Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0000546

Physical Location & Seq

Genomic Coordinates Forward (+)

27437459 .. 27446024

Gene Structure 8566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25760.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3765 bp

ATGCTATCACCGGTCGTCGACTCCCCGTCGGCGGTGGGGTCCACTGCTGCTGGTGCTACTCATTCCTCCGCGGATAGGATTGTGGTGGAGCCTGTGGCTGGGGTGATCCAAGATACGATATATGTGACAGTGGGAGCTTGTGTGAAGGAGAGCAAGTCGCATCTGTTATGGACATTGCATAACTCAGGAGGGAACAGGGTTTGCATTATTCATGTTCATCAGCCTGCACAGAAGATTCCAACGATGATGGGAAGCTTCCCAGCAAGCCAACTGAACCATCAGGTAATCAGAGATTATCGTCAAATCGAAAGGCAAAAGATGCAGAAGATCCTGGAAGAATACCTTCATATTTGTCGTCAAATGGGGGTTCGAGCAGAAATATTACATATTGAAATGGACTCTATTGAGAAGGGAATAGTGGAACTTATTTCTCAGCACGGGATCAGGAAGCTTGTTATGGGAACAGGAGCAGTTAAAACCCATGGAGCTCGGAAAATGAGGGGCCTCATGTCTAAGAAAGCCATATACGTGCGTGAGCACGCACCTGTGTCCTGTCACATACAATTTGTATACCAGGGGGGGTGCCTTATACAAACCAGGGAAGCGAAATCAGATGCAGTTGATGCACAAGTGGCATTACTGCCACCAAGGCCAAGCACCGACACTGGACAATCACCACTTCATTTAAGATCAAGGTCTTCTGTGACATATGGGCAGGATGATCTACCGTCACTCAGCAACCCAGCTCAACGCGTACGTTTGTTCGGCCCGGTAAGATTGATAAAAGGTGACAAATATGGAGGAAGCACAACAGATGTTCCCGAAGGGTTTATAACTCCATCAAGCCGCATGTTGGAAGCAGAAGGTAGTGCTGATGAATACGCAATAAGGAGGAGTGCTTCTTTATCTTCGATACAGGTTGAGAGGACTGACAAAAGTGAAAATGGTTCAGGAAACTCGAGTTATCTTACTCGTCTCAAAGATCTTAATCATTCATCCCCTCCCAGTCTATTCAGTCCGCCAGAGTCTCTGGAGGATGCAAATTTCGAACAGAAGCTTAGGCAGGTTCAAGATGAGCATGAGACTTTGCCTCACCATTTGAAAAGGTTATTTCTGTATTGTTCACTCTTTCCCTTTGGTTATGAATTTAGTAAAGATGGGTTGGTTCAGTTATGGATAGCTGAAGGCTTTATTAGAGAGAGGCAGAGGGAGAGAATGGAAGATACTGCTACTGAGCATTTTAATTCCTTGGAGAAGGAAGGCTTCTTTGTGTTTTCTAGATGTGATTTCACTACGGATTTTGATTCAATGCTCTCGACTACGACAGATCATCCCAGCAACTTCTTGTACAAAGTAAATCCTAGGAAGCATTCATTATTGGAGAACACCATTTCTTCAGGTAATTATTTCAAAGCAGTGGATGGTAAGTTAGATGGTGCCTCTCACATGACTAGACATTTGTCTTTGATTGGTGAGGATATTGATGGGGTGTCTTTTGGGATTCTTCAAAACTTCAAGCATTTGCGCTCACTGCATATGCTCTCTTGTCGTGGGTCTTCCTTGAAACAAGTCCCTCGTGATTTGTGTTTCACCTTAACCTTCTTGAAAACCTTAAATTTGAGTGGGACATTGATTTCTGAATTGCCTAGTTCCATCGGGAATGTAAAGTCATTACGATATATTGATGCCTCACATACACCAATTGCACGGCTACCTGAATCAATAGATTCTCTTTACAATTTACAAACGATAAAGCTCAGAGGTTGCTCAAACTTTGTTCAGTTGCCAAAAGGTATGAAGAAGCTAACAAATCTCCGCCATATTGATCTTGATATCATTCGGCAATTGGATTCCTTGCCAGCACATTTAGGGAATTTGAGTACCCTTCAAACTCTGTCAGCATTTCTTGTTGGTAGAGATGATGGGTGCCATGTTGGAGAGTTGAAGAATTTGAATGATCTCAAAGGAGCGCTTAACATCTCAAGGCTTGAAAATGTGGTCAGTAAGAAGGAGGCTGAGGAAGCTGCTTTGATTGATAAGAAATGTCTAGACAGGTTGGAGCTTCGATGGAGTAATATGTTTGTTGAGTATGCCAAGTTAGAAGAGGAAATACTAGAATGTCTTCAACCACATTTTGGCCTTAAAGAGTTGCAAATACAACAGTACAGTGGCTCAGTACTTCCAACTTGGATTGGCAATCGCGCTTTTGCTGACCTTGTTGCCATTACCCTCTATAGATGCAGGAATTGTAAACTTCTCCCATGTATGGGACAATTGCCAGCACTCAAGTCTCTTTCAATTATAGAGATGAATGGGGTGAAATATATTGATCATCAGTTCCTCAGGAATGGCCCATGTCTCGAGTATGCATTTCCAAAACTAGAGAGACTAGAAGTTGATATCATGCTCAAACTGAAAGTGTGGAAGCACATACGAATAGGTGACTTCCCTTCCCTGGTTAAACTCATAGTGGACTCCTGCCCAGAACTTGTCAGACTTCCACCACTGGCATGCCTCCCGTCCTTAAAGCACTTGGAGTTGAGACGATGCCCGAAGCTTCTCTCTTTGCCCAGTGACGGACTGCCACCTTCTCTTGAGTCTTTCTTATTAGTGGAGTGCCTTGAGCTGAAAGAATGGTGCCTGAAGAGTGACCATTGGAGCACGTTATGTCATATCTCCAGCATATGGTTTGATCATGAGGAGGTGAAGGGGTTTGAGCTGGACTGCACACCTCTCTGGGGTTCTGTTTCAATCTGTGGAAGAAGACCAGTAATGGAAGATGCAATTGCTGTAGTACCTCGGTTCATTAATATTCCAAGCACAATGCTTATTGGCAGTCATGCATACACTGGAATGAGCCCAAGTTTGAACCACCTAACAAGTCATTTTTTTGGCGTTTATGATGGGCATGGAGGACCTCAGGTTGCTGAGTATTGTTGTGAGCGCCTTCATTTAGCTTTGGCTGACGAACTTCAAATTATTGAGGATGATTTGACTGATGGAATAATGAGAGAAACTCAGCATGTGAAGGGGGAGAAAGCATTCACTATTGAGGATGATTTGACTGATGGAATAATGAGAGAAACTCAGCAGGTGAAGTGGGAGAAAGCATTCACTAGTTGCTTCCGGAGAGTAGATGATGAAGTCAGCAGATGCATCACCGGAAGTAACGAGGATGCCTCCGGGGCCAACTCTGATCAACATATTGCCCCAGAGACCGTAGGATCTACAGCTGTGGTTGCATTAGTCTGTTCTTCCCATATTATAGTTGCGAACTGTGGTGACTCGAGAGCGATTCTTTGTCGTGGCAAAGAACCAGTACCATTATCAGTAGATCATAAAGCAAACAGAGAAGATGAATGTGCAAGAATTGAGGCATCTGGAGGGAAGATCATACAGTGGAATGGACCTCGTGTGTTTGGAGTTCTTGCAATGTCAAGGTCCATTGGAGATGGATATTTGAAACCCTGGATAATTCCAGATCCAGAAGTGATGATTGTCCCTAGAACTAGAGATGATGAATTCCTCATTTTGGCTAGTGATGGATTATGGGATGTTATGACGAACAAGGAAGCTTGTAAAGTGGCTCGAAGGCGTATACGGCTGTGGCACAAAAAGAACAGGGTTACTCTGGCGGAAAGAGGCATAGGAGTTGATCCGGCAGCGCAAGAAGCGGCTTCATATCTTTCAACTCTTGCCTTTCAAAAAGGAAGCACAGACAATATATCTGTGATTCTGGTGGACATGAGAGCTCAAAGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010035 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0022622 GO:0023051 GO:0023057 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

1255

Amino Acids

140.15

Weight (kDa)

6.6

Isoelectric Point (pI)

46.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_DRP	PF23559	245 - 293	9.7e-14	Disease resistance protein Winged helix domain
LRR_14	PF23598	365 - 427	1e-05	Leucine-rich repeat region
LRR_14	PF23598	421 - 671	1e-23	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	515 - 639	3.8e-37	R13L1/DRL21 LRRs
PP2C	PF00481	806 - 1111	1.2e-58	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica	MD14G1049300.v1.1
rosa_chinensis	RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata	RLG00000001164 RLG00000013941
rosa_multiflora	Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii	Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa	Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha	Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana	Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	833
AarI	CACCTGC	1 cut(s)	3082
Acc36I	ACCTGC	2 cut(s)	1054, 3082
AccB1I	GGYRCC	4 cut(s)	582, 1436, 1926, 2637
AccB7I	CCANNNNNTGG	2 cut(s)	2135, 2685
AccI	GTMKAC	3 cut(s)	18, 570, 3598
AccII	CGCG	3 cut(s)	71, 753, 2202
AccIII	TCCGGA	1 cut(s)	3126
AciI	CCGC	8 cut(s)	32, 69, 71, 847, 1019, 1816, 3635, 3674
AclWI	GGATC	6 cut(s)	100, 322, 449, 3232, 3476, 3650
AcsI	RAATTY	6 cut(s)	1042, 1145, 1615, 1873, 1948, 3521
AcuI	CTGAAG	3 cut(s)	1203, 1380, 2663
AfaI	GTAC	7 cut(s)	756, 1349, 1882, 2165, 2178, 2796, 3321
AfeI	AGCGCT	1 cut(s)	1971
AflIII	ACRYGT	1 cut(s)	751
AgeI	ACCGGT	1 cut(s)	10
AhdI	GACNNNNNGTC	1 cut(s)	25
AhlI	ACTAGT	1 cut(s)	3116
AjnI	CCWGG	5 cut(s)	330, 573, 596, 2454, 3467
Alw21I	GWGCWC	4 cut(s)	490, 540, 2663, 3754
Alw26I	GTCTC	8 cut(s)	980, 1032, 1076, 2295, 2363, 2380, 2536, 3209
AlwI	GGATC	6 cut(s)	100, 322, 449, 3232, 3476, 3650
Ama87I	CYCGRG	3 cut(s)	958, 2360, 3286
Aor13HI	TCCGGA	1 cut(s)	3126
Aor51HI	AGCGCT	1 cut(s)	1971
AoxI	GGCC	6 cut(s)	502, 650, 766, 2137, 2350, 3186
ApeKI	GCWGC	3 cut(s)	47, 2024, 3662
ApoI	RAATTY	6 cut(s)	1042, 1145, 1615, 1873, 1948, 3521
ArsI	GACNNNNNNTTYG	2 cut(s)	1441, 1473
AseI	ATTAAT	1 cut(s)	2808
AsiGI	ACCGGT	1 cut(s)	10
Asp700I	GAANNNNTTC	4 cut(s)	342, 2121, 3041, 3110
AspA2I	CCTAGG	1 cut(s)	1361
AspLEI	GCGC	5 cut(s)	1527, 1972, 2204, 2946, 3667
AspS9I	GGNCC	8 cut(s)	39, 502, 767, 2351, 2915, 3186, 3407, 3441
AsuC2I	CCSGG	2 cut(s)	770, 3184
AsuII	TTCGAA	1 cut(s)	1047
AvaI	CYCGRG	3 cut(s)	958, 2360, 3286
AvaII	GGWCC	4 cut(s)	39, 2915, 3407, 3441
AvrII	CCTAGG	1 cut(s)	1361
AxyI	CCTNAGG	2 cut(s)	2342, 2919
BanI	GGYRCC	4 cut(s)	582, 1436, 1926, 2637
BanII	GRGCYC	3 cut(s)	490, 2861, 3754
BarI	GAAGNNNNNNTAC	2 cut(s)	2094, 2126
BauI	CACGAG	2 cut(s)	1575, 3411
BbsI	GAAGAC	4 cut(s)	690, 1548, 2114, 2770
Bbv12I	GWGCWC	4 cut(s)	490, 540, 2663, 3754
BbvCI	CCTCAGC	1 cut(s)	2016
BbvI	GCAGC	3 cut(s)	34, 2011, 3674
BceAI	ACGGC	2 cut(s)	1724, 3617
BciT130I	CCWGG	5 cut(s)	332, 575, 598, 2456, 3469
BclI	TGATCA	3 cut(s)	2329, 2692, 3196
BcnI	CCSGG	2 cut(s)	770, 3184
BcoDI	GTCTC	8 cut(s)	980, 1032, 1076, 2295, 2363, 2380, 2536, 3209
BcuI	ACTAGT	1 cut(s)	3116
BfmI	CTRYAG	3 cut(s)	2233, 2790, 3228
BfoI	RGCGCY	2 cut(s)	1973, 2947
BfuAI	ACCTGC	2 cut(s)	1054, 3082
BglI	GCCNNNNNGGC	2 cut(s)	649, 3185
BglII	AGATCT	1 cut(s)	982
BisI	GCNGC	5 cut(s)	48, 847, 2025, 3663, 3675
BlnI	CCTAGG	1 cut(s)	1361
BlsI	GCNGC	5 cut(s)	49, 848, 2026, 3664, 3676
BmcAI	AGTACT	1 cut(s)	2178
Bme1390I	CCNGG	7 cut(s)	332, 575, 598, 770, 2456, 3184, 3469
Bme18I	GGWCC	4 cut(s)	39, 2915, 3407, 3441
BmeRI	GACNNNNNGTC	1 cut(s)	25
BmeT110I	CYCGRG	3 cut(s)	958, 2360, 3286
BmgT120I	GGNCC	8 cut(s)	39, 502, 767, 2351, 2915, 3186, 3407, 3441
BmiI	GGNNCC	8 cut(s)	40, 90, 503, 584, 1438, 1928, 2639, 3187
BmrFI	CCNGG	7 cut(s)	332, 575, 598, 770, 2456, 3184, 3469
BmrI	ACTGGG	2 cut(s)	999, 2565
BmuI	ACTGGG	2 cut(s)	999, 2565
BoxI	GACNNNNGTC	1 cut(s)	1459
BpiI	GAAGAC	4 cut(s)	690, 1548, 2114, 2770
BplI	GAGNNNNNCTC	6 cut(s)	3001, 3033, 3070, 3102, 3164, 3196
BpmI	CTGGAG	3 cut(s)	1052, 2662, 3402
Bpu10I	CCTNAGC	2 cut(s)	1058, 2016
Bpu14I	TTCGAA	1 cut(s)	1047
BpuEI	CTTGAG	4 cut(s)	1966, 2270, 2615, 2642
BpuMI	CCSGG	2 cut(s)	770, 3184
BsaAI	YACGTR	1 cut(s)	529
BsaBI	GATNNNNATC	1 cut(s)	987
BsaI	GGTCTC	1 cut(s)	3209
BsaWI	WCCGGW	3 cut(s)	10, 3126, 3161
BsaXI	ACNNNNNCTCC	4 cut(s)	126, 140, 156, 170
Bse118I	RCCGGY	1 cut(s)	10
Bse1I	ACTGG	7 cut(s)	670, 1005, 2511, 2571, 2768, 2854, 3317
Bse21I	CCTNAGG	2 cut(s)	2342, 2919
Bse3DI	GCAATG	2 cut(s)	173, 3438
Bse8I	GATNNNNATC	1 cut(s)	987
BseAI	TCCGGA	1 cut(s)	3126
BseBI	CCWGG	5 cut(s)	332, 575, 598, 2456, 3469
BseGI	GGATG	9 cut(s)	724, 995, 1042, 1330, 1426, 2992, 3061, 3181, 3559
BseJI	GATNNNNATC	1 cut(s)	987
BseMI	GCAATG	2 cut(s)	173, 3438
BseNI	ACTGG	7 cut(s)	670, 1005, 2511, 2571, 2768, 2854, 3317
BseRI	GAGGAG	2 cut(s)	907, 2714
BseXI	GCAGC	3 cut(s)	34, 2011, 3674
BseYI	CCCAGC	4 cut(s)	98, 259, 742, 1334
BsgI	GTGCAG	2 cut(s)	210, 2710
Bsh1236I	CGCG	3 cut(s)	71, 753, 2202
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	6 cut(s)	504, 652, 768, 2139, 2352, 3188
BshNI	GGYRCC	4 cut(s)	582, 1436, 1926, 2637
BshTI	ACCGGT	1 cut(s)	10
BsiEI	CGRYCG	1 cut(s)	15
BsiHKAI	GWGCWC	4 cut(s)	490, 540, 2663, 3754
BsiHKCI	CYCGRG	3 cut(s)	958, 2360, 3286
BsiSI	CCGG	6 cut(s)	11, 770, 3127, 3162, 3183, 3659
BsiWI	CGTACG	1 cut(s)	754
BslFI	GGGAC	4 cut(s)	1556, 1639, 2283, 3485
BsmAI	GTCTC	8 cut(s)	980, 1032, 1076, 2295, 2363, 2380, 2536, 3209
BsmBI	CGTCTC	2 cut(s)	980, 2536
BsmFI	GGGAC	4 cut(s)	1556, 1639, 2283, 3485
BsmI	GAATGC	3 cut(s)	1369, 3041, 3110
BsnI	GGCC	6 cut(s)	504, 652, 768, 2139, 2352, 3188
Bso31I	GGTCTC	1 cut(s)	3209
BsoBI	CYCGRG	3 cut(s)	958, 2360, 3286
Bsp119I	TTCGAA	1 cut(s)	1047
Bsp1286I	GDGCHC	5 cut(s)	490, 540, 2663, 2861, 3754
Bsp13I	TCCGGA	1 cut(s)	3126
Bsp1407I	TGTACA	1 cut(s)	1347
Bsp19I	CCATGG	1 cut(s)	481
BspACI	CCGC	8 cut(s)	32, 69, 71, 847, 1019, 1816, 3635, 3674
BspANI	GGCC	6 cut(s)	504, 652, 768, 2139, 2352, 3188
BspEI	TCCGGA	1 cut(s)	3126
BspFNI	CGCG	3 cut(s)	71, 753, 2202
BspHI	TCATGA	1 cut(s)	2695
BspLI	GGNNCC	8 cut(s)	40, 90, 503, 584, 1438, 1928, 2639, 3187
BspMI	ACCTGC	2 cut(s)	1054, 3082
BspPI	GGATC	6 cut(s)	100, 322, 449, 3232, 3476, 3650
BspT104I	TTCGAA	1 cut(s)	1047
BspT107I	GGYRCC	4 cut(s)	582, 1436, 1926, 2637
BspTNI	GGTCTC	1 cut(s)	3209
BsrDI	GCAATG	2 cut(s)	173, 3438
BsrFI	RCCGGY	1 cut(s)	10
BsrGI	TGTACA	1 cut(s)	1347
BsrI	ACTGG	7 cut(s)	670, 1005, 2511, 2571, 2768, 2854, 3317
BssAI	RCCGGY	1 cut(s)	10
BssNAI	GTATAC	2 cut(s)	571, 3599
BssSI	CACGAG	2 cut(s)	1575, 3411
BssT1I	CCWWGG	4 cut(s)	481, 647, 1248, 1361
Bst1107I	GTATAC	2 cut(s)	571, 3599
Bst2BI	CACGAG	2 cut(s)	1575, 3411
Bst2UI	CCWGG	5 cut(s)	332, 575, 598, 2456, 3469
Bst4CI	ACNGT	7 cut(s)	130, 729, 2163, 2168, 3220, 3278, 3399
Bst6I	CTCTTC	2 cut(s)	2097, 2639
BstAPI	GCANNNNNTGC	2 cut(s)	319, 623
BstAUI	TGTACA	1 cut(s)	1347
BstBAI	YACGTR	1 cut(s)	529
BstBI	TTCGAA	1 cut(s)	1047
BstC8I	GCNNGC	6 cut(s)	225, 265, 540, 1860, 2280, 2512
BstDSI	CCRYGG	2 cut(s)	69, 481
BstF5I	GGATG	9 cut(s)	724, 995, 1042, 1330, 1426, 2992, 3061, 3181, 3559
BstFNI	CGCG	3 cut(s)	71, 753, 2202
BstH2I	RGCGCY	2 cut(s)	1973, 2947
BstHHI	GCGC	5 cut(s)	1527, 1972, 2204, 2946, 3667
BstMAI	GTCTC	8 cut(s)	980, 1032, 1076, 2295, 2363, 2380, 2536, 3209
BstMCI	CGRYCG	1 cut(s)	15
BstNI	CCWGG	5 cut(s)	332, 575, 598, 2456, 3469
BstNSI	RCATGY	3 cut(s)	853, 2514, 3026
BstPAI	GACNNNNGTC	1 cut(s)	1459
BstSCI	CCNGG	7 cut(s)	330, 573, 596, 768, 2454, 3182, 3467
BstSFI	CTRYAG	3 cut(s)	2233, 2790, 3228
BstUI	CGCG	3 cut(s)	71, 753, 2202
BstV1I	GCAGC	3 cut(s)	34, 2011, 3674
BstV2I	GAAGAC	4 cut(s)	690, 1548, 2114, 2770
BstX2I	RGATCY	4 cut(s)	327, 982, 3224, 3481
BstYI	RGATCY	4 cut(s)	327, 982, 3224, 3481
BstZ17I	GTATAC	2 cut(s)	571, 3599
Bsu36I	CCTNAGG	2 cut(s)	2342, 2919
BsuRI	GGCC	6 cut(s)	504, 652, 768, 2139, 2352, 3188
BtgI	CCRYGG	2 cut(s)	69, 481
BtsCI	GGATG	9 cut(s)	724, 995, 1042, 1330, 1426, 2992, 3061, 3181, 3559
BtsI	GCAGTG	3 cut(s)	42, 1422, 1529
BveI	ACCTGC	2 cut(s)	1054, 3082
Cac8I	GCNNGC	6 cut(s)	225, 265, 540, 1860, 2280, 2512
CciI	TCATGA	1 cut(s)	2695
CfoI	GCGC	5 cut(s)	1527, 1972, 2204, 2946, 3667
Cfr10I	RCCGGY	1 cut(s)	10
Cfr13I	GGNCC	8 cut(s)	39, 502, 767, 2351, 2915, 3186, 3407, 3441
Cfr42I	CCGCGG	1 cut(s)	72
Csp6I	GTAC	7 cut(s)	755, 1348, 1881, 2164, 2177, 2795, 3320
CspAI	ACCGGT	1 cut(s)	10
CviQI	GTAC	7 cut(s)	755, 1348, 1881, 2164, 2177, 2795, 3320
DriI	GACNNNNNGTC	1 cut(s)	25
Eam1104I	CTCTTC	2 cut(s)	2097, 2639
Eam1105I	GACNNNNNGTC	1 cut(s)	25
EarI	CTCTTC	2 cut(s)	2097, 2639
EciI	GGCGGA	3 cut(s)	1008, 1805, 3650
Ecl136II	GAGCTC	2 cut(s)	488, 3752
Eco130I	CCWWGG	4 cut(s)	481, 647, 1248, 1361
Eco24I	GRGCYC	3 cut(s)	490, 2861, 3754
Eco31I	GGTCTC	1 cut(s)	3209
Eco32I	GATATC	2 cut(s)	1834, 2401
Eco47I	GGWCC	4 cut(s)	39, 2915, 3407, 3441
Eco47III	AGCGCT	1 cut(s)	1971
Eco53kI	GAGCTC	2 cut(s)	488, 3752
Eco57I	CTGAAG	3 cut(s)	1203, 1380, 2663
Eco81I	CCTNAGG	2 cut(s)	2342, 2919
Eco88I	CYCGRG	3 cut(s)	958, 2360, 3286
EcoICRI	GAGCTC	2 cut(s)	488, 3752
EcoO109I	RGGNCCY	2 cut(s)	502, 2915
EcoRI	GAATTC	1 cut(s)	3521
EcoRII	CCWGG	5 cut(s)	330, 573, 596, 2454, 3467
EcoRV	GATATC	2 cut(s)	1834, 2401
EcoT14I	CCWWGG	4 cut(s)	481, 647, 1248, 1361
EcoT22I	ATGCAT	3 cut(s)	2371, 2845, 3158
EcoT38I	GRGCYC	3 cut(s)	490, 2861, 3754
ErhI	CCWWGG	4 cut(s)	481, 647, 1248, 1361
Esp3I	CGTCTC	2 cut(s)	980, 2536
FalI	AAGNNNNNCTT	6 cut(s)	327, 359, 1247, 1279, 1956, 1988
FaqI	GGGAC	4 cut(s)	1556, 1639, 2283, 3485
FauNDI	CATATG	3 cut(s)	709, 1536, 2684
FbaI	TGATCA	3 cut(s)	2329, 2692, 3196
FblI	GTMKAC	3 cut(s)	18, 570, 3598
Fnu4HI	GCNGC	5 cut(s)	48, 847, 2025, 3663, 3675
FokI	GGATG	9 cut(s)	731, 982, 1049, 1317, 1433, 2999, 3068, 3188, 3566
FriOI	GRGCYC	3 cut(s)	490, 2861, 3754
Fsp4HI	GCNGC	5 cut(s)	48, 847, 2025, 3663, 3675
GlaI	GCGC	5 cut(s)	1526, 1971, 2203, 2945, 3666
GluI	GCNGC	5 cut(s)	48, 847, 2025, 3663, 3675
GsaI	CCCAGC	4 cut(s)	102, 263, 746, 1338
GsuI	CTGGAG	3 cut(s)	1052, 2662, 3402
HaeII	RGCGCY	2 cut(s)	1973, 2947
HaeIII	GGCC	6 cut(s)	504, 652, 768, 2139, 2352, 3188
HapII	CCGG	6 cut(s)	11, 770, 3127, 3162, 3183, 3659
HhaI	GCGC	5 cut(s)	1527, 1972, 2204, 2946, 3667
Hin6I	GCGC	5 cut(s)	1525, 1970, 2202, 2944, 3665
HinP1I	GCGC	5 cut(s)	1525, 1970, 2202, 2944, 3665
HincII	GTYRAC	1 cut(s)	19
HindII	GTYRAC	1 cut(s)	19
HindIII	AAGCTT	5 cut(s)	253, 449, 1055, 2555, 3573
HpaII	CCGG	6 cut(s)	11, 770, 3127, 3162, 3183, 3659
Hpy166II	GTNNAC	8 cut(s)	19, 42, 571, 1124, 2252, 2473, 3599, 3742
Hpy188I	TCNGA	7 cut(s)	290, 492, 613, 1639, 1760, 2495, 3196
Hpy8I	GTNNAC	8 cut(s)	19, 42, 571, 1124, 2252, 2473, 3599, 3742
Hpy99I	CGWCG	2 cut(s)	20, 31
HpyCH4III	ACNGT	7 cut(s)	130, 729, 2163, 2168, 3220, 3278, 3399
HpyCH4IV	ACGT	3 cut(s)	528, 757, 2663
HpySE526I	ACGT	3 cut(s)	528, 757, 2663
HspAI	GCGC	5 cut(s)	1525, 1970, 2202, 2944, 3665
Kpn2I	TCCGGA	1 cut(s)	3126
Ksp22I	TGATCA	3 cut(s)	2329, 2692, 3196
KspI	CCGCGG	1 cut(s)	72
LmnI	GCTCC	7 cut(s)	88, 134, 467, 485, 1967, 2059, 2658
Lsp1109I	GCAGC	3 cut(s)	34, 2011, 3674
MaeII	ACGT	3 cut(s)	528, 757, 2663
MfeI	CAATTG	4 cut(s)	1701, 1844, 2273, 2784
MflI	RGATCY	4 cut(s)	327, 982, 3224, 3481
MhlI	GDGCHC	5 cut(s)	490, 540, 2663, 2861, 3754
MluI	ACGCGT	1 cut(s)	751
MlyI	GAGTC	6 cut(s)	14, 392, 1034, 2468, 2606, 3278
MmeI	TCCRAC	5 cut(s)	263, 834, 1915, 2037, 2207
Mph1103I	ATGCAT	3 cut(s)	2371, 2845, 3158
MroI	TCCGGA	1 cut(s)	3126
MroXI	GAANNNNTTC	4 cut(s)	342, 2121, 3041, 3110
MslI	CAYNNNNRTG	3 cut(s)	527, 2509, 2852
MspA1I	CMGCKG	2 cut(s)	71, 3233
MspI	CCGG	6 cut(s)	11, 770, 3127, 3162, 3183, 3659
MspR9I	CCNGG	7 cut(s)	332, 575, 598, 770, 2456, 3184, 3469
MunI	CAATTG	4 cut(s)	1701, 1844, 2273, 2784
Mva1269I	GAATGC	3 cut(s)	1369, 3041, 3110
MvaI	CCWGG	5 cut(s)	332, 575, 598, 2456, 3469
MvnI	CGCG	3 cut(s)	71, 753, 2202
NciI	CCSGG	2 cut(s)	770, 3184
NcoI	CCATGG	1 cut(s)	481
NdeI	CATATG	3 cut(s)	709, 1536, 2684
NlaIV	GGNNCC	8 cut(s)	40, 90, 503, 584, 1438, 1928, 2639, 3187
NmuCI	GTSAC	9 cut(s)	124, 554, 703, 729, 788, 2441, 2573, 2648, 3281
NsiI	ATGCAT	3 cut(s)	2371, 2845, 3158
NspI	RCATGY	3 cut(s)	853, 2514, 3026
NspV	TTCGAA	1 cut(s)	1047
PaeI	GCATGC	1 cut(s)	2514
PaeR7I	CTCGAG	3 cut(s)	958, 2360, 3286
PagI	TCATGA	1 cut(s)	2695
PaqCI	CACCTGC	1 cut(s)	3082
PctI	GAATGC	3 cut(s)	1369, 3041, 3110
PdmI	GAANNNNTTC	4 cut(s)	342, 2121, 3041, 3110
PfeI	GAWTC	8 cut(s)	235, 1304, 1501, 1718, 1727, 1850, 3295, 3733
Pfl23II	CGTACG	1 cut(s)	754
PflMI	CCANNNNNTGG	2 cut(s)	2135, 2685
PfoI	TCCNGGA	1 cut(s)	330
PinAI	ACCGGT	1 cut(s)	10
PkrI	GCNGC	5 cut(s)	49, 848, 2026, 3664, 3676
PleI	GAGTC	6 cut(s)	14, 392, 1033, 2468, 2605, 3278
PpsI	GAGTC	6 cut(s)	14, 392, 1033, 2468, 2605, 3278
Ppu21I	YACGTR	1 cut(s)	529
PpuMI	RGGWCCY	1 cut(s)	2915
PshAI	GACNNNNGTC	1 cut(s)	1459
PshBI	ATTAAT	1 cut(s)	2808
PsiI	TTATAA	1 cut(s)	833
Psp124BI	GAGCTC	2 cut(s)	490, 3754
Psp5II	RGGWCCY	1 cut(s)	2915
Psp6I	CCWGG	5 cut(s)	330, 573, 596, 2454, 3467
PspFI	CCCAGC	4 cut(s)	98, 259, 742, 1334
PspGI	CCWGG	5 cut(s)	330, 573, 596, 2454, 3467
PspLI	CGTACG	1 cut(s)	754
PspN4I	GGNNCC	8 cut(s)	40, 90, 503, 584, 1438, 1928, 2639, 3187
PspPI	GGNCC	8 cut(s)	39, 502, 767, 2351, 2915, 3186, 3407, 3441
PspPPI	RGGWCCY	1 cut(s)	2915
PspXI	VCTCGAGB	1 cut(s)	958
PsuI	RGATCY	4 cut(s)	327, 982, 3224, 3481
PvuII	CAGCTG	1 cut(s)	3233
RsaI	GTAC	7 cut(s)	756, 1349, 1882, 2165, 2178, 2796, 3321
RsaNI	GTAC	7 cut(s)	755, 1348, 1881, 2164, 2177, 2795, 3320
RseI	CAYNNNNRTG	3 cut(s)	527, 2509, 2852
SacI	GAGCTC	2 cut(s)	490, 3754
SacII	CCGCGG	1 cut(s)	72
SalI	GTCGAC	1 cut(s)	17
SatI	GCNGC	5 cut(s)	48, 847, 2025, 3663, 3675
Sau96I	GGNCC	8 cut(s)	39, 502, 767, 2351, 2915, 3186, 3407, 3441
ScaI	AGTACT	1 cut(s)	2178
SchI	GAGTC	6 cut(s)	14, 392, 1034, 2468, 2606, 3278
ScrFI	CCNGG	7 cut(s)	332, 575, 598, 770, 2456, 3184, 3469
SduI	GDGCHC	5 cut(s)	490, 540, 2663, 2861, 3754
SfcI	CTRYAG	3 cut(s)	2233, 2790, 3228
Sfr274I	CTCGAG	3 cut(s)	958, 2360, 3286
Sfr303I	CCGCGG	1 cut(s)	72
SfuI	TTCGAA	1 cut(s)	1047
SgrBI	CCGCGG	1 cut(s)	72
SinI	GGWCC	4 cut(s)	39, 2915, 3407, 3441
SlaI	CTCGAG	3 cut(s)	958, 2360, 3286
SmiMI	CAYNNNNRTG	3 cut(s)	527, 2509, 2852
SmlI	CTYRAG	7 cut(s)	958, 1981, 2285, 2360, 2594, 2621, 3286
SmoI	CTYRAG	7 cut(s)	958, 1981, 2285, 2360, 2594, 2621, 3286
SpeI	ACTAGT	1 cut(s)	3116
SphI	GCATGC	1 cut(s)	2514
SsiI	CCGC	8 cut(s)	32, 69, 71, 847, 1019, 1816, 3635, 3674
SspI	AATATT	2 cut(s)	381, 2812
SstI	GAGCTC	2 cut(s)	490, 3754
StyD4I	CCNGG	7 cut(s)	330, 573, 596, 768, 2454, 3182, 3467
StyI	CCWWGG	4 cut(s)	481, 647, 1248, 1361
TaaI	ACNGT	7 cut(s)	130, 729, 2163, 2168, 3220, 3278, 3399
TaiI	ACGT	3 cut(s)	531, 760, 2666
TatI	WGTACW	3 cut(s)	1347, 2163, 2176
TauI	GCSGC	2 cut(s)	849, 3677
TfiI	GAWTC	8 cut(s)	235, 1304, 1501, 1718, 1727, 1850, 3295, 3733
TseFI	GTSAC	9 cut(s)	124, 554, 703, 729, 788, 2441, 2573, 2648, 3281
TseI	GCWGC	3 cut(s)	47, 2024, 3662
Tsp45I	GTSAC	9 cut(s)	124, 554, 703, 729, 788, 2441, 2573, 2648, 3281
TspGWI	ACGGA	2 cut(s)	1310, 2592
Van91I	CCANNNNNTGG	2 cut(s)	2135, 2685
VpaK11BI	GGWCC	4 cut(s)	39, 2915, 3407, 3441
VspI	ATTAAT	1 cut(s)	2808
XapI	RAATTY	6 cut(s)	1042, 1145, 1615, 1873, 1948, 3521
XbaI	TCTAGA	2 cut(s)	1277, 2047
XceI	RCATGY	3 cut(s)	853, 2514, 3026
XhoI	CTCGAG	3 cut(s)	958, 2360, 3286
XmaJI	CCTAGG	1 cut(s)	1361
XmiI	GTMKAC	3 cut(s)	18, 570, 3598
XmnI	GAANNNNTTC	4 cut(s)	342, 2121, 3041, 3110
ZrmI	AGTACT	1 cut(s)	2178
Zsp2I	ATGCAT	3 cut(s)	2371, 2845, 3158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.