Rmu_sc0014112.1_g000004

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014112.1
OG0000546
Physical Location & Seq
Reverse (-)
13670 .. 14467
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014112.1_g000004.1.cds

Sequence Viewer

Length: 678 bp
atgccgatgctcgagaactctgtccccgattcgtcgtcaaggtcaacgtcgtcatttgcgattggagagcccagatgtcatttgggacgcatgaacccacagtggctgccgccgccgccctatgtgttcctcagctccggaggttacgccatgggcaatctctacactgctctggttgaagagggcatacctacaattcgagataatgaaaagctggagttgggatctgggctgagctcagaagagaaggaaaaaatgtttgtgaagacgttgcttccacaactctgcacatcaattgaagaatcgaagcttgtgattgtcatcatttcaagaaattatctgaattcgagctggtgcttggagcaggttgccaaggctgttgaatgcaacaaactaatactccctgttttcgatgatgtggatccaattgaactccgacttcaagagggatcagttgcggaagctttcttcaagcacgaagaaacctttaaagacaacccagacaaggtgcgaaaatggagagaagctataaatcgcgtacccatatcggttggagttgcggcagctttcttcaacaacaaagaagcctttaaagacaacccagataaggcgcgaaaatggagagaagctatatatcaaggacccatatcggtaggatggtctttacagtctaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

225

Amino Acids

25.34

Weight (kDa)

5.6

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AccII CGCG 2 cut(s) 537, 613
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 5 cut(s) 110, 113, 116, 458, 560
AclWI GGATC 4 cut(s) 232, 416, 429, 457
AcsI RAATTY 1 cut(s) 343
AfaI GTAC 1 cut(s) 540
AfiI CCNNNNNNNGG 2 cut(s) 505, 607
AgsI TTSAA 8 cut(s) 179, 299, 330, 383, 431, 443, 472, 574
AluBI AGCT 9 cut(s) 135, 214, 237, 310, 351, 464, 527, 566, 629
AluI AGCT 9 cut(s) 135, 214, 237, 310, 351, 464, 527, 566, 629
Alw21I GWGCWC 1 cut(s) 239
AlwI GGATC 4 cut(s) 232, 416, 429, 457
AlwNI CAGNNNCTG 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 137
ApeKI GCWGC 2 cut(s) 106, 563
ApoI RAATTY 1 cut(s) 343
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 1 cut(s) 641
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 641
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 2 cut(s) 72, 239
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 239
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 93, 575
BccI CCATC 1 cut(s) 651
BfaI CTAG 1 cut(s) 672
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 6 cut(s) 107, 110, 113, 116, 561, 564
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 6 cut(s) 108, 111, 114, 117, 562, 565
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 641
BmiI GGNNCC 2 cut(s) 423, 643
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 233
BsaBI GATNNNNATC 2 cut(s) 320, 420
BsaJI CCNNGG 2 cut(s) 150, 372
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 607
Bse8I GATNNNNATC 2 cut(s) 320, 420
BseAI TCCGGA 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 150, 372
BseGI GGATG 1 cut(s) 662
BseJI GATNNNNATC 2 cut(s) 320, 420
BseLI CCNNNNNNNGG 2 cut(s) 505, 607
BseMII CTCAG 3 cut(s) 145, 224, 252
BseXI GCAGC 2 cut(s) 93, 575
BsgI GTGCAG 1 cut(s) 271
Bsh1236I CGCG 2 cut(s) 537, 613
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 8, 99
BslI CCNNNNNNNGG 2 cut(s) 505, 607
BsmFI GGGAC 2 cut(s) 8, 99
BsmI GAATGC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 72, 239
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 3 cut(s) 224, 421, 449
Bsp1720I GCTNAGC 1 cut(s) 233
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 5 cut(s) 110, 113, 116, 458, 560
BspCNI CTCAG 3 cut(s) 144, 225, 251
BspEI TCCGGA 1 cut(s) 137
BspFNI CGCG 2 cut(s) 537, 613
BspLI GGNNCC 2 cut(s) 423, 643
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 4 cut(s) 232, 416, 429, 457
BssECI CCNNGG 2 cut(s) 150, 372
BssMI GATC 3 cut(s) 224, 421, 449
BssT1I CCWWGG 2 cut(s) 150, 372
Bst4CI ACNGT 2 cut(s) 102, 669
Bst6I CTCTTC 2 cut(s) 174, 237
BstDEI CTNAG 3 cut(s) 131, 233, 238
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 662
BstFNI CGCG 2 cut(s) 537, 613
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 3 cut(s) 227, 424, 452
BstMBI GATC 3 cut(s) 224, 421, 449
BstMWI GCNNNNNNNGC 2 cut(s) 112, 115
BstUI CGCG 2 cut(s) 537, 613
BstV1I GCAGC 2 cut(s) 93, 575
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 2 cut(s) 224, 421
BstYI RGATCY 2 cut(s) 224, 421
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 662
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 2 cut(s) 107, 165
BveI ACCTGC 1 cut(s) 355
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 641
CseI GACGC 1 cut(s) 96
Csp6I GTAC 1 cut(s) 539
CviAII CATG 2 cut(s) 91, 151
CviQI GTAC 1 cut(s) 539
DdeI CTNAG 3 cut(s) 131, 233, 238
DpnI GATC 3 cut(s) 226, 423, 451
DpnII GATC 3 cut(s) 224, 421, 449
DraI TTTAAA 2 cut(s) 490, 592
Eam1104I CTCTTC 2 cut(s) 174, 237
EarI CTCTTC 2 cut(s) 174, 237
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 2 cut(s) 150, 372
Eco24I GRGCYC 2 cut(s) 72, 239
Eco47I GGWCC 1 cut(s) 641
Eco53kI GAGCTC 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 11
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 150, 372
EcoT38I GRGCYC 2 cut(s) 72, 239
ErhI CCWWGG 2 cut(s) 150, 372
FaeI CATG 2 cut(s) 94, 154
FaiI YATR 9 cut(s) 92, 123, 152, 188, 530, 545, 632, 634, 647
FaqI GGGAC 2 cut(s) 8, 99
FatI CATG 2 cut(s) 90, 150
Fnu4HI GCNGC 6 cut(s) 107, 110, 113, 116, 561, 564
FokI GGATG 1 cut(s) 669
FriOI GRGCYC 2 cut(s) 72, 239
Fsp4HI GCNGC 6 cut(s) 107, 110, 113, 116, 561, 564
FspBI CTAG 1 cut(s) 672
GlaI GCGC 1 cut(s) 612
GluI GCNGC 6 cut(s) 107, 110, 113, 116, 561, 564
GsuI CTGGAG 1 cut(s) 236
HapII CCGG 1 cut(s) 138
HgaI GACGC 1 cut(s) 96
HhaI GCGC 1 cut(s) 613
Hin1II CATG 2 cut(s) 94, 154
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HindIII AAGCTT 2 cut(s) 308, 462
HinfI GANTC 2 cut(s) 29, 302
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 3 cut(s) 241, 342, 437
Hpy188III TCNNGA 5 cut(s) 13, 138, 200, 330, 443
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 2 cut(s) 37, 52
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 2 cut(s) 102, 669
HpyCH4IV ACGT 2 cut(s) 47, 269
HpyCH4V TGCA 2 cut(s) 288, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 115
HpyF3I CTNAG 3 cut(s) 131, 233, 238
HpySE526I ACGT 2 cut(s) 47, 269
Hsp92II CATG 2 cut(s) 94, 154
HspAI GCGC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 3 cut(s) 224, 421, 449
LmnI GCTCC 2 cut(s) 140, 361
Lsp1109I GCAGC 2 cut(s) 93, 575
MaeI CTAG 1 cut(s) 672
MaeII ACGT 2 cut(s) 47, 269
MaeIII GTNAC 1 cut(s) 143
MalI GATC 3 cut(s) 226, 423, 451
MboI GATC 3 cut(s) 224, 421, 449
MboII GAAGA 7 cut(s) 191, 254, 277, 311, 460, 491, 562
MfeI CAATTG 2 cut(s) 294, 426
MflI RGATCY 2 cut(s) 224, 421
MhlI GDGCHC 2 cut(s) 72, 239
MluCI AATT 5 cut(s) 195, 294, 334, 343, 426
MmeI TCCRAC 2 cut(s) 460, 532
MnlI CCTC 4 cut(s) 134, 140, 175, 439
MroI TCCGGA 1 cut(s) 137
MseI TTAA 2 cut(s) 489, 591
MspI CCGG 1 cut(s) 138
MunI CAATTG 2 cut(s) 294, 426
Mva1269I GAATGC 1 cut(s) 389
MvnI CGCG 2 cut(s) 537, 613
MwoI GCNNNNNNNGC 2 cut(s) 112, 115
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 224, 421, 449
NlaIII CATG 2 cut(s) 94, 154
NlaIV GGNNCC 2 cut(s) 423, 643
PaeR7I CTCGAG 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 2 cut(s) 29, 302
PkrI GCNGC 6 cut(s) 108, 111, 114, 117, 562, 565
PpuMI RGGWCCY 1 cut(s) 641
Psp124BI GAGCTC 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 641
PspN4I GGNNCC 2 cut(s) 423, 643
PspPI GGNCC 1 cut(s) 641
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 2 cut(s) 224, 421
RsaI GTAC 1 cut(s) 540
RsaNI GTAC 1 cut(s) 539
SacI GAGCTC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 489, 591
SatI GCNGC 6 cut(s) 107, 110, 113, 116, 561, 564
Sau3AI GATC 3 cut(s) 224, 421, 449
Sau96I GGNCC 1 cut(s) 641
SduI GDGCHC 2 cut(s) 72, 239
Sfr274I CTCGAG 1 cut(s) 11
SinI GGWCC 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 5 cut(s) 195, 294, 334, 343, 426
SsiI CCGC 5 cut(s) 110, 113, 116, 458, 560
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 239
StyI CCWWGG 2 cut(s) 150, 372
TaaI ACNGT 2 cut(s) 102, 669
TaiI ACGT 2 cut(s) 50, 272
TaqI TCGA 5 cut(s) 12, 199, 305, 347, 411
TasI AATT 5 cut(s) 195, 294, 334, 343, 426
TauI GCSGC 4 cut(s) 112, 115, 118, 563
TfiI GAWTC 2 cut(s) 29, 302
Tru1I TTAA 2 cut(s) 489, 591
Tru9I TTAA 2 cut(s) 489, 591
TscAI CASTG 2 cut(s) 107, 172
TseI GCWGC 2 cut(s) 106, 563
TspDTI ATGAA 2 cut(s) 107, 222
TspRI CASTG 2 cut(s) 107, 172
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 11
XspI CTAG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.