RchiOBHm_Chr4g0394921

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000555
Physical Location & Seq
Reverse (-)
10696950 .. 10709700
12751 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36739

Sequence Viewer

Length: 2472 bp
ATGAAGGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGCGTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCCAGGACTATCACATATGATGGCGTTGATTTGGACCTTGGTCTCATGGTCTTCAATCATGTGATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGTGACATGTCCTTCTCAGTGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCGAAATAGTTTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCTTGAGAGTAACCCAGACATTGATCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTCTGGAGGCTTACCTTATTCCAATGTGTAGTGCAATCTTGTCGTGTCCTTCAGAAGGAGTTATAAGCTTCTCAGCTTTCTCAGTGCTTTCGGTTTGTCGCAATCATCATCTACTTCAGCTATTTGGCGGCCCACAATGGCTTACCATCAAATGGGGTTCACATTGTTATGTGAAGAAGGTGAAAGAAGTGTTGGAGAGTAAAGGGTGTCAGATAAGAACCGGCTGTGAGGTGCATAAAGTTTCCACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTTTTGAGGAAATATACGATGGATGCATTATAGCTATCCAAGCCCCCGATGCCATGAGAATGTTAGGAGAACAGGCAACACCTGATGAACTGAGAATACTTGGCGCCTTCCAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCGAAATCTAGCAGCATGGAGTGCATTAAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGAACCTCGGTGAAACAAGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCTGCATTGAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTATTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGGCTGGAGTGGCTGTAGCACATGGTATGCTTGGAAAAGGCTGTGCCCTATTGGGAAACCCAAAACATATGGCTCTTTCATTGACGGAAACAGCAGCACGCCTATTTGTTACTAGATTTTTTAGACATTATATATCTACCGGATGTTTAGTCTTATTAGAAGAGGGAGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAAATCCATTCTTAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATCAGTGGTGATTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTCTGAGTAATGAACTATTCTCGCTGTTCTTGGGTGAAACGATGATGTACTCCACTGCAATATTTAAGGTCGGAGTCACCAGCTTCCAGTCAGTTTACTACAATAAACACTCATGGAAGAAAACAGAAGATGAGGATTTGAATACTGCACAGCTTAGGAAAATCTCTATTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTAGATATTGGTTGTGGTTGGGGAACCTTTGCTATTGAAGCAGTCAAACAAACAGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAGATTTGCTGAAAAGAAAGTGAAAAATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTGTGACTACCGCCAATTGCCTTCTTACCACAAATATGACAGAATCATATCTTGTGAGATGCTAGAACATGTCGGCCATGAATTTATGGACGAGTTCTTTTCTTGCTGTGAGTCTGTACTGGCAAAAAATGGGCTTTTTATTCTTCAGTTCATATCTATGCCGGATGAATTCTATGATGAGTACCTACGAAGTTCAGGCTTCATGAAGGAATATATATTTCCTGGTGGGTGTCTACCGTCATTAAGTAGGGTAACATCAGCCATGGCCACTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGTATTCATTATTATCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGAGAGACACAGTGAAATACTCGCTCTTGGATTCAATGAAAATTTCATTCGAACATGGGAATATTATTTTGATTATTGTGCTGCTGGTTTCAAGTCACATACACTTGGAGACTACCAGATTGTGTTTTCACGTCCTGGTAATGCTCTCACATTTAACAATCCATATAAGGGTTTCCCTTCAGCATATTGA

Protein Analysis

823

Amino Acids

93.4

Weight (kDa)

5.95

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 5e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 71 2.2e-17 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 273 2e-21 Flavin containing amine oxidoreductase
CMAS PF02353 523 - 796 7e-69 Mycolic acid cyclopropane synthetase
MTS PF05175 580 - 689 5.3e-06 Methyltransferase small domain
Methyltransf_23 PF13489 583 - 698 5.3e-10 Methyltransferase domain
Methyltransf_31 PF13847 586 - 700 1.4e-14 Methyltransferase domain
Methyltransf_25 PF13649 591 - 687 2.2e-17 Methyltransferase domain
Methyltransf_11 PF08241 592 - 691 3.4e-14 Methyltransferase domain
Methyltransf_12 PF08242 592 - 689 7.3e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 633
AccI GTMKAC 1 cut(s) 2161
AciI CCGC 2 cut(s) 609, 1939
AclWI GGATC 1 cut(s) 1922
AcoI YGGCCR 3 cut(s) 109, 2002, 2193
AcsI RAATTY 5 cut(s) 1167, 2009, 2096, 2275, 2323
AcuI CTGAAG 6 cut(s) 516, 581, 992, 1889, 2057, 2445
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 5 cut(s) 884, 967, 1613, 2046, 2111
AfiI CCNNNNNNNGG 3 cut(s) 536, 633, 2450
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 3 cut(s) 230, 237, 1996
AjiI CACGTC 1 cut(s) 2414
AjnI CCWGG 5 cut(s) 102, 116, 1188, 2149, 2416
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 656, 688
Alw21I GWGCWC 1 cut(s) 2223
Alw26I GTCTC 5 cut(s) 46, 161, 221, 2281, 2385
AlwI GGATC 1 cut(s) 1922
AoxI GGCC 4 cut(s) 109, 610, 2002, 2193
ApeKI GCWGC 5 cut(s) 935, 1118, 1313, 1830, 2363
ApoI RAATTY 5 cut(s) 1167, 2009, 2096, 2275, 2323
Asp700I GAANNNNTTC 3 cut(s) 78, 329, 480
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 2 cut(s) 148, 611
AsuHPI GGTGA 7 cut(s) 358, 673, 1031, 1390, 1532, 1610, 1633
AsuII TTCGAA 1 cut(s) 2332
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 1267
BalI TGGCCA 2 cut(s) 111, 2195
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 157, 1544
Bbv12I GWGCWC 1 cut(s) 2223
BbvI GCAGC 5 cut(s) 947, 1105, 1325, 1817, 2350
BccI CCATC 7 cut(s) 128, 187, 635, 755, 776, 1107, 1205
BcgI CGANNNNNNTGC 2 cut(s) 504, 538
BciT130I CCWGG 5 cut(s) 104, 118, 1190, 2151, 2418
BciVI GTATCC 1 cut(s) 192
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 5 cut(s) 46, 161, 221, 2281, 2385
BfaI CTAG 7 cut(s) 932, 1332, 1493, 1749, 1775, 1991, 2205
BfmI CTRYAG 2 cut(s) 1233, 1831
BfoI RGCGCY 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 6 cut(s) 610, 936, 1119, 1314, 1831, 2364
BlsI GCNGC 6 cut(s) 611, 937, 1120, 1315, 1832, 2365
BmcAI AGTACT 1 cut(s) 967
Bme1390I CCNGG 5 cut(s) 104, 118, 1190, 2151, 2418
Bme18I GGWCC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 2414
BmgT120I GGNCC 2 cut(s) 148, 611
BmiI GGNNCC 2 cut(s) 868, 1798
BmrFI CCNGG 5 cut(s) 104, 118, 1190, 2151, 2418
BmsI GCATC 5 cut(s) 776, 802, 1495, 1854, 1977
BoxI GACNNNNGTC 2 cut(s) 153, 1548
BpiI GAAGAC 2 cut(s) 157, 1544
BpmI CTGGAG 4 cut(s) 506, 777, 1157, 1245
Bpu10I CCTNAGC 1 cut(s) 1718
Bpu14I TTCGAA 1 cut(s) 2332
BpuEI CTTGAG 3 cut(s) 286, 419, 428
Bsa29I ATCGAT 1 cut(s) 1755
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 2 cut(s) 46, 161
BsaJI CCNNGG 8 cut(s) 103, 151, 211, 441, 1015, 1189, 1546, 2190
BsaWI WCCGGW 2 cut(s) 1358, 1911
BsaXI ACNNNNNCTCC 2 cut(s) 1218, 1248
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 633, 2450
Bse118I RCCGGY 1 cut(s) 701
Bse1I ACTGG 7 cut(s) 760, 998, 1103, 1472, 1651, 1846, 2052
Bse3DI GCAATG 1 cut(s) 1497
BseBI CCWGG 5 cut(s) 104, 118, 1190, 2151, 2418
BseCI ATCGAT 1 cut(s) 1755
BseDI CCNNGG 8 cut(s) 103, 151, 211, 441, 1015, 1189, 1546, 2190
BseGI GGATG 5 cut(s) 791, 1099, 1216, 1367, 2098
BseLI CCNNNNNNNGG 3 cut(s) 536, 633, 2450
BseMI GCAATG 1 cut(s) 1497
BseMII CTCAG 8 cut(s) 57, 261, 567, 576, 845, 1472, 1559, 1848
BseNI ACTGG 7 cut(s) 760, 998, 1103, 1472, 1651, 1846, 2052
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 5 cut(s) 947, 1105, 1325, 1817, 2350
BsgI GTGCAG 1 cut(s) 1695
BshFI GGCC 4 cut(s) 111, 612, 2004, 2195
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1755
BsiHKAI GWGCWC 1 cut(s) 2223
BsiSI CCGG 4 cut(s) 702, 1359, 1912, 2090
BslI CCNNNNNNNGG 3 cut(s) 536, 633, 2450
BsmAI GTCTC 5 cut(s) 46, 161, 221, 2281, 2385
BsnI GGCC 4 cut(s) 111, 612, 2004, 2195
Bso31I GGTCTC 2 cut(s) 46, 161
Bsp119I TTCGAA 1 cut(s) 2332
Bsp1286I GDGCHC 2 cut(s) 1267, 2223
Bsp143I GATC 5 cut(s) 387, 427, 1057, 1489, 1914
Bsp19I CCATGG 1 cut(s) 2190
BspACI CCGC 2 cut(s) 609, 1939
BspANI GGCC 4 cut(s) 111, 612, 2004, 2195
BspCNI CTCAG 8 cut(s) 56, 260, 566, 575, 846, 1471, 1560, 1849
BspDI ATCGAT 1 cut(s) 1755
BspHI TCATGA 1 cut(s) 2130
BspLI GGNNCC 2 cut(s) 868, 1798
BspMAI CTGCAG 1 cut(s) 1835
BspPI GGATC 1 cut(s) 1922
BspT104I TTCGAA 1 cut(s) 2332
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 2 cut(s) 46, 161
BsrDI GCAATG 1 cut(s) 1497
BsrFI RCCGGY 1 cut(s) 701
BsrI ACTGG 7 cut(s) 760, 998, 1103, 1472, 1651, 1846, 2052
BssAI RCCGGY 1 cut(s) 701
BssECI CCNNGG 8 cut(s) 103, 151, 211, 441, 1015, 1189, 1546, 2190
BssMI GATC 5 cut(s) 387, 427, 1057, 1489, 1914
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 4 cut(s) 151, 211, 441, 2190
Bst2UI CCWGG 5 cut(s) 104, 118, 1190, 2151, 2418
Bst4CI ACNGT 6 cut(s) 748, 887, 1424, 1454, 2166, 2294
Bst6I CTCTTC 1 cut(s) 1374
BstACI GRCGYC 1 cut(s) 867
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 944
BstBI TTCGAA 1 cut(s) 2332
BstC8I GCNNGC 1 cut(s) 1318
BstDSI CCRYGG 1 cut(s) 2190
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 5 cut(s) 791, 1099, 1216, 1367, 2098
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 5 cut(s) 390, 430, 1060, 1492, 1917
BstMAI GTCTC 5 cut(s) 46, 161, 221, 2281, 2385
BstMBI GATC 5 cut(s) 387, 427, 1057, 1489, 1914
BstMWI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1811
BstNI CCWGG 5 cut(s) 104, 118, 1190, 2151, 2418
BstNSI RCATGY 3 cut(s) 234, 241, 2000
BstPAI GACNNNNGTC 2 cut(s) 153, 1548
BstSCI CCNGG 5 cut(s) 102, 116, 1188, 2149, 2416
BstSFI CTRYAG 2 cut(s) 1233, 1831
BstSLI GKGCMC 1 cut(s) 1267
BstV1I GCAGC 5 cut(s) 947, 1105, 1325, 1817, 2350
BstV2I GAAGAC 2 cut(s) 157, 1544
BstXI CCANNNNNNTGG 3 cut(s) 345, 1196, 1211
Bsu15I ATCGAT 1 cut(s) 1755
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 4 cut(s) 111, 612, 2004, 2195
BsuTUI ATCGAT 1 cut(s) 1755
BtgI CCRYGG 1 cut(s) 2190
BtrI CACGTC 1 cut(s) 2414
BtsCI GGATG 5 cut(s) 791, 1099, 1216, 1367, 2098
BtsI GCAGTG 2 cut(s) 18, 1617
BtsIMutI CAGTG 8 cut(s) 18, 255, 570, 744, 1522, 1617, 1839, 2299
Cac8I GCNNGC 1 cut(s) 1318
CciI TCATGA 1 cut(s) 2130
CfoI GCGC 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 701
Cfr13I GGNCC 2 cut(s) 148, 611
ClaI ATCGAT 1 cut(s) 1755
Csp6I GTAC 5 cut(s) 883, 966, 1612, 2045, 2110
CviQI GTAC 5 cut(s) 883, 966, 1612, 2045, 2110
DinI GGCGCC 1 cut(s) 868
DpnI GATC 5 cut(s) 389, 429, 1059, 1491, 1916
DpnII GATC 5 cut(s) 387, 427, 1057, 1489, 1914
EaeI YGGCCR 3 cut(s) 109, 2002, 2193
Eam1104I CTCTTC 1 cut(s) 1374
EarI CTCTTC 1 cut(s) 1374
Eco130I CCWWGG 4 cut(s) 151, 211, 441, 2190
Eco31I GGTCTC 2 cut(s) 46, 161
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 6 cut(s) 516, 581, 992, 1889, 2057, 2445
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 2096
EcoRII CCWGG 5 cut(s) 102, 116, 1188, 2149, 2416
EcoT14I CCWWGG 4 cut(s) 151, 211, 441, 2190
EcoT22I ATGCAT 2 cut(s) 791, 1510
EgeI GGCGCC 1 cut(s) 868
EheI GGCGCC 1 cut(s) 868
ErhI CCWWGG 4 cut(s) 151, 211, 441, 2190
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 2264, 2296
FauNDI CATATG 2 cut(s) 130, 1287
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 2161
Fnu4HI GCNGC 6 cut(s) 610, 936, 1119, 1314, 1831, 2364
FokI GGATG 5 cut(s) 798, 1086, 1223, 1374, 2105
Fsp4HI GCNGC 6 cut(s) 610, 936, 1119, 1314, 1831, 2364
FspBI CTAG 7 cut(s) 932, 1332, 1493, 1749, 1775, 1991, 2205
GlaI GCGC 1 cut(s) 868
GluI GCNGC 6 cut(s) 610, 936, 1119, 1314, 1831, 2364
GsuI CTGGAG 4 cut(s) 506, 777, 1157, 1245
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 4 cut(s) 111, 612, 2004, 2195
HapII CCGG 4 cut(s) 702, 1359, 1912, 2090
HhaI GCGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 867
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 2 cut(s) 1031, 1092
HindII GTYRAC 2 cut(s) 1031, 1092
HindIII AAGCTT 3 cut(s) 304, 547, 1128
HinfI GANTC 7 cut(s) 458, 1638, 1752, 1971, 2039, 2208, 2313
HpaII CCGG 4 cut(s) 702, 1359, 1912, 2090
HphI GGTGA 7 cut(s) 358, 673, 1031, 1390, 1532, 1610, 1633
Hpy166II GTNNAC 8 cut(s) 641, 883, 1031, 1092, 1398, 1450, 1660, 2162
Hpy188I TCNGA 8 cut(s) 432, 475, 535, 693, 1011, 1569, 1637, 1858
Hpy188III TCNNGA 9 cut(s) 346, 359, 398, 485, 905, 1055, 1775, 2131, 2205
Hpy8I GTNNAC 8 cut(s) 641, 883, 1031, 1092, 1398, 1450, 1660, 2162
HpyCH4III ACNGT 6 cut(s) 748, 887, 1424, 1454, 2166, 2294
HpyCH4IV ACGT 1 cut(s) 2413
HpyF10VI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1811
HpySE526I ACGT 1 cut(s) 2413
Hsp92I GRCGYC 1 cut(s) 867
HspAI GCGC 1 cut(s) 867
KasI GGCGCC 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 5 cut(s) 387, 427, 1057, 1489, 1914
LmnI GCTCC 3 cut(s) 1138, 1181, 2218
Lsp1109I GCAGC 5 cut(s) 947, 1105, 1325, 1817, 2350
LweI GCATC 5 cut(s) 776, 802, 1495, 1854, 1977
MaeI CTAG 7 cut(s) 932, 1332, 1493, 1749, 1775, 1991, 2205
MaeII ACGT 1 cut(s) 2413
MalI GATC 5 cut(s) 389, 429, 1059, 1491, 1916
MboI GATC 5 cut(s) 387, 427, 1057, 1489, 1914
MboII GAAGA 9 cut(s) 157, 334, 667, 1391, 1544, 1693, 1703, 2063, 2193
MfeI CAATTG 1 cut(s) 1943
MhlI GDGCHC 2 cut(s) 1267, 2223
MlsI TGGCCA 2 cut(s) 111, 2195
MluCI AATT 6 cut(s) 1167, 1943, 2009, 2096, 2275, 2323
MluNI TGGCCA 2 cut(s) 111, 2195
Mly113I GGCGCC 1 cut(s) 867
MlyI GAGTC 4 cut(s) 467, 1647, 2048, 2202
MmeI TCCRAC 3 cut(s) 654, 1615, 2246
Mox20I TGGCCA 2 cut(s) 111, 2195
Mph1103I ATGCAT 2 cut(s) 791, 1510
MroXI GAANNNNTTC 3 cut(s) 78, 329, 480
MscI TGGCCA 2 cut(s) 111, 2195
MseI TTAA 8 cut(s) 375, 917, 950, 1083, 1443, 1629, 2171, 2436
MslI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 1962, 2084, 2260
Msp20I TGGCCA 2 cut(s) 111, 2195
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 4 cut(s) 702, 1359, 1912, 2090
MspR9I CCNGG 5 cut(s) 104, 118, 1190, 2151, 2418
MunI CAATTG 1 cut(s) 1943
MvaI CCWGG 5 cut(s) 104, 118, 1190, 2151, 2418
MwoI GCNNNNNNNGC 7 cut(s) 803, 812, 944, 1003, 1127, 1229, 1811
NarI GGCGCC 1 cut(s) 867
NcoI CCATGG 1 cut(s) 2190
NdeI CATATG 2 cut(s) 130, 1287
NdeII GATC 5 cut(s) 387, 427, 1057, 1489, 1914
NlaIV GGNNCC 2 cut(s) 868, 1798
NmuCI GTSAC 7 cut(s) 233, 459, 905, 1042, 1639, 1931, 2376
NsiI ATGCAT 2 cut(s) 791, 1510
NspI RCATGY 3 cut(s) 234, 241, 2000
NspV TTCGAA 1 cut(s) 2332
PagI TCATGA 1 cut(s) 2130
PciI ACATGT 3 cut(s) 230, 237, 1996
PdmI GAANNNNTTC 3 cut(s) 78, 329, 480
PfeI GAWTC 3 cut(s) 1752, 1971, 2313
PflMI CCANNNNNTGG 1 cut(s) 633
PkrI GCNGC 6 cut(s) 611, 937, 1120, 1315, 1832, 2365
PleI GAGTC 4 cut(s) 466, 1646, 2047, 2202
PluTI GGCGCC 1 cut(s) 870
PpsI GAGTC 4 cut(s) 466, 1646, 2047, 2202
PscI ACATGT 3 cut(s) 230, 237, 1996
PshAI GACNNNNGTC 2 cut(s) 153, 1548
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 5 cut(s) 102, 116, 1188, 2149, 2416
PspGI CCWGG 5 cut(s) 102, 116, 1188, 2149, 2416
PspN4I GGNNCC 2 cut(s) 868, 1798
PspPI GGNCC 2 cut(s) 148, 611
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstI CTGCAG 1 cut(s) 1835
RsaI GTAC 5 cut(s) 884, 967, 1613, 2046, 2111
RsaNI GTAC 5 cut(s) 883, 966, 1612, 2045, 2110
RseI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 1962, 2084, 2260
SaqAI TTAA 8 cut(s) 375, 917, 950, 1083, 1443, 1629, 2171, 2436
SatI GCNGC 6 cut(s) 610, 936, 1119, 1314, 1831, 2364
Sau3AI GATC 5 cut(s) 387, 427, 1057, 1489, 1914
Sau96I GGNCC 2 cut(s) 148, 611
ScaI AGTACT 1 cut(s) 967
SchI GAGTC 4 cut(s) 467, 1647, 2048, 2202
ScrFI CCNGG 5 cut(s) 104, 118, 1190, 2151, 2418
SduI GDGCHC 2 cut(s) 1267, 2223
SfaNI GCATC 5 cut(s) 776, 802, 1495, 1854, 1977
SfcI CTRYAG 2 cut(s) 1233, 1831
SfoI GGCGCC 1 cut(s) 868
SfuI TTCGAA 1 cut(s) 2332
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 6 cut(s) 648, 821, 1209, 1962, 2084, 2260
SmlI CTYRAG 4 cut(s) 301, 398, 407, 1082
SmoI CTYRAG 4 cut(s) 301, 398, 407, 1082
Sse9I AATT 6 cut(s) 1167, 1943, 2009, 2096, 2275, 2323
SsiI CCGC 2 cut(s) 609, 1939
SspDI GGCGCC 1 cut(s) 866
SspI AATATT 2 cut(s) 1626, 2345
SspMI CTAG 7 cut(s) 932, 1332, 1493, 1749, 1775, 1991, 2205
StyD4I CCNGG 5 cut(s) 102, 116, 1188, 2149, 2416
StyI CCWWGG 4 cut(s) 151, 211, 441, 2190
TaaI ACNGT 6 cut(s) 748, 887, 1424, 1454, 2166, 2294
TaiI ACGT 1 cut(s) 2416
TaqI TCGA 3 cut(s) 289, 1755, 2332
TaqII GACCGA 1 cut(s) 1560
TasI AATT 6 cut(s) 1167, 1943, 2009, 2096, 2275, 2323
TatI WGTACW 4 cut(s) 882, 965, 1611, 2044
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 3 cut(s) 1752, 1971, 2313
Tru1I TTAA 8 cut(s) 375, 917, 950, 1083, 1443, 1629, 2171, 2436
Tru9I TTAA 8 cut(s) 375, 917, 950, 1083, 1443, 1629, 2171, 2436
TscAI CASTG 8 cut(s) 18, 255, 570, 751, 1522, 1624, 1846, 2299
TseFI GTSAC 7 cut(s) 233, 459, 905, 1042, 1639, 1931, 2376
TseI GCWGC 5 cut(s) 935, 1118, 1313, 1830, 2363
Tsp45I GTSAC 7 cut(s) 233, 459, 905, 1042, 1639, 1931, 2376
TspGWI ACGGA 1 cut(s) 1319
TspRI CASTG 8 cut(s) 18, 255, 570, 751, 1522, 1624, 1846, 2299
Van91I CCANNNNNTGG 1 cut(s) 633
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 5 cut(s) 1167, 2009, 2096, 2275, 2323
XbaI TCTAGA 2 cut(s) 1774, 2204
XceI RCATGY 3 cut(s) 234, 241, 2000
XmiI GTMKAC 1 cut(s) 2161
XmnI GAANNNNTTC 3 cut(s) 78, 329, 480
XspI CTAG 7 cut(s) 932, 1332, 1493, 1749, 1775, 1991, 2205
ZrmI AGTACT 1 cut(s) 967
Zsp2I ATGCAT 2 cut(s) 791, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.