Rh1AG305900

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000555
Physical Location & Seq
Forward (+)
53883409 .. 53895355
11947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG305900.1

Sequence Viewer

Length: 765 bp
ATGGCTGCAGCAACTGGTGTGCTTGGAAAAGGTTGTGCGCTCTTGGAGAAGGTCGCACGCCTATATGTTACTAGATTTCTGAGACAGTATATCTCTACAGGATGTTTAGTCTTATTAGAAGAAAGAATGGTGCAAACCTTTGAGGGAAGCAAGAAAGGCTGTTCTTTGAAATCTGTTATTAAAGTTCATAACCCCGTTCAATTTTACTGGAAGGCTAGAATTACTAAGGACCATGAAGTCCTAGAGTTGGGCTGTGGTTGGGGAGGCTTCGCCATTGAAGTCGTCAAGCAAACCGGTTGTAGATACACTGGCATAACTTTGTCGAAGGAACGAGTGCAATTTGCTGAAAAGAAAGTGAATGATGCTGGCCTTCAGGAAAGCATCAAAATTATGCTGCGTGACTACCGCGAATTGCCTCAGAACTACAAATATGACAGAATTATTTGTTGTGAGATGCTAGAACATGTCGGCCATGAATTTATGGAGGAATTCTTTTCTTGTTGTGACTCTATGCTAGCAGAAAATGGGCTTCTTATTCTACAGTTCACATTCTTTCCGGATGAGCGTTACGATGAATATAGGCGAAGTTCAGACTTCATAAAGGAGTATATTTTTCCGGGTGGATTGTTACCTTCATTAAGTAGGGTAACATCAGCTATGGTTGCTTCTTCCAGGTTCAGTGTGCAGCACTTGGAAAACATTGGAATTCATTATTACCAAACACTAAGATATTGGAGAAAAACATTCTCGGAGAAACATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

254

Amino Acids

29.47

Weight (kDa)

8.65

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 71 - 252 3e-46 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 74 - 182 7.8e-11 Methyltransferase domain
Methyltransf_31 PF13847 75 - 176 3.2e-09 Methyltransferase domain
MTS PF05175 76 - 180 7.2e-07 Methyltransferase small domain
Methyltransf_25 PF13649 80 - 176 2.9e-15 Methyltransferase domain
Methyltransf_11 PF08241 81 - 180 1.8e-12 Methyltransferase domain
Methyltransf_12 PF08242 81 - 178 3.8e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 3 cut(s) 476, 488, 705
AcuI CTGAAG 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 463
AgeI ACCGGT 1 cut(s) 293
AgsI TTSAA 3 cut(s) 169, 200, 278
AjnI CCWGG 1 cut(s) 671
AluBI AGCT 1 cut(s) 656
AluI AGCT 1 cut(s) 656
Alw26I GTCTC 1 cut(s) 76
AlwNI CAGNNNCTG 1 cut(s) 14
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 2 cut(s) 367, 469
ApeKI GCWGC 4 cut(s) 5, 8, 394, 685
ApoI RAATTY 3 cut(s) 476, 488, 705
AsiGI ACCGGT 1 cut(s) 293
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 618
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 1 cut(s) 229
BbvI GCAGC 3 cut(s) 20, 381, 697
BciT130I CCWGG 1 cut(s) 673
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 5 cut(s) 72, 216, 242, 458, 515
BfmI CTRYAG 3 cut(s) 6, 96, 539
BisI GCNGC 4 cut(s) 6, 9, 395, 686
BlsI GCNGC 4 cut(s) 7, 10, 396, 687
Bme1390I CCNGG 2 cut(s) 618, 673
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 229
BmrFI CCNGG 2 cut(s) 618, 673
BmsI GCATC 3 cut(s) 352, 390, 444
BmtI GCTAGC 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 618
BsaWI WCCGGW 2 cut(s) 293, 556
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 3 cut(s) 19, 212, 313
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 1 cut(s) 673
BseGI GGATG 2 cut(s) 107, 565
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 2 cut(s) 71, 431
BseNI ACTGG 3 cut(s) 19, 212, 313
BseXI GCAGC 3 cut(s) 20, 381, 697
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 369, 471
BshTI ACCGGT 1 cut(s) 293
BsiSI CCGG 3 cut(s) 294, 557, 617
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 76
BsnI GGCC 2 cut(s) 369, 471
Bsp13I TCCGGA 1 cut(s) 556
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 369, 471
BspCNI CTCAG 2 cut(s) 72, 430
BspEI TCCGGA 1 cut(s) 556
BspFNI CGCG 1 cut(s) 408
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 518
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 3 cut(s) 19, 212, 313
BssAI RCCGGY 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 673
Bst4CI ACNGT 2 cut(s) 87, 543
BstC8I GCNNGC 3 cut(s) 58, 367, 516
BstDEI CTNAG 4 cut(s) 80, 225, 417, 725
BstF5I GGATG 2 cut(s) 107, 565
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 1 cut(s) 40
BstMAI GTCTC 1 cut(s) 76
BstMWI GCNNNNNNNGC 2 cut(s) 156, 662
BstNI CCWGG 1 cut(s) 673
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 616, 671
BstSFI CTRYAG 3 cut(s) 6, 96, 539
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 20, 381, 697
BsuRI GGCC 2 cut(s) 369, 471
BtsCI GGATG 2 cut(s) 107, 565
BtsIMutI CAGTG 2 cut(s) 306, 685
Cac8I GCNNGC 3 cut(s) 58, 367, 516
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 229
CspAI ACCGGT 1 cut(s) 293
CviAII CATG 3 cut(s) 233, 464, 473
CviJI RGCY 9 cut(s) 5, 159, 215, 252, 267, 369, 471, 529, 656
CviKI_1 RGCY 9 cut(s) 5, 159, 215, 252, 267, 369, 471, 529, 656
DdeI CTNAG 4 cut(s) 80, 225, 417, 725
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 356
EcoRI GAATTC 2 cut(s) 488, 705
EcoRII CCWGG 1 cut(s) 671
FaeI CATG 3 cut(s) 236, 467, 476
FatI CATG 3 cut(s) 232, 463, 472
Fnu4HI GCNGC 4 cut(s) 6, 9, 395, 686
FokI GGATG 2 cut(s) 114, 572
Fsp4HI GCNGC 4 cut(s) 6, 9, 395, 686
FspBI CTAG 5 cut(s) 72, 216, 242, 458, 515
GlaI GCGC 1 cut(s) 39
GluI GCNGC 4 cut(s) 6, 9, 395, 686
HaeIII GGCC 2 cut(s) 369, 471
HapII CCGG 3 cut(s) 294, 557, 617
HhaI GCGC 1 cut(s) 40
Hin1II CATG 3 cut(s) 236, 467, 476
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 1 cut(s) 506
HpaII CCGG 3 cut(s) 294, 557, 617
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 4 cut(s) 81, 420, 592, 751
Hpy188III TCNNGA 2 cut(s) 374, 557
Hpy8I GTNNAC 1 cut(s) 546
HpyAV CCTTC 5 cut(s) 43, 205, 319, 380, 642
HpyCH4III ACNGT 2 cut(s) 87, 543
HpyCH4V TGCA 4 cut(s) 8, 133, 337, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 662
HpyF3I CTNAG 4 cut(s) 80, 225, 417, 725
Hsp92II CATG 3 cut(s) 236, 467, 476
HspAI GCGC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 556
Lsp1109I GCAGC 3 cut(s) 20, 381, 697
LweI GCATC 3 cut(s) 352, 390, 444
MaeI CTAG 5 cut(s) 72, 216, 242, 458, 515
MaeIII GTNAC 6 cut(s) 67, 398, 503, 566, 627, 646
MboII GAAGA 2 cut(s) 131, 660
MluCI AATT 9 cut(s) 200, 219, 338, 387, 410, 438, 476, 488, 705
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 4 cut(s) 136, 257, 426, 478
MroI TCCGGA 1 cut(s) 556
MseI TTAA 2 cut(s) 180, 638
MspI CCGG 3 cut(s) 294, 557, 617
MspR9I CCNGG 2 cut(s) 618, 673
MvaI CCWGG 1 cut(s) 673
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 156, 662
NciI CCSGG 1 cut(s) 618
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 3 cut(s) 236, 467, 476
NmuCI GTSAC 2 cut(s) 398, 503
NspI RCATGY 1 cut(s) 467
PciI ACATGT 1 cut(s) 463
PinAI ACCGGT 1 cut(s) 293
PkrI GCNGC 4 cut(s) 7, 10, 396, 687
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
PscI ACATGT 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 671
PspGI CCWGG 1 cut(s) 671
PspPI GGNCC 1 cut(s) 229
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 14
SaqAI TTAA 2 cut(s) 180, 638
SatI GCNGC 4 cut(s) 6, 9, 395, 686
Sau96I GGNCC 1 cut(s) 229
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 618, 673
SetI ASST 7 cut(s) 34, 54, 140, 634, 658, 677, 764
SfaNI GCATC 3 cut(s) 352, 390, 444
SfcI CTRYAG 3 cut(s) 6, 96, 539
SinI GGWCC 1 cut(s) 229
Sse9I AATT 9 cut(s) 200, 219, 338, 387, 410, 438, 476, 488, 705
SsiI CCGC 1 cut(s) 406
SspMI CTAG 5 cut(s) 72, 216, 242, 458, 515
StyD4I CCNGG 2 cut(s) 616, 671
TaaI ACNGT 2 cut(s) 87, 543
TaqI TCGA 1 cut(s) 323
TasI AATT 9 cut(s) 200, 219, 338, 387, 410, 438, 476, 488, 705
Tru1I TTAA 2 cut(s) 180, 638
Tru9I TTAA 2 cut(s) 180, 638
TscAI CASTG 2 cut(s) 313, 685
TseFI GTSAC 2 cut(s) 398, 503
TseI GCWGC 4 cut(s) 5, 8, 394, 685
Tsp45I GTSAC 2 cut(s) 398, 503
TspDTI ATGAA 7 cut(s) 176, 249, 489, 586, 588, 624, 698
TspRI CASTG 2 cut(s) 313, 685
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 3 cut(s) 476, 488, 705
XceI RCATGY 1 cut(s) 467
XspI CTAG 5 cut(s) 72, 216, 242, 458, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.