Rw1G027140

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000555
Physical Location & Seq
Forward (+)
54506840 .. 54518334
11495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027140.1

Sequence Viewer

Length: 813 bp
ATGATGTATTCCGCTGCTGTATTTAAGAAAGAAGATGAAGAGTTGAAGACTGCACAGCTAAGGAAAATCAGTATTCTCATTGAAAAGGCTAGAATTACTAAGCACCATGAAGTCCTAGAGTTGGGCTGTGGTTGGGGAGGCTTCGCCATTGAAGTCGTCAAGCAAACCGGTTGTAGATACACTGGCATAACTTTGTCGAAGGAACAAGTGCAATTTGCTGAAAAGAAAGTGAATGATGCTGGCCTTCAGGAAAGCATCAAAATTATGCTGCGTGACTACCGCGAATTGCCTCAGAACTACAAATATGACAGAATTATTTGTTGTGAGATGCTAGAACATGTCGGCCATGAATTTATGGAGGAATTCTTTTCTTGTTGTGACTCTATGCTAGCAGAAAATGGGCTTCTTATTCTACAGTTCACATTCTTTCCGGATGAGCGTTACGATGAATATAGGCGAAGTTCAGACTTCATAAAGGAGTATATTTTTCCGGGTGGATTGTTACCTTCATTAAGTAGGGTAACATCAGCTATGGTTGCTTCTTCCAGGTTCAGTGTGCAGCACTTGGAAAACATTGGAATTCATTATTACCAAACACTAAGATATTGGAGAAAAACATTCTCGGAGAAACATAGTGAAATACTAGCTCTTGGATTCGATGAAAAATTCATTCGGACATGGGAGTATTATTTTGATTATTGTGCGGCAGGATTCAACTCTTGTACTCTTGGAAATTACCAGATTGTGTTTTCACGTCCCGGTAATGTTCTTGAATTCAGCGATCTATACAAGGGTTTCCCTTCAGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

270

Amino Acids

31.57

Weight (kDa)

5.87

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 1 - 243 2.4e-66 Mycolic acid cyclopropane synthetase
MTS PF05175 25 - 138 1.7e-07 Methyltransferase small domain
Methyltransf_23 PF13489 32 - 140 5e-11 Methyltransferase domain
Methyltransf_31 PF13847 33 - 134 1.5e-09 Methyltransferase domain
Methyltransf_25 PF13649 38 - 134 1.1e-15 Methyltransferase domain
Methyltransf_11 PF08241 39 - 138 5.9e-13 Methyltransferase domain
Methyltransf_12 PF08242 39 - 136 1.6e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 3 cut(s) 12, 280, 704
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 5 cut(s) 350, 362, 579, 665, 773
AcuI CTGAAG 2 cut(s) 230, 786
AfaI GTAC 1 cut(s) 724
AfiI CCNNNNNNNGG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 337
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 5 cut(s) 46, 83, 152, 715, 773
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 1 cut(s) 545
AluBI AGCT 3 cut(s) 58, 530, 647
AluI AGCT 3 cut(s) 58, 530, 647
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 2 cut(s) 241, 343
ApeKI GCWGC 3 cut(s) 14, 268, 559
ApoI RAATTY 5 cut(s) 350, 362, 579, 665, 773
AsiGI ACCGGT 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 492, 759
AsuNHI GCTAGC 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 255, 571
BciT130I CCWGG 1 cut(s) 547
BcnI CCSGG 2 cut(s) 492, 759
BfaI CTAG 5 cut(s) 90, 116, 332, 389, 644
BfmI CTRYAG 1 cut(s) 413
BisI GCNGC 4 cut(s) 15, 269, 560, 705
BlsI GCNGC 4 cut(s) 16, 270, 561, 706
Bme1390I CCNGG 3 cut(s) 492, 547, 759
BmgBI CACGTC 1 cut(s) 755
BmrFI CCNGG 3 cut(s) 492, 547, 759
BmsI GCATC 3 cut(s) 226, 264, 318
BmtI GCTAGC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 59
BpuMI CCSGG 2 cut(s) 492, 759
BsaWI WCCGGW 2 cut(s) 167, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 187
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 547
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 2 cut(s) 255, 571
BsgI GTGCAG 2 cut(s) 36, 578
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 243, 345
BshTI ACCGGT 1 cut(s) 167
BsiSI CCGG 4 cut(s) 168, 431, 491, 759
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 2 cut(s) 243, 345
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 1 cut(s) 781
BspACI CCGC 3 cut(s) 12, 280, 704
BspANI GGCC 2 cut(s) 243, 345
BspCNI CTCAG 1 cut(s) 304
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 1 cut(s) 282
BspOI GCTAGC 1 cut(s) 392
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 167
BssMI GATC 1 cut(s) 781
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 1 cut(s) 417
Bst6I CTCTTC 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 241, 390
BstDEI CTNAG 4 cut(s) 59, 99, 291, 599
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 1 cut(s) 784
BstMBI GATC 1 cut(s) 781
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 3 cut(s) 490, 545, 757
BstSFI CTRYAG 1 cut(s) 413
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 255, 571
BstV2I GAAGAC 1 cut(s) 53
BsuRI GGCC 2 cut(s) 243, 345
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 180, 559
Cac8I GCNNGC 2 cut(s) 241, 390
Cfr10I RCCGGY 1 cut(s) 167
Csp6I GTAC 1 cut(s) 723
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 4 cut(s) 107, 338, 347, 678
CviJI RGCY 9 cut(s) 58, 89, 126, 141, 243, 345, 403, 530, 647
CviKI_1 RGCY 9 cut(s) 58, 89, 126, 141, 243, 345, 403, 530, 647
CviQI GTAC 1 cut(s) 723
DdeI CTNAG 4 cut(s) 59, 99, 291, 599
DpnI GATC 1 cut(s) 783
DpnII GATC 1 cut(s) 781
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco57I CTGAAG 2 cut(s) 230, 786
EcoRI GAATTC 3 cut(s) 362, 579, 773
EcoRII CCWGG 1 cut(s) 545
FaeI CATG 4 cut(s) 110, 341, 350, 681
FaqI GGGAC 1 cut(s) 741
FatI CATG 4 cut(s) 106, 337, 346, 677
Fnu4HI GCNGC 4 cut(s) 15, 269, 560, 705
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 4 cut(s) 15, 269, 560, 705
FspBI CTAG 5 cut(s) 90, 116, 332, 389, 644
GluI GCNGC 4 cut(s) 15, 269, 560, 705
HaeIII GGCC 2 cut(s) 243, 345
HapII CCGG 4 cut(s) 168, 431, 491, 759
Hin1II CATG 4 cut(s) 110, 341, 350, 681
HinfI GANTC 3 cut(s) 380, 654, 711
HpaII CCGG 4 cut(s) 168, 431, 491, 759
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 4 cut(s) 294, 466, 625, 675
Hpy188III TCNNGA 3 cut(s) 248, 431, 770
Hpy8I GTNNAC 1 cut(s) 420
HpyAV CCTTC 4 cut(s) 193, 254, 516, 810
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 1 cut(s) 754
HpyCH4V TGCA 3 cut(s) 53, 211, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 4 cut(s) 59, 99, 291, 599
HpySE526I ACGT 1 cut(s) 754
Hsp92II CATG 4 cut(s) 110, 341, 350, 681
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 1 cut(s) 781
Lsp1109I GCAGC 2 cut(s) 255, 571
LweI GCATC 3 cut(s) 226, 264, 318
MaeI CTAG 5 cut(s) 90, 116, 332, 389, 644
MaeII ACGT 1 cut(s) 754
MaeIII GTNAC 5 cut(s) 272, 377, 440, 501, 520
MalI GATC 1 cut(s) 783
MboI GATC 1 cut(s) 781
MboII GAAGA 4 cut(s) 44, 50, 58, 534
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 3 cut(s) 131, 300, 352
MroI TCCGGA 1 cut(s) 430
MseI TTAA 2 cut(s) 24, 512
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 4 cut(s) 168, 431, 491, 759
MspR9I CCNGG 3 cut(s) 492, 547, 759
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 2 cut(s) 492, 759
NdeII GATC 1 cut(s) 781
NheI GCTAGC 1 cut(s) 388
NlaIII CATG 4 cut(s) 110, 341, 350, 681
NmuCI GTSAC 2 cut(s) 272, 377
NspI RCATGY 1 cut(s) 341
PciI ACATGT 1 cut(s) 337
PfeI GAWTC 2 cut(s) 654, 711
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 4 cut(s) 16, 270, 561, 706
PleI GAGTC 1 cut(s) 374
PpsI GAGTC 1 cut(s) 374
PscI ACATGT 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
RsaI GTAC 1 cut(s) 724
RsaNI GTAC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 24, 512
SatI GCNGC 4 cut(s) 15, 269, 560, 705
Sau3AI GATC 1 cut(s) 781
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 3 cut(s) 492, 547, 759
SetI ASST 6 cut(s) 60, 508, 532, 551, 649, 757
SfaNI GCATC 3 cut(s) 226, 264, 318
SfcI CTRYAG 1 cut(s) 413
SsiI CCGC 3 cut(s) 12, 280, 704
SspMI CTAG 5 cut(s) 90, 116, 332, 389, 644
StyD4I CCNGG 3 cut(s) 490, 545, 757
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 1 cut(s) 757
TaqI TCGA 2 cut(s) 197, 657
TatI WGTACW 1 cut(s) 722
TauI GCSGC 1 cut(s) 707
TfiI GAWTC 2 cut(s) 654, 711
Tru1I TTAA 2 cut(s) 24, 512
Tru9I TTAA 2 cut(s) 24, 512
TscAI CASTG 2 cut(s) 187, 559
TseFI GTSAC 2 cut(s) 272, 377
TseI GCWGC 3 cut(s) 14, 268, 559
Tsp45I GTSAC 2 cut(s) 272, 377
TspDTI ATGAA 9 cut(s) 51, 123, 363, 460, 462, 498, 572, 658, 675
TspRI CASTG 2 cut(s) 187, 559
XapI RAATTY 5 cut(s) 350, 362, 579, 665, 773
XceI RCATGY 1 cut(s) 341
XspI CTAG 5 cut(s) 90, 116, 332, 389, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.