Rw7G036940

ADP binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000546
Physical Location & Seq
Forward (+)
56014657 .. 56019730
5074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036940.1

Sequence Viewer

Length: 3282 bp
ATGGTTTCAGCATCATTTAACTGTTGCCAGAATCGAACTGGCATATGGGGGCAAATTAGCCCTCTTAAGCTTAACTTGTCCGACCGAACGGACTTCCAAGTCGAAGGCGAGTTCGAACTAATATGCAGGAATTTAGGCTTCTCGGGCCCAGACGAGTTTGCTATCCCCGCGGCCGCCTGGGAGACCCAGTCGACGAACTCGGATCCTCTCCCCCCCTACCGGATGGAATGGCCCGAGAGTGCGAAACCCGAGGAAGTGAAGCACCCAAAGGAGGTCGAGGAAGAGGAGAAATGGGAGATGGGTCTGCAGTCACGTCGACTGCTTAAGCCGCCGCCGCCGATGAGTGTTCCGGTGATTGACAATGCATGCTCGACATGGGACATTGTGTGGGGCTTAGGGCCCCAACGTGAAACTTCTGATGAAGAAGTAGAAGAAGAGGATAATTTGATTACTTCTGATGAATTTTTTTATTTTTGGTCTCATACTTCTGATGATGAAGATGAAGAAGATCGTATTTCGATTAAAGAGATTGTATCAAGCTCCGGGTATTCTTTTACTACTTGGAATGACGATGACTCTTCCAGCACTACCACAGACTCCTCCACCATTTCTTCTTGGGAAAAGGAAACCTCTCGCGGGTATTTACCCCTGTATGAAGGAATTTCCGATAAGGTAACTAAAGCGATGGATCCCAGCTGGAAGACCCGACAGAAGCCAAAGTTGGTTAGGCGCAATGCGGTGAAGAACATTGACTATGATGACGTTACATCGCCTTCCTCGCTCTGCCACCACTCCGCAGGGTCACCACTCGACTCAGGCTCGGTCAATATTTCAGACCAGACGAGCTTCCAAGTAGAAGGAAACGACAGTGAGTTTGAACAGATATGCATGAATTTGGGATTCTCAGGCCCTGAGTCCTTGGAGTTCCGCTCCACAAACTCGGAATTGAAGCTAAACGAGAACTGCAATCGCAAAGAAATGGAAGAATTAGAAATTGATAAAAGAGCAGATGAAGAAACTAATTTGAGAGCAGAAGAATTTATAAACTTCAAACCAGGAGTATTACATAATAGCGCTTGTTCTTTTATGGATCCAAAACTGTTGGCAATTGAGCCCGACTCTTTCATTCCATTACAAACTTCTGATGTCTCTCTGCACTTGATGGGACAACTTCCGTGTAATTTTTTGTTGAACTTCAAGTTCTTGACTGCATTGCATCTTAGTCGAAAGCTGGATTTTGATTTGCCAAGTTCAATTGGGGATGTAAAATCCTTACGATGTCTTGATGTCTCATATACACCAATCTCATGGTTGCCCGAATCAATAGGTTTTCTTCACAAGTTACAGACCCTAAAACTCAGAAGTTGTAGAAACCTCTTTCAGTTGCCTGATTCAATTACTTCTCTTCATGAGTTACAGACCTTACATCTCAGAGGTTGTGTAAACCTTGTTGCTTTACCTAAGGGAATAAAGGTACTAATAAAGCTGCATGATCTTGATGTTATGAACTGTCCGAACTTGAAACTTTCGGAGGAGCCAAATGAGAAGCTGATTTCTTCAGAAATAAATTTGTCAGAGTCATTGGAAGAAAAGGTTATGCATGAAGATGAAGAATATGACAGTTTACCCAAACAATTAGAAAGGTTATTTCTGTATTGTTCTCTCTTTCCATCACATTATGAGTTTAGAAGAGATGAGTTGGTTCAAATGTGGATAGCTGAAGGCCTTATTGAAGAGATCCACGGAGGGGTAATGGAGGATATTGGTGGTGAGTATTTCATGTCTTTGCAGAACAAAGGTTTTCTTGTGCCTTCAAGATGTGACTACAGAGTGGATTTTGATTCGGTATTTTCTGTGCCCATTTATAATCCTGGCCACTTATTGTATAAAGGAAATCCAGTCAAGTTTTCACGTTTGGGAAGAACAATTGCTCCGGTTGAGTATTTCGTAGCTGTGGATGGTAAGTTGGATGGAGCCTTGGAAATGACACAGCATTTGTCTTTAATTTGCGAGGACATTGATGAGATGACTTTTGGGGTTCTTAAAAAATTTAAGCATTTGCGTACACTTCTTGTGCTTGCTGGTTGTGGATCTTCCATCAAATATGTTCCTCGTGATCTGTTTTTGAGCTTAAAGGAACTGAGAACACTGAATTTGAGTCAAACACTCGTCTCTGAATTGCCAAGTTCCATTCGAAACGCAAAGTCATTACGATACCTTGATCTCTCCAATACTCTTATTACACGGCTGCCGGAATCGATAGATTCTCTTCACACTTTACAGACTGTAAGACTCAGAGGTTGTGTACACTTTCTTGAATTACCAAAGGGGATGAAGAAGCTAGTCAATCTACGACATATCGATTTGGATATCATTCGACAGTTAAGGTCCATGCCTCCCTACTTGGGAAGCTTAACCAACCTTCAGACTTTGTCAGCGTTTCTTGTTGGTCGAGATGCAGGGTGCCGTATAGGAGAGCTTAAGAATTTGAATGATCTCAGAGGAGTACTTCGCATTTCAAGGCTTGAAAACGTGTCATGCCAGGAAGAGGCTAAGGCAGCTGCGTTGATCGAAAAGATATACCTGCAAAGGTTGGAGCTCCGCTGGAGTAATGTGTTCGCTGAGAACACAGAAGAGCAAGAGAAAATTCTTGAATGTCTTCAACCCCATGCTGGCCTTAAAGAGCTACAAATACAACAATATGCCGGGTCAAGACTTCCAACTTGGATTAGCAATCCATGTTTTGCTGAACTTATTGTTCTTACTCTCTACAGATGCAAAAACTGTGAGCTTCTCCCATCTATTGGGCAGTTACCAATGCTAAAGTCTCTTTCTATTATAGAAATGAATGAGGTGAAAGAAATCAACTACCAGTTTTTGGGAAAGAAAAACCCAGTTGATCAAGGGTTTCATGCATTTTTGAAACTGGAGATACTAGAAGTTGACATCATGCTCAATTTGAAACTGTGGAAGGATGTAAACATTGGTGACTTACCTTCCTTACTTAAACTCACGGTTGATTCTTGCCCTGAACTTACCACTCTCCCATCACTCTCTTGGCTCAAATCCCTCAAGCATTTGGAACTCAGGCGCTGTCCAAAAATTCTGAACTGGCATTGTGATGGACTACCAGCATCACTTGAGTCTTTCATAGTAATAGATTGCCTGGAACTGAAAGAATGGTGCCGCATGATGGAAGGTGAAGATTGGAGGAAGATATGCCATCTCCCTAGCATATGGCTTGATTGTGAAGAGGTATCCAAGTCCCAGAATATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04016 ko04075 ko04931 map04014 map04016 map04075 map04931
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1093

Amino Acids

124.7

Weight (kDa)

5.03

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 396 - 504 8.6e-14 Leucine-rich repeat region
WHD_DRP PF23559 555 - 603 2.5e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 731 - 966 1.5e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 824 - 949 3.5e-39 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 910 - 1028 4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1043, 1866
AasI GACNNNNNNGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 2592
AccB1I GGYRCC 2 cut(s) 2463, 3183
AccB7I CCANNNNNTGG 3 cut(s) 2804, 2878, 3274
AccBSI CCGCTC 1 cut(s) 930
AccI GTMKAC 2 cut(s) 191, 316
AccII CGCG 2 cut(s) 170, 636
AclWI GGATC 8 cut(s) 197, 210, 683, 696, 1085, 1098, 1732, 2098
AcoI YGGCCR 2 cut(s) 171, 1873
AcuI CTGAAG 3 cut(s) 1542, 1740, 2408
AfaI GTAC 4 cut(s) 1476, 2065, 2307, 2508
AfeI AGCGCT 1 cut(s) 1075
AflII CTTAAG 3 cut(s) 65, 323, 2480
AflIII ACRYGT 1 cut(s) 2532
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 5 cut(s) 176, 1054, 1870, 2541, 3165
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AloI GAACNNNNNNTCC 2 cut(s) 1915, 1947
Alw21I GWGCWC 1 cut(s) 2601
Alw26I GTCTC 6 cut(s) 176, 483, 1153, 1294, 2176, 2832
AlwI GGATC 8 cut(s) 197, 210, 683, 696, 1085, 1098, 1732, 2098
AlwNI CAGNNNCTG 2 cut(s) 911, 3093
Ama87I CYCGRG 3 cut(s) 142, 233, 248
Aor51HI AGCGCT 1 cut(s) 1075
AoxI GGCC 8 cut(s) 145, 171, 230, 398, 907, 1723, 1873, 2674
ApaI GGGCCC 2 cut(s) 149, 402
ApeKI GCWGC 4 cut(s) 1486, 2248, 2558, 2561
ArsI GACNNNNNNTTYG 4 cut(s) 857, 889, 1978, 2010
Asp700I GAANNNNTTC 1 cut(s) 2658
AspLEI GCGC 3 cut(s) 732, 1076, 3093
AspS9I GGNCC 7 cut(s) 145, 146, 231, 398, 399, 908, 2388
AsuC2I CCSGG 2 cut(s) 544, 2707
AsuHPI GGTGA 7 cut(s) 364, 751, 795, 1781, 2866, 2999, 3212
AsuII TTCGAA 2 cut(s) 114, 2194
AvaI CYCGRG 3 cut(s) 142, 233, 248
AvaII GGWCC 1 cut(s) 2388
AxyI CCTNAGG 1 cut(s) 1461
BaeGI GKGCMC 3 cut(s) 149, 402, 1860
BalI TGGCCA 1 cut(s) 1875
BamHI GGATCC 3 cut(s) 202, 688, 1090
BanI GGYRCC 2 cut(s) 2463, 3183
BanII GRGCYC 4 cut(s) 149, 402, 1116, 2601
BauI CACGAG 1 cut(s) 2112
BbsI GAAGAC 2 cut(s) 707, 2651
Bbv12I GWGCWC 1 cut(s) 2601
BbvI GCAGC 4 cut(s) 1473, 2235, 2548, 2570
BceAI ACGGC 2 cut(s) 2261, 2451
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 1205, 1239
BciT130I CCWGG 5 cut(s) 178, 1056, 1872, 2543, 3167
BciVI GTATCC 1 cut(s) 3268
BclI TGATCA 1 cut(s) 2899
BcnI CCSGG 2 cut(s) 544, 2707
BcoDI GTCTC 6 cut(s) 176, 483, 1153, 1294, 2176, 2832
BfaI CTAG 3 cut(s) 2342, 2936, 3231
BfmI CTRYAG 3 cut(s) 305, 1825, 2770
BfoI RGCGCY 2 cut(s) 1077, 3094
BfrI CTTAAG 3 cut(s) 65, 323, 2480
BfuAI ACCTGC 1 cut(s) 2592
BfuI GTATCC 1 cut(s) 3268
BmcAI AGTACT 1 cut(s) 2508
Bme1390I CCNGG 7 cut(s) 178, 544, 1056, 1872, 2543, 2707, 3167
Bme18I GGWCC 1 cut(s) 2388
BmeT110I CYCGRG 3 cut(s) 142, 233, 248
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 7 cut(s) 145, 146, 231, 398, 399, 908, 2388
BmrFI CCNGG 7 cut(s) 178, 544, 1056, 1872, 2543, 2707, 3167
BmrI ACTGGG 2 cut(s) 181, 2888
BmsI GCATC 5 cut(s) 20, 1225, 2446, 2765, 3143
BmuI ACTGGG 2 cut(s) 181, 2888
BpiI GAAGAC 2 cut(s) 707, 2651
BplI GAGNNNNNCTC 4 cut(s) 914, 946, 1103, 1135
BpmI CTGGAG 2 cut(s) 2626, 2948
Bpu10I CCTNAGC 2 cut(s) 394, 2553
Bpu14I TTCGAA 2 cut(s) 114, 2194
BpuEI CTTGAG 2 cut(s) 3056, 3161
BpuMI CCSGG 2 cut(s) 544, 2707
Bsa29I ATCGAT 2 cut(s) 2258, 2361
BsaI GGTCTC 2 cut(s) 176, 483
BsaJI CCNNGG 6 cut(s) 168, 177, 249, 918, 1741, 1977
BsaWI WCCGGW 3 cut(s) 219, 349, 1933
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 1915, 1945
Bse1I ACTGG 7 cut(s) 43, 187, 1898, 2872, 2894, 2931, 3116
Bse21I CCTNAGG 1 cut(s) 1461
Bse3DI GCAATG 2 cut(s) 739, 1211
BseBI CCWGG 5 cut(s) 178, 1056, 1872, 2543, 3167
BseCI ATCGAT 2 cut(s) 2258, 2361
BseDI CCNNGG 6 cut(s) 168, 177, 249, 918, 1741, 1977
BseGI GGATG 6 cut(s) 228, 1267, 1963, 1975, 2337, 2980
BseMI GCAATG 2 cut(s) 739, 1211
BseNI ACTGG 7 cut(s) 43, 187, 1898, 2872, 2894, 2931, 3116
BseRI GAGGAG 4 cut(s) 299, 589, 1547, 2517
BseSI GKGCMC 3 cut(s) 149, 402, 1860
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 4 cut(s) 1473, 2235, 2548, 2570
BseYI CCCAGC 1 cut(s) 692
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 2 cut(s) 170, 636
Bsh1285I CGRYCG 2 cut(s) 85, 174
BshFI GGCC 8 cut(s) 147, 173, 232, 400, 909, 1725, 1875, 2676
BshNI GGYRCC 2 cut(s) 2463, 3183
BshVI ATCGAT 2 cut(s) 2258, 2361
BsiEI CGRYCG 2 cut(s) 85, 174
BsiHKAI GWGCWC 1 cut(s) 2601
BsiHKCI CYCGRG 3 cut(s) 142, 233, 248
BsiSI CCGG 6 cut(s) 220, 350, 543, 1934, 2252, 2706
BslFI GGGAC 3 cut(s) 392, 1179, 3250
BsmAI GTCTC 6 cut(s) 176, 483, 1153, 1294, 2176, 2832
BsmBI CGTCTC 1 cut(s) 2176
BsmFI GGGAC 3 cut(s) 392, 1179, 3250
BsnI GGCC 8 cut(s) 147, 173, 232, 400, 909, 1725, 1875, 2676
Bso31I GGTCTC 2 cut(s) 176, 483
BsoBI CYCGRG 3 cut(s) 142, 233, 248
Bsp119I TTCGAA 2 cut(s) 114, 2194
Bsp120I GGGCCC 2 cut(s) 145, 398
Bsp1286I GDGCHC 5 cut(s) 149, 402, 1116, 1860, 2601
Bsp1407I TGTACA 1 cut(s) 2305
BspANI GGCC 8 cut(s) 147, 173, 232, 400, 909, 1725, 1875, 2676
BspDI ATCGAT 2 cut(s) 2258, 2361
BspFNI CGCG 2 cut(s) 170, 636
BspHI TCATGA 1 cut(s) 1408
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 1 cut(s) 2592
BspPI GGATC 8 cut(s) 197, 210, 683, 696, 1085, 1098, 1732, 2098
BspQI GCTCTTC 1 cut(s) 2628
BspT104I TTCGAA 2 cut(s) 114, 2194
BspT107I GGYRCC 2 cut(s) 2463, 3183
BspTI CTTAAG 3 cut(s) 65, 323, 2480
BspTNI GGTCTC 2 cut(s) 176, 483
BsrBI CCGCTC 1 cut(s) 930
BsrDI GCAATG 2 cut(s) 739, 1211
BsrGI TGTACA 1 cut(s) 2305
BsrI ACTGG 7 cut(s) 43, 187, 1898, 2872, 2894, 2931, 3116
BssECI CCNNGG 6 cut(s) 168, 177, 249, 918, 1741, 1977
BssSI CACGAG 1 cut(s) 2112
BssT1I CCWWGG 2 cut(s) 918, 1977
Bst2BI CACGAG 1 cut(s) 2112
Bst2UI CCWGG 5 cut(s) 178, 1056, 1872, 2543, 3167
BstAFI CTTAAG 3 cut(s) 65, 323, 2480
BstAUI TGTACA 1 cut(s) 2305
BstBI TTCGAA 2 cut(s) 114, 2194
BstC8I GCNNGC 3 cut(s) 367, 2079, 2674
BstDSI CCRYGG 2 cut(s) 168, 1741
BstEII GGTNACC 1 cut(s) 801
BstF5I GGATG 6 cut(s) 228, 1267, 1963, 1975, 2337, 2980
BstFNI CGCG 2 cut(s) 170, 636
BstH2I RGCGCY 2 cut(s) 1077, 3094
BstHHI GCGC 3 cut(s) 732, 1076, 3093
BstMAI GTCTC 6 cut(s) 176, 483, 1153, 1294, 2176, 2832
BstMCI CGRYCG 2 cut(s) 85, 174
BstMWI GCNNNNNNNGC 6 cut(s) 144, 167, 328, 334, 778, 2558
BstNI CCWGG 5 cut(s) 178, 1056, 1872, 2543, 3167
BstNSI RCATGY 1 cut(s) 369
BstPI GGTNACC 1 cut(s) 801
BstSCI CCNGG 7 cut(s) 176, 542, 1054, 1870, 2541, 2705, 3165
BstSFI CTRYAG 3 cut(s) 305, 1825, 2770
BstSLI GKGCMC 3 cut(s) 149, 402, 1860
BstUI CGCG 2 cut(s) 170, 636
BstV1I GCAGC 4 cut(s) 1473, 2235, 2548, 2570
BstV2I GAAGAC 2 cut(s) 707, 2651
BstX2I RGATCY 5 cut(s) 202, 688, 1090, 1737, 2090
BstXI CCANNNNNNTGG 1 cut(s) 1308
BstYI RGATCY 5 cut(s) 202, 688, 1090, 1737, 2090
BstZI CGGCCG 1 cut(s) 171
Bsu15I ATCGAT 2 cut(s) 2258, 2361
Bsu36I CCTNAGG 1 cut(s) 1461
BsuI GTATCC 1 cut(s) 3268
BsuRI GGCC 8 cut(s) 147, 173, 232, 400, 909, 1725, 1875, 2676
BsuTUI ATCGAT 2 cut(s) 2258, 2361
BtgI CCRYGG 2 cut(s) 168, 1741
BtgZI GCGATG 2 cut(s) 698, 753
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 6 cut(s) 228, 1267, 1963, 1975, 2337, 2980
BtsIMutI CAGTG 2 cut(s) 874, 2147
BveI ACCTGC 1 cut(s) 2592
Cac8I GCNNGC 3 cut(s) 367, 2079, 2674
CaiI CAGNNNCTG 2 cut(s) 911, 3093
CciI TCATGA 1 cut(s) 1408
CciNI GCGGCCGC 1 cut(s) 171
CfoI GCGC 3 cut(s) 732, 1076, 3093
Cfr13I GGNCC 7 cut(s) 145, 146, 231, 398, 399, 908, 2388
Cfr42I CCGCGG 1 cut(s) 171
ClaI ATCGAT 2 cut(s) 2258, 2361
Csp6I GTAC 4 cut(s) 1475, 2064, 2306, 2507
CviQI GTAC 4 cut(s) 1475, 2064, 2306, 2507
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 2 cut(s) 171, 1873
EagI CGGCCG 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 2599
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 918, 1977
Eco147I AGGCCT 1 cut(s) 1725
Eco24I GRGCYC 4 cut(s) 149, 402, 1116, 2601
Eco31I GGTCTC 2 cut(s) 176, 483
Eco32I GATATC 1 cut(s) 2371
Eco47I GGWCC 1 cut(s) 2388
Eco47III AGCGCT 1 cut(s) 1075
Eco52I CGGCCG 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 2599
Eco57I CTGAAG 3 cut(s) 1542, 1740, 2408
Eco81I CCTNAGG 1 cut(s) 1461
Eco88I CYCGRG 3 cut(s) 142, 233, 248
Eco91I GGTNACC 1 cut(s) 801
EcoICRI GAGCTC 1 cut(s) 2599
EcoO109I RGGNCCY 3 cut(s) 398, 399, 908
EcoO65I GGTNACC 1 cut(s) 801
EcoRII CCWGG 5 cut(s) 176, 1054, 1870, 2541, 3165
EcoRV GATATC 1 cut(s) 2371
EcoT14I CCWWGG 2 cut(s) 918, 1977
EcoT22I ATGCAT 4 cut(s) 367, 890, 1602, 2917
EcoT38I GRGCYC 4 cut(s) 149, 402, 1116, 2601
ErhI CCWWGG 2 cut(s) 918, 1977
Esp3I CGTCTC 1 cut(s) 2176
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 1788, 1820
FaqI GGGAC 3 cut(s) 392, 1179, 3250
FauI CCCGC 2 cut(s) 175, 629
FauNDI CATATG 2 cut(s) 44, 3236
FbaI TGATCA 1 cut(s) 2899
FblI GTMKAC 2 cut(s) 191, 316
FokI GGATG 6 cut(s) 235, 1274, 1970, 1982, 2344, 2987
FriOI GRGCYC 4 cut(s) 149, 402, 1116, 2601
FspBI CTAG 3 cut(s) 2342, 2936, 3231
GlaI GCGC 3 cut(s) 731, 1075, 3092
GsaI CCCAGC 1 cut(s) 696
GsuI CTGGAG 2 cut(s) 2626, 2948
HaeII RGCGCY 2 cut(s) 1077, 3094
HaeIII GGCC 8 cut(s) 147, 173, 232, 400, 909, 1725, 1875, 2676
HapII CCGG 6 cut(s) 220, 350, 543, 1934, 2252, 2706
HhaI GCGC 3 cut(s) 732, 1076, 3093
Hin6I GCGC 3 cut(s) 730, 1074, 3091
HinP1I GCGC 3 cut(s) 730, 1074, 3091
HincII GTYRAC 3 cut(s) 192, 317, 2944
HindII GTYRAC 3 cut(s) 192, 317, 2944
HindIII AAGCTT 2 cut(s) 68, 2410
HpaII CCGG 6 cut(s) 220, 350, 543, 1934, 2252, 2706
HphI GGTGA 7 cut(s) 364, 751, 795, 1781, 2866, 2999, 3212
Hpy166II GTNNAC 9 cut(s) 192, 317, 1444, 1625, 2066, 2306, 2308, 2944, 2980
Hpy8I GTNNAC 9 cut(s) 192, 317, 1444, 1625, 2066, 2306, 2308, 2944, 2980
Hpy99I CGWCG 2 cut(s) 196, 318
HpyCH4IV ACGT 5 cut(s) 313, 406, 762, 1912, 2532
HpyF10VI GCNNNNNNNGC 6 cut(s) 144, 167, 328, 334, 778, 2558
HpySE526I ACGT 5 cut(s) 313, 406, 762, 1912, 2532
HspAI GCGC 3 cut(s) 730, 1074, 3091
Ksp22I TGATCA 1 cut(s) 2899
KspI CCGCGG 1 cut(s) 171
LguI GCTCTTC 1 cut(s) 2628
LmnI GCTCC 7 cut(s) 545, 935, 1534, 1936, 1973, 2596, 2604
Lsp1109I GCAGC 4 cut(s) 1473, 2235, 2548, 2570
LweI GCATC 5 cut(s) 20, 1225, 2446, 2765, 3143
MaeI CTAG 3 cut(s) 2342, 2936, 3231
MaeII ACGT 5 cut(s) 313, 406, 762, 1912, 2532
MaeIII GTNAC 9 cut(s) 309, 673, 763, 801, 1341, 1413, 1820, 2811, 2987
MbiI CCGCTC 1 cut(s) 930
MfeI CAATTG 3 cut(s) 1107, 1254, 1926
MflI RGATCY 5 cut(s) 202, 688, 1090, 1737, 2090
MhlI GDGCHC 5 cut(s) 149, 402, 1116, 1860, 2601
MlsI TGGCCA 1 cut(s) 1875
MluNI TGGCCA 1 cut(s) 1875
MlyI GAGTC 9 cut(s) 569, 590, 806, 923, 1112, 1586, 2167, 2286, 3152
MmeI TCCRAC 4 cut(s) 105, 1947, 2574, 2744
Mox20I TGGCCA 1 cut(s) 1875
Mph1103I ATGCAT 4 cut(s) 367, 890, 1602, 2917
MroXI GAANNNNTTC 1 cut(s) 2658
MscI TGGCCA 1 cut(s) 1875
MslI CAYNNNNRTG 2 cut(s) 1605, 3120
Msp20I TGGCCA 1 cut(s) 1875
MspA1I CMGCKG 4 cut(s) 170, 696, 2561, 2604
MspCI CTTAAG 3 cut(s) 65, 323, 2480
MspI CCGG 6 cut(s) 220, 350, 543, 1934, 2252, 2706
MspR9I CCNGG 7 cut(s) 178, 544, 1056, 1872, 2543, 2707, 3167
MunI CAATTG 3 cut(s) 1107, 1254, 1926
MvaI CCWGG 5 cut(s) 178, 1056, 1872, 2543, 3167
MvnI CGCG 2 cut(s) 170, 636
MwoI GCNNNNNNNGC 6 cut(s) 144, 167, 328, 334, 778, 2558
NciI CCSGG 2 cut(s) 544, 2707
NdeI CATATG 2 cut(s) 44, 3236
NmuCI GTSAC 4 cut(s) 309, 801, 1820, 2987
NotI GCGGCCGC 1 cut(s) 171
NsiI ATGCAT 4 cut(s) 367, 890, 1602, 2917
NspI RCATGY 1 cut(s) 369
NspV TTCGAA 2 cut(s) 114, 2194
PaeI GCATGC 1 cut(s) 369
PagI TCATGA 1 cut(s) 1408
PceI AGGCCT 1 cut(s) 1725
PciSI GCTCTTC 1 cut(s) 2628
PdmI GAANNNNTTC 1 cut(s) 2658
PfeI GAWTC 8 cut(s) 31, 900, 1319, 1391, 1841, 2255, 2264, 3020
PflFI GACNNNGTC 2 cut(s) 187, 2431
PflMI CCANNNNNTGG 3 cut(s) 2804, 2878, 3274
PleI GAGTC 9 cut(s) 569, 590, 806, 922, 1112, 1585, 2166, 2286, 3151
PpsI GAGTC 9 cut(s) 569, 590, 806, 922, 1112, 1585, 2166, 2286, 3151
PsiI TTATAA 2 cut(s) 1043, 1866
Psp124BI GAGCTC 1 cut(s) 2601
Psp6I CCWGG 5 cut(s) 176, 1054, 1870, 2541, 3165
PspEI GGTNACC 1 cut(s) 801
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 5 cut(s) 176, 1054, 1870, 2541, 3165
PspOMI GGGCCC 2 cut(s) 145, 398
PspPI GGNCC 7 cut(s) 145, 146, 231, 398, 399, 908, 2388
PstI CTGCAG 1 cut(s) 309
PstNI CAGNNNCTG 2 cut(s) 911, 3093
PsuI RGATCY 5 cut(s) 202, 688, 1090, 1737, 2090
PsyI GACNNNGTC 2 cut(s) 187, 2431
PvuII CAGCTG 2 cut(s) 696, 2561
RsaI GTAC 4 cut(s) 1476, 2065, 2307, 2508
RsaNI GTAC 4 cut(s) 1475, 2064, 2306, 2507
RseI CAYNNNNRTG 2 cut(s) 1605, 3120
SacI GAGCTC 1 cut(s) 2601
SacII CCGCGG 1 cut(s) 171
SalI GTCGAC 2 cut(s) 190, 315
SapI GCTCTTC 1 cut(s) 2628
Sau96I GGNCC 7 cut(s) 145, 146, 231, 398, 399, 908, 2388
ScaI AGTACT 1 cut(s) 2508
SchI GAGTC 9 cut(s) 569, 590, 806, 923, 1112, 1586, 2167, 2286, 3152
ScrFI CCNGG 7 cut(s) 178, 544, 1056, 1872, 2543, 2707, 3167
SduI GDGCHC 5 cut(s) 149, 402, 1116, 1860, 2601
SfaNI GCATC 5 cut(s) 20, 1225, 2446, 2765, 3143
SfcI CTRYAG 3 cut(s) 305, 1825, 2770
Sfr303I CCGCGG 1 cut(s) 171
SfuI TTCGAA 2 cut(s) 114, 2194
SgrBI CCGCGG 1 cut(s) 171
SinI GGWCC 1 cut(s) 2388
SmiMI CAYNNNNRTG 2 cut(s) 1605, 3120
SmlI CTYRAG 5 cut(s) 65, 323, 2480, 3071, 3140
SmoI CTYRAG 5 cut(s) 65, 323, 2480, 3071, 3140
SphI GCATGC 1 cut(s) 369
SseBI AGGCCT 1 cut(s) 1725
SspI AATATT 1 cut(s) 829
SspMI CTAG 3 cut(s) 2342, 2936, 3231
SstI GAGCTC 1 cut(s) 2601
StuI AGGCCT 1 cut(s) 1725
StyD4I CCNGG 7 cut(s) 176, 542, 1054, 1870, 2541, 2705, 3165
StyI CCWWGG 2 cut(s) 918, 1977
TaiI ACGT 5 cut(s) 316, 409, 765, 1915, 2535
TaqII GACCGA 2 cut(s) 99, 811
TatI WGTACW 2 cut(s) 2305, 2506
TauI GCSGC 6 cut(s) 173, 176, 331, 334, 337, 3189
TfiI GAWTC 8 cut(s) 31, 900, 1319, 1391, 1841, 2255, 2264, 3020
TscAI CASTG 2 cut(s) 874, 2154
TseFI GTSAC 4 cut(s) 309, 801, 1820, 2987
TseI GCWGC 4 cut(s) 1486, 2248, 2558, 2561
Tsp45I GTSAC 4 cut(s) 309, 801, 1820, 2987
TspGWI ACGGA 3 cut(s) 104, 1164, 1758
TspRI CASTG 2 cut(s) 874, 2154
Tth111I GACNNNGTC 2 cut(s) 187, 2431
Van91I CCANNNNNTGG 3 cut(s) 2804, 2878, 3274
Vha464I CTTAAG 3 cut(s) 65, 323, 2480
VpaK11BI GGWCC 1 cut(s) 2388
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 3054
XmiI GTMKAC 2 cut(s) 191, 316
XmnI GAANNNNTTC 1 cut(s) 2658
XspI CTAG 3 cut(s) 2342, 2936, 3231
ZrmI AGTACT 1 cut(s) 2508
Zsp2I ATGCAT 4 cut(s) 367, 890, 1602, 2917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.