RchiOBHm_Chr1g0362281

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000555
Physical Location & Seq
Forward (+)
53960315 .. 53968818
8504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58711

Sequence Viewer

Length: 981 bp
ATGGCTGCAGCAACTGGTGTGCTTGGAAAAGGTTGTGCGCTCTTGGAGAAGGTCGCACGCCTATATGTTACTAGATTTCTGAGACAGTATATCTCTACAGGATGTTTAGTCTTATTAGAAGAAAGAATGGTGCAAACCTTTGAGGGAAGCAATAAAGGCTGTTCTTTGAAATCTGTTATTAAAGTTCATAACCCCGTTCAATTTTACTGGAAGGAAGCTTGTTTAGCCCCAGCAAGATATCTCTTTCATCACGTTTCAAGGCAAAACTCTCTTACAGAGGCTCGAAGGAACATCTCTCACCATTATGATCTGAAAGAAGATGAAGAGTTGAAGACTGCACAGCTAAGGAAAATCAGTATTCTCATTGAAAAGGCTAGAATTACTAAGAACCATGAAGTCCTAGAGTTGGGCTGTGGTTGGGGAGGCTTCGCCATTGAAGTCGTCAAGCAAACCGGTTGTAGATACACTGGCATAACTTTGTCGAAGGAACAAGTGCAATTTGCTGAAAAGAAAGTGAATGATGCTGGCCTTCACATGCTAGAACATGTCGGCCATGAATTTATGGAGGAATTCTTTTCTTGTTGTGACTCTATGCTAGCAGAAAATGGGCTTCTTATTCTACAGGGAAGTTCAGACTTCATAAAGGAGTATATTTTTCCGGGTGGATTGTTACCTTCATTAAGTAGGGTAACATCAGCTATGGTTGCTTCTTCCAGGTTCAGTGTGCAGCACTTGGAAAACATTGGAATTCATTATTACCAAACACTAGGATATTGGAGAAAAACATTCTCGGAGAGACATAGTGAAATACTAGCTCTTGGATTCGATGAAAAATTCATTCGGACATGGGAGTATTATTTTGATTATTGTGCGGCAGGATTCAACTCTTGTACTCTTGGAAATTACCAGATTGTGTTTTCACGTCCCGGTAATGTTCTTGAATTCAGCGATCTATACAAGGGTTTCCCTTCAGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

326

Amino Acids

37.13

Weight (kDa)

7.59

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 105 - 177 1.5e-15 Mycolic acid cyclopropane synthetase
Methyltransf_25 PF13649 133 - 178 3.4e-07 Methyltransferase domain
CMAS PF02353 177 - 299 1.9e-24 Mycolic acid cyclopropane synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 872
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 5 cut(s) 557, 569, 747, 833, 941
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 1 cut(s) 892
AfiI CCNNNNNNNGG 1 cut(s) 406
AflIII ACRYGT 1 cut(s) 544
AgeI ACCGGT 1 cut(s) 452
AgsI TTSAA 8 cut(s) 169, 200, 258, 331, 368, 437, 883, 941
AjiI CACGTC 1 cut(s) 923
AjnI CCWGG 1 cut(s) 713
AluBI AGCT 4 cut(s) 218, 343, 698, 815
AluI AGCT 4 cut(s) 218, 343, 698, 815
Alw26I GTCTC 2 cut(s) 76, 790
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 2 cut(s) 526, 550
ApeKI GCWGC 3 cut(s) 5, 8, 727
ApoI RAATTY 5 cut(s) 557, 569, 747, 833, 941
AsiGI ACCGGT 1 cut(s) 452
AspLEI GCGC 1 cut(s) 40
AsuC2I CCSGG 2 cut(s) 660, 927
AsuHPI GGTGA 1 cut(s) 290
AsuNHI GCTAGC 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 338
BbvI GCAGC 2 cut(s) 20, 739
BciT130I CCWGG 1 cut(s) 715
BcnI CCSGG 2 cut(s) 660, 927
BcoDI GTCTC 2 cut(s) 76, 790
BfaI CTAG 7 cut(s) 72, 375, 401, 539, 596, 767, 812
BfmI CTRYAG 3 cut(s) 6, 96, 620
BisI GCNGC 4 cut(s) 6, 9, 728, 873
BlsI GCNGC 4 cut(s) 7, 10, 729, 874
Bme1390I CCNGG 3 cut(s) 660, 715, 927
BmgBI CACGTC 1 cut(s) 923
BmrFI CCNGG 3 cut(s) 660, 715, 927
BmsI GCATC 1 cut(s) 511
BmtI GCTAGC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 344
BpuMI CCSGG 2 cut(s) 660, 927
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 3 cut(s) 19, 212, 472
BseBI CCWGG 1 cut(s) 715
BseGI GGATG 1 cut(s) 107
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 3 cut(s) 19, 212, 472
BseXI GCAGC 2 cut(s) 20, 739
BseYI CCCAGC 1 cut(s) 229
BsgI GTGCAG 2 cut(s) 321, 746
BshFI GGCC 2 cut(s) 528, 552
BshTI ACCGGT 1 cut(s) 452
BsiSI CCGG 3 cut(s) 453, 659, 927
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 2 cut(s) 76, 790
BsmFI GGGAC 1 cut(s) 909
BsnI GGCC 2 cut(s) 528, 552
Bsp143I GATC 2 cut(s) 307, 949
BspACI CCGC 1 cut(s) 872
BspANI GGCC 2 cut(s) 528, 552
BspCNI CTCAG 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 599
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 3 cut(s) 19, 212, 472
BssAI RCCGGY 1 cut(s) 452
BssMI GATC 2 cut(s) 307, 949
Bst2UI CCWGG 1 cut(s) 715
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 58, 526, 597
BstDEI CTNAG 3 cut(s) 80, 344, 384
BstF5I GGATG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 310, 952
BstMAI GTCTC 2 cut(s) 76, 790
BstMBI GATC 2 cut(s) 307, 949
BstMWI GCNNNNNNNGC 3 cut(s) 156, 224, 704
BstNI CCWGG 1 cut(s) 715
BstNSI RCATGY 2 cut(s) 538, 548
BstSCI CCNGG 3 cut(s) 658, 713, 925
BstSFI CTRYAG 3 cut(s) 6, 96, 620
BstV1I GCAGC 2 cut(s) 20, 739
BstV2I GAAGAC 1 cut(s) 338
BsuRI GGCC 2 cut(s) 528, 552
BtrI CACGTC 1 cut(s) 923
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 2 cut(s) 465, 727
Cac8I GCNNGC 3 cut(s) 58, 526, 597
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 452
Csp6I GTAC 1 cut(s) 891
CspAI ACCGGT 1 cut(s) 452
CviAII CATG 5 cut(s) 392, 535, 545, 554, 846
CviQI GTAC 1 cut(s) 891
DdeI CTNAG 3 cut(s) 80, 344, 384
DpnI GATC 2 cut(s) 309, 951
DpnII GATC 2 cut(s) 307, 949
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 954
EcoRI GAATTC 3 cut(s) 569, 747, 941
EcoRII CCWGG 1 cut(s) 713
EcoRV GATATC 1 cut(s) 239
FaeI CATG 5 cut(s) 395, 538, 548, 557, 849
FaqI GGGAC 1 cut(s) 909
FatI CATG 5 cut(s) 391, 534, 544, 553, 845
Fnu4HI GCNGC 4 cut(s) 6, 9, 728, 873
FokI GGATG 1 cut(s) 114
Fsp4HI GCNGC 4 cut(s) 6, 9, 728, 873
FspBI CTAG 7 cut(s) 72, 375, 401, 539, 596, 767, 812
GlaI GCGC 1 cut(s) 39
GluI GCNGC 4 cut(s) 6, 9, 728, 873
GsaI CCCAGC 1 cut(s) 233
HaeIII GGCC 2 cut(s) 528, 552
HapII CCGG 3 cut(s) 453, 659, 927
HhaI GCGC 1 cut(s) 40
Hin1II CATG 5 cut(s) 395, 538, 548, 557, 849
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 3 cut(s) 587, 822, 879
HpaII CCGG 3 cut(s) 453, 659, 927
HphI GGTGA 1 cut(s) 290
Hpy188I TCNGA 5 cut(s) 81, 312, 634, 793, 843
Hpy188III TCNNGA 1 cut(s) 938
HpyAV CCTTC 7 cut(s) 43, 205, 279, 478, 539, 684, 978
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 2 cut(s) 252, 922
HpyCH4V TGCA 5 cut(s) 8, 133, 338, 496, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 224, 704
HpyF3I CTNAG 3 cut(s) 80, 344, 384
HpySE526I ACGT 2 cut(s) 252, 922
Hsp92II CATG 5 cut(s) 395, 538, 548, 557, 849
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 307, 949
Lsp1109I GCAGC 2 cut(s) 20, 739
LweI GCATC 1 cut(s) 511
MaeI CTAG 7 cut(s) 72, 375, 401, 539, 596, 767, 812
MaeII ACGT 2 cut(s) 252, 922
MaeIII GTNAC 4 cut(s) 67, 584, 669, 688
MalI GATC 2 cut(s) 309, 951
MboI GATC 2 cut(s) 307, 949
MboII GAAGA 5 cut(s) 131, 329, 335, 343, 702
MluCI AATT 9 cut(s) 200, 378, 497, 557, 569, 747, 833, 901, 941
MlyI GAGTC 1 cut(s) 581
MnlI CCTC 4 cut(s) 136, 271, 416, 559
MseI TTAA 2 cut(s) 180, 680
MslI CAYNNNNRTG 1 cut(s) 303
MspI CCGG 3 cut(s) 453, 659, 927
MspR9I CCNGG 3 cut(s) 660, 715, 927
MvaI CCWGG 1 cut(s) 715
MwoI GCNNNNNNNGC 3 cut(s) 156, 224, 704
NciI CCSGG 2 cut(s) 660, 927
NdeII GATC 2 cut(s) 307, 949
NheI GCTAGC 1 cut(s) 595
NlaIII CATG 5 cut(s) 395, 538, 548, 557, 849
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 2 cut(s) 538, 548
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 2 cut(s) 822, 879
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 4 cut(s) 7, 10, 729, 874
PleI GAGTC 1 cut(s) 581
PpsI GAGTC 1 cut(s) 581
PscI ACATGT 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 713
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 713
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 14
RsaI GTAC 1 cut(s) 892
RsaNI GTAC 1 cut(s) 891
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 180, 680
SatI GCNGC 4 cut(s) 6, 9, 728, 873
Sau3AI GATC 2 cut(s) 307, 949
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 3 cut(s) 660, 715, 927
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 3 cut(s) 6, 96, 620
SmiMI CAYNNNNRTG 1 cut(s) 303
Sse9I AATT 9 cut(s) 200, 378, 497, 557, 569, 747, 833, 901, 941
SsiI CCGC 1 cut(s) 872
SspMI CTAG 7 cut(s) 72, 375, 401, 539, 596, 767, 812
StyD4I CCNGG 3 cut(s) 658, 713, 925
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 2 cut(s) 255, 925
TaqI TCGA 3 cut(s) 283, 482, 825
TasI AATT 9 cut(s) 200, 378, 497, 557, 569, 747, 833, 901, 941
TatI WGTACW 1 cut(s) 890
TauI GCSGC 1 cut(s) 875
TfiI GAWTC 2 cut(s) 822, 879
Tru1I TTAA 2 cut(s) 180, 680
Tru9I TTAA 2 cut(s) 180, 680
TscAI CASTG 2 cut(s) 472, 727
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 3 cut(s) 5, 8, 727
Tsp45I GTSAC 1 cut(s) 584
TspRI CASTG 2 cut(s) 472, 727
XapI RAATTY 5 cut(s) 557, 569, 747, 833, 941
XceI RCATGY 2 cut(s) 538, 548
XspI CTAG 7 cut(s) 72, 375, 401, 539, 596, 767, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.