTC 2 cut(s) 46, 161
Bsp119I TTCGAA 1 cut(s) 2452
Bsp1286I GDGCHC 2 cut(s) 1267, 2343
Bsp13I TCCGGA 1 cut(s) 1054
Bsp143I GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
Bsp19I CCATGG 1 cut(s) 2310
BspACI CCGC 2 cut(s) 609, 2059
BspANI GGCC 4 cut(s) 111, 612, 2124, 2315
BspCNI CTCAG 8 cut(s) 56, 260, 566, 575, 846, 1471, 1734, 1969
BspDI ATCGAT 1 cut(s) 1875
BspEI TCCGGA 1 cut(s) 1054
BspLI GGNNCC 2 cut(s) 868, 1918
BspMAI CTGCAG 2 cut(s) 1237, 1955
BspPI GGATC 2 cut(s) 1065, 2042
BspT104I TTCGAA 1 cut(s) 2452
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 2 cut(s) 46, 161
BsrDI GCAATG 2 cut(s) 1497, 1575
BsrI ACTGG 8 cut(s) 706, 760, 998, 1103, 1472, 1966, 2172, 2194
BssECI CCNNGG 7 cut(s) 151, 211, 441, 1015, 1189, 1546, 2310
BssMI GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 4 cut(s) 151, 211, 441, 2310
Bst2UI CCWGG 4 cut(s) 118, 1190, 2271, 2538
Bst4CI ACNGT 5 cut(s) 124, 748, 887, 1454, 2286
Bst6I CTCTTC 1 cut(s) 1374
BstACI GRCGYC 1 cut(s) 867
BstAFI CTTAAG 1 cut(s) 1082
BstBI TTCGAA 1 cut(s) 2452
BstC8I GCNNGC 2 cut(s) 1318, 1683
BstDSI CCRYGG 1 cut(s) 2310
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 6 cut(s) 791, 1099, 1216, 1628, 2218, 2282
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 5 cut(s) 390, 1060, 1492, 1741, 2037
BstMAI GTCTC 6 cut(s) 46, 161, 221, 302, 2401, 2505
BstMBI GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
BstMWI GCNNNNNNNGC 8 cut(s) 803, 812, 1003, 1127, 1229, 1419, 1655, 1931
BstNI CCWGG 4 cut(s) 118, 1190, 2271, 2538
BstNSI RCATGY 4 cut(s) 234, 241, 1685, 2120
BstPAI GACNNNNGTC 2 cut(s) 153, 1548
BstSCI CCNGG 4 cut(s) 116, 1188, 2269, 2536
BstSFI CTRYAG 3 cut(s) 1233, 1653, 1951
BstSLI GKGCMC 1 cut(s) 1267
BstV1I GCAGC 8 cut(s) 947, 1105, 1219, 1247, 1325, 1642, 1937, 2470
BstV2I GAAGAC 2 cut(s) 157, 1544
BstXI CCANNNNNNTGG 3 cut(s) 345, 1196, 1211
Bsu15I ATCGAT 1 cut(s) 1875
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 4 cut(s) 111, 612, 2124, 2315
BsuTUI ATCGAT 1 cut(s) 1875
BtgI CCRYGG 1 cut(s) 2310
BtrI CACGTC 1 cut(s) 2534
BtsCI GGATG 6 cut(s) 791, 1099, 1216, 1628, 2218, 2282
BtsI GCAGTG 2 cut(s) 18, 1791
BtsIMutI CAGTG 6 cut(s) 18, 570, 744, 1522, 1791, 1959
Cac8I GCNNGC 2 cut(s) 1318, 1683
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 2 cut(s) 148, 611
ClaI ATCGAT 1 cut(s) 1875
CseI GACGC 1 cut(s) 1647
Csp6I GTAC 6 cut(s) 883, 945, 966, 1786, 2165, 2230
CviQI GTAC 6 cut(s) 883, 945, 966, 1786, 2165, 2230
DinI GGCGCC 1 cut(s) 868
DpnI GATC 5 cut(s) 389, 1059, 1491, 1740, 2036
DpnII GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
EaeI YGGCCR 3 cut(s) 109, 2122, 2313
Eam1104I CTCTTC 1 cut(s) 1374
EarI CTCTTC 1 cut(s) 1374
Eco130I CCWWGG 4 cut(s) 151, 211, 441, 2310
Eco31I GGTCTC 2 cut(s) 46, 161
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 5 cut(s) 516, 581, 992, 2009, 2565
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 2 cut(s) 2216, 2358
EcoRII CCWGG 4 cut(s) 116, 1188, 2269, 2536
EcoT14I CCWWGG 4 cut(s) 151, 211, 441, 2310
EcoT22I ATGCAT 2 cut(s) 791, 1510
EgeI GGCGCC 1 cut(s) 868
EheI GGCGCC 1 cut(s) 868
ErhI CCWWGG 4 cut(s) 151, 211, 441, 2310
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 2384, 2416
FauNDI CATATG 2 cut(s) 130, 1287
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 2281
Fnu4HI GCNGC 9 cut(s) 610, 936, 1119, 1233, 1236, 1314, 1631, 1951, 2484
FokI GGATG 6 cut(s) 798, 1086, 1223, 1635, 2225, 2289
Fsp4HI GCNGC 9 cut(s) 610, 936, 1119, 1233, 1236, 1314, 1631, 1951, 2484
FspBI CTAG 5 cut(s) 932, 1332, 1493, 1869, 2325
GlaI GCGC 1 cut(s) 868
GluI GCNGC 9 cut(s) 610, 936, 1119, 1233, 1236, 1314, 1631, 1951, 2484
GsuI CTGGAG 5 cut(s) 506, 777, 1157, 1245, 2177
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 4 cut(s) 111, 612, 2124, 2315
HapII CCGG 5 cut(s) 430, 1055, 1359, 2032, 2210
HgaI GACGC 1 cut(s) 1647
HhaI GCGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 867
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HindIII AAGCTT 3 cut(s) 304, 547, 1128
HinfI GANTC 7 cut(s) 458, 1589, 1872, 2091, 2159, 2328, 2433
HpaII CCGG 5 cut(s) 430, 1055, 1359, 2032, 2210
HphI GGTGA 7 cut(s) 358, 673, 1031, 1390, 1532, 1784, 2020
Hpy166II GTNNAC 6 cut(s) 641, 883, 1092, 1398, 1450, 2282
Hpy188I TCNGA 6 cut(s) 475, 535, 693, 1011, 1743, 1978
Hpy188III TCNNGA 8 cut(s) 346, 359, 398, 485, 905, 1055, 1895, 2325
Hpy8I GTNNAC 6 cut(s) 641, 883, 1092, 1398, 1450, 2282
HpyCH4III ACNGT 5 cut(s) 124, 748, 887, 1454, 2286
HpyCH4IV ACGT 1 cut(s) 2533
HpyF10VI GCNNNNNNNGC 8 cut(s) 803, 812, 1003, 1127, 1229, 1419, 1655, 1931
HpySE526I ACGT 1 cut(s) 2533
Hsp92I GRCGYC 1 cut(s) 867
HspAI GCGC 1 cut(s) 867
KasI GGCGCC 1 cut(s) 866
Kpn2I TCCGGA 1 cut(s) 1054
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
LmnI GCTCC 3 cut(s) 1138, 1181, 2338
Lsp1109I GCAGC 8 cut(s) 947, 1105, 1219, 1247, 1325, 1642, 1937, 2470
LweI GCATC 5 cut(s) 776, 802, 1130, 1495, 1974
MaeI CTAG 5 cut(s) 932, 1332, 1493, 1869, 2325
MaeII ACGT 1 cut(s) 2533
MaeIII GTNAC 9 cut(s) 124, 413, 459, 905, 1042, 1327, 2051, 2299, 2496
MalI GATC 5 cut(s) 389, 1059, 1491, 1740, 2036
MboI GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
MboII GAAGA 7 cut(s) 157, 334, 667, 1391, 1544, 1823, 2313
MfeI CAATTG 1 cut(s) 2063
MhlI GDGCHC 2 cut(s) 1267, 2343
MlsI TGGCCA 2 cut(s) 111, 2315
MluCI AATT 8 cut(s) 1167, 1991, 2063, 2129, 2216, 2358, 2395, 2443
MluNI TGGCCA 2 cut(s) 111, 2315
Mly113I GGCGCC 1 cut(s) 867
MlyI GAGTC 3 cut(s) 467, 2168, 2322
MmeI TCCRAC 2 cut(s) 654, 2366
Mox20I TGGCCA 2 cut(s) 111, 2315
Mph1103I ATGCAT 2 cut(s) 791, 1510
MroI TCCGGA 1 cut(s) 1054
MroXI GAANNNNTTC 3 cut(s) 78, 329, 480
MscI TGGCCA 2 cut(s) 111, 2315
MslI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 2082, 2204, 2380
Msp20I TGGCCA 2 cut(s) 111, 2315
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 5 cut(s) 430, 1055, 1359, 2032, 2210
MspR9I CCNGG 4 cut(s) 118, 1190, 2271, 2538
MunI CAATTG 1 cut(s) 2063
MvaI CCWGG 4 cut(s) 118, 1190, 2271, 2538
MwoI GCNNNNNNNGC 8 cut(s) 803, 812, 1003, 1127, 1229, 1419, 1655, 1931
NarI GGCGCC 1 cut(s) 867
NcoI CCATGG 1 cut(s) 2310
NdeI CATATG 2 cut(s) 130, 1287
NdeII GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
NlaIV GGNNCC 2 cut(s) 868, 1918
NmuCI GTSAC 6 cut(s) 124, 459, 905, 1042, 2051, 2496
NsiI ATGCAT 2 cut(s) 791, 1510
NspI RCATGY 4 cut(s) 234, 241, 1685, 2120
NspV TTCGAA 1 cut(s) 2452
PaeI GCATGC 1 cut(s) 1685
PciI ACATGT 2 cut(s) 237, 2116
PdmI GAANNNNTTC 3 cut(s) 78, 329, 480
PfeI GAWTC 4 cut(s) 1589, 1872, 2091, 2433
PflMI CCANNNNNTGG 1 cut(s) 633
PkrI GCNGC 9 cut(s) 611, 937, 1120, 1234, 1237, 1315, 1632, 1952, 2485
PleI GAGTC 3 cut(s) 466, 2167, 2322
PluTI GGCGCC 1 cut(s) 870
PpsI GAGTC 3 cut(s) 466, 2167, 2322
PscI ACATGT 2 cut(s) 237, 2116
PshAI GACNNNNGTC 2 cut(s) 153, 1548
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 4 cut(s) 116, 1188, 2269, 2536
PspGI CCWGG 4 cut(s) 116, 1188, 2269, 2536
PspN4I GGNNCC 2 cut(s) 868, 1918
PspPI GGNCC 2 cut(s) 148, 611
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstI CTGCAG 2 cut(s) 1237, 1955
RsaI GTAC 6 cut(s) 884, 946, 967, 1787, 2166, 2231
RsaNI GTAC 6 cut(s) 883, 945, 966, 1786, 2165, 2230
RseI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 2082, 2204, 2380
SatI GCNGC 9 cut(s) 610, 936, 1119, 1233, 1236, 1314, 1631, 1951, 2484
Sau3AI GATC 5 cut(s) 387, 1057, 1489, 1738, 2034
Sau96I GGNCC 2 cut(s) 148, 611
ScaI AGTACT 1 cut(s) 967
SchI GAGTC 3 cut(s) 467, 2168, 2322
ScrFI CCNGG 4 cut(s) 118, 1190, 2271, 2538
SduI GDGCHC 2 cut(s) 1267, 2343
SfaNI GCATC 5 cut(s) 776, 802, 1130, 1495, 1974
SfcI CTRYAG 3 cut(s) 1233, 1653, 1951
SfoI GGCGCC 1 cut(s) 868
SfuI TTCGAA 1 cut(s) 2452
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 2082, 2204, 2380
SmlI CTYRAG 4 cut(s) 301, 398, 407, 1082
SmoI CTYRAG 4 cut(s) 301, 398, 407, 1082
SphI GCATGC 1 cut(s) 1685
Sse9I AATT 8 cut(s) 1167, 1991, 2063, 2129, 2216, 2358, 2395, 2443
SsiI CCGC 2 cut(s) 609, 2059
SspDI GGCGCC 1 cut(s) 866
SspI AATATT 2 cut(s) 1800, 2465
SspMI CTAG 5 cut(s) 932, 1332, 1493, 1869, 2325
StyD4I CCNGG 4 cut(s) 116, 1188, 2269, 2536
StyI CCWWGG 4 cut(s) 151, 211, 441, 2310
TaaI ACNGT 5 cut(s) 124, 748, 887, 1454, 2286
TaiI ACGT 1 cut(s) 2536
TaqI TCGA 5 cut(s) 289, 1124, 1714, 1875, 2452
TaqII GACCGA 1 cut(s) 1560
TasI AATT 8 cut(s) 1167, 1991, 2063, 2129, 2216, 2358, 2395, 2443
TatI WGTACW 5 cut(s) 882, 944, 965, 1785, 2164
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 4 cut(s) 1589, 1872, 2091, 2433
TscAI CASTG 6 cut(s) 18, 570, 751, 1522, 1798, 1966
TseFI GTSAC 6 cut(s) 124, 459, 905, 1042, 2051, 2496
TseI GCWGC 8 cut(s) 935, 1118, 1232, 1235, 1313, 1630, 1950, 2483
Tsp45I GTSAC 6 cut(s) 124, 459, 905, 1042, 2051, 2496
TspGWI ACGGA 1 cut(s) 1319
TspRI CASTG 6 cut(s) 18, 570, 751, 1522, 1798, 1966
Van91I CCANNNNNTGG 1 cut(s) 633
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 7 cut(s) 1167, 1991, 2129, 2216, 2358, 2395, 2443
XbaI TCTAGA 1 cut(s) 2324
XceI RCATGY 4 cut(s) 234, 241, 1685, 2120
XmiI GTMKAC 1 cut(s) 2281
XmnI GAANNNNTTC 3 cut(s) 78, 329, 480
XspI CTAG 5 cut(s) 932, 1332, 1493, 1869, 2325
ZrmI AGTACT 1 cut(s) 967
Zsp2I ATGCAT 2 cut(s) 791, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.