RLG00000013591

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000555
Physical Location & Seq
Forward (+)
33723930 .. 33726414
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013591

Sequence Viewer

Length: 1161 bp
ATGGCTCTTTCGGACAACCCAAAACTTATGGCTCTTTCATTGACGGAAACAGCGGCACGCATATTTGTTACTAGATTTCTTAGACATTATATATCTTCTGGATGTTTAATCTTATTAGAAGAGGGAGGCACAGTGTTCACCTTTGAGGGAAGCAAAAAGGATTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCACTTCTACTGGAAAGTAATGACACAGGCTGATCTAGGCATTGCAGATGCATATATTAATGGAGACTTTTCTTTTGATGATAAAGACCGAGGTCTTCTAAATCTTTTTATGCTTCTCATTGCCAACGGAGATTCTAATGCCTCAAAATCAAACAAGAAAAGGAGATGGTGGCAGCCTTTGTTTTTCACAGCGACTATAGCCTCAGCCAAATATGTCTTTCAGCATGCTTTAAGGAAAAATAGTCTTACACAAGCTCGAAGGAACATATCTCGTCATTATGATCTGAGTAATGAACTATTTTCGCTGTTCTTGGGTGAAACAATGATGTATTCCACTGCAATATTTAAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATTTCTACTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGATTCTAGAAATTGGTTGTGGTTGGGGAGCCTTGGCTATTGAAGCAGTCAAACAAACGGGTTGCAGATACACTGGCATCACTCTATCTGAAGAGCAACTGAAATTTGCTGAAAAAAAAGTGAAAGATGCTGGACTTCAGGACCGGATCAAGTTTCTTCTGTGTGACTACCGCCAATTGCCTTCTGACCACAAATATGACAGAATCATAATTTGCGAGGTGCTAGAACATATCGGCCATGAATTTGTGGATGAGTTTTTTTCTTGCTGTGAGTCTTTACTAACAGAAAATGGGATTCTTGTTCTGCAGTTCATATCTATACTGGATGAACTCTATGATGAGTACAGGCGAAGTCCAGGCTTCATAAAGGAATATATATTTCCTGGTGGACATCTACTTTCATTAAGTAGGGTAACATCAGCCATGGCCGCTTCTTCTAGACTCTGTGTGGAGCACTTGGAAAACATTGGACACCATCATTATTACCAAACACTCAGATGTTGGAGAAAGAATTTTTTAGAGAGACAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

387

Amino Acids

44.45

Weight (kDa)

6.92

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 148 - 384 1.2e-66 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 194 - 314 8.3e-10 Methyltransferase domain
Methyltransf_31 PF13847 206 - 328 3.8e-14 Methyltransferase domain
MTS PF05175 206 - 311 1.3e-06 Methyltransferase small domain
Methyltransf_25 PF13649 211 - 307 9.5e-17 Methyltransferase domain
Methyltransf_11 PF08241 212 - 311 2.4e-14 Methyltransferase domain
Methyltransf_12 PF08242 212 - 309 4.7e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 53, 799, 1056
AclWI GGATC 1 cut(s) 782
AcoI YGGCCR 2 cut(s) 862, 1053
AcsI RAATTY 4 cut(s) 585, 731, 869, 1138
AcuI CTGAAG 2 cut(s) 738, 749
AfaI GTAC 1 cut(s) 971
AgsI TTSAA 1 cut(s) 671
AjnI CCWGG 2 cut(s) 982, 1009
AluBI AGCT 2 cut(s) 452, 577
AluI AGCT 2 cut(s) 452, 577
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 2 cut(s) 255, 1144
AlwI GGATC 1 cut(s) 782
AoxI GGCC 2 cut(s) 862, 1053
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 4 cut(s) 585, 731, 869, 1138
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AseI ATTAAT 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 769
AsuHPI GGTGA 2 cut(s) 130, 524
AvaII GGWCC 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 1083
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 382
BccI CCATC 2 cut(s) 357, 1110
BciT130I CCWGG 2 cut(s) 984, 1011
BcoDI GTCTC 2 cut(s) 255, 1144
BfaI CTAG 6 cut(s) 72, 233, 609, 635, 851, 1065
BfmI CTRYAG 2 cut(s) 393, 932
BisI GCNGC 3 cut(s) 54, 371, 1056
BlsI GCNGC 3 cut(s) 55, 372, 1057
Bme1390I CCNGG 2 cut(s) 984, 1011
Bme18I GGWCC 1 cut(s) 769
BmgT120I GGNCC 1 cut(s) 769
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 984, 1011
BmsI GCATC 3 cut(s) 235, 714, 745
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 2 cut(s) 400, 578
Bsa29I ATCGAT 1 cut(s) 615
BsaJI CCNNGG 3 cut(s) 286, 660, 1050
BsaWI WCCGGW 1 cut(s) 771
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bse1I ACTGG 3 cut(s) 212, 706, 954
Bse3DI GCAATG 2 cut(s) 237, 315
BseBI CCWGG 2 cut(s) 984, 1011
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 286, 660, 1050
BseGI GGATG 3 cut(s) 107, 883, 958
BseMI GCAATG 2 cut(s) 237, 315
BseMII CTCAG 3 cut(s) 414, 473, 1135
BseNI ACTGG 3 cut(s) 212, 706, 954
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 2 cut(s) 864, 1055
BshVI ATCGAT 1 cut(s) 615
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 255, 1144
BsnI GGCC 2 cut(s) 864, 1055
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp143I GATC 3 cut(s) 229, 478, 774
Bsp19I CCATGG 1 cut(s) 1050
BspACI CCGC 3 cut(s) 53, 799, 1056
BspANI GGCC 2 cut(s) 864, 1055
BspCNI CTCAG 3 cut(s) 413, 474, 1134
BspDI ATCGAT 1 cut(s) 615
BspLI GGNNCC 1 cut(s) 658
BspMAI CTGCAG 1 cut(s) 936
BspPI GGATC 1 cut(s) 782
BspQI GCTCTTC 1 cut(s) 714
BsrDI GCAATG 2 cut(s) 237, 315
BsrI ACTGG 3 cut(s) 212, 706, 954
BssECI CCNNGG 3 cut(s) 286, 660, 1050
BssMI GATC 3 cut(s) 229, 478, 774
BssT1I CCWWGG 2 cut(s) 660, 1050
Bst2UI CCWGG 2 cut(s) 984, 1011
Bst4CI ACNGT 2 cut(s) 133, 194
Bst6I CTCTTC 2 cut(s) 114, 714
BstC8I GCNNGC 2 cut(s) 58, 423
BstDEI CTNAG 5 cut(s) 80, 400, 482, 578, 1121
BstDSI CCRYGG 1 cut(s) 1050
BstF5I GGATG 3 cut(s) 107, 883, 958
BstKTI GATC 3 cut(s) 232, 481, 777
BstMAI GTCTC 2 cut(s) 255, 1144
BstMBI GATC 3 cut(s) 229, 478, 774
BstMWI GCNNNNNNNGC 3 cut(s) 395, 671, 1055
BstNI CCWGG 2 cut(s) 984, 1011
BstNSI RCATGY 1 cut(s) 425
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 982, 1009
BstSFI CTRYAG 2 cut(s) 393, 932
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 284
Bsu15I ATCGAT 1 cut(s) 615
BsuRI GGCC 2 cut(s) 864, 1055
BsuTUI ATCGAT 1 cut(s) 615
BtgI CCRYGG 1 cut(s) 1050
BtsCI GGATG 3 cut(s) 107, 883, 958
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 3 cut(s) 138, 531, 699
Cac8I GCNNGC 2 cut(s) 58, 423
Cfr13I GGNCC 1 cut(s) 769
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 1 cut(s) 970
CviAII CATG 4 cut(s) 422, 626, 866, 1051
CviQI GTAC 1 cut(s) 970
DdeI CTNAG 5 cut(s) 80, 400, 482, 578, 1121
DpnI GATC 3 cut(s) 231, 480, 776
DpnII GATC 3 cut(s) 229, 478, 774
EaeI YGGCCR 2 cut(s) 862, 1053
Eam1104I CTCTTC 2 cut(s) 114, 714
EarI CTCTTC 2 cut(s) 114, 714
Eco130I CCWWGG 2 cut(s) 660, 1050
Eco47I GGWCC 1 cut(s) 769
Eco57I CTGAAG 2 cut(s) 738, 749
EcoRII CCWGG 2 cut(s) 982, 1009
EcoT14I CCWWGG 2 cut(s) 660, 1050
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 2 cut(s) 660, 1050
FaeI CATG 4 cut(s) 425, 629, 869, 1054
FatI CATG 4 cut(s) 421, 625, 865, 1050
Fnu4HI GCNGC 3 cut(s) 54, 371, 1056
FokI GGATG 3 cut(s) 114, 890, 965
Fsp4HI GCNGC 3 cut(s) 54, 371, 1056
FspBI CTAG 6 cut(s) 72, 233, 609, 635, 851, 1065
GluI GCNGC 3 cut(s) 54, 371, 1056
HaeIII GGCC 2 cut(s) 864, 1055
HapII CCGG 1 cut(s) 772
Hin1II CATG 4 cut(s) 425, 629, 869, 1054
HinfI GANTC 7 cut(s) 329, 612, 631, 831, 899, 922, 1068
HpaII CCGG 1 cut(s) 772
HphI GGTGA 2 cut(s) 130, 524
Hpy166II GTNNAC 3 cut(s) 138, 190, 1016
Hpy188I TCNGA 5 cut(s) 13, 483, 718, 814, 1124
Hpy188III TCNNGA 4 cut(s) 99, 635, 767, 1065
Hpy8I GTNNAC 3 cut(s) 138, 190, 1016
HpyAV CCTTC 2 cut(s) 450, 819
HpyCH4III ACNGT 2 cut(s) 133, 194
HpyCH4V TGCA 6 cut(s) 242, 248, 536, 572, 693, 934
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 671, 1055
HpyF3I CTNAG 5 cut(s) 80, 400, 482, 578, 1121
Hsp92II CATG 4 cut(s) 425, 629, 869, 1054
Kzo9I GATC 3 cut(s) 229, 478, 774
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 2 cut(s) 656, 1078
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 3 cut(s) 235, 714, 745
MaeI CTAG 6 cut(s) 72, 233, 609, 635, 851, 1065
MaeIII GTNAC 3 cut(s) 67, 791, 1039
MalI GATC 3 cut(s) 231, 480, 776
MboI GATC 3 cut(s) 229, 478, 774
MboII GAAGA 7 cut(s) 87, 131, 284, 563, 731, 776, 1053
MfeI CAATTG 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 1083
MluCI AATT 7 cut(s) 585, 639, 731, 803, 837, 869, 1138
MlyI GAGTC 2 cut(s) 908, 1062
MmeI TCCRAC 1 cut(s) 1109
Mph1103I ATGCAT 1 cut(s) 250
MseI TTAA 5 cut(s) 107, 255, 428, 543, 1031
MslI CAYNNNNRTG 2 cut(s) 822, 1123
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 772
MspR9I CCNGG 2 cut(s) 984, 1011
MunI CAATTG 1 cut(s) 803
MvaI CCWGG 2 cut(s) 984, 1011
MwoI GCNNNNNNNGC 3 cut(s) 395, 671, 1055
NcoI CCATGG 1 cut(s) 1050
NdeII GATC 3 cut(s) 229, 478, 774
NlaIII CATG 4 cut(s) 425, 629, 869, 1054
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 1 cut(s) 425
PaeI GCATGC 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 714
PfeI GAWTC 5 cut(s) 329, 612, 631, 831, 922
PkrI GCNGC 3 cut(s) 55, 372, 1057
PleI GAGTC 2 cut(s) 907, 1062
PpsI GAGTC 2 cut(s) 907, 1062
PshAI GACNNNNGTC 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 982, 1009
PspGI CCWGG 2 cut(s) 982, 1009
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 1 cut(s) 769
PstI CTGCAG 1 cut(s) 936
RsaI GTAC 1 cut(s) 971
RsaNI GTAC 1 cut(s) 970
RseI CAYNNNNRTG 2 cut(s) 822, 1123
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 5 cut(s) 107, 255, 428, 543, 1031
SatI GCNGC 3 cut(s) 54, 371, 1056
Sau3AI GATC 3 cut(s) 229, 478, 774
Sau96I GGNCC 1 cut(s) 769
SchI GAGTC 2 cut(s) 908, 1062
ScrFI CCNGG 2 cut(s) 984, 1011
SduI GDGCHC 1 cut(s) 1083
SetI ASST 6 cut(s) 143, 292, 454, 579, 849, 1160
SfaNI GCATC 3 cut(s) 235, 714, 745
SfcI CTRYAG 2 cut(s) 393, 932
SinI GGWCC 1 cut(s) 769
SmiMI CAYNNNNRTG 2 cut(s) 822, 1123
SphI GCATGC 1 cut(s) 425
Sse9I AATT 7 cut(s) 585, 639, 731, 803, 837, 869, 1138
SsiI CCGC 3 cut(s) 53, 799, 1056
SspI AATATT 1 cut(s) 540
SspMI CTAG 6 cut(s) 72, 233, 609, 635, 851, 1065
StyD4I CCNGG 2 cut(s) 982, 1009
StyI CCWWGG 2 cut(s) 660, 1050
TaaI ACNGT 2 cut(s) 133, 194
TaqI TCGA 2 cut(s) 454, 615
TaqII GACCGA 1 cut(s) 300
TasI AATT 7 cut(s) 585, 639, 731, 803, 837, 869, 1138
TatI WGTACW 1 cut(s) 969
TauI GCSGC 2 cut(s) 56, 1058
TfiI GAWTC 5 cut(s) 329, 612, 631, 831, 922
Tru1I TTAA 5 cut(s) 107, 255, 428, 543, 1031
Tru9I TTAA 5 cut(s) 107, 255, 428, 543, 1031
TscAI CASTG 3 cut(s) 138, 538, 706
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 7 cut(s) 27, 504, 882, 928, 969, 979, 1017
TspGWI ACGGA 3 cut(s) 59, 166, 339
TspRI CASTG 3 cut(s) 138, 538, 706
VpaK11BI GGWCC 1 cut(s) 769
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 4 cut(s) 585, 731, 869, 1138
XbaI TCTAGA 2 cut(s) 634, 1064
XceI RCATGY 1 cut(s) 425
XspI CTAG 6 cut(s) 72, 233, 609, 635, 851, 1065
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.