Rmu_sc0011027.1_g000010

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011027.1
OG0000546
Physical Location & Seq
Reverse (-)
25504 .. 27441
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011027.1_g000010.1.cds

Sequence Viewer

Length: 1050 bp
atgaaccgaccaggtgcggcgtcgccgccagcaacatccgatgaagaggtccaacttcaagatccacccccatctcctctcaaactcggcaaagaatcagggaaggagaagaagaaaccagcattagaggccttcaaaacggatgatacctctgaatcagagctgaagacagccgtaaattttttactgattaattatctgtaccatgcgttggttgagaaaggaatatcgacgtttcgagatgatgatgaacttgagaaggctccgcacatcatgaaagcaattgaagaatcaaaattcagcatcgtcatcctttcaaccaactatgctaggccaactcggataatcccagttttctatgatgtggaaccaattgaggtacgacatcaaaccggcaattttggggaagctttctcgaagcataaaaatgccttcaaaggtaacacagaaacggtgcaaaagtggacggacgcacttatcaggttggctaatatgtcgagctgggatctacgacaaggagatccagtcagtgttatttcagaagttgttcaaacagtttcttctgaattaaatttaactaccttacgcgtatccaagggcttagtggcaatggattcaggcgttgaacaagtgccttcatacttggacgtgagcaggtctcctgatgtccgtatgatagggatttctggcatggggggtggcatcggaaaagaagcagagtgggtaccttcagggttgcactctcccattgacagggtacacgaagagaacgagatagagactagaaatgtgaaagaaaatgttgtaattgaggaagacaagagttcagctgaacatttacacggattatttctatattgttccctttttcaggaagattttgaatttagaaaacatcagttggttcagttatggatagctgaaggcctggatatgaaagagagtgagcaagagacaatggaagatgctggcattaagcatttcaatacattggagagtcaaggctattttatgcgctccagatgcgatttcagagtgagttttgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

349

Amino Acids

39.28

Weight (kDa)

5.03

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 645
Acc65I GGTACC 1 cut(s) 724
AccB1I GGYRCC 1 cut(s) 724
AccB7I CCANNNNNTGG 1 cut(s) 211
AccII CGCG 1 cut(s) 588
AciI CCGC 3 cut(s) 17, 26, 266
AclWI GGATC 3 cut(s) 56, 513, 515
AcsI RAATTY 4 cut(s) 178, 296, 571, 884
AcuI CTGAAG 3 cut(s) 185, 714, 942
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 4 cut(s) 203, 381, 726, 759
AfiI CCNNNNNNNGG 3 cut(s) 211, 753, 871
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 9 cut(s) 59, 136, 287, 318, 436, 551, 626, 884, 985
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 2 cut(s) 10, 927
AluBI AGCT 5 cut(s) 163, 410, 501, 830, 920
AluI AGCT 5 cut(s) 163, 410, 501, 830, 920
Alw26I GTCTC 3 cut(s) 663, 773, 947
AlwI GGATC 3 cut(s) 56, 513, 515
AoxI GGCC 3 cut(s) 129, 332, 925
ApoI RAATTY 4 cut(s) 178, 296, 571, 884
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 546
Asp718I GGTACC 1 cut(s) 724
AspLEI GCGC 1 cut(s) 1017
AspS9I GGNCC 1 cut(s) 49
AvaII GGWCC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 724
BbsI GAAGAC 2 cut(s) 173, 822
BccI CCATC 1 cut(s) 79
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 2 cut(s) 12, 929
BciVI GTATCC 1 cut(s) 601
BcoDI GTCTC 3 cut(s) 663, 773, 947
BfaI CTAG 2 cut(s) 330, 783
BfuAI ACCTGC 1 cut(s) 645
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 2 cut(s) 18, 26
BlsI GCNGC 2 cut(s) 19, 27
Bme1390I CCNGG 2 cut(s) 12, 929
Bme18I GGWCC 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 264, 369, 726
BmrFI CCNGG 2 cut(s) 12, 929
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 4 cut(s) 312, 711, 955, 1013
BmuI ACTGGG 1 cut(s) 344
BpiI GAAGAC 2 cut(s) 173, 822
BplI GAGNNNNNCTC 2 cut(s) 643, 675
BpmI CTGGAG 1 cut(s) 1003
BpuEI CTTGAG 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 594
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 753, 871
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 350, 524
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 2 cut(s) 12, 929
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 3 cut(s) 35, 148, 309
BseLI CCNNNNNNNGG 3 cut(s) 211, 753, 871
BseMI GCAATG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 350, 524
BseRI GAGGAG 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 588
BshFI GGCC 3 cut(s) 131, 334, 927
BshNI GGYRCC 1 cut(s) 724
BsiSI CCGG 1 cut(s) 393
BslI CCNNNNNNNGG 3 cut(s) 211, 753, 871
BsmAI GTCTC 3 cut(s) 663, 773, 947
BsnI GGCC 3 cut(s) 131, 334, 927
Bso31I GGTCTC 1 cut(s) 663
Bsp143I GATC 3 cut(s) 61, 505, 520
BspACI CCGC 3 cut(s) 17, 26, 266
BspANI GGCC 3 cut(s) 131, 334, 927
BspFNI CGCG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 264, 369, 726
BspMI ACCTGC 1 cut(s) 645
BspPI GGATC 3 cut(s) 56, 513, 515
BspT107I GGYRCC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 2 cut(s) 350, 524
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 3 cut(s) 61, 505, 520
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 2 cut(s) 12, 929
Bst4CI ACNGT 2 cut(s) 454, 556
Bst6I CTCTTC 2 cut(s) 39, 759
BstACI GRCGYC 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 30, 970
BstDEI CTNAG 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 35, 148, 309
BstFNI CGCG 1 cut(s) 588
BstHHI GCGC 1 cut(s) 1017
BstKTI GATC 3 cut(s) 64, 508, 523
BstMAI GTCTC 3 cut(s) 663, 773, 947
BstMBI GATC 3 cut(s) 61, 505, 520
BstMWI GCNNNNNNNGC 2 cut(s) 128, 1023
BstNI CCWGG 2 cut(s) 12, 929
BstSCI CCNGG 2 cut(s) 10, 927
BstUI CGCG 1 cut(s) 588
BstV2I GAAGAC 2 cut(s) 173, 822
BstX2I RGATCY 3 cut(s) 61, 505, 520
BstYI RGATCY 3 cut(s) 61, 505, 520
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 3 cut(s) 131, 334, 927
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 3 cut(s) 35, 148, 309
BtsIMutI CAGTG 1 cut(s) 535
BveI ACCTGC 1 cut(s) 645
Cac8I GCNNGC 2 cut(s) 30, 970
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 1017
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 2 cut(s) 9, 479
CsiI ACCWGGT 1 cut(s) 10
Csp6I GTAC 4 cut(s) 202, 380, 725, 758
CviAII CATG 3 cut(s) 206, 274, 691
CviQI GTAC 4 cut(s) 202, 380, 725, 758
DdeI CTNAG 1 cut(s) 601
DpnI GATC 3 cut(s) 63, 507, 522
DpnII GATC 3 cut(s) 61, 505, 520
Eam1104I CTCTTC 2 cut(s) 39, 759
EarI CTCTTC 2 cut(s) 39, 759
Eco130I CCWWGG 1 cut(s) 594
Eco147I AGGCCT 2 cut(s) 131, 927
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 3 cut(s) 185, 714, 942
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoRII CCWGG 2 cut(s) 10, 927
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 3 cut(s) 209, 277, 694
FatI CATG 3 cut(s) 205, 273, 690
Fnu4HI GCNGC 2 cut(s) 18, 26
FokI GGATG 3 cut(s) 22, 155, 296
Fsp4HI GCNGC 2 cut(s) 18, 26
FspBI CTAG 2 cut(s) 330, 783
GlaI GCGC 1 cut(s) 1016
GluI GCNGC 2 cut(s) 18, 26
GsaI CCCAGC 1 cut(s) 505
GsuI CTGGAG 1 cut(s) 1003
HaeIII GGCC 3 cut(s) 131, 334, 927
HapII CCGG 1 cut(s) 393
HgaI GACGC 2 cut(s) 9, 479
HhaI GCGC 1 cut(s) 1017
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 3 cut(s) 209, 277, 694
Hin6I GCGC 1 cut(s) 1015
HinP1I GCGC 1 cut(s) 1015
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 5 cut(s) 95, 155, 290, 614, 997
HpaII CCGG 1 cut(s) 393
Hpy166II GTNNAC 2 cut(s) 465, 760
Hpy188I TCNGA 8 cut(s) 40, 154, 160, 342, 541, 565, 707, 1034
Hpy188III TCNNGA 7 cut(s) 59, 239, 274, 415, 662, 872, 1020
Hpy8I GTNNAC 2 cut(s) 465, 760
Hpy99I CGWCG 2 cut(s) 25, 235
HpyAV CCTTC 7 cut(s) 97, 142, 253, 442, 645, 738, 917
HpyCH4III ACNGT 2 cut(s) 454, 556
HpyCH4IV ACGT 2 cut(s) 233, 648
HpyCH4V TGCA 2 cut(s) 457, 739
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 1023
HpyF3I CTNAG 1 cut(s) 601
HpySE526I ACGT 2 cut(s) 233, 648
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 3 cut(s) 209, 277, 694
HspAI GCGC 1 cut(s) 1015
KpnI GGTACC 1 cut(s) 728
Kzo9I GATC 3 cut(s) 61, 505, 520
LmnI GCTCC 2 cut(s) 268, 1022
LweI GCATC 4 cut(s) 312, 711, 955, 1013
MabI ACCWGGT 1 cut(s) 10
MaeI CTAG 2 cut(s) 330, 783
MaeII ACGT 2 cut(s) 233, 648
MaeIII GTNAC 1 cut(s) 440
MalI GATC 3 cut(s) 63, 507, 522
MboI GATC 3 cut(s) 61, 505, 520
MfeI CAATTG 2 cut(s) 282, 372
MflI RGATCY 3 cut(s) 61, 505, 520
MluI ACGCGT 1 cut(s) 586
MlyI GAGTC 1 cut(s) 1006
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 6 cut(s) 40, 87, 121, 160, 370, 805
MroXI GAANNNNTTC 1 cut(s) 546
MseI TTAA 4 cut(s) 192, 569, 575, 975
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 2 cut(s) 12, 929
MunI CAATTG 2 cut(s) 282, 372
MvaI CCWGG 2 cut(s) 12, 929
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 2 cut(s) 128, 1023
NdeII GATC 3 cut(s) 61, 505, 520
NlaIII CATG 3 cut(s) 209, 277, 694
NlaIV GGNNCC 3 cut(s) 264, 369, 726
NmeAIII GCCGAG 1 cut(s) 66
PagI TCATGA 1 cut(s) 273
PceI AGGCCT 2 cut(s) 131, 927
PcsI WCGNNNNNNNCGW 1 cut(s) 768
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 4 cut(s) 95, 155, 290, 614
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 2 cut(s) 19, 27
PleI GAGTC 1 cut(s) 1005
PpsI GAGTC 1 cut(s) 1005
PshBI ATTAAT 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 10, 927
PspFI CCCAGC 1 cut(s) 501
PspGI CCWGG 2 cut(s) 10, 927
PspN4I GGNNCC 3 cut(s) 264, 369, 726
PspPI GGNCC 1 cut(s) 49
PsuI RGATCY 3 cut(s) 61, 505, 520
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 4 cut(s) 203, 381, 726, 759
RsaNI GTAC 4 cut(s) 202, 380, 725, 758
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 4 cut(s) 192, 569, 575, 975
SatI GCNGC 2 cut(s) 18, 26
Sau3AI GATC 3 cut(s) 61, 505, 520
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 1006
ScrFI CCNGG 2 cut(s) 12, 929
SexAI ACCWGGT 1 cut(s) 10
SfaNI GCATC 4 cut(s) 312, 711, 955, 1013
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SseBI AGGCCT 2 cut(s) 131, 927
SsiI CCGC 3 cut(s) 17, 26, 266
SspMI CTAG 2 cut(s) 330, 783
StuI AGGCCT 2 cut(s) 131, 927
StyD4I CCNGG 2 cut(s) 10, 927
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 2 cut(s) 454, 556
TaiI ACGT 2 cut(s) 236, 651
TaqI TCGA 4 cut(s) 230, 238, 416, 497
TauI GCSGC 2 cut(s) 20, 28
TfiI GAWTC 4 cut(s) 95, 155, 290, 614
Tru1I TTAA 4 cut(s) 192, 569, 575, 975
Tru9I TTAA 4 cut(s) 192, 569, 575, 975
TscAI CASTG 1 cut(s) 535
TspDTI ATGAA 6 cut(s) 17, 57, 264, 290, 627, 950
TspGWI ACGGA 4 cut(s) 155, 482, 659, 858
TspRI CASTG 1 cut(s) 535
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 49
VspI ATTAAT 1 cut(s) 192
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 4 cut(s) 178, 296, 571, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 2 cut(s) 330, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.