Rh4CG060200

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000555
Physical Location & Seq
Reverse (-)
11559126 .. 11567180
8055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG060200.1

Sequence Viewer

Length: 2604 bp
ATGAAGGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGCCTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCCAGGACTGTCACATATGATGGTGTTGATTTGGACCTTGGTCTCATGGTCTTCAATCATGTTATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGCGACATGTCCTTCTCAGTGAGTTTAGATAAAGGAAGGGGCTGTGAATGGGGTAGTCAAAATAGTCTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCATGAGAGTAACCCAGACATTGACCGGAATGAAACCTTGGGGCAGTTTATTGAGTTACATCACTACTCTGAACTATTTCTGAAGGCTTACCTTATTCCAATATGTAGTTCAATCTTGTCGTGTCCTTCAGAAGGAGTTATAAGCTTCTCTGCTTTCTCAGTGCTTTCGGTTTGTCGCAATCATCATCTACTTCAGTTTATCTTGCAGCTATTTGGCGGCCCACAATGGCTTACCATCAAATGGGGTTCACACTGTTATGTGAAGAAGGTGAAAGAAGTGTTGGAGAGTAAAGGGTGTCAGATAAGAACCGACTGTGAGGTGCATAAAGTTTCCACAACTGATGAGGGTTGCTCTGTTTTTACTGGAGATGGTTTTGAGGAAATATACAATGGATGCATTATAGCTATCCAAGCCCCCGATGCCATGAGAATATTAGGAGAACAGGCAACACCTGATGAACTGAGAATACTTGGCGCCTTCCAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCTAAATCTAGCAGCATGGAGTGCATTAAACTATCTTGGAAGTACTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGAACCTCAGTGAAACAAGTCAACCATATCTTGTGACACTCAATCCAGAGCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCTGCATCGAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTATGGCTTGCATGAGGATGGGCTAAAGGCTGGAGTGGCTGCAGCACATGGTATGCTTGGAAAAGGTTGTGCCCTGTTGGGAAACCCAAAACATATGGCTCTTTCATTGACGGAAACAGCAGCACGCCTATTTGTTACTAGATTTTTTAGACATTATATATCTACCGGATGTTTAGTCTTATTAGAAGAGGGAGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAAATCCATTCTTAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATCAGTGGTGATTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTCATTGCCAACAGAGATTCTAATGCCTTAAAATCAAACAAGAAAAGGGGATGGTGGCAGCCTTTGTTTTTCACAGCCACTATAGCGTCAGCCAAATGTGTCTTTCAGCATGCTTTAAGGAAAAATAGTCTTACACAGGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCGCTGTTCTTGGGTGAAACGATGATGTACTCCACTGCAATATTTAAGACAGAAGATGAGGATTTGAATACTGCACAGCTTAGGAAAATTTCTATTCTCATTGATAAGGCTAGAATTGATGAGAACCATGAGGTTCTAGATATTGGTTGTGGCTGGGGAACCTTTGCTATTGAAGCAGTCAAACAAACGGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAAATGCTGGACTTCAGCACCGGATCAAGTTCCTTCTGTTTGACTACCGCCAATTGCCTTCTTACCACAAATATGACAGAATCATATCTTGTGAGATGCTAGAACATGTCGGCCATGAATTTATGGACGAGTTCTTTTCTTGCTGTGAGTCTGTACTGGCAAAAAATGGGCTTTTTATTCTCCAGTTCATATCTATGCCGGATGAATTCTATGATGAGTACCTACGAAGTTCAGGCTTCATAAAGGAATATATATTTCCTGGTGGATGTCTACCGTCATTAAGTAGGGTAACATCAGCCATGGCCACTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGAATTCATTATTATCAAACACTAAGATGTTGGAGAAAGAATTTCTTAGAGAGACAGAGTGAAATACTCGCTCTTGGATTCAATGAAAATTTCATTCGAACATGGGAATATTATTTTGATTATTGTGCTGCTGGTTTCAAGTCACATACACTTGGAGACTACCAGATTGTGTTTTCACGTCCTGGTAATGCTCTCACATTCAACAATCCATATAAGGGTTTCCCTTCAGCATATTGA

Protein Analysis

867

Amino Acids

98.41

Weight (kDa)

6.27

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 1.7e-07 FAD dependent oxidoreductase
FAD_binding_2 PF00890 3 - 39 5.4e-06 FAD binding domain
NAD_binding_8 PF13450 5 - 71 3.8e-17 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 279 1.9e-18 Flavin containing amine oxidoreductase
CMAS PF02353 572 - 840 1.3e-79 Mycolic acid cyclopropane synthetase
MTS PF05175 623 - 733 2.7e-06 Methyltransferase small domain
Methyltransf_23 PF13489 627 - 741 7.1e-10 Methyltransferase domain
Methyltransf_31 PF13847 630 - 743 1.3e-13 Methyltransferase domain
Methyltransf_25 PF13649 635 - 731 1.2e-16 Methyltransferase domain
Methyltransf_11 PF08241 636 - 735 4.7e-14 Methyltransferase domain
Methyltransf_12 PF08242 636 - 733 7.7e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 878
AccB7I CCANNNNNTGG 1 cut(s) 645
AccI GTMKAC 1 cut(s) 2293
AciI CCGC 2 cut(s) 621, 2071
AclWI GGATC 1 cut(s) 2054
AcoI YGGCCR 3 cut(s) 109, 2134, 2325
AcsI RAATTY 8 cut(s) 1179, 1857, 2003, 2141, 2228, 2370, 2407, 2455
AcuI CTGAAG 6 cut(s) 506, 516, 581, 1004, 2021, 2577
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 5 cut(s) 896, 979, 1799, 2178, 2243
AfiI CCNNNNNNNGG 4 cut(s) 76, 536, 645, 2582
AflII CTTAAG 1 cut(s) 1094
AflIII ACRYGT 2 cut(s) 237, 2128
AjiI CACGTC 1 cut(s) 2546
AjnI CCWGG 5 cut(s) 102, 116, 1200, 2281, 2548
AjuI GAANNNNNNNTTGG 6 cut(s) 425, 457, 496, 528, 668, 700
AluBI AGCT 8 cut(s) 306, 549, 613, 809, 1018, 1142, 1153, 1849
AluI AGCT 8 cut(s) 306, 549, 613, 809, 1018, 1142, 1153, 1849
Alw21I GWGCWC 1 cut(s) 2355
Alw26I GTCTC 6 cut(s) 46, 161, 221, 302, 2413, 2517
AlwI GGATC 1 cut(s) 2054
AoxI GGCC 5 cut(s) 67, 109, 622, 2134, 2325
ApeKI GCWGC 9 cut(s) 610, 947, 1130, 1244, 1247, 1325, 1642, 1962, 2495
ApoI RAATTY 8 cut(s) 1179, 1857, 2003, 2141, 2228, 2370, 2407, 2455
Asp700I GAANNNNTTC 3 cut(s) 78, 329, 480
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 2 cut(s) 148, 623
AsuHPI GGTGA 5 cut(s) 358, 685, 1402, 1544, 1796
AsuII TTCGAA 1 cut(s) 2464
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 1279
BalI TGGCCA 2 cut(s) 111, 2327
BanI GGYRCC 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 157, 1556
Bbv12I GWGCWC 1 cut(s) 2355
BbvI GCAGC 9 cut(s) 622, 959, 1117, 1231, 1259, 1337, 1654, 1949, 2482
BccI CCATC 7 cut(s) 128, 187, 647, 767, 1119, 1217, 1629
BciT130I CCWGG 5 cut(s) 104, 118, 1202, 2283, 2550
BciVI GTATCC 1 cut(s) 192
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 6 cut(s) 46, 161, 221, 302, 2413, 2517
BfaI CTAG 7 cut(s) 944, 1344, 1505, 1881, 1907, 2123, 2337
BfmI CTRYAG 3 cut(s) 1245, 1665, 1963
BfoI RGCGCY 1 cut(s) 882
BfrI CTTAAG 1 cut(s) 1094
BfuI GTATCC 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 5 cut(s) 104, 118, 1202, 2283, 2550
Bme18I GGWCC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 2546
BmgT120I GGNCC 2 cut(s) 148, 623
BmiI GGNNCC 2 cut(s) 880, 1930
BmrFI CCNGG 5 cut(s) 104, 118, 1202, 2283, 2550
BmsI GCATC 6 cut(s) 788, 814, 1142, 1507, 1986, 2109
BoxI GACNNNNGTC 2 cut(s) 153, 1560
BpiI GAAGAC 2 cut(s) 157, 1556
BplI GAGNNNNNCTC 2 cut(s) 740, 772
BpmI CTGGAG 4 cut(s) 789, 1169, 1257, 2189
Bpu10I CCTNAGC 1 cut(s) 1850
Bpu14I TTCGAA 1 cut(s) 2464
BpuEI CTTGAG 2 cut(s) 286, 419
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 2 cut(s) 46, 161
BsaJI CCNNGG 7 cut(s) 103, 151, 211, 441, 1201, 1558, 2322
BsaWI WCCGGW 3 cut(s) 429, 1370, 2043
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 536, 645, 2582
Bse1I ACTGG 7 cut(s) 772, 1010, 1115, 1484, 1978, 2184, 2206
Bse3DI GCAATG 2 cut(s) 1509, 1587
BseBI CCWGG 5 cut(s) 104, 118, 1202, 2283, 2550
BseDI CCNNGG 7 cut(s) 103, 151, 211, 441, 1201, 1558, 2322
BseGI GGATG 7 cut(s) 803, 1111, 1228, 1379, 1640, 2230, 2294
BseLI CCNNNNNNNGG 4 cut(s) 76, 536, 645, 2582
BseMI GCAATG 2 cut(s) 1509, 1587
BseMII CTCAG 8 cut(s) 57, 261, 576, 857, 1042, 1484, 1745, 1980
BseNI ACTGG 7 cut(s) 772, 1010, 1115, 1484, 1978, 2184, 2206
BseSI GKGCMC 1 cut(s) 1279
BseXI GCAGC 9 cut(s) 622, 959, 1117, 1231, 1259, 1337, 1654, 1949, 2482
BseYI CCCAGC 1 cut(s) 1923
BsgI GTGCAG 1 cut(s) 1827
BshFI GGCC 5 cut(s) 69, 111, 624, 2136, 2327
BshNI GGYRCC 1 cut(s) 878
BsiHKAI GWGCWC 1 cut(s) 2355
BsiSI CCGG 4 cut(s) 430, 1371, 2044, 2222
BslI CCNNNNNNNGG 4 cut(s) 76, 536, 645, 2582
BsmAI GTCTC 6 cut(s) 46, 161, 221, 302, 2413, 2517
BsnI GGCC 5 cut(s) 69, 111, 624, 2136, 2327
Bso31I GGTCTC 2 cut(s) 46, 161
Bsp119I TTCGAA 1 cut(s) 2464
Bsp1286I GDGCHC 2 cut(s) 1279, 2355
Bsp143I GATC 4 cut(s) 387, 1501, 1750, 2046
Bsp19I CCATGG 1 cut(s) 2322
BspACI CCGC 2 cut(s) 621, 2071
BspANI GGCC 5 cut(s) 69, 111, 624, 2136, 2327
BspCNI CTCAG 8 cut(s) 56, 260, 575, 858, 1041, 1483, 1746, 1981
BspLI GGNNCC 2 cut(s) 880, 1930
BspMAI CTGCAG 2 cut(s) 1249, 1967
BspPI GGATC 1 cut(s) 2054
BspT104I TTCGAA 1 cut(s) 2464
BspT107I GGYRCC 1 cut(s) 878
BspTI CTTAAG 1 cut(s) 1094
BspTNI GGTCTC 2 cut(s) 46, 161
BsrDI GCAATG 2 cut(s) 1509, 1587
BsrI ACTGG 7 cut(s) 772, 1010, 1115, 1484, 1978, 2184, 2206
BssECI CCNNGG 7 cut(s) 103, 151, 211, 441, 1201, 1558, 2322
BssMI GATC 4 cut(s) 387, 1501, 1750, 2046
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 4 cut(s) 151, 211, 441, 2322
Bst2UI CCWGG 5 cut(s) 104, 118, 1202, 2283, 2550
Bst4CI ACNGT 7 cut(s) 124, 659, 719, 899, 1436, 1466, 2298
Bst6I CTCTTC 1 cut(s) 1386
BstACI GRCGYC 1 cut(s) 879
BstAFI CTTAAG 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 2464
BstC8I GCNNGC 3 cut(s) 1214, 1330, 1695
BstDSI CCRYGG 1 cut(s) 2322
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 7 cut(s) 803, 1111, 1228, 1379, 1640, 2230, 2294
BstH2I RGCGCY 1 cut(s) 882
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 4 cut(s) 390, 1504, 1753, 2049
BstMAI GTCTC 6 cut(s) 46, 161, 221, 302, 2413, 2517
BstMBI GATC 4 cut(s) 387, 1501, 1750, 2046
BstMWI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1667, 1943
BstNI CCWGG 5 cut(s) 104, 118, 1202, 2283, 2550
BstNSI RCATGY 4 cut(s) 234, 241, 1697, 2132
BstPAI GACNNNNGTC 2 cut(s) 153, 1560
BstSCI CCNGG 5 cut(s) 102, 116, 1200, 2281, 2548
BstSFI CTRYAG 3 cut(s) 1245, 1665, 1963
BstSLI GKGCMC 1 cut(s) 1279
BstV1I GCAGC 9 cut(s) 622, 959, 1117, 1231, 1259, 1337, 1654, 1949, 2482
BstV2I GAAGAC 2 cut(s) 157, 1556
BstXI CCANNNNNNTGG 2 cut(s) 345, 1208
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 5 cut(s) 69, 111, 624, 2136, 2327
BtgI CCRYGG 1 cut(s) 2322
BtrI CACGTC 1 cut(s) 2546
BtsCI GGATG 7 cut(s) 803, 1111, 1228, 1379, 1640, 2230, 2294
BtsI GCAGTG 2 cut(s) 18, 1803
BtsIMutI CAGTG 8 cut(s) 18, 255, 570, 655, 1036, 1534, 1803, 1971
Cac8I GCNNGC 3 cut(s) 1214, 1330, 1695
CfoI GCGC 1 cut(s) 881
Cfr13I GGNCC 2 cut(s) 148, 623
CseI GACGC 1 cut(s) 1659
Csp6I GTAC 5 cut(s) 895, 978, 1798, 2177, 2242
CviQI GTAC 5 cut(s) 895, 978, 1798, 2177, 2242
DinI GGCGCC 1 cut(s) 880
DpnI GATC 4 cut(s) 389, 1503, 1752, 2048
DpnII GATC 4 cut(s) 387, 1501, 1750, 2046
EaeI YGGCCR 3 cut(s) 109, 2134, 2325
Eam1104I CTCTTC 1 cut(s) 1386
EarI CTCTTC 1 cut(s) 1386
Eco130I CCWWGG 4 cut(s) 151, 211, 441, 2322
Eco147I AGGCCT 1 cut(s) 69
Eco31I GGTCTC 2 cut(s) 46, 161
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 6 cut(s) 506, 516, 581, 1004, 2021, 2577
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 2 cut(s) 2228, 2370
EcoRII CCWGG 5 cut(s) 102, 116, 1200, 2281, 2548
EcoT14I CCWWGG 4 cut(s) 151, 211, 441, 2322
EcoT22I ATGCAT 2 cut(s) 803, 1522
EgeI GGCGCC 1 cut(s) 880
EheI GGCGCC 1 cut(s) 880
ErhI CCWWGG 4 cut(s) 151, 211, 441, 2322
FalI AAGNNNNNCTT 6 cut(s) 54, 86, 311, 343, 2396, 2428
FauNDI CATATG 2 cut(s) 130, 1299
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 2293
FokI GGATG 7 cut(s) 810, 1098, 1235, 1386, 1647, 2237, 2301
FspBI CTAG 7 cut(s) 944, 1344, 1505, 1881, 1907, 2123, 2337
GlaI GCGC 1 cut(s) 880
GsaI CCCAGC 1 cut(s) 1927
GsuI CTGGAG 4 cut(s) 789, 1169, 1257, 2189
HaeII RGCGCY 1 cut(s) 882
HaeIII GGCC 5 cut(s) 69, 111, 624, 2136, 2327
HapII CCGG 4 cut(s) 430, 1371, 2044, 2222
HgaI GACGC 1 cut(s) 1659
HhaI GCGC 1 cut(s) 881
Hin1I GRCGYC 1 cut(s) 879
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 2 cut(s) 1043, 1104
HindII GTYRAC 2 cut(s) 1043, 1104
HindIII AAGCTT 3 cut(s) 304, 547, 1140
HinfI GANTC 5 cut(s) 1601, 2103, 2171, 2340, 2445
HpaII CCGG 4 cut(s) 430, 1371, 2044, 2222
HphI GGTGA 5 cut(s) 358, 685, 1402, 1544, 1796
Hpy166II GTNNAC 7 cut(s) 653, 895, 1043, 1104, 1410, 1462, 2294
Hpy188I TCNGA 7 cut(s) 475, 486, 535, 705, 1023, 1755, 1990
Hpy188III TCNNGA 7 cut(s) 346, 359, 398, 917, 1067, 1907, 2337
Hpy8I GTNNAC 7 cut(s) 653, 895, 1043, 1104, 1410, 1462, 2294
HpyCH4III ACNGT 7 cut(s) 124, 659, 719, 899, 1436, 1466, 2298
HpyCH4IV ACGT 1 cut(s) 2545
HpyF10VI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1667, 1943
HpySE526I ACGT 1 cut(s) 2545
Hsp92I GRCGYC 1 cut(s) 879
HspAI GCGC 1 cut(s) 879
KasI GGCGCC 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 4 cut(s) 387, 1501, 1750, 2046
LmnI GCTCC 3 cut(s) 1150, 1193, 2350
Lsp1109I GCAGC 9 cut(s) 622, 959, 1117, 1231, 1259, 1337, 1654, 1949, 2482
LweI GCATC 6 cut(s) 788, 814, 1142, 1507, 1986, 2109
MaeI CTAG 7 cut(s) 944, 1344, 1505, 1881, 1907, 2123, 2337
MaeII ACGT 1 cut(s) 2545
MaeIII GTNAC 8 cut(s) 124, 413, 459, 917, 1054, 1339, 2311, 2508
MalI GATC 4 cut(s) 389, 1503, 1752, 2048
MboI GATC 4 cut(s) 387, 1501, 1750, 2046
MboII GAAGA 7 cut(s) 157, 334, 679, 1403, 1556, 1835, 2325
MfeI CAATTG 1 cut(s) 2075
MhlI GDGCHC 2 cut(s) 1279, 2355
MlsI TGGCCA 2 cut(s) 111, 2327
MluNI TGGCCA 2 cut(s) 111, 2327
Mly113I GGCGCC 1 cut(s) 879
MlyI GAGTC 2 cut(s) 2180, 2334
MmeI TCCRAC 2 cut(s) 666, 2378
Mox20I TGGCCA 2 cut(s) 111, 2327
Mph1103I ATGCAT 2 cut(s) 803, 1522
MroXI GAANNNNTTC 3 cut(s) 78, 329, 480
MscI TGGCCA 2 cut(s) 111, 2327
MslI CAYNNNNRTG 5 cut(s) 660, 1221, 2094, 2216, 2392
Msp20I TGGCCA 2 cut(s) 111, 2327
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 4 cut(s) 430, 1371, 2044, 2222
MspR9I CCNGG 5 cut(s) 104, 118, 1202, 2283, 2550
MunI CAATTG 1 cut(s) 2075
MvaI CCWGG 5 cut(s) 104, 118, 1202, 2283, 2550
MwoI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1667, 1943
NarI GGCGCC 1 cut(s) 879
NcoI CCATGG 1 cut(s) 2322
NdeI CATATG 2 cut(s) 130, 1299
NdeII GATC 4 cut(s) 387, 1501, 1750, 2046
NlaIV GGNNCC 2 cut(s) 880, 1930
NmuCI GTSAC 4 cut(s) 124, 917, 1054, 2508
NsiI ATGCAT 2 cut(s) 803, 1522
NspI RCATGY 4 cut(s) 234, 241, 1697, 2132
NspV TTCGAA 1 cut(s) 2464
PaeI GCATGC 1 cut(s) 1697
PceI AGGCCT 1 cut(s) 69
PciI ACATGT 2 cut(s) 237, 2128
PdmI GAANNNNTTC 3 cut(s) 78, 329, 480
PfeI GAWTC 3 cut(s) 1601, 2103, 2445
PflMI CCANNNNNTGG 1 cut(s) 645
PleI GAGTC 2 cut(s) 2179, 2334
PluTI GGCGCC 1 cut(s) 882
PpsI GAGTC 2 cut(s) 2179, 2334
PscI ACATGT 2 cut(s) 237, 2128
PshAI GACNNNNGTC 2 cut(s) 153, 1560
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 5 cut(s) 102, 116, 1200, 2281, 2548
PspFI CCCAGC 1 cut(s) 1923
PspGI CCWGG 5 cut(s) 102, 116, 1200, 2281, 2548
PspN4I GGNNCC 2 cut(s) 880, 1930
PspPI GGNCC 2 cut(s) 148, 623
PsrI GAACNNNNNNTAC 2 cut(s) 855, 887
PstI CTGCAG 2 cut(s) 1249, 1967
RsaI GTAC 5 cut(s) 896, 979, 1799, 2178, 2243
RsaNI GTAC 5 cut(s) 895, 978, 1798, 2177, 2242
RseI CAYNNNNRTG 5 cut(s) 660, 1221, 2094, 2216, 2392
Sau3AI GATC 4 cut(s) 387, 1501, 1750, 2046
Sau96I GGNCC 2 cut(s) 148, 623
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 2 cut(s) 2180, 2334
ScrFI CCNGG 5 cut(s) 104, 118, 1202, 2283, 2550
SduI GDGCHC 2 cut(s) 1279, 2355
SfaNI GCATC 6 cut(s) 788, 814, 1142, 1507, 1986, 2109
SfcI CTRYAG 3 cut(s) 1245, 1665, 1963
SfoI GGCGCC 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 2464
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 5 cut(s) 660, 1221, 2094, 2216, 2392
SmlI CTYRAG 3 cut(s) 301, 398, 1094
SmoI CTYRAG 3 cut(s) 301, 398, 1094
SphI GCATGC 1 cut(s) 1697
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 2 cut(s) 621, 2071
SspDI GGCGCC 1 cut(s) 878
SspI AATATT 3 cut(s) 837, 1812, 2477
SspMI CTAG 7 cut(s) 944, 1344, 1505, 1881, 1907, 2123, 2337
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 5 cut(s) 102, 116, 1200, 2281, 2548
StyI CCWWGG 4 cut(s) 151, 211, 441, 2322
TaaI ACNGT 7 cut(s) 124, 659, 719, 899, 1436, 1466, 2298
TaiI ACGT 1 cut(s) 2548
TaqI TCGA 3 cut(s) 1136, 1726, 2464
TaqII GACCGA 1 cut(s) 1572
TatI WGTACW 4 cut(s) 894, 977, 1797, 2176
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 3 cut(s) 1601, 2103, 2445
TscAI CASTG 8 cut(s) 18, 255, 570, 662, 1036, 1534, 1810, 1978
TseFI GTSAC 4 cut(s) 124, 917, 1054, 2508
TseI GCWGC 9 cut(s) 610, 947, 1130, 1244, 1247, 1325, 1642, 1962, 2495
Tsp45I GTSAC 4 cut(s) 124, 917, 1054, 2508
TspGWI ACGGA 1 cut(s) 1331
TspRI CASTG 8 cut(s) 18, 255, 570, 662, 1036, 1534, 1810, 1978
Van91I CCANNNNNTGG 1 cut(s) 645
Vha464I CTTAAG 1 cut(s) 1094
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 8 cut(s) 1179, 1857, 2003, 2141, 2228, 2370, 2407, 2455
XbaI TCTAGA 2 cut(s) 1906, 2336
XceI RCATGY 4 cut(s) 234, 241, 1697, 2132
XmiI GTMKAC 1 cut(s) 2293
XmnI GAANNNNTTC 3 cut(s) 78, 329, 480
XspI CTAG 7 cut(s) 944, 1344, 1505, 1881, 1907, 2123, 2337
ZrmI AGTACT 1 cut(s) 979
Zsp2I ATGCAT 2 cut(s) 803, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.