FvH4_7g18110

Mycolic acid cyclopropane synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000555
Physical Location & Seq
Reverse (-)
15212580 .. 15216869
4290 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18110.t1

Sequence Viewer

Length: 1320 bp
ATGGTGGAGTTGGAATCAATGGCTTCAGCAATATTGGAAAGGGTCTCTCGCCTATATGTTACCAAATTTCTACGACAGTATGTCTCTACAGGATGTTTGATCTTATCAGAAGATGGAGGAACGGTAGAAACCTTTGAGGGAACCAAAAAAGGCTGTTCTTTGAAATGTGTTCTTCAAGTTCATGATCCCGCTCAATTTTACTGGAAGGTAATGACAAGGGCAGATCTAGGCCTTGCAGATGCATATATCAATGGTGATTTTTCTTTAGCTGATAAAGACAAGGGTCTGGAGAATCTCTTTACGATTCTTAATGCCAACAGAGATTCAACTTCTCCACTCTCAAAATTGAACAAGATAAGAAGAAGATTACTGGAAGCTTGTTTATCCCCAGCAAGATATCTCTTTCACCACGGTTCAGGGCAAAACACACTTACAGTGGCTCGAAGGAACGTCTCTCGCCATTATGACTTGAGTAATGCACTATTTTCGCTCTTCTTGGACGAAACAATGTCGTATTCCACTGCTGTATTCAAGAAAGATGATGAAGATTTGAAGACTGGACAGCTAAGGAAAATCAAGACTCTCATTGACAAGGCTAGAATTACTAAGAACCATGAAGTCCTAGAGGTAGGCTGTGGTTGGGGAGGCTTAGCTATCGAAGTCGTCAAGCAAACCGGTTGTAGATATACTGGCATAACTTTGTCGAAGGAACAACTGCAATTTGCCGAACAGAGAGTGAAAGATGCTGGACTTGAGGAAAACATCAAATTTCTGCTGTGTGACTACCGCACATTGCCTAAGAACTACAAATATGACAGAATTGTTAGTTGCGAGATGGTAGAACATGTCGGCCATGAATATATGGAGGAATTCTTTGCTTGCTGTGACTCTGCTCTAGCAGAAAATGGGCTTCTCGTTCTACAATTCACATCATTACCGGATGAGCTTTACGACGAGTACAGGCGATGTCCAAGCTTCATAAGGGAGTATATTCTTCCAGGTGGATGTTTACCTTCGTTAAGTAGGTTAACAGCTGCCATGGCTGCTTCTTCCAGGCTTAGTGTGGAGCACTTGGAAAACATTGGAAGTCATTATTACCGAACACTAAAATATTGGAGGAAAAATTTCTTGGAGAAACAAAGTGAAATACAAGCTCTTGGATTCGATGACAAGTTTATTCGGACATGGGAATATTATTTTGATTATTGTGCGGCAGGATTCAACGCTTGTACACTTGGAAATTACCAGATTGTGTTTTCACGTCCCGGTAATGTTGTTCTGCTCAACGATCCATACAAAGGCTTCCCTTCAGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase CMAS NAD_binding_8

Protein Analysis

440

Amino Acids

50.05

Weight (kDa)

6.76

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 146 - 412 4.6e-77 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 192 - 315 1.4e-09 Methyltransferase domain
MTS PF05175 197 - 307 2.4e-06 Methyltransferase small domain
Methyltransf_31 PF13847 202 - 311 6.7e-12 Methyltransferase domain
Methyltransf_25 PF13649 207 - 303 8.2e-17 Methyltransferase domain
Methyltransf_11 PF08241 208 - 307 1.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 208 - 305 7.7e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23470 AT3G23470 AT3G23470 AT3G23480 AT3G23480 AT3G23480 AT3G23510 AT3G23530
fragaria_vesca FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04310 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_4g04360 FvH4_7g18110 FvH4_7g18140 FvH4_7g18480 FvH4_7g18481
malus_domestica MD16G1224400.v1.1
prunus_persica Prupe.1G045800_v2.0.a1
pyrus_communis pycom16g18780
rosa_chinensis RchiOBHm_Chr1g0325031 RchiOBHm_Chr1g0362281 RchiOBHm_Chr4g0394761 RchiOBHm_Chr4g0394801 RchiOBHm_Chr4g0394871 RchiOBHm_Chr4g0394921 RchiOBHm_Chr4g0394931 RchiOBHm_Chr4g0394941 RchiOBHm_Chr6g0273911
rosa_laevigata RLG00000009657 RLG00000009659 RLG00000009665 RLG00000013591 RLG00000027657
rosa_multiflora Rmu_sc0005356.1_g000006 Rmu_sc0005802.1_g000020 Rmu_sc0007125.1_g000012 Rmu_ssc0000009.1_g000024
rosa_roxburghii Rroxscaffold_5G00339800 Rroxscaffold_5G00339890 Rroxscaffold_7G00194930
rosa_rugosa Rorug01G0197800 Rorug01G0297000 Rorug01G0297100 Rorug03G0360900.1 Rorug03G0361000.1 Rorug03G0361200.1 Rorug03G0361700.1 Rorug03G0361800.1 Rorug03G0361900.1 Rorug04G0078200
rosa_samantha Rh1AG090300 Rh1AG305900 Rh1DG071500 Rh1DG299200 Rh1DG299300 Rh1DG299400 Rh4AG054900 Rh4BG052700 Rh4CG059500 Rh4CG060100 Rh4CG060200 Rh4DG051300 Rh4DG051800 Rh5BG441600 Rh6AG193200 Rh6CG196400
rosa_wichuraiana Rw1G027140 Rw4G003970 Rw4G004380 Rw4G004420 Rw4G004440 Rw6G016840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 191
AciI CCGC 3 cut(s) 189, 787, 1211
AclWI GGATC 2 cut(s) 179, 1283
AcoI YGGCCR 1 cut(s) 850
AcsI RAATTY 4 cut(s) 65, 767, 869, 1123
AcuI CTGAAG 2 cut(s) 9, 1293
AfaI GTAC 2 cut(s) 959, 1231
AfiI CCNNNNNNNGG 1 cut(s) 1298
AflIII ACRYGT 1 cut(s) 844
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 7 cut(s) 163, 176, 327, 349, 532, 553, 1222
AhdI GACNNNNNGTC 1 cut(s) 80
AjiI CACGTC 1 cut(s) 1262
AjnI CCWGG 2 cut(s) 997, 1052
AjuI GAANNNNNNNTTGG 2 cut(s) 1112, 1144
AluBI AGCT 8 cut(s) 269, 377, 565, 653, 946, 975, 1034, 1154
AluI AGCT 8 cut(s) 269, 377, 565, 653, 946, 975, 1034, 1154
Alw21I GWGCWC 1 cut(s) 1071
Alw26I GTCTC 3 cut(s) 49, 88, 457
AlwI GGATC 2 cut(s) 179, 1283
AoxI GGCC 2 cut(s) 229, 850
ApeKI GCWGC 2 cut(s) 1034, 1043
ApoI RAATTY 4 cut(s) 65, 767, 869, 1123
AsiGI ACCGGT 1 cut(s) 674
Asp700I GAANNNNTTC 1 cut(s) 1124
AsuC2I CCSGG 1 cut(s) 1266
AsuHPI GGTGA 2 cut(s) 266, 398
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 1 cut(s) 1071
BbvI GCAGC 2 cut(s) 1021, 1030
BccI CCATC 2 cut(s) 107, 829
BcgI CGANNNNNNTGC 2 cut(s) 468, 502
BciT130I CCWGG 2 cut(s) 999, 1054
BcnI CCSGG 1 cut(s) 1266
BcoDI GTCTC 3 cut(s) 49, 88, 457
BfaI CTAG 4 cut(s) 227, 597, 623, 896
BfmI CTRYAG 1 cut(s) 87
BglII AGATCT 1 cut(s) 223
BisI GCNGC 3 cut(s) 1035, 1044, 1212
BlpI GCTNAGC 1 cut(s) 649
BlsI GCNGC 3 cut(s) 1036, 1045, 1213
Bme1390I CCNGG 3 cut(s) 999, 1054, 1266
BmeRI GACNNNNNGTC 1 cut(s) 80
BmgBI CACGTC 1 cut(s) 1262
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 3 cut(s) 999, 1054, 1266
BmsI GCATC 2 cut(s) 229, 733
BoxI GACNNNNGTC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 560
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 566
Bpu1102I GCTNAGC 1 cut(s) 649
BpuEI CTTGAG 2 cut(s) 490, 773
BpuMI CCSGG 1 cut(s) 1266
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 409, 1038
BsaWI WCCGGW 2 cut(s) 674, 937
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 1298
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 4 cut(s) 206, 375, 562, 694
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 2 cut(s) 999, 1054
BseDI CCNNGG 2 cut(s) 409, 1038
BseGI GGATG 3 cut(s) 98, 946, 1010
BseLI CCNNNNNNNGG 1 cut(s) 1298
BseMI GCAATG 1 cut(s) 791
BseNI ACTGG 4 cut(s) 206, 375, 562, 694
BseXI GCAGC 2 cut(s) 1021, 1030
BseYI CCCAGC 1 cut(s) 388
BshFI GGCC 2 cut(s) 231, 852
BshTI ACCGGT 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 1071
BsiSI CCGG 3 cut(s) 675, 938, 1266
BslFI GGGAC 1 cut(s) 1248
BslI CCNNNNNNNGG 1 cut(s) 1298
BsmAI GTCTC 3 cut(s) 49, 88, 457
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 1248
BsnI GGCC 2 cut(s) 231, 852
Bso31I GGTCTC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 1071
Bsp1407I TGTACA 1 cut(s) 1229
Bsp143I GATC 4 cut(s) 99, 184, 223, 1288
Bsp1720I GCTNAGC 1 cut(s) 649
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 3 cut(s) 189, 787, 1211
BspANI GGCC 2 cut(s) 231, 852
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 142
BspPI GGATC 2 cut(s) 179, 1283
BspQI GCTCTTC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 674
BsrGI TGTACA 1 cut(s) 1229
BsrI ACTGG 4 cut(s) 206, 375, 562, 694
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 2 cut(s) 409, 1038
BssMI GATC 4 cut(s) 99, 184, 223, 1288
BssT1I CCWWGG 1 cut(s) 1038
Bst2UI CCWGG 2 cut(s) 999, 1054
Bst4CI ACNGT 4 cut(s) 78, 124, 413, 436
Bst6I CTCTTC 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 1229
BstC8I GCNNGC 1 cut(s) 880
BstDEI CTNAG 5 cut(s) 566, 606, 649, 798, 1058
BstDSI CCRYGG 2 cut(s) 409, 1038
BstF5I GGATG 3 cut(s) 98, 946, 1010
BstKTI GATC 4 cut(s) 102, 187, 226, 1291
BstMAI GTCTC 3 cut(s) 49, 88, 457
BstMBI GATC 4 cut(s) 99, 184, 223, 1288
BstMWI GCNNNNNNNGC 2 cut(s) 1040, 1043
BstNI CCWGG 2 cut(s) 999, 1054
BstNSI RCATGY 1 cut(s) 848
BstPAI GACNNNNGTC 1 cut(s) 282
BstSCI CCNGG 3 cut(s) 997, 1052, 1264
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 1021, 1030
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuRI GGCC 2 cut(s) 231, 852
BtgI CCRYGG 2 cut(s) 409, 1038
BtgZI GCGATG 1 cut(s) 979
BtrI CACGTC 1 cut(s) 1262
BtsCI GGATG 3 cut(s) 98, 946, 1010
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 441, 519
Cac8I GCNNGC 1 cut(s) 880
CciI TCATGA 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 674
Csp6I GTAC 2 cut(s) 958, 1230
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 6 cut(s) 182, 614, 845, 854, 1039, 1185
CviQI GTAC 2 cut(s) 958, 1230
DdeI CTNAG 5 cut(s) 566, 606, 649, 798, 1058
DpnI GATC 4 cut(s) 101, 186, 225, 1290
DpnII GATC 4 cut(s) 99, 184, 223, 1288
DriI GACNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 850
Eam1104I CTCTTC 1 cut(s) 497
Eam1105I GACNNNNNGTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 1038
Eco147I AGGCCT 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 9, 1293
EcoRI GAATTC 1 cut(s) 869
EcoRII CCWGG 2 cut(s) 997, 1052
EcoRV GATATC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 1038
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 6 cut(s) 185, 617, 848, 857, 1042, 1188
FaqI GGGAC 1 cut(s) 1248
FatI CATG 6 cut(s) 181, 613, 844, 853, 1038, 1184
FauI CCCGC 1 cut(s) 196
Fnu4HI GCNGC 3 cut(s) 1035, 1044, 1212
FokI GGATG 3 cut(s) 105, 953, 1017
Fsp4HI GCNGC 3 cut(s) 1035, 1044, 1212
FspBI CTAG 4 cut(s) 227, 597, 623, 896
GluI GCNGC 3 cut(s) 1035, 1044, 1212
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 2 cut(s) 231, 852
HapII CCGG 3 cut(s) 675, 938, 1266
Hin1II CATG 6 cut(s) 185, 617, 848, 857, 1042, 1188
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HindIII AAGCTT 2 cut(s) 375, 973
HinfI GANTC 8 cut(s) 14, 292, 304, 323, 580, 887, 1161, 1218
HpaI GTTAAC 1 cut(s) 1029
HpaII CCGG 3 cut(s) 675, 938, 1266
HphI GGTGA 2 cut(s) 266, 398
Hpy166II GTNNAC 3 cut(s) 1010, 1029, 1232
Hpy188I TCNGA 2 cut(s) 109, 1182
Hpy188III TCNNGA 4 cut(s) 182, 287, 532, 577
Hpy8I GTNNAC 3 cut(s) 1010, 1029, 1232
Hpy99I CGWCG 1 cut(s) 956
HpyAV CCTTC 5 cut(s) 199, 438, 700, 1023, 1317
HpyCH4III ACNGT 4 cut(s) 78, 124, 413, 436
HpyCH4IV ACGT 2 cut(s) 450, 1261
HpyCH4V TGCA 4 cut(s) 236, 242, 479, 718
HpyF10VI GCNNNNNNNGC 2 cut(s) 1040, 1043
HpyF3I CTNAG 5 cut(s) 566, 606, 649, 798, 1058
HpySE526I ACGT 2 cut(s) 450, 1261
Hsp92II CATG 6 cut(s) 185, 617, 848, 857, 1042, 1188
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 4 cut(s) 99, 184, 223, 1288
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 1 cut(s) 1066
Lsp1109I GCAGC 2 cut(s) 1021, 1030
LweI GCATC 2 cut(s) 229, 733
MaeI CTAG 4 cut(s) 227, 597, 623, 896
MaeII ACGT 2 cut(s) 450, 1261
MaeIII GTNAC 3 cut(s) 58, 779, 884
MalI GATC 4 cut(s) 101, 186, 225, 1290
MbiI CCGCTC 1 cut(s) 191
MboI GATC 4 cut(s) 99, 184, 223, 1288
MboII GAAGA 9 cut(s) 122, 164, 372, 375, 484, 557, 565, 986, 1041
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 1071
MlyI GAGTC 2 cut(s) 574, 881
MnlI CCTC 7 cut(s) 110, 130, 619, 638, 748, 859, 1110
Mph1103I ATGCAT 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 1124
MseI TTAA 3 cut(s) 309, 1019, 1028
MspA1I CMGCKG 1 cut(s) 1034
MspI CCGG 3 cut(s) 675, 938, 1266
MspR9I CCNGG 3 cut(s) 999, 1054, 1266
MvaI CCWGG 2 cut(s) 999, 1054
MwoI GCNNNNNNNGC 2 cut(s) 1040, 1043
NciI CCSGG 1 cut(s) 1266
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 4 cut(s) 99, 184, 223, 1288
NlaIII CATG 6 cut(s) 185, 617, 848, 857, 1042, 1188
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 779, 884
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 848
PagI TCATGA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 231
PciI ACATGT 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 6 cut(s) 14, 292, 304, 323, 1161, 1218
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 3 cut(s) 1036, 1045, 1213
PleI GAGTC 2 cut(s) 574, 881
PpsI GAGTC 2 cut(s) 574, 881
PscI ACATGT 1 cut(s) 844
PshAI GACNNNNGTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 997, 1052
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 2 cut(s) 997, 1052
PspN4I GGNNCC 1 cut(s) 142
PsuI RGATCY 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 2 cut(s) 959, 1231
RsaNI GTAC 2 cut(s) 958, 1230
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 3 cut(s) 309, 1019, 1028
SatI GCNGC 3 cut(s) 1035, 1044, 1212
Sau3AI GATC 4 cut(s) 99, 184, 223, 1288
SchI GAGTC 2 cut(s) 574, 881
ScrFI CCNGG 3 cut(s) 999, 1054, 1266
SduI GDGCHC 1 cut(s) 1071
SfaNI GCATC 2 cut(s) 229, 733
SfcI CTRYAG 1 cut(s) 87
SmlI CTYRAG 2 cut(s) 469, 752
SmoI CTYRAG 2 cut(s) 469, 752
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 189, 787, 1211
SspI AATATT 3 cut(s) 33, 1112, 1193
SspMI CTAG 4 cut(s) 227, 597, 623, 896
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 997, 1052, 1264
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 4 cut(s) 78, 124, 413, 436
TaiI ACGT 2 cut(s) 453, 1264
TaqI TCGA 4 cut(s) 442, 657, 704, 1164
TatI WGTACW 2 cut(s) 957, 1229
TauI GCSGC 1 cut(s) 1214
TfiI GAWTC 6 cut(s) 14, 292, 304, 323, 1161, 1218
Tru1I TTAA 3 cut(s) 309, 1019, 1028
Tru9I TTAA 3 cut(s) 309, 1019, 1028
TscAI CASTG 2 cut(s) 441, 526
TseFI GTSAC 2 cut(s) 779, 884
TseI GCWGC 2 cut(s) 1034, 1043
Tsp45I GTSAC 2 cut(s) 779, 884
TspDTI ATGAA 5 cut(s) 170, 558, 630, 870, 967
TspRI CASTG 2 cut(s) 441, 526
XapI RAATTY 4 cut(s) 65, 767, 869, 1123
XceI RCATGY 1 cut(s) 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 4 cut(s) 227, 597, 623, 896
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.